Abstract：
:An early bottleneck in the rapid isolation of new antibody fragment binders using in vitro library approaches is the inertia encountered in acquiring and preparing soluble antigen fragments. In this report, we describe a simple, yet powerful strategy that exploits the properties of the SpyCatcher/SpyTag (SpyC/SpyT) covalent interaction to improve substantially the speed and efficiency in obtaining functional antibody clones of interest. We demonstrate that SpyC has broad utility as a protein-fusion tag partner in a eukaryotic expression/secretion context, retaining its functionality and permitting the direct, selective capture and immobilization of soluble antigen fusions using solid phase media coated with a synthetic modified SpyT peptide reagent. In addition, we show that the expressed SpyC-antigen format is highly compatible with downstream antibody phage display selection and screening procedures, requiring minimal post-expression handling with no sample modifications. To illustrate the potential of the approach, we have isolated several fully human germline scFvs that selectively recognize therapeutically relevant native cell surface tumor antigens in various in vitro cell-based assay contexts.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Fierle JK,Abram-Saliba J,Brioschi M,deTiani M,Coukos G,Dunn SMdoi
10.1038/s41598-019-49233-7subject
Has Abstractpub_date
2019-09-06 00:00:00pages
12815issue
1issn
2045-2322pii
10.1038/s41598-019-49233-7journal_volume
9pub_type
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