Emergence of a Synergistic Diversity as a Response to Competition in Pseudomonas putida Biofilms.

Abstract:

:Genetic diversification through the emergence of variants is one of the known mechanisms enabling the adaptation of bacterial communities. We focused in this work on the adaptation of the model strain Pseudomonas putida KT2440 in association with another P. putida strain (PCL1480) recently isolated from soil to investigate the potential role of bacterial interactions in the diversification process. On the basis of colony morphology, three variants of P. putida KT2440 were obtained from co-culture after 168 h of growth whereas no variant was identified from the axenic KT2440 biofilm. The variants exhibited distinct phenotypes and produced biofilms with specific architecture in comparison with the ancestor. The variants better competed with the P. putida PCL1480 strain in the dual-strain biofilms after 24 h of co-culture in comparison with the ancestor. Moreover, the synergistic interaction of KT2440 ancestor and the variants led to an improved biofilm production and to higher competitive ability versus the PCL1480 strain, highlighting the key role of diversification in the adaptation of P. putida KT2440 in the mixed community. Whole genome sequencing revealed mutations in polysaccharides biosynthesis protein, membrane transporter, or lipoprotein signal peptidase genes in variants.

journal_name

Microb Ecol

journal_title

Microbial ecology

authors

Bridier A,Piard JC,Briandet R,Bouchez T

doi

10.1007/s00248-019-01470-z

subject

Has Abstract

pub_date

2020-07-01 00:00:00

pages

47-59

issue

1

eissn

0095-3628

issn

1432-184X

pii

10.1007/s00248-019-01470-z

journal_volume

80

pub_type

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