Reliability of muramic acid as a bacterial biomarker is influenced by methodological artifacts from streptomycin.

Abstract:

:The muramic acid (MurA) assay is a powerful tool for the detection and quantification of bacteria with no need to enrich samples by culturing. However, the analysis of MurA in mixed biological and environmental matrices is potentially more complex than analysis in isolated bacterial cells. In this study, we employed one commonly used procedure for extraction of MurA from environmental samples and found that the presence of streptomycin interfered with the determination of MurA by creating chemical species that coeluted with the aldononitrile derivative of MurA prepared in this method. On a molar basis, streptomycin yields a signal that is approximately 0.67 times that of MurA. Mass spectrometry analysis confirmed that the interference from hydrolyzed streptomycin is not actually by MurA, but rather is likely to be N-methyl glucosamine. Because streptomycin is widely applied for selective growth of eukaryotes both in situ and in vitro, our findings may have implications for the significance of results from MurA assays. We conclude that MurA remains an effectual bacterial biomarker due to its unique bacterial origin, but care must be applied in interpreting results from the assay when performed in the presence of streptomycin.

journal_name

Microb Ecol

journal_title

Microbial ecology

authors

Liang C,Read HW,Balser TC

doi

10.1007/s00248-008-9406-7

subject

Has Abstract

pub_date

2009-04-01 00:00:00

pages

494-500

issue

3

eissn

0095-3628

issn

1432-184X

journal_volume

57

pub_type

杂志文章
  • Inhibition of the predatory activity ofBdellovibrio by various environmental pollutants.

    abstract::The predatory activity of bdellovibrios is affected by various environmental pollutants such as detergents, heavy metals, and pesticides. This was shown in a two-membered system ofBdellovibrio andPhotobacterium, in which the effect of the predator on the bioluminescence of the prey indicated the activity of the former...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02032492

    authors: Varon M,Shilo M

    更新日期:1981-06-01 00:00:00

  • Ecology of stem-nodulating Rhizobium and Azorhizobium in four vegetation zones of Senegal.

    abstract::The occurrence and distribution of Azorhizobium and Rhizobium strains that induce stem nodulation of Sesbania rostrata were determined in four vegetation zones in Senegal. Based on tests with 16 Rhizobium and 10 Azorhizobium strains nodulating S. rostrata, a method was devised to distinguish among the strains. In all ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF00217424

    authors: Robertson BK,Dreyfus B,Alexander M

    更新日期:1995-01-01 00:00:00

  • Molecular analyses of the microbial community composition of an anoxic basin of a municipal wastewater treatment plant reveal a novel lineage of proteobacteria.

    abstract::A culture-independent molecular phylogenetic approach was used to study prokaryotic diversity in an anoxic activated sludge from a municipal wastewater treatment plant. Two 16S rRNA gene libraries were constructed using total genomic DNA and amplified by polymerase chain reaction using primers specific for archaeal or...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-009-9632-7

    authors: Chouari R,Le Paslier D,Daegelen P,Dauga C,Weissenbach J,Sghir A

    更新日期:2010-08-01 00:00:00

  • Dynamics of Abundant and Rare Bacteria During Degradation of Lignocellulose from Sugarcane Biomass.

    abstract::Microorganisms play a crucial role in lignocellulosic degradation. Many enriched microbial communities have demonstrated to reach functional and structural stability with effective degrading capacities of industrial interest. These microbial communities are typically composed by only few dominant species and a high nu...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-019-01403-w

    authors: Puentes-Téllez PE,Salles JF

    更新日期:2020-02-01 00:00:00

  • Growth responses of ciliate protozoa to the abundance of their bacterial prey.

    abstract::The growth rate or numerical response of five species of bactivorous ciliates to the abundance ofEnterobacter aerogenes was examined in monoxenic culture. The ciliatesColpidium campylum, C. colpoda, Glaucoma scintillons, G. frontata, andCyclidium glaucoma were isolated from a small pond. Four were grown in shaken cult...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02015077

    authors: Taylor WD

    更新日期:1977-09-01 00:00:00

  • Trophic Ecology of the Tropical Pacific Sponge Mycale grandis Inferred from Amino Acid Compound-Specific Isotopic Analyses.

    abstract::Many sponges host abundant and active microbial communities that may play a role in the uptake of dissolved organic matter (DOM) by the sponge holobiont, although the mechanism of DOM uptake and metabolism is uncertain. Bulk and compound-specific isotopic analysis of whole sponge, isolated sponge cells, and isolated s...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-019-01410-x

    authors: Shih JL,Selph KE,Wall CB,Wallsgrove NJ,Lesser MP,Popp BN

    更新日期:2020-02-01 00:00:00

  • Viable real-time PCR in environmental samples: can all data be interpreted directly?

    abstract::Selective nucleic acid intercalating dyes--ethidium monoazide (EMA) and propidium monoazide (PMA)--represent one of the most successful recent approaches to detect viable cells (as defined by an intact cell membrane) by PCR and have been effectively evaluated in different microorganisms. However, some practical limita...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-010-9719-1

    authors: Fittipaldi M,Codony F,Adrados B,Camper AK,Morató J

    更新日期:2011-01-01 00:00:00

  • Unveiling the Metabolic Pathways Associated with the Adaptive Reduction of Cell Size During Vibrio harveyi Persistence in Seawater Microcosms.

    abstract::Owing to their ubiquitous presence and ability to act as primary or opportunistic pathogens, Vibrio species greatly contribute to the diversity and evolution of marine ecosystems. This study was aimed at unveiling the cellular strategies enabling the marine gammaproteobacterium Vibrio harveyi to adapt and persist in n...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-015-0614-7

    authors: Kaberdin VR,Montánchez I,Parada C,Orruño M,Arana I,Barcina I

    更新日期:2015-10-01 00:00:00

  • The ecology of the yeast flora in necroticOpuntia cacti and of associatedDrosophila in Australia.

    abstract::A survey was made of the yeast communities isolated from necrotic tissue of 4 species of prickly-pear cacti (Opuntia stricta, O. tomentosa, O. monacantha, andO. streptacantha) which have colonized in Australia. Yeast communities were sampled from a number of localities and at different times. Cactus specific yeasts ac...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02015562

    authors: Barker JS,East PD,Phaff HJ,Miranda M

    更新日期:1984-12-01 00:00:00

  • Large differences in the fraction of active bacteria in plankton, sediments, and biofilm.

    abstract::Generally, only a small fraction of free-living pelagic bacteria are metabolically active, while particle-associated bacteria usually exhibit a larger proportion of active bacteria. Most previous studies on the active fraction of bacteria focus on planktonic communities, and there are only a few studies on sediment an...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-002-2005-0

    authors: Haglund AL,Törnblom E,Boström B,Tranvik L

    更新日期:2002-03-01 00:00:00

  • Microbial Community Enhances Biodegradation of Bisphenol A Through Selection of Sphingomonadaceae.

    abstract::Bisphenol A (BPA) is a common ingredient in plastic wares and epoxy resins that are essential for our daily life. Despite the obvious benefits, BPA may act as an environmental endocrine disruptor, causing metabolic, reproductive, and/or developmental consequences and diseases in humans and other organisms. Although pr...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-018-1263-4

    authors: Oh S,Choi D

    更新日期:2019-04-01 00:00:00

  • Dysbiosis signature of fecal microbiota in colorectal cancer patients.

    abstract::The human gut microbiota is a complex system that is essential to the health of the host. Increasing evidence suggests that the gut microbiota may play an important role in the pathogenesis of colorectal cancer (CRC). In this study, we used pyrosequencing of the 16S rRNA gene V3 region to characterize the fecal microb...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-013-0245-9

    authors: Wu N,Yang X,Zhang R,Li J,Xiao X,Hu Y,Chen Y,Yang F,Lu N,Wang Z,Luan C,Liu Y,Wang B,Xiang C,Wang Y,Zhao F,Gao GF,Wang S,Li L,Zhang H,Zhu B

    更新日期:2013-08-01 00:00:00

  • Association of rumen ciliate populations with plant particles in vitro.

    abstract::Seven known species of rumen ciliates and mixedEntodinium spp. showed association with plant particles in rumen fluid in vitro. Association was greater with fresh particles than with hay, and substantially decreased when the water-soluble components of the particles were removed, suggesting that the water-soluble comp...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02015109

    authors: Orpin CG

    更新日期:1985-03-01 00:00:00

  • Analysis of spatial and temporal variation in the community structure of yeasts associated with decayingOpuntia cactus.

    abstract::The microbial structure within, between, and over time in decaying cladodes of the common prickly pearOpuntia stricta was studied at each of two separate localities. In general, the effective number of yeast species and yeast species diversity increased as the rot aged to the observed maximum time of 4 weeks. Yeast he...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02012946

    authors: Barker JS,Starmer WT,Vacek DC

    更新日期:1987-11-01 00:00:00

  • Protection of oilseed rape (Brassica napus) toward fungal pathogens by strains of plant-associated Bacillus amyloliquefaciens.

    abstract::In this report, four Bacillus strains were tested for effects on plant fitness and disease protection of oilseed rape (Brassica napus). The strains belonged to newly discovered plant-associated Bacillus amyloliquefaciens and a recently proposed species, Bacillus endophyticus. The fungal pathogens tested represented di...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-006-9181-2

    authors: Danielsson J,Reva O,Meijer J

    更新日期:2007-07-01 00:00:00

  • Moving Waves of Bacterial Populations and Total Organic Carbon along Roots of Wheat.

    abstract::> Abstract To determine if spatial variation in soluble carbon sources along the root coincides with different trophic groups of bacteria, copiotrophic and oligotrophic bacteria were enumerated from bulk soil and rhizosphere samples at 2 cm intervals along wheat roots 2, 3, and 4 weeks after planting. There was a mode...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s002489900136

    authors: Semenov AM,van Bruggen AHC,Zelenev VV

    更新日期:1999-02-01 00:00:00

  • Carbon Cycling: Molecular Regulation of Photosynthetic Carbon Fixation

    abstract::Photosynthetic carbon fixation by phytoplankton is a key component of the global carbon cycle. Our understanding of the types of picoplankton and ultraphytoplankton involved in this process is evolving. However, mechanisms of regulation of photosynthetic carbon fixation in the oceans are poorly understood. All phytopl...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF00183060

    authors: Paul JH

    更新日期:1996-11-01 00:00:00

  • Genotypic and phenotypic diversity of cyanobacteria in biological soil crusts of the Succulent Karoo and Nama Karoo of southern Africa.

    abstract::Biological soil crusts (BSCs) are communities of cryptogamic organisms, occurring in arid and semiarid regions all over the world. Based on both morphological identification and genetic analyses, we established a first cyanobacterial inventory using the biphasic approach for BSCs within two major biomes of southern Af...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-013-0301-5

    authors: Dojani S,Kauff F,Weber B,Büdel B

    更新日期:2014-02-01 00:00:00

  • Fermentation Ability of Gut Microbiota of Wild Japanese Macaques in the Highland and Lowland Yakushima: In Vitro Fermentation Assay and Genetic Analyses.

    abstract::Wild Japanese macaques (Macaca fuscata Blyth) living in the highland and lowland areas of Yakushima are known to have different diets, with highland individuals consuming more leaves. We aim to clarify whether and how these differences in diet are also reflected by gut microbial composition and fermentation ability. T...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-020-01515-8

    authors: Hanya G,Tackmann J,Sawada A,Lee W,Pokharel SS,de Castro Maciel VG,Toge A,Kuroki K,Otsuka R,Mabuchi R,Liu J,Hatakeyama M,Yamasaki E,von Mering C,Shimizu-Inatsugi R,Hayakawa T,Shimizu KK,Ushida K

    更新日期:2020-08-01 00:00:00

  • Microbial-invertebrate interactions and potential for biotechnology.

    abstract:CONCLUSION:As the interactions between marine invertebrates and their bacterial commensals and symbionts are better understood, the application of biotechnology will enhance both environmental and economic benefit. In the immediate future, marine bacteria, either selected or genetically engineered, will play a signific...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02153225

    authors: Bonar DB,Weiner RM,Colwell RR

    更新日期:1986-03-01 00:00:00

  • Distribution of epiphytic bacteria on olive leaves and the influence of leaf age and sampling time.

    abstract::Mesophilic heterotrophic, aerobic or facultatively anaerobic bacteria that grow on yeast tryptone glucose extract agar were isolated from the surface of olive leaves of 3 or 4 different ages in January, April, July, and October from 1984 to 1989. Unweighted average linkage cluster analysis on either the Jaccard coeffi...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02539143

    authors: Ercolani GL

    更新日期:1991-12-01 00:00:00

  • Protozoan grazing of bacteria in soil-impact and importance.

    abstract::Interactions between bacteria and protozoa in soil were studied over 2-week periods in the field and in a pot experiment. Under natural conditions the total biological activity was temporarily synchronized by a large rainfall, and in the laboratory by the addition of water to dried-out soil, with or without plants. In...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02341429

    authors: Clarholm M

    更新日期:1981-12-01 00:00:00

  • Bacterial diversity in the cecum of the world's largest living rodent (Hydrochoerus hydrochaeris).

    abstract::The capybara (Hydrochoerus hydrochaeris) is the world's largest living rodent. Native to South America, this hindgut fermenter is herbivorous and coprophagous and uses its enlarged cecum to digest dietary plant material. The microbiota of specialized hindgut fermenters has remained largely unexplored. The aim of this ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-011-9963-z

    authors: García-Amado MA,Godoy-Vitorino F,Piceno YM,Tom LM,Andersen GL,Herrera EA,Domínguez-Bello MG

    更新日期:2012-05-01 00:00:00

  • Phosphorus Input Alters the Assembly of Rice (Oryza sativa L.) Root-Associated Communities.

    abstract::Rice root-associated microbial community play an important role in plant nutrient acquisition, biomass production, and stress tolerance. Herein, root-associated community assembly was investigated under different phosphate input levels in phosphorus (P)-deficient paddy soil. Rice was grown in a long-term P-depleted pa...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-019-01407-6

    authors: Long XE,Yao H

    更新日期:2020-02-01 00:00:00

  • Measurement of Electron Transport System (ETS) activity in soil.

    abstract::Electron transport system (ETS) activity was measured in amended and nonamended soil by measuring the reduction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT) to iodonitrotetrazolium formazan (INT-formazan), which can be easily extracted with methanol without interference from other compound...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02010449

    authors: Trevors JT,Mayfield CI,Inniss WE

    更新日期:1982-10-01 00:00:00

  • Symbiont-Mediated Host-Parasite Dynamics in a Fungus-Gardening Ant.

    abstract::Group-living can promote the evolution of adaptive strategies to prevent and control disease. Fungus-gardening ants must cope with two sets of pathogens, those that afflict the ants themselves and those of their symbiotic fungal gardens. While much research has demonstrated the impact of specialized fungal pathogens t...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-017-1124-6

    authors: Kellner K,Kardish MR,Seal JN,Linksvayer TA,Mueller UG

    更新日期:2018-08-01 00:00:00

  • Species diversity, distribution, and genetic structure of endophytic and epiphytic Trichoderma associated with banana roots.

    abstract::Selective isolation, molecular identification and AFLP were used to investigate the distribution of the various species of endophytic and epiphytic Trichoderma associated with banana roots and to compare and contrast their genetic structure. Three specific groups of Trichoderma were observed in the roots of banana. Gr...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-010-9770-y

    authors: Xia X,Lie TK,Qian X,Zheng Z,Huang Y,Shen Y

    更新日期:2011-04-01 00:00:00

  • Quantifying Ostreid herpesvirus (OsHV-1) genome copies and expression during transmission.

    abstract::Understanding the pathogenic potential of a new pathogen strain or a known pathogen in a new locale is crucial for management of disease in both wild and farmed animals. The Ostreid herpesvirus-1 (OsHV-1), a known pathogen of early-life-stage Pacific oysters, Crassostrea gigas, has been associated with mortalities of ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-011-9937-1

    authors: Burge CA,Friedman CS

    更新日期:2012-04-01 00:00:00

  • Bacterial Growth Rate and Marine Virus-Host Dynamics.

    abstract::The dynamics of a marine virus-host system were investigated at different steady state growth rates in chemostat cultures and the data were analyzed using a simple model. The virus-host interactions showed strong dependence on host cell growth rate. The duration of the infection cycle and the virus burst size were fou...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s002480000050

    authors: Middelboe M

    更新日期:2000-08-01 00:00:00

  • Cell Density Effects of Frog Skin Bacteria on Their Capacity to Inhibit Growth of the Chytrid Fungus, Batrachochytrium dendrobatidis.

    abstract::Bacterial symbionts on frog skin can reduce the growth of the chytrid fungus Batrachochytrium dendrobatidis (Bd) through production of inhibitory metabolites. Bacteria can be effective at increasing the resistance of amphibians to chytridiomycosis when added to amphibian skin, and isolates can be screened for producti...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-015-0701-9

    authors: Yasumiba K,Bell S,Alford R

    更新日期:2016-01-01 00:00:00