Abstract:
:Abstract In this study, two different agricultural soils were investigated: one organic soil and one sandy soil, from Stend (south of Bergen), Norway. The sandy soil was a field frequently tilled and subjected to crop rotations. The organic soil was permanent grazing land, infrequently tilled. Our objective was to compare the diversity of the cultivable bacteria with the diversity of the total bacterial population in soil. About 200 bacteria, randomly isolated by standard procedures, were investigated. The diversity of the cultivable bacteria was described at phenotypic, phylogenetic, and genetic levels by applying phenotypical testing (Biolog) and molecular methods, such as amplified rDNA restriction analysis (ARDRA); hybridization to oligonucleotide probes; and REP-PCR. The total bacterial diversity was determined by reassociation analysis of DNA isolated from the bacterial fraction of environmental samples, combined with ARDRA and DGGE analysis. The relationship between the diversity of cultivated bacteria and the total bacteria was elucidated. Organic soil exhibited a higher diversity for all analyses performed than the sandy soil. Analysis of cultivable bacteria resulted in different resolution levels and revealed a high biodiversity within the population of cultured isolates. The difference between the two agricultural soils was significantly higher when the total bacterial population was analyzed than when the cultivable population was. Thus, analysis of microbial diversity must ultimately embrace the entire microbial community DNA, rather than DNA from cultivable bacteria.
journal_name
Microb Ecoljournal_title
Microbial ecologyauthors
Øvreås L,Torsvik V Vdoi
10.1007/s002489900117subject
Has Abstractpub_date
1998-11-01 00:00:00pages
303-315issue
3eissn
0095-3628issn
1432-184Xpii
MECOJKF98-14journal_volume
36pub_type
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journal_title:Microbial ecology
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doi:10.1007/s00248-011-9970-0
更新日期:2012-04-01 00:00:00
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journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02020340
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journal_title:Microbial ecology
pub_type: 杂志文章
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更新日期:2004-11-01 00:00:00
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journal_title:Microbial ecology
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更新日期:2006-08-01 00:00:00
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journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02543883
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journal_title:Microbial ecology
pub_type: 杂志文章
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