Abstract：
:The 16S rDNA genes of an apparently pure culture of a psychrophilic and strict barophilic bacterium (WHB 46) were studied by PCR-mediated amplification and cloning into phage M13 mp18. Sequence analysis of five individual clones revealed the presence of two different 16S rDNA types. The homology value of 90% indicates that culture WHB 46 is actually composed of two closely related species (WHB 46-1 and 46-2). Both strains are members of the γ-subdivision of proteobacteria. Analysis of a sixth clone (WHB 46-1/2) leads to the conclusion that it represents a 16S rDNA hybrid molecule assembled during the PCR reaction. This hypothesis was confirmed by secondary structure analysis of the chimeric rDNA. The appearance of such hybrid molecules point to a potential risk in studies on the diversity of bacterial populations by analysis of rDNA pattern via PCR-mediated amplification because they suggest the existence of organisms that do not actually exist in the sample investigated.
journal_name
Microb Ecoljournal_title
Microbial ecologyauthors
Liesack W,Weyland H,Stackebrandt Edoi
10.1007/BF02539153subject
Has Abstractpub_date
1991-12-01 00:00:00pages
191-8issue
1eissn
0095-3628issn
1432-184Xjournal_volume
21pub_type
杂志文章abstract：:Numerous studies demonstrated that endophytic microbes can promote plant growth and increase plant stress resistance. We aimed at isolating poplar endophytes able to increase their hosts' fitness both in nutrient-limited and polluted environments. To achieve this goal, endophytic bacteria and fungi were isolated from ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-017-1103-y
更新日期：2018-05-01 00:00:00
abstract：:The diversity and composition of soil bacterial communities in three topographic sites (summit, foot slope, and lakeshore) from subtropical montane forest ecosystem in Taiwan were examined by using 16S rRNA gene clone library analysis. This locality is temperate, perhumid, and has low soil acidity (pH < 4), which is a...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-009-9574-0
更新日期：2010-02-01 00:00:00
abstract：:A new membrane filter technique for field use was developed for the enumeration of either aerobic or anaerobic, autotrophic, sulfur-oxidizing bacteria in waters and soils. Immediately after collection, samples were filtered through sulfur-coated filters and incubated in selective media. Acidification or gas evolution ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02010741
更新日期：1977-12-01 00:00:00
abstract：:XTT (3'-[1-[(phenylamino)-carbonyl]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzenesulfonic acid hydrate), MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt), and WST-1 (4-(3-4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio)-1,3-benzenedisulfonate) are tetrazolium ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-004-0012-z
更新日期：2005-04-01 00:00:00
abstract：:The haptophyte Prymnesium parvum has lytic properties, and it affects coexisting phytoplankton species through allelopathy. We studied the effect of P. parvum allelochemicals on the lysis of the nontoxic and nonaxenic cryptomonad Rhodomonas salina and the consequent release of dissolved organic carbon (DOC). Changes i...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-006-9188-8
更新日期：2007-07-01 00:00:00
abstract：:With this study, we present first data on the diversity of aerobic methanotrophic bacteria (MOB) in an Arctic permafrost active layer soil of the Lena Delta, Siberia. Applying denaturing gradient gel electrophoresis and cloning of 16S ribosomal ribonucleic acid (rRNA) and pmoA gene fragments of active layer samples, w...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-008-9411-x
更新日期：2009-01-01 00:00:00
abstract：:Diverse microbial communities living in subsurface coal seams are responsible for important geochemical processes including the movement of carbon between the geosphere, biosphere and atmosphere. Microbial conversion of the organic matter in coal to methane involves a complex assemblage of bacteria and archaea working...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-019-01471-y
更新日期：2020-07-01 00:00:00
abstract：:The present study aimed to experimentally quantify the influence of a reduction of surface sediment permeability on microbial characteristics and ecological processes (respiration and leaf litter decomposition) occurring in the hyporheic zone (i.e. the sedimentary interface between surface water and groundwater). The ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-010-9774-7
更新日期：2011-05-01 00:00:00
abstract：:Soil contamination with heavy metals is a widespread problem, especially prominent on grounds lying in the vicinity of mines, smelters, and other industrial facilities. Many such areas are located in Southern Poland; they are polluted mainly with Pb, Zn, Cd, or Cu, and locally also with Cr. As for now, little is known...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-013-0344-7
更新日期：2014-04-01 00:00:00
abstract：:Heterotrophic bacteria are, in many aquatic systems, reliant on autochthonous organic carbon as their energy source. One exception is low-productive humic lakes, where allochthonous dissolved organic matter (ADOM) is the major driver. We hypothesized that bacterial production (BP) is similarly regulated in subarctic e...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-015-0714-4
更新日期：2016-05-01 00:00:00
abstract：:Quorum sensing (QS) signals have been considered to play important roles in biofilm development and in the attractiveness of biofilms to higher organisms in marine ecosystem. In this study, bacterial QS signalsacylated homoserine lactone derivatives (AHLs) were detected in 2-, 4-, and 6-day-old subtidal biofilms by us...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-007-9210-9
更新日期：2007-08-01 00:00:00
abstract：:Unicellular microalgae generally grow in the presence of bacteria, particularly when they are farmed massively. This study analyzes the bacteria associated with mass culture of Botryococcus braunii: both the planktonic bacteria in the water column and those forming biofilms adhered to the surface of the microalgal cel...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-010-9686-6
更新日期：2010-10-01 00:00:00
abstract：:Rice sheath rot has been mainly associated with the bacterial pathogen Pseudomonas fuscovaginae and in some cases to the fungal pathogen Sarocladium oryzae; it is yet unclear if they are part of a complex disease. The bacterial and fungal community associated with rice sheath rot symptomatic and asymptomatic rice plan...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-020-01529-2
更新日期：2020-10-01 00:00:00
abstract：:The dynamics of a marine virus-host system were investigated at different steady state growth rates in chemostat cultures and the data were analyzed using a simple model. The virus-host interactions showed strong dependence on host cell growth rate. The duration of the infection cycle and the virus burst size were fou...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s002480000050
更新日期：2000-08-01 00:00:00
abstract：:Fungi play an important role in cycling organic matter and nutrients in marine ecosystems. However, the distribution of fungal communities in the ocean, especially the vertical distribution along depth in the water column, remained poorly understood. Here, we assess the depth-related distribution pattern of fungal com...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-019-01374-y
更新日期：2019-11-01 00:00:00
abstract：:Bacterial respiration contributes to atmospheric carbon dioxide accumulation and development of hypoxia and is a critical, often overlooked, component of ecosystem function. This study investigates the concept that maintenance respiration is a significant proportion of bacterial respiration at natural nutrient levels ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-018-1244-7
更新日期：2019-04-01 00:00:00
abstract：:Persistence of recombinant and wild-type nucleopolyhedroviruses (NPV) was compared in field and laboratory microcosm experiments. Horizontal and vertical distribution of the viruses also was monitored in the field agricultural soil. Mixed populations of the bollworm, Helicoverpa zea, and tobacco budworm, Heliothis vir...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s002480000088
更新日期：2001-04-01 00:00:00
abstract：:Lichens host highly diverse microbial communities, with bacteria being one of the most explored groups in terms of their diversity and functioning. These bacteria could partly originate from symbiotic propagules developed by many lichens and, perhaps more commonly and depending on environmental conditions, from differ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-020-01662-y
更新日期：2021-01-06 00:00:00
abstract：:Bacteria attached to rock and glass surfaces were studied in streams draining a whole-tree harvested watershed (WTH) and a nonharvested (CONTROL) watershed in the Hubbard Brook Experimental Forest, New Hampshire, U.S.A. Seasonal trends in numbers of cells/cm(2), mean cell volume, cell size-frequency distribution, and ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02018912
更新日期：1988-09-01 00:00:00
abstract：:The ecological importance of microbial symbioses in terrestrial soils is widely recognized, but their role in soils that accumulate in forest canopies is almost entirely unknown. To address this gap, this study investigated the Frankia-Alnus rubra symbiosis in canopy and forest floor roots at Olympic National Park, WA...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-009-9587-8
更新日期：2010-02-01 00:00:00
abstract：:Rice root-associated microbial community play an important role in plant nutrient acquisition, biomass production, and stress tolerance. Herein, root-associated community assembly was investigated under different phosphate input levels in phosphorus (P)-deficient paddy soil. Rice was grown in a long-term P-depleted pa...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-019-01407-6
更新日期：2020-02-01 00:00:00
abstract：:To date, little is known about the diazotrophs in freshwater ecosystems. In this study, we examined the diversity, abundance, and distribution of the diazotrophic community in the deep oligotrophic Lake Fuxian using high-throughput sequencing and quantitative polymerase chain reaction of nifH genes. Our results showed...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-019-01461-0
更新日期：2020-05-01 00:00:00
abstract：:The occurrence and distribution of Azorhizobium and Rhizobium strains that induce stem nodulation of Sesbania rostrata were determined in four vegetation zones in Senegal. Based on tests with 16 Rhizobium and 10 Azorhizobium strains nodulating S. rostrata, a method was devised to distinguish among the strains. In all ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF00217424
更新日期：1995-01-01 00:00:00
abstract：:To reveal the variation of microbial community functions during water filtration process in river sediments, which has been utilized widely in natural water treatment systems, this study investigates the influence of municipal wastewater discharge to streams on the phylotype and metabolic potential of the microbiome i...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-015-0680-x
更新日期：2016-01-01 00:00:00
abstract：:The spatial distribution of the bacterial biomass and production and of potential heterotrophic activity (PHA) were measured every 4 h between 23 July (10:00 h) and 25 July (10:00 h) 1997 in a recently flooded oligo-mesotrophic reservoir (the Sep Reservoir, Puy-de-Dôme, France), in relation to temperature, the phytopl...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s002480000056
更新日期：2000-12-01 00:00:00
abstract：:Two areas of Chesapeake Bay, Colgate Creek in Baltimore Harbor and Eastern Bay, are presently under study, with routine sampling of water and sediment for petroleum-degrading microorganisms (bacteria, yeasts, and fungi) by direct plating and enrichment culture. Selected physical and chemical parameters are recorded fo...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02512380
更新日期：1974-12-01 00:00:00
abstract：:This study evaluated the effect of the antiretroviral ritonavir on protease secretion in different strains of Cryptococcus neoformans isolated from the environment and investigated the expression of heat shock protein (Hsp90), classically described virulence factors in other yeast in the presence of the same antiretro...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-016-0900-z
更新日期：2017-04-01 00:00:00
abstract：:Endophytes are microbial symbionts living inside plants and have been extensively researched in recent decades for their functions associated with plant responses to environmental stress. We conducted a meta-analysis of endophyte effects on host plants' growth and fitness in response to three abiotic stress factors: d...
journal_title：Microbial ecology
pub_type： 杂志文章,meta分析
doi：10.1007/s00248-017-1054-3
更新日期：2018-02-01 00:00:00
abstract：:A BSTRACTInterspecific fungal interactions are important ecological processes, whereas their physiological mechanisms are little understood. The aim of this work was to study how activity of fungal extracellular laccase was changed across mycelia during interactions between white- and brown-rot basidiomycetes from dif...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s002480000022
更新日期：2000-04-01 00:00:00
abstract：:The anaerobic degradation ofp-cresol was studied with one sediment source under three reducing conditions-denitrifying, sulfidogenic, and methanogenic. Loss ofp-cresol (1 mM) in all the anaerobic systems took initially 3 to 4 weeks. In acclimated culturesp-cresol was degraded in less than a week.p-Cresol was completel...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02543873
更新日期：1990-12-01 00:00:00
