Abstract:
:The cytoskeleton provides structural integrity to cells and serves as a key component in mechanotransduction. Tensins are thought to provide a force-bearing linkage between integrins and the actin cytoskeleton; yet, direct evidence of tensin's role in mechanotransduction is lacking. We here report that local force application to epithelial cells using a micrometer-sized needle leads to rapid accumulation of cten (tensin 4), but not tensin 1, along a fibrous intracellular network. Surprisingly, cten-positive fibers are not actin fibers; instead, these fibers are keratin intermediate filaments. The dissociation of cten from tension-free keratin fibers depends on the duration of cell stretch, demonstrating that the external force favors maturation of cten-keratin network interactions over time and that keratin fibers retain remarkable structural memory of a cell's force-bearing state. These results establish the keratin network as an integral part of force-sensing elements recruiting distinct proteins like cten and suggest the existence of a mechanotransduction pathway via keratin network.
journal_name
Proc Natl Acad Sci U S Aauthors
Cheah JS,Jacobs KA,Heinrich V,Lo SH,Yamada Sdoi
10.1073/pnas.1911865116subject
Has Abstractpub_date
2019-10-01 00:00:00pages
19799-19801issue
40eissn
0027-8424issn
1091-6490pii
1911865116journal_volume
116pub_type
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