Proteoglycans from cultures of fibroblast from the human uterine cervix.

Abstract:

:A small dermatan sulphate proteoglycan has been isolated from the human uterine cervix. The concentration of this proteoglycan has been shown to decrease during pregnancy. To study the mechanism behind this change fibroblasts from the human uterine cervix were established in culture. Their synthesis and secretion of proteoglycans were investigated by incubating the monolayer with 35SO4 and (3H) leucine for 48 h. Two dermatan sulphate proteoglycans and one heparan sulphate proteoglycan were isolated from the medium. The cell layer contained the same proteoglycans although in different proportions. The major dermatan sulphate proteoglycan accounting for 55% of the total macromolecular 35S was small with a molecular weight (MW) of 100,000 daltons. The side chains were calculated to be approximately 1-3 per protein core and included more than 50% iduronic acid containing disaccharides. This small dermatan sulphate proteoglycan is very similar to that isolated from the intact human uterine cervix. However, when cells were established in culture an additional larger dermatan sulphate proteoglycan with an MW of 400,000 daltons was synthesized. This proteoglycan was substituted for 4-8 polysaccharide side chains rich in glucuronic acid. 24% of the total macromolecular 35S were found in this proteoglycan.

journal_name

Gynecol Obstet Invest

authors

Uldbjerg N,Malmström A,Ekman G,Ulmsten U

doi

10.1159/000299026

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

146-54

issue

3

eissn

0378-7346

issn

1423-002X

journal_volume

19

pub_type

杂志文章
  • Immunoradiometric studies with a monoclonal antibody against an estrogen-receptor-related protein in human breast cancer.

    abstract::The concentration of P29, a phosphoprotein related to estradiol receptor (RE), was measured by an immunoradiometric assay in human breast cancer cytosol and the results compared with the levels of RE and progesterone receptors (RP), as measured by the classical ligand-binding assay. Good linear correlation was found b...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000293689

    authors: Cano A,Rivera J,Parrilla JJ,Abad L

    更新日期:1988-01-01 00:00:00

  • Effectiveness and Adverse Events of Early Laparoscopic Therapy versus Conservative Treatment for Tubo-Ovarian or Pelvic Abscess: A Single-Center Retrospective Cohort Study.

    abstract:BACKGROUND/AIM:We aimed to assess the value of early laparoscopic therapy in management of tubo-ovarian abscess (TOA) or pelvic abscess. METHODS:This was a retrospective study of all consecutive patients who were initially diagnosed with TOA or pelvic abscess at the local hospital between January 2010 and December 201...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000493855

    authors: Chu L,Ma H,Liang J,Li L,Shen A,Wang J,Li H,Tong X

    更新日期:2019-01-01 00:00:00

  • Gestational diabetes: an evaluation of serum fructosamine as a screening test in a high-risk population.

    abstract:AIM:To evaluate the value of serum fructosamine as a screening test for gestational diabetes mellitus (GDM). METHODS:849 pregnant women underwent the one-step 75 g oral glucose tolerance test (OGTT) for universal screening of GDM. The fasting serum fructosamine (cFruc) was assessed using the area under the receiver op...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000318705

    authors: Agarwal MM,Dhatt GS,Othman Y,Ljubisavljevic MR

    更新日期:2011-01-01 00:00:00

  • Relationship between recurrent miscarriage and insulin resistance.

    abstract:AIMS:To investigate the differences in insulin resistance between women with recurrent miscarriage and those with normal pregnancy. METHODS:Pregnant women with a history of recurrent miscarriage were included in the patient group (n = 97), while those with no history of abnormal pregnancy were included in the control ...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000325165

    authors: Wang Y,Zhao H,Li Y,Zhang J,Tan J,Liu Y

    更新日期:2011-01-01 00:00:00

  • In vitro fertilization in Kock's ileostomy patients. Prior evaluation by pelvic ultrasonography.

    abstract::The study describes Kock's continent ileostomy in 2 females, both of whom were suffering from tubal infertility. Appropriate placement of the abdominal pouch, permitting in vitro fertilization embryo transfer treatment, was present in 1 patient. In the other patient, an ultrasonographic examination demonstrated the po...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000293324

    authors: Dor J,Shalev J,Shoham Z,Rabau M,Rudak E,Serr DM,Mashiach S

    更新日期:1990-01-01 00:00:00

  • Evaluation of the possible interaction of the antifungal triazole SCH 39304 with oral contraceptives in normal healthy women.

    abstract::The possible interaction of the newly developed triazole antifungal SCH 39304 with low-dose combined oral contraceptives (OCs) was studied in 15 healthy women. Serum levels of ovarian and adrenocortical steroids and sex hormone-binding globulin (SHBG) were analyzed in samples collected during the OC cycles immediately...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000293003

    authors: Lunell NO,Pschera H,Zador G,Carlström K

    更新日期:1991-01-01 00:00:00

  • Electro-oophorogram: A preliminary study of the electric activity of the ovary.

    abstract::The electric activity of the ovary was studied in 13 female mongrel dogs aiming at characterizing a normal electro-oophorogram (EOOG). Two monopolar silver-silver chloride electrodes were sutured under anesthesia to the ovary of the bitches which were in the anestrus phase of the estrous cycle. A daily recording sessi...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000010012

    authors: Shafik A

    更新日期:1998-08-01 00:00:00

  • The shape and dimensions of the human vagina as seen in three-dimensional vinyl polysiloxane casts.

    abstract::Full vaginal casts of 39 caucasian women, 13 each nulliparous, uniparous and multiparous, were made using vinyl polysiloxane impression material. Vaginal lengths were measured with polished acrylic rods inserted by the subjects, and introital diameters were measured with conical polypropylene flasks. Parallel sided, c...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000291946

    authors: Pendergrass PB,Reeves CA,Belovicz MW,Molter DJ,White JH

    更新日期:1996-01-01 00:00:00

  • Pregnancy rate after homologous insemination of pathological ejaculate both with and without the addition of kallikrein.

    abstract::As part of a gynecological-andrological cooperation programme for the treatment of infertile marriages made by the 1st Department of Obstetrics and Gynecology and the Department of Urology, University of Vienna Medical School, the pregnancy rates achieved through 1,269 homologous inseminations of pathological ejaculat...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000299429

    authors: Maier U,Grünberger W,Binstorfer E,Lunglmayr G

    更新日期:1983-01-01 00:00:00

  • Prostacyclin does not influence placental blood pool in vivo.

    abstract::In 20 healthy pregnant women placental blood pool was estimated by means of placental scintigrams before, during and after infusion of either prostacyclin (PGI2) or placebo for 10 min to study the effect of PGI2 on the local regulation of uterine blood flow. There was no difference in the mean number of counts between...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 临床试验,杂志文章

    doi:10.1159/000299012

    authors: Husslein P,Gitsch E,Pateisky N,Philipp K,Leodolter S,Sinzinger H

    更新日期:1985-01-01 00:00:00

  • Role of T-cell cytokines in decidua and in cumulus oophorus during pregnancy.

    abstract::We focus on the roles of decidual and cumulus oophorus T cells. It was suggested that Th1-type cytokines (IFN-gamma and TNF-beta), which promote allograft rejection, may compromise pregnancy, whereas the Th2-type cytokines (IL-4, IL-5, IL-10) inhibiting the Th1 responses promote allograft tolerance and therefore may i...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章,评审

    doi:10.1159/000101738

    authors: Piccinni MP

    更新日期:2007-01-01 00:00:00

  • Hydranencephaly with renal dysgenesis: a coincidental finding? Case report with review of the literature.

    abstract::We present the case of a female fetus aborted in the 20th week of gestation due to severely dysplastic kidneys, anhydramnios and hydranencephalus. The combination of these malformations is extremely rare, resulting in only 4 cases described so far. Our case is the first ever presented in a female showing polycystic-dy...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章,评审

    doi:10.1159/000291522

    authors: Gschwendtner A,Mairinger T,Soelder E,Alge A,Kreczy A

    更新日期:1997-01-01 00:00:00

  • Perinatal environment and endometriosis.

    abstract:BACKGROUND/AIMS:Perinatal environmental exposure may affect fetal development and reprogram the developing organism for adult-onset disease. In this case-control study, we aimed at assessing this pathogenetic model in endometriosis. METHODS:Consecutive patients with a first laparoscopic diagnosis of endometriosis were...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000323531

    authors: Somigliana E,Vigano P,Abbiati A,Paffoni A,Benaglia L,Vercellini P,Fedele L

    更新日期:2011-01-01 00:00:00

  • Can Genomic Amplification of Human Telomerase Gene and C-MYC in Liquid-Based Cytological Specimens Be Used as a Method for Opportunistic Cervical Cancer Screening?

    abstract:OBJECTIVE:To evaluate the effectiveness of five methods including the ThinPrep cytological test (TCT), liquid-based cytology, the human papillomavirus (HPV) test, detection of the TERC and C-MYC genes and visual inspection with acetic acid/Lugol's iodine (VIA/VILI) for opportunistic cervical cancer screening, and to ex...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000371760

    authors: Gao K,Eurasian M,Zhang J,Wei Y,Zheng Q,Ye H,Li L

    更新日期:2015-01-01 00:00:00

  • Fetal circulatory responses to maternal blood loss.

    abstract::We examined the fetal circulatory responses to maternal blood loss in pregnant women during the third trimester. Seven healthy women with placenta previa and singleton pregnancies underwent phlebotomies in an autologous donation program. Four hundred milliliters of blood was collected within 15 min at 34 and 35 weeks ...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000052915

    authors: Suzuki S,Tateoka S,Yagi S,Ishikawa G,Ohshita T,Sawa R,Yoneyama Y,Asakura H,Araki T

    更新日期:2001-01-01 00:00:00

  • Age distribution of erythrocyte population in women with twin pregnancy.

    abstract::An evaluation of the age distribution of erythrocytes in twin gestation was performed. Based on previous studies on age distribution of erythrocytes in late pregnancy, we have proposed that a more pronounced shift in age distribution of erythrocytes in twin pregnancy may be expected. We have used the density distribut...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000292618

    authors: Lurie S,Blickstein I

    更新日期:1993-01-01 00:00:00

  • A diminished intrapartum amniotic fluid index is a predictive marker of possible adverse neonatal outcome when associated with prolonged labor.

    abstract:OBJECTIVE:To determine whether a diminished intrapartum amniotic fluid volume represents a risk of adverse neonatal outcome when it occurs in conjunction with prolonged labor. METHODS:The intrapartum amniotic fluid index (iAFI) was measured in 242 parturients over 35 weeks of gestation during 1st-stage labor, and the ...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000049402

    authors: Kawasaki N,Nishimura H,Yoshimura T,Okamura H

    更新日期:2002-01-01 00:00:00

  • Genomewide oligonucleotide microarray analysis on placentae of pre-eclamptic pregnancies.

    abstract:OBJECTIVE:Human placentae from normal and pre-eclamptic pregnancies were evaluated for possible changes in gene expression by microarray analysis to uncover new clues for the research of the etiology of pre-eclampsia. METHODS:Placentae from five normal pregnancies and five pregnancies complicated by pre-eclampsia were...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000092857

    authors: Zhou R,Zhu Q,Wang Y,Ren Y,Zhang L,Zhou Y

    更新日期:2006-01-01 00:00:00

  • Hysterectomy and lower urinary tract symptoms: a nonrandomized comparison of vaginal and abdominal hysterectomy.

    abstract:BACKGROUND/AIMS:It has been reported that lower urinary tract symptoms (LUTS) are more prevalent in patients who have undergone hysterectomy. However, the effects of surgical approach of hysterectomy on micturition have not been well documented. The aim of this study is to compare LUTS between patients who underwent va...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章,多中心研究

    doi:10.1159/000297507

    authors: Lakeman MM,van der Vaart CH,Roovers JP,HysVA study group.

    更新日期:2010-01-01 00:00:00

  • Imbalance between lipid peroxidation and antioxidant status in preeclampsia.

    abstract::Lipid peroxidation and the antioxidant system were investigated in the plasma and placenta of normal and preeclamptic pregnant women. A significant increase in thiobarbituric acid reactive substance (TBARS), significant decreases in total thiol (t-SH) content and superoxide dismutase (SOD) activity, and unchanged vita...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000009994

    authors: Mutlu-Türkoglu U,Ademoglu E,Ibrahimoglu L,Aykaç-Toker G,Uysal M

    更新日期:1998-01-01 00:00:00

  • The Endometriosis Fertility Index Is Useful for Predicting the Ability to Conceive without Assisted Reproductive Technology Treatment after Laparoscopic Surgery, Regardless of Endometriosis.

    abstract:OBJECTIVE:The endometriosis fertility index (EFI) is a novel index for predicting pregnancy after surgery. We investigated the utility of the EFI for predicting the ability to conceive without assisted reproductive technology (ART) treatment after laparoscopic surgery. METHODS:From July 2011 through December 2012, we ...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000480454

    authors: Hobo R,Nakagawa K,Usui C,Sugiyama R,Ino N,Motoyama H,Kuribayashi Y,Inoue M,Sugiyama R

    更新日期:2018-01-01 00:00:00

  • Successful live births after surgical treatments for symptomatic cesarean scar pregnancies: report of 3 cases.

    abstract::There is no current consensus on the best treatment modality for cesarean scar pregnancy (CSP) with favorable reproductive and pregnancy outcome. We treated 3 cases of symptomatic CSP with fetal cardiac activity. The first case underwent laparoscopic repair at 6 weeks' gestational age of unruptured CSP. The second pat...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000364867

    authors: Yang G,Lee D,Lee S,Jeong K

    更新日期:2014-01-01 00:00:00

  • Ploidy analysis and S-phase fraction determination by flow cytometry in anembryonic pregnancy and spontaneous abortions.

    abstract::The nuclear DNA content of 20 anembryonic pregnancies was studied by flow cytometry from paraffin embedded tissue blocks. An abnormal amount of DNA content was found in 8 of the cases. This was a significantly higher percentage than encountered in spontaneous abortions studied by the same population (40 and 9%, respec...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 临床试验,杂志文章

    doi:10.1159/000010149

    authors: Ozeren M,Aydemir V,Tekelioglu Y,Topçuoglu K,Bozkaya H

    更新日期:1999-01-01 00:00:00

  • Lymphokine-activated killer activity in women with endometriosis.

    abstract::The aim of the present study was to investigate whether women with endometriosis displayed a decreased lymphokine-activated killer (LAK) activity. In 15 women with and 7 women without endometriosis the cytotoxicity against four different tumor cell lines--K562, the endometrium carcinomas AN3CA and RL95, the natural-ki...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000292556

    authors: Oosterlynck DJ,Lacquet FA,Waer M,Koninckx PR

    更新日期:1994-01-01 00:00:00

  • Laparoendoscopic single-site surgery for benign ovarian cystectomies.

    abstract:BACKGROUND:Single-port laparoscopy (LESS) utilizes a single, multichannel port in an attempt to decrease postoperative pain, while enhancing cosmesis and minimizing the potential risks and morbidities associated with the multiple ports used in conventional laparoscopy. METHODS:We performed a retrospective study examin...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000367659

    authors: Bedaiwy MA,Sheyn D,Eghdami L,Abdelhafez FF,Volsky JG,Fader AN,Escobar PF

    更新日期:2015-01-01 00:00:00

  • Polycystic Ovary Syndrome and Increased Soluble Tumor Necrosis Factor Like Weak Inducer of Apoptosis Levels Are Independent Predictors of Dyslipidemia in Youth.

    abstract:BACKGROUND/AIMS:Dyslipidemia is common in women with polycystic ovary syndrome (PCOS) irrespective of age. Our aim was to investigate soluble tumor necrosis factor like weak inducer of apoptosis (sTWEAK), a cardiovascular risk marker in PCOS, and to determine if it is associated with dyslipidemia in youth. METHODS:A p...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000447590

    authors: Erkenekli K,Oztas E,Kuscu E,Keskin U,Kurt YG,Tas A,Yilmaz N

    更新日期:2017-01-01 00:00:00

  • Is sentinel lymph node dissection an appropriate standard of care for low-stage endometrial cancers? A review of the literature.

    abstract:BACKGROUND/AIMS:To review the literature and compare detection rates, false negative (FN) rates, and negative predictive values (NPVs) of sentinel lymph node (SLN) biopsy in endometrial cancer to those in breast and vulvar cancer. Secondary objectives were to evaluate techniques of SLN biopsy in endometrial cancer. ME...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章,评审

    doi:10.1159/000353897

    authors: Levinson KL,Escobar PF

    更新日期:2013-01-01 00:00:00

  • Clinical evaluation of the Q-tip test in normal pregnant women.

    abstract::The crisis frequency of stress incontinence (SI) is known to be high among pregnant women. This preliminary study was performed to determine whether the diagnostic standard of the Q-tip test used in the diagnosis of SI in nonpregnant women is applicable to pregnant women. The Q-tip tests were performed regularly durin...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 临床试验,杂志文章

    doi:10.1159/000052900

    authors: Nakamura T,Ishiko O,Hirai K,Sumi T,Nakagawa E,Nakai Y,Tomoda S,Ogita S

    更新日期:2001-01-01 00:00:00

  • Association between adiponectin receptor 1 gene polymorphism and insulin resistance in Chinese patients with polycystic ovary syndrome.

    abstract:BACKGROUND/AIMS:Adiponectin receptor 1 (ADIPOR1) is an identified receptor for adiponectin, an adipocytokine with anti-inflammatory and insulin-sensitizing properties. The ADIPOR1 gene is a potential candidate gene in polycystic ovary syndrome (PCOS). The aim of this study is to assess the association between single-nu...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000356960

    authors: Yang Z,Yang X,Xu J,Sun Y,Shi Y,Fang S

    更新日期:2014-01-01 00:00:00

  • Metastatic choriocarcinoma presenting in a gravid woman.

    abstract::Choriocarcinoma coexistent with an intrauterine pregnancy is rare and requires decisions affecting mother and child to treat this aggressive tumor. An instance of this situation is presented to highlight clinical circumstances that warrant departure from accepted dogma. Pathologic aspects of choriocarcinoma are also d...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000363234

    authors: Byrd K,Seay R,Bechert CJ,Maxwell GL,Bicher A

    更新日期:2015-01-01 00:00:00