Abstract:
:This study evaluated the effect of the antiretroviral ritonavir on protease secretion in different strains of Cryptococcus neoformans isolated from the environment and investigated the expression of heat shock protein (Hsp90), classically described virulence factors in other yeast in the presence of the same antiretroviral. The presence of the enzyme was detected by the formation of a degradation of the halo around the colonies. The results were classified as follows: level 1 (without proteases), level 2 (positive for proteases), and level 3 (strongly positive for proteases). Total protein extract isolated from the cell walls of the 12 strains incubated in the absence and presence of ritonavir (0.3125 mg mL-1) were resolved by SDS-PAGE and analyzed by Western blot assays using an antiserum against Hsp90 from Blastocladiella emersonii. All strains tested showed inhibition of proteinase activity in the presence of ritonavir at 0.3125 to 1.25 mg mL-1. High levels of Hsp90 were observed in the absence of ritonavir (0.3125 mg mL-1), except for the non-virulent control cells. In contrast, in the presence of the antiretroviral, a drastic reduction in the expression of the chaperone was observed. The data suggest that ritonavir, in addition to containing viral replication, could inhibit the expression of virulence factors in opportunistic yeast, as proteases and Hsp90. According to our current knowledge, this is the first time that the inhibition of Hsp90 by an antiretroviral was reported for environmental isolates of C. neoformans.
journal_name
Microb Ecoljournal_title
Microbial ecologyauthors
Serafin CF,Paris AP,Paula CR,Simão RC,Gandra RFdoi
10.1007/s00248-016-0900-zsubject
Has Abstractpub_date
2017-04-01 00:00:00pages
583-589issue
3eissn
0095-3628issn
1432-184Xpii
10.1007/s00248-016-0900-zjournal_volume
73pub_type
杂志文章abstract::Our goals were to evaluate the tolerance of mesorhizobia to acid and alkaline conditions as well as to investigate whether acid tolerance is related to the species or the origin site of the isolates. In addition, to investigate the molecular basis of acid tolerance, the expression of chaperone genes groEL and dnaKJ wa...
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journal_title:Microbial ecology
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doi:10.1007/s00248-008-9482-8
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pub_type: 杂志文章
doi:10.1007/s00248-010-9666-x
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journal_title:Microbial ecology
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journal_title:Microbial ecology
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journal_title:Microbial ecology
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doi:10.1007/s00248-017-1068-x
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journal_title:Microbial ecology
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journal_title:Microbial ecology
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