Metabolic profiling of dialysate at sensitized acupoints in knee osteoarthritis patients: A study protocol.

Abstract:

BACKGROUND:Acupuncture therapy is frequently used to treat Knee Osteoarthritis (KOA) in clinic, and usually used local acupoints near the diseased knees as therapeutic targets. Some local acupoints appeared sensitization phenomenon which was called sensitized acupoints, which were regarded as important therapeutic targets to get better therapeutic effect on clinic. Therefore, it is necessary to explore the biological basis of acupoint sensitization. Meanwhile, there is a lack of an analysis of the metabolism for sensitized acupoints in KOA patients. Considering that acupuncture effect could be multi-targeted, omics (such as metabolomics) may be a useful method to reveal the relationship between sensitized acupoints and clinical efficacy of acupuncture. METHODS AND ANALYSIS:This study is a parallel design trial. Thirty KOA patients and 30 healthy volunteers will be recruited in this study. Mechanical pain threshold will be measured by Electron Von frey in order to confirm the highest sensitized acupoints. Then collect tissue fluid from the highest sensitized acupoints by micro dialysis technical, then apply electro-acupuncture method on the highest sensitized acupoints to treat KOA patients, after 20 sessions treatments, measure and collect again. Liquid chromatography-tandem mass spectrometry method will be used to analyze the metabonomics of dialysate. RESULTS:This study will provide a high-quality evidence to reveal the local molecular mechanism of acupuncture sensitized acupoints for patient with KOA. CONCLUSION:This study will provide up-date evidence of whether acupuncture sensitized acupoints have local molecular mechanism for KOA. TRIAL REGISTRATION NUMBER:NCT03599180 (24 Jul. 2018).

journal_name

Medicine (Baltimore)

journal_title

Medicine

authors

Li S,Chai XN,Zuo CY,Lv P,Tang Y,Tan HJ,Liu LZ,Yin HY,Yu SG

doi

10.1097/MD.0000000000017843

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

e17843

issue

45

eissn

0025-7974

issn

1536-5964

pii

00005792-201911080-00034

journal_volume

98

pub_type

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