Studies on subcellular fractions which are involved in myelin assembly: labeling of myelin proteins by a double radioisotope approach indicates developmental relationships.

abstract：:Conversion of the cellular prion protein (PrP(C)) into the abnormal scrapie isoform (PrP(Sc)) is the hallmark of prion diseases, which are fatal and transmissible neurodegenerative disorders. ER-retained anti-prion recombinant single-chain Fv fragments have been proved to be an effective tool for inhibition of PrP(C) ...

journal_title：Journal of neurochemistry

authors： Filesi I,Cardinale A,Mattei S,Biocca S

更新日期：2007-06-01 00:00:00

abstract：:The content of the tryptophan metabolites quinolinic acid (QUIN), 5-hydroxytryptamine (5-HT), and 5-hydroxyindoleacetic acid (5-HIAA) was measured in various brain areas of rats bearing a portocaval anastomosis (PCA) for 4 weeks, using mass fragmentography or HPLC. In these animals, the content of the excitotoxic comp...

journal_title：Journal of neurochemistry

authors： Moroni F,Lombardi G,Carlà V,Pellegrini D,Carassale GL,Cortesini C

更新日期：1986-03-01 00:00:00

abstract：:1-[2-(4-Aminophenyl)ethyl]-4-(3-trifluoromethylphenyl)piperazine (PAPP) inhibits [3H]5-hydroxytryptamine (5-HT, serotonin) binding to 5-HT1A and 5-HT1B sites in rat brain with apparent equilibrium dissociation constants (KD) of 2.9 and 328 nM, respectively. [3H]PAPP was synthesized, its binding to central serotonin re...

journal_title：Journal of neurochemistry

authors： Ransom RW,Asarch KB,Shih JC

更新日期：1986-01-01 00:00:00

abstract：:We have introduced a series of point mutations into the human opioid receptor-like (ORL1) receptor and characterized them for their ability to constitutively activate G protein-coupled receptor signalling pathways. Among the 12 mutants generated, mutation at Asn133 (N133W) gave increased basal signalling through three...

journal_title：Journal of neurochemistry

authors： Kam KW,New DC,Wong YH

更新日期：2002-12-01 00:00:00

abstract：:Myelin P2 protein is a small (14,800 Da) protein found in peripheral and central nervous system myelin. To investigate the regulation of expression of this myelin protein, a mouse genomic library was screened with a rabbit P2 cDNA (pSN2.2-2), and a single positive phage clone containing a 20-kb insert was obtained. Th...

journal_title：Journal of neurochemistry

authors： Narayanan V,Kaestner KH,Tennekoon GI

更新日期：1991-07-01 00:00:00

abstract：:Mutations within the amyloid-beta (Abeta) domain of the amyloid precursor protein (APP) typically generate hemorrhagic strokes and vascular amyloid angiopathy. In contrast, the Arctic mutation (APP E693G) results in Alzheimer's disease. Little is known about the pathologic mechanisms that result from the Arctic mutati...

journal_title：Journal of neurochemistry

authors： Sahlin C,Lord A,Magnusson K,Englund H,Almeida CG,Greengard P,Nyberg F,Gouras GK,Lannfelt L,Nilsson LN

更新日期：2007-05-01 00:00:00

abstract：:The effects of fatty acids, oleate and palmitate, on gamma-aminobutyric acid (GABA), aspartate, and 3,4- dihydroxyphenylethylamine (dopamine) transport and a variety of other membrane functions were studied in rat brain synaptosomes at a constant lipid-to-protein ratio. Under the conditions utilized oleate, but not pa...

journal_title：Journal of neurochemistry

authors： Troeger MB,Rafalowska U,Erecińska M

更新日期：1984-06-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is a neurotrophic factor essential for maintenance of motor neurons. Loss of this factor produces a phenotype similar to amyotrophic lateral sclerosis (ALS). We recently showed that ALS-producing mutations of Cu/Zn-superoxide dismutase (SOD1) disrupt post-transcriptional regul...

journal_title：Journal of neurochemistry

authors： Li X,Lu L,Bush DJ,Zhang X,Zheng L,Suswam EA,King PH

更新日期：2009-02-01 00:00:00

abstract：:We have correlated myelin membrane structure with biochemical composition in the CNS and PNS of a phylogenetic series of animals, including elasmobranchs, teleosts, amphibians, and mammals. X-ray diffraction patterns were recorded from freshly dissected, unfixed tissue and used to determine the thicknesses of the liqu...

journal_title：Journal of neurochemistry

authors： Kirschner DA,Inouye H,Ganser AL,Mann V

更新日期：1989-11-01 00:00:00

abstract：:The molecular forms of angiotensin converting enzyme (ACE; EC 3.4.15.1) in preparations of pig brain cortical microvessels and striatal synaptosomal membranes have been identified by immunoelectrophoretic blot analysis. The cortical microvessels contained only the endothelial form of the enzyme, Mr 180,000, which comi...

journal_title：Journal of neurochemistry

authors： Williams TA,Hooper NM,Turner AJ

更新日期：1991-07-01 00:00:00

abstract：:Calmodulin regulated spectrin-associated protein 1 (CAMSAP1) is a vertebrate microtubule-binding protein, and a representative of a family of cytoskeletal proteins that arose with animals. We reported previously that the central region of the protein, which contains no recognized functional domain, inhibited neurite o...

journal_title：Journal of neurochemistry

authors： King MD,Phillips GW,Bignone PA,Hayes NV,Pinder JC,Baines AJ

更新日期：2014-02-01 00:00:00

abstract：:Protein aggregation into amyloid fibrils is a key feature of a multitude of neurodegenerative diseases such as Alzheimer's, Parkinson's, and Prion disease. To detect amyloid fibrils, fluorophores with high sensitivity and better efficiency coupled with the low toxicity are in high demand even to date. In this pursuit,...

journal_title：Journal of neurochemistry

authors： Pravin N,Kumar R,Tripathi S,Kumar P,Mohite GM,Navalkar A,Panigrahi R,Singh N,Gadhe LG,Manchanda S,Shimozawa M,Nilsson P,Johansson J,Kumar A,Maji SK,Shanmugam M

更新日期：2020-08-04 00:00:00

abstract：:PK 11195 [1-(2-chlorophenyl)-N-methyl-N-(1-methylpropyl)-3-isoquinolinecarboxamide] is a new ligand for the "peripheral-type" benzodiazepine binding sites, chemically unrelated to benzodiazepines. It displaces with a very high potency (IC50 congruent to 10(-9) M) [3H]-RO5-4864 (a benzodiazepine which specifically labe...

journal_title：Journal of neurochemistry

authors： Benavides J,Quarteronet D,Imbault F,Malgouris C,Uzan A,Renault C,Dubroeucq MC,Gueremy C,Le Fur G

更新日期：1983-12-01 00:00:00

abstract：:Manganese is an essential nutrient, integral to proper metabolism of amino acids, proteins and lipids. Excessive environmental exposure to manganese can produce extrapyramidal symptoms similar to those observed in Parkinson's disease (PD). We used in vivo and in vitro models to examine cellular and circuitry alteratio...

journal_title：Journal of neurochemistry

authors： Stanwood GD,Leitch DB,Savchenko V,Wu J,Fitsanakis VA,Anderson DJ,Stankowski JN,Aschner M,McLaughlin B

更新日期：2009-07-01 00:00:00

abstract：:The ontogeny of muscarinic receptors was studied in human fetal striatum, brainstem, and cerebellum to investigate general principles of synaptogenesis as well as the physiological balance between various chemical synapses during development in a given region of the brain. [3H]Quinuclidinyl benzilate ([3H]QNB) binding...

journal_title：Journal of neurochemistry

authors： Ravikumar BV,Sastry PS

更新日期：1985-12-01 00:00:00

abstract：:The glutamate receptor 2 (GluR2) subunit determines many of the functional properties of the alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate subtype of glutamate receptor. The roles of untranslated regions (UTRs) in mRNA stability, transport, or translation are increasingly recognized. The 3' end of the GluR2 tra...

journal_title：Journal of neurochemistry

authors： Irier HA,Shaw R,Lau A,Feng Y,Dingledine R

更新日期：2009-04-01 00:00:00

abstract：:Glial cells are currently viewed as active partners of neurons in synapse formation. The close proximity of astrocytes to the synaptic cleft suggests that these cells might be potential targets for neuronal-released molecules although this issue has been less addressed. Here, we evaluated the role of the excitatory ne...

journal_title：Journal of neurochemistry

authors： Romão LF,Sousa Vde O,Neto VM,Gomes FC

更新日期：2008-07-01 00:00:00

abstract：:Our gut harbours trillions of microorganisms essential for the maintenance of homeostasis and host physiology in health and disease. In the last decade, there has been a growing interest in understanding the bidirectional pathway of communication between our microbiota and the central nervous system. With regard to re...

journal_title：Journal of neurochemistry

authors： García-Cabrerizo R,Carbia C,O Riordan KJ,Schellekens H,Cryan JF

更新日期：2020-12-25 00:00:00

abstract：:We have characterized the structural properties of heparan sulfates from brain and other tissues after depolymerization with a mixture of three heparin and heparan sulfate lyases from Flavobacterium heparinum. The resulting disaccharides were separated by HPLC and identified by comparison with authentic standards. In ...

journal_title：Journal of neurochemistry

authors： Tekotte H,Engel M,Margolis RU,Margolis RK

更新日期：1994-03-01 00:00:00

abstract：:Ischemic stroke is a neurovascular disease treatable by thrombolytic therapy, but the therapy has to be initiated within 3 h of the incident. This therapeutic limitation stems from the secondary injury which results mainly from oxidative stress and inflammation. A potent antioxidant/anti-inflammatory agent, caffeic ac...

journal_title：Journal of neurochemistry

authors： Khan M,Elango C,Ansari MA,Singh I,Singh AK

更新日期：2007-07-01 00:00:00

abstract：:We applied our in vivo fatty acid method to examine concentrations, incorporation, and turnover rates of docosahexaenoic acid (22:6 n-3) in brains of rats subject to a dietary deficiency of alpha-linolenic acid (18:3 n-3) for three generations. Adult deficient and adequate rats of the F3 generation were infused intrav...

journal_title：Journal of neurochemistry

authors： Contreras MA,Greiner RS,Chang MC,Myers CS,Salem N Jr,Rapoport SI

更新日期：2000-12-01 00:00:00

abstract：:The effects of muscarinic agonists and depolarizing agents on inositol phospholipid hydrolysis in the rabbit vagus nerve were assessed by the measurement of [3H]inositol monophosphate production in nerves that had been preincubated with [3H]inositol. After 1 h of drug action, carbachol, oxotremorine, and arecoline inc...

journal_title：Journal of neurochemistry

authors： Sierro CD,Vitus J,Dunant Y

更新日期：1992-08-01 00:00:00

abstract：:The novel N-methyl-D-aspartate receptor channel ligand (+)-[3H]5-methyl-10,11-dihydro-5H-dibenzo[a,d]-cyclohepten-5, 10-imine maleate ([3H]MK-801) has been utilized to label this receptor in human brain tissue. Characteristics of [3H]MK-801 binding to well-washed membranes from 17 control subjects and 16 patients with...

journal_title：Journal of neurochemistry

authors： Procter AW,Wong EH,Stratmann GC,Lowe SL,Bowen DM

更新日期：1989-09-01 00:00:00

abstract：:Highly purified noradrenergic, large, dense-cored vesicles were isolated from bovine sympathetic nerve endings by sucrose-D2O density gradient centrifugation. Their concentration of glycoprotein hexosamine and sialic acid was 6.6 and 3.9 mumol/100 mg lipid-free dry weight, respectively, values which are similar to tho...

journal_title：Journal of neurochemistry

authors： Margolis RU,Ledeen RW,Sbaschnig-Agler M,Byrne MC,Klein RL,Douglas BH 2nd,Margolis RK

更新日期：1987-12-01 00:00:00

abstract：:Mouse monoclonal B-50 antibodies (Mabs) were screened to select a Mab that may interfere with suggested functions of B-50 (GAP-43), such as involvement in neurotransmitter release. Because the Mab NM2 reacted with peptide fragments of rat B-50 containing the unique protein kinase C (PKC) phosphorylation site at serine...

journal_title：Journal of neurochemistry

authors： Oestreicher AB,Hens JJ,Marquart A,Mercken M,De Graan PN,Zwiers H,Gipsen WH

更新日期：1994-03-01 00:00:00

abstract：:Glutamate is the predominant excitatory neurotransmitter in the mammalian central nervous system and extracellular glutamate levels are controlled by a family of transporters known as excitatory amino acid transporters (EAATs). The EAATs transport glutamate and aspartate with similar micromolar affinities and this tra...

journal_title：Journal of neurochemistry

authors： Ryan RM,Kortt NC,Sirivanta T,Vandenberg RJ

更新日期：2010-07-01 00:00:00

abstract：:A sustained increase in the brain concentrations of neuroactive steroids was previously shown to induce Y1 receptor gene expression in the amygdala of Y1R/LacZ transgenic mice which harbour a construct comprising the murine Y1 receptor gene promoter and the lacZ reporter gene. We now investigated the effects of restra...

journal_title：Journal of neurochemistry

authors： Mele P,Oberto A,Serra M,Pisu MG,Floris I,Biggio G,Eva C

更新日期：2004-06-01 00:00:00

abstract：:Exposure to excessive levels of light induces photoreceptor apoptosis and can be a causative factor in age-related macular degeneration (AMD). However, the cellular events that mediate this apoptotic response are poorly understood. Here, we investigated the roles of endoplasmic reticulum (ER) stress in light-induced c...

journal_title：Journal of neurochemistry

authors： Nakanishi T,Shimazawa M,Sugitani S,Kudo T,Imai S,Inokuchi Y,Tsuruma K,Hara H

更新日期：2013-04-01 00:00:00

abstract：:Inducible expression of the group-I metabotropic glutamate receptor (mGlu1alpha) in Chinese hamster ovary cells allows for the study of receptor density dependent effects. However, expression levels attainable with this system are lower than those reported for various brain regions and achieved by conventional (consti...

journal_title：Journal of neurochemistry

authors： Nash MS,Selkirk JV,Gaymer CE,Challiss RA,Nahorski SR

更新日期：2001-06-01 00:00:00

abstract：:We have proposed that a very early step in the pathogenesis of idiopathic Parkinson's disease is the elevated translocation of L-cysteine into neuromelanin-pigmented dopaminergic neurons in the substantia nigra. This influx of L-cysteine was proposed to divert the normal neuromelanin pathway by scavenging dopamine-o-q...

journal_title：Journal of neurochemistry

authors： Li H,Shen XM,Dryhurst G

更新日期：1998-11-01 00:00:00