Abstract：
:At Iron Mountain, CA, there is an extreme occurrence of acid mine drainage (AMD). This is a result of past mining activity that has exposed a sulfide ore body to weathering and microbial activity. This study presents seven new oligonucleotide probes for the detection of microorganisms at this AMD site by fluorescent in situ hybridization. In the design of these probes we have accounted for a large body of 16S rRNA sequence data recently compiled by us. This was obtained by PCR and cloning directly from environmental DNA and was mostly represented by novel sequences. The probes were developed to include detection of novel and uncultivated organisms. This includes detection for the Thermoplasmales group, a new group of Leptospirillum, the genus Sulfobacillus, the Acidiphilium genus, Acidimicrobium and relatives, and for organisms within the delta Proteobacteria. These probes have been used to examine the abundance and distribution of organisms, including novel and uncultivated taxa, and to clarify their potential contributions to AMD production at the site. We anticipate that these probes will be useful tools for exploration of the microbiology of other natural acidic environments and bioleaching systems.
journal_name
Microb Ecoljournal_title
Microbial ecologyauthors
Bond PL,Banfield JFdoi
10.1007/s002480000063keywords:
subject
Has Abstractpub_date
2001-02-01 00:00:00pages
149-161issue
2eissn
0095-3628issn
1432-184Xjournal_volume
41pub_type
杂志文章abstract：:Atmospheric pollution has become a major problem for modern societies owing to its fatal effects on both human health and ecosystems. We studied the relationships of nitrogen dioxide atmospheric pollution and metal trace elements contained in atmospheric particles which were accumulated in bryophytes to microbial comm...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-009-9580-2
更新日期：2010-02-01 00:00:00
abstract：:Bisphenol A (BPA) is one of the endocrine-disrupting chemicals that are ubiquitous in aquatic environments. Biodegradation is a major way to clean up the BPA pollution in sediments. However, information on the effective BPA biodegradation in anaerobic sediments is still lacking. The present study investigated the biod...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-014-0551-x
更新日期：2015-07-01 00:00:00
abstract：:Biological soil crust is composed of lichens, cyanobacteria, green algae, mosses, and fungi. Although crusts are a dominant source of nitrogen (N) in arid ecosystems, this study is among the first to demonstrate their contribution to N availability in xeric temperate habitats. The study site is located in Lucas County...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-005-0121-3
更新日期：2006-02-01 00:00:00
abstract：:Growth kinetics of 2Nitrobacter strains (N.w. and L) coexisting in the same soil are studied in batch and continuous culture. Monod's parameters are estimated numerically from experimental data in the case of the batch experiment, and from steady-state equations in the case of the chemostat. In both cases, the 2 strai...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02011417
更新日期：1984-06-01 00:00:00
abstract：:The quantitative uptake of latex beads of different sizes and of live cells by 14 species of ciliates was studied. The functional response (uptake rate as function of food particle concentration) can be fitted to a hyperbolic function and this can be explained in terms of the function of the mouth apparatus. Each spec...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02020370
更新日期：1980-03-01 00:00:00
abstract：:Owing to their ubiquitous presence and ability to act as primary or opportunistic pathogens, Vibrio species greatly contribute to the diversity and evolution of marine ecosystems. This study was aimed at unveiling the cellular strategies enabling the marine gammaproteobacterium Vibrio harveyi to adapt and persist in n...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-015-0614-7
更新日期：2015-10-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-008-9406-7
更新日期：2009-04-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-016-0916-4
更新日期：2017-07-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-002-1059-3
更新日期：2003-05-01 00:00:00
abstract：:Symbioses between prokaryotes and microbial eukaryotes, particularly ciliated protists, have been studied for a long time. Nevertheless, researchers have focused only on a few host genera and species, mainly due to difficulties in cultivating the hosts, and usually have considered a single symbiont at a time. Here, we...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-018-1279-9
更新日期：2019-07-01 00:00:00
abstract：:Scleractinian corals form the framework of coral reefs and host abundant and diverse microbial communities that are fundamental to their success. A very limited number of studies have examined the co-occurrence of multiple partners within the coral 'holobiont' and their pattern of specificity over different geographic...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-018-1255-4
更新日期：2019-04-01 00:00:00
abstract：:Different experiments have estimated that the contribution of biological nitrogen fixation (BNF) is largely variable among sugarcane cultivars. Which bacteria are the most important in sugarcane-associated BNF is unknown. However, Gluconacetobacter diazotrophicus has been suggested as a strong candidate responsible fo...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-003-0110-3
更新日期：2003-11-01 00:00:00
abstract：:The present study aimed to experimentally quantify the influence of a reduction of surface sediment permeability on microbial characteristics and ecological processes (respiration and leaf litter decomposition) occurring in the hyporheic zone (i.e. the sedimentary interface between surface water and groundwater). The ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-010-9774-7
更新日期：2011-05-01 00:00:00
abstract：:Nickel (Ni) toxicity to growth and photosynthesis was studied in four strains of Scenedesmus acutus f. alternans. Effects of Ni dosage and duration of exposure on growth and photosynthesis were strain specific. Large differences in responses of both growth and photosynthesis to Ni were detected between three resistant...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF00175079
更新日期：1996-01-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-009-9614-9
更新日期：2010-01-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02018912
更新日期：1988-09-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-015-0624-5
更新日期：2015-11-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02019021
更新日期：1983-12-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02539153
更新日期：1991-12-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-020-01523-8
更新日期：2020-10-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-007-9309-z
更新日期：2008-05-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02013272
更新日期：1977-12-01 00:00:00
abstract：:Biological soil crusts cover hundreds of hectares of sand dunes at the northern tip of Cape Cod National Seashore (Massachusetts, USA). Although the presence of crusts in this habitat has long been recognized, neither the organisms nor their ecological roles have been described. In this study, we report on the microbi...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-004-0254-9
更新日期：2004-08-01 00:00:00
abstract：:The effect of carbon∶nitrogen∶phosphorus (C∶N∶P) ratio of organic substrates on the regeneration of ammonium and phosphate was investigated by growing natural assemblages of freshwater bacteria in mineral media supplemented with the simple organic C, N, and P sources (glucose, asparagine, and sodium glycerophosphate, ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02017167
更新日期：1990-05-01 00:00:00
abstract：:The dermis of cetaceans is in constant contact with microbial species. Although the skin of the bottlenose dolphin provides adequate defense against most disease-causing microbes, it also provides an environment for microbial community development. Microbial community uniqueness and richness associated with bottlenose...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-017-1071-2
更新日期：2018-02-01 00:00:00
abstract：:Biological soil crusts (BSCs) are communities of cryptogamic organisms, occurring in arid and semiarid regions all over the world. Based on both morphological identification and genetic analyses, we established a first cyanobacterial inventory using the biphasic approach for BSCs within two major biomes of southern Af...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-013-0301-5
更新日期：2014-02-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-011-9923-7
更新日期：2012-02-01 00:00:00
abstract：:Microbial biofilms in rivers contribute to the decomposition of the available organic matter which typically shows changes in composition and bioavailability due to their origin, seasonality, and watershed characteristics. In the context of global warming, enhanced biofilm organic matter decomposition would be expecte...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-012-0062-6
更新日期：2012-10-01 00:00:00
abstract：:Arcellininids (testate amoebae) were examined from 61 surface sediment samples collected from 59 lakes in the vicinity of former gold mines, notably Giant Mine, near Yellowknife, Northwest Territories, Canada to determine their utility as bioindicators of arsenic (As), which occurs both as a byproduct of gold extracti...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-016-0752-6
更新日期：2016-07-01 00:00:00
abstract：:The original article contained mistakes in the authors' affiliations. ...
journal_title：Microbial ecology
pub_type： 已发布勘误
doi：10.1007/s00248-020-01641-3
更新日期：2020-11-20 00:00:00
