Efficacy of zosteric acid sodium salt on the yeast biofilm model Candida albicans.

Abstract:

:Candida albicans is the most notorious and the most widely studied yeast biofilm former. Design of experiments (DoE) showed that 10 mg/L zosteric acid sodium salt reduced C. albicans adhesion and the subsequent biofilm formation by at least 70%, on both hydrophilic and hydrophobic surfaces of 96-well plates. Indeed, biofilm imaging revealed the dramatic impact of zosteric acid sodium salt on biofilm thickness and morphology, due to the inability of the cells to form filamentous structures while remaining metabolically active. In the same way, 10 mg/L zosteric acid sodium salt inhibited C. albicans biofilm formation when added after the adhesion phase. Contrary to zosteric acid sodium salt, methyl zosterate did not affect yeast biofilm. In addition, zosteric acid sodium salt enhanced sensitivity to chlorhexidine, chlorine, hydrogen peroxide, and cis-2-decenoic acid, with a reduction of 0.5 to 8 log units. Preliminary in vitro studies using suitable primary cell based models revealed that zosteric acid sodium salt did not compromise the cellular activity, adhesion, proliferation or morphology of either the murine fibroblast line L929 or the human osteosarcoma line MG-63. Thus the use of zosteric acid sodium salt could provide a suitable, innovative, preventive, and integrative approach to preventing yeast biofilm formation.

journal_name

Microb Ecol

journal_title

Microbial ecology

authors

Villa F,Pitts B,Stewart PS,Giussani B,Roncoroni S,Albanese D,Giordano C,Tunesi M,Cappitelli F

doi

10.1007/s00248-011-9876-x

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

584-98

issue

3

eissn

0095-3628

issn

1432-184X

journal_volume

62

pub_type

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