Detecting Associations Between Ciliated Protists and Prokaryotes with Culture-Independent Single-Cell Microbiomics: a Proof-of-Concept Study.

Abstract:

:Symbioses between prokaryotes and microbial eukaryotes, particularly ciliated protists, have been studied for a long time. Nevertheless, researchers have focused only on a few host genera and species, mainly due to difficulties in cultivating the hosts, and usually have considered a single symbiont at a time. Here, we present a pilot study using a single-cell microbiomic approach to circumvent these issues. Unicellular ciliate isolation followed by simultaneous amplification of eukaryotic and prokaryotic markers was used. Our preliminary test gave reliable and satisfactory results both on samples collected from different habitats (marine and freshwater) and on ciliates belonging to different taxonomic groups. Results suggest that, as already assessed for many macro-organisms like plants and metazoans, ciliated protists harbor distinct microbiomes. The applied approach detected new potential symbionts as well as new hosts for previously described ones, with relatively low time and cost effort and without culturing. When further developed, single-cell microbiomics for ciliates could be applied to a large number of studies aiming to unravel the evolutionary and ecological meaning of these symbiotic systems.

journal_name

Microb Ecol

journal_title

Microbial ecology

authors

Rossi A,Bellone A,Fokin SI,Boscaro V,Vannini C

doi

10.1007/s00248-018-1279-9

subject

Has Abstract

pub_date

2019-07-01 00:00:00

pages

232-242

issue

1

eissn

0095-3628

issn

1432-184X

pii

10.1007/s00248-018-1279-9

journal_volume

78

pub_type

杂志文章
  • Drag-reducing properties of bacteria from the skin mucus of the cornetfish (Fistularia commersonii).

    abstract::Scanning electron microscopy and fluorescence microscopy after scanning with DAPI indicated that the skin mucus of the cornetfish contained large numbers of bacteria, 4 × 10(8) per cm3. However, viable counts yielded only 2 × 104 per cm(3). Twelve bacterial strains were isolated directly from the mucus and another ten...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF00171971

    authors: Bernadsky G,Rosenberg E

    更新日期:1992-07-01 00:00:00

  • Litter Quality Modulates Effects of Dissolved Nitrogen on Leaf Decomposition by Stream Microbial Communities.

    abstract::Rates of leaf litter decomposition in streams are strongly influenced both by inorganic nutrients dissolved in stream water and by litter traits such as lignin, nitrogen (N) and phosphorus (P) concentrations. As a result, decomposition rates of different leaf species can show contrasting responses to stream nutrient e...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-019-01353-3

    authors: Jabiol J,Lecerf A,Lamothe S,Gessner MO,Chauvet E

    更新日期:2019-05-01 00:00:00

  • Structural and functional responses of a sewage microbial community to dilution-induced reductions in diversity.

    abstract::The relationship between functional redundancy and microbial community structure-diversity was examined using laboratory incubations to ensure constant environmental conditions. Serial dilutions of a sewage microbial community were prepared, used to inoculate sterile sewage, and maintained in batch culture. Probabilit...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-006-9033-0

    authors: Franklin RB,Mills AL

    更新日期:2006-08-01 00:00:00

  • Application of a novel rpoC1-RFLP approach reveals that marine Prochlorococcus populations in the Atlantic gyres are composed of greater microdiversity than previously described.

    abstract::To elucidate the degree of microdiversity within the genus Prochlorococcus, novel Prochlorococcus-specific polymerase chain reaction (PCR) primers were developed for the rpoC1 gene, which encodes the ribonucleic acid (RNA) polymerase core subunit. The size of the PCR fragment (925 bp) coupled with high sequence variat...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-007-9259-5

    authors: Jameson E,Joint I,Mann NH,Mühling M

    更新日期:2008-01-01 00:00:00

  • Killing of Escherichia coli by Myxococcus xanthus in aqueous environments requires exopolysaccharide-dependent physical contact.

    abstract::Nutrient or niche-based competition among bacteria is a widespread phenomenon in the natural environment. Such interspecies interactions are often mediated by secreted soluble factors and/or direct cell-cell contact. As ubiquitous soil bacteria, Myxococcus species are able to produce a variety of bioactive secondary m...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-013-0252-x

    authors: Pan H,He X,Lux R,Luan J,Shi W

    更新日期:2013-10-01 00:00:00

  • Biological soil crusts of sand dunes in Cape Cod National Seashore, Massachusetts, USA.

    abstract::Biological soil crusts cover hundreds of hectares of sand dunes at the northern tip of Cape Cod National Seashore (Massachusetts, USA). Although the presence of crusts in this habitat has long been recognized, neither the organisms nor their ecological roles have been described. In this study, we report on the microbi...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-004-0254-9

    authors: Smith SM,Abed RM,Gercia-Pichel F

    更新日期:2004-08-01 00:00:00

  • Antimicrobial resistance, virulence genes, and genetic lineages of Staphylococcus pseudintermedius in healthy dogs in tunisia.

    abstract::Nasal swabs of 100 healthy dogs were obtained in 2011 in Tunisia and tested for Staphylococcus pseudintermedius recovery. Antimicrobial resistance profile and virulence gene content were determined. Multilocus-sequence-typing (MLST) and SmaI-pulsed-field gel electrophoresis (PFGE) were investigated. S. pseudintermediu...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-013-0243-y

    authors: Gharsa H,Ben Slama K,Gómez-Sanz E,Lozano C,Klibi N,Jouini A,Messadi L,Boudabous A,Torres C

    更新日期:2013-08-01 00:00:00

  • Do Endophytes Promote Growth of Host Plants Under Stress? A Meta-Analysis on Plant Stress Mitigation by Endophytes.

    abstract::Endophytes are microbial symbionts living inside plants and have been extensively researched in recent decades for their functions associated with plant responses to environmental stress. We conducted a meta-analysis of endophyte effects on host plants' growth and fitness in response to three abiotic stress factors: d...

    journal_title:Microbial ecology

    pub_type: 杂志文章,meta分析

    doi:10.1007/s00248-017-1054-3

    authors: Rho H,Hsieh M,Kandel SL,Cantillo J,Doty SL,Kim SH

    更新日期:2018-02-01 00:00:00

  • Nutrient and temperature limitation of bacterioplankton growth in temperate lakes.

    abstract::Limitation of bacterioplankton production by nutrients and temperature was investigated in eight temperate lakes in summer. Six of the lakes were resampled in autumn. The lakes differ in nutrient content, water color, and concentration of dissolved organic carbon. Nutrients (phosphorus, nitrogen, and organic carbon) w...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-004-0259-4

    authors: Vrede K

    更新日期:2005-02-01 00:00:00

  • The establishment of an intestinal microflora in developing goldfish (Carassius auratus) of culture ponds.

    abstract::The bacterial flora in the intestinal tract of goldfish (Carassius auratus) was investigated at different stages of fish development. The floras of the diets and the water and sediment of a culture pond were also analyzed. The total counts in the intestine ranged from 2.2 × 10(6)-2.1 × 10(8) cells g(-1) wet weight.Aer...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02012646

    authors: Sugita H,Tsunohara M,Ohkoshi T,Deguchi Y

    更新日期:1988-05-01 00:00:00

  • Bacterial activity at the air/water interface.

    abstract::By using substrate molecules of varying degrees of surface activity, we were able to measure some features of bacterial activity in the surface microlayers (SM) and in the subsurface (bulk) water. The fraction of active cells was determined by a combined microautoradiography-epifluorescence (ME) method. Measurements w...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02019021

    authors: Hermansson M,Dahlbäck B

    更新日期:1983-12-01 00:00:00

  • Distribution of ultramicrobacteria in a gulf coast estuary and induction of ultramicrobacteria.

    abstract::The abundance of ultramicrobacteria (i.e., bacteria that pass through a 0.2μm filter) in a subtropical Alabama estuary was determined during a 1-year period. Although phenotypic and molecular characterization indicated that the population of ultramicrobacteria was dominated byVibrio species, species ofListonella andPs...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02013017

    authors: Hood MA,Macdonell MT

    更新日期:1987-09-01 00:00:00

  • Detection of Spiroplasma and Wolbachia in the bacterial gonad community of Chorthippus parallelus.

    abstract::We have recently detected the endosymbiont Wolbachia in multiple individuals and populations of the grasshopper Chorthippus parallelus (Orthoptera: acrididae). This bacterium induces reproductive anomalies, including cytoplasmic incompatibility. Such incompatibilities may help explain the maintenance of two distinct s...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-013-0226-z

    authors: Martínez-Rodríguez P,Hernández-Pérez M,Bella JL

    更新日期:2013-07-01 00:00:00

  • Molecular Mechanisms of Enhanced Bacterial Growth on Hexadecane with Red Clay.

    abstract::Red clay was previously used to enhance bioremediation of diesel-contaminated soil. It was speculated that the enhanced degradation of diesel was due to increased bacterial growth. In this study, we selected Acinetobacter oleivorans DR1, a soil-borne degrader of diesel and alkanes, as a model bacterium and performed t...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-015-0624-5

    authors: Jung J,Jang IA,Ahn S,Shin B,Kim J,Park C,Jee SC,Sung JS,Park W

    更新日期:2015-11-01 00:00:00

  • Conceptual bases for prey biorecognition and feeding selectivity in the microplanktonic marine phagotroph Oxyrrhis marina.

    abstract::It is suspected that phagotrophic marine protozoa might possess feeding receptors that enable them to discern the nutritional quality of individual prey items (during prey-handling) on the basis of their cell-surface biochemistry. This article reviews advances in our understanding of the molecular mechanisms that medi...

    journal_title:Microbial ecology

    pub_type: 杂志文章,评审

    doi:10.1007/s00248-008-9421-8

    authors: Martel CM

    更新日期:2009-05-01 00:00:00

  • The relevance of conditional dispersal for bacterial colony growth and biodegradation.

    abstract::Bacterial degradation is an ecosystem service that offers a promising method for the remediation of contaminated soils. To assess the dynamics and efficiency of bacterial degradation, reliable microbial simulation models, along with the relevant processes, are required. We present an approach aimed at improving reliab...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-011-9927-3

    authors: Banitz T,Johst K,Wick LY,Fetzer I,Harms H,Frank K

    更新日期:2012-02-01 00:00:00

  • A model for the density ofAeromonas hydrophila in Albemarle Sound, North Carolina.

    abstract::The abundance ofAeromonas hydrophila was measured monthly at 29 sites in Albemarle Sound, North Carolina and its tributaries from April 1977 through July 1979. Simultaneous measurements included heterotrophic plate count bacteria, fecal coliform bacteria, and 18 physical and chemical parameters. Using only 6 water qua...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02015127

    authors: Hazen TC

    更新日期:1983-07-01 00:00:00

  • A radioisotope assay for the quantification of hydrocarbon biodegradation potential in environmental samples.

    abstract::An enrichment culture method is described for quantifying the activity of hydrocarbon oxidizing bacteria in water and sediments. Application of the procedure indicated that the hydrocarbon oxidizing potential of environmental samples reflects the hydrocarbon burden of the area, the ability of the microflora to utilize...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02010379

    authors: Caparello DM,Larock PA

    更新日期:1975-03-01 00:00:00

  • The use of colony development for the characterization of bacterial communities in soil and on roots.

    abstract::A simple agar plating method for the description of microbial communities is described. This method is based on the quantification of the numbers of bacterial colonies in 6-7 age-based classes as they appear on agar media over a period of 6-10 days. The method can be used to quantify microbial communities in different...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF00170116

    authors: De Leij FA,Whipps JM,Lynch JM

    更新日期:1994-01-01 00:00:00

  • Colonization and Competition Dynamics of Plant Growth-Promoting/Inhibiting Bacteria in the Phytosphere of the Duckweed Lemna minor.

    abstract::Despite the considerable role of aquatic plant-associated bacteria in host plant growth and nutrient cycling in aquatic environments, the mode of their plant colonization has hardly been understood. This study examined the colonization and competition dynamics of a plant growth-promoting bacterium (PGPB) and two plant...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-018-1306-x

    authors: Ishizawa H,Kuroda M,Inoue K,Inoue D,Morikawa M,Ike M

    更新日期:2019-02-01 00:00:00

  • Influence of cation content on adenosine triphosphate determinations in soil.

    abstract::Adenosine triphosphate in soil is measured by the luciferin-luciferase bioluminescence method. Cation exchange in the ATP extraction procedure is used to reduce the content of, e.g., Fe, Al, and Cations in extracts. The efficiency of the cation exchange is determined by measuring the concentration of Fe by X-ray fluor...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02010504

    authors: Eiland F,Nielsen BS

    更新日期:1979-06-01 00:00:00

  • A study of the occurrence and distribution of bdellovibrios in estuarine sediment over an annual cycle.

    abstract::The recovery of bdellovibrios from estuarine sediments over an annual cycle was studied. Greater numbers of the predators were recovered in sediment than in the water column. Increases in the number of bdellovibrios recovered from sediment over various periods of time suggest that multiplication of the predators occur...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02012949

    authors: Williams HN

    更新日期:1988-01-01 00:00:00

  • Detrital floc and surface soil microbial biomarker responses to active management of the nutrient impacted Florida everglades.

    abstract::Alterations in microbial community composition, biomass, and function in the Florida Everglades impacted by cultural eutrophication reflect a new physicochemical environment associated with monotypic stands of Typha domingensis. Phospholipid fatty acid (PLFA) biomarkers were used to quantify microbial responses in det...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-012-0090-2

    authors: Bellinger BJ,Hagerthey SE,Newman S,Cook MI

    更新日期:2012-11-01 00:00:00

  • Rock phosphate solubilization and colonization of maize rhizosphere by wild and genetically modified strains of Penicillium rugulosum.

    abstract::Maize root colonization and phosphate solubilizing activity of the fungus Penicillium rugulosum were assessed in a greenhouse trial using soil-plant microcosms. The bacterial gene hph conferring resistance to hygromicin B was introduced by electroporation in the wild-type strain IR-94MF1 of P. rugulosum and one transf...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-002-1001-8

    authors: Reyes I,Bernier L,Antoun H

    更新日期:2002-07-01 00:00:00

  • Effect of freeze-thaw cycles on bacterial communities of arctic tundra soil.

    abstract::The effect of freeze-thaw (FT) cycles on Arctic tundra soil bacterial community was studied in laboratory microcosms. FT-induced changes to the bacterial community were followed over a 60-day period by terminal restriction fragment length polymorphism (T-RFLP) profiles of amplified 16S rRNA genes and reverse transcrib...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-009-9516-x

    authors: Männistö MK,Tiirola M,Häggblom MM

    更新日期:2009-10-01 00:00:00

  • Most acid-tolerant chickpea mesorhizobia show induction of major chaperone genes upon acid shock.

    abstract::Our goals were to evaluate the tolerance of mesorhizobia to acid and alkaline conditions as well as to investigate whether acid tolerance is related to the species or the origin site of the isolates. In addition, to investigate the molecular basis of acid tolerance, the expression of chaperone genes groEL and dnaKJ wa...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-012-0098-7

    authors: Brígido C,Oliveira S

    更新日期:2013-01-01 00:00:00

  • Measurement of Electron Transport System (ETS) activity in soil.

    abstract::Electron transport system (ETS) activity was measured in amended and nonamended soil by measuring the reduction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT) to iodonitrotetrazolium formazan (INT-formazan), which can be easily extracted with methanol without interference from other compound...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02010449

    authors: Trevors JT,Mayfield CI,Inniss WE

    更新日期:1982-10-01 00:00:00

  • Comparison of soil bacterial communities of Pinus patula of Nilgiris, western ghats with other biogeographically distant pine forest clone libraries.

    abstract::The bacterial community structure of the rhizosphere and non-rhizosphere soil of Pinus patula, found in the Nilgiris region of Western Ghats, was studied by constructing 16S rRNA gene clone libraries. In the rhizosphere and non-rhizosphere soil clone libraries constructed, 13 and 15 bacterial phyla were identified, re...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-012-0167-y

    authors: Rohini-Kumar M,Osborne JW,Saravanan VS

    更新日期:2013-07-01 00:00:00

  • Laboratory-induced endolithic growth in calcarenites: biodeteriorating potential assessment.

    abstract::This study is aimed to assess the formation of photosynthetic biofilms on and within different natural stone materials, and to analyse their biogeophysical and biogeochemical deterioration potential. This was performed by means of artificial colonisation under laboratory conditions during 3 months. Monitoring of micro...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-010-9666-x

    authors: Miller AZ,Rogerio-Candelera MA,Laiz L,Wierzchos J,Ascaso C,Sequeira Braga MA,Hernández-Mariné M,Maurício A,Dionísio A,Macedo MF,Saiz-Jimenez C

    更新日期:2010-07-01 00:00:00

  • Microhabitats and chemical microenvironments under saxicolous lichens growing on granite.

    abstract::Lasallia hispanica, Parmelia omphalodes, and Cornicularia normoerica, saxicolous thalli growing on granite, show a close relationship with other lichens and microorganisms living in the lithic substrate beneath them. The lithobiontic community is an accumulation of microorganisms at an interface forming a biofilm, whi...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-001-1028-2

    authors: de los Ríos A,Wierzchos J,Ascaso C

    更新日期:2002-01-01 00:00:00