Abstract:
:Articular chondrocytes are slowly dividing cells that tend to lose their cell type-specific phenotype and ability to produce structurally and functionally correct cartilage tissue when cultured. Thus, culture conditions, which enhance the maintenance of chondrocyte phenotype would be very useful for cartilage research. Here we show that Rho-kinase inhibition by Y-27632 under hypoxic conditions efficiently maintains and even enhances chondrocyte-specific extracellular matrix production by chondrocytic cells. The effects of long-term Y-27632 exposure to human chondrosarcoma 2/8 cell phenotype maintenance and extracellular matrix production were studied at normoxia and at a 5% low oxygen atmosphere. Y-27632 treatment at normoxia induced ACAN and COL2A1 gene up-regulation and a minor increase of sulfated glycosaminoglycans (sGAGs), while type II collagen expression was not significantly up-regulated. A further increase in expression of ACAN and COL2A1 was achieved with Y-27632 treatment and hypoxia. The production of sGAGs increased by 65.8%, and ELISA analysis revealed a 6-fold up-regulation of type II collagen. Y-27632 also induced the up-regulation of S100-A1 and S100-B proteins and modified the expression of several other S100 protein family members, such as S100-A4, S100-A6, S100-A13 and S100-A16. The up-regulation of S100-A1 and S100-B proteins is suggested to enhance the chondrocytic phenotype of these cells.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Piltti J,Bygdell J,Fernández-Echevarría C,Marcellino D,Lammi MJdoi
10.1038/s41598-017-03958-5subject
Has Abstractpub_date
2017-06-16 00:00:00pages
3708issue
1issn
2045-2322pii
10.1038/s41598-017-03958-5journal_volume
7pub_type
杂志文章abstract::TLR4 is implicated in diseases associated with chronic low-grade inflammation, yet homeostatic signaling mechanisms that prevent and/or are affected by chronic TLR4 activation are largely uncharacterized. We recently reported that LPS/TLR4 activates in human leukocytes signaling intermediates (SI), abbreviated TLR4-SI...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43347-8
更新日期:2019-05-07 00:00:00
abstract::We report a three dimensional (3D) quantitative visualization of a mammalian mitochondrion by coherent x-ray diffractive imaging (CXDI) using synchrotron radiation. The internal structures of a mitochondrion from a mouse embryonic fibroblast cell line (NIH3T3) were visualized by tomographic imaging at approximately 60...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01833-x
更新日期:2017-05-12 00:00:00
abstract::It has been postulated that there is a link between age related degenerative diseases and cancer. The TNF-related apoptosis-inducing ligand (TRAIL) has been shown to selectively kill tumor cells by binding to pro-apoptotic and anti-apoptotic receptors. Our aim was to study the levels of anti-apoptotic receptor (DcR1) ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04114
更新日期:2014-02-18 00:00:00
abstract::Mayaro virus (MAYV) causes an acute febrile illness similar to that produced by chikungunya virus (CHIKV), an evolutionary relative in the Semliki Forest virus complex of alphaviruses. MAYV emergence is typically sporadic, but recent isolations and outbreaks indicate that the virus remains a public health concern. Giv...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56551-3
更新日期:2019-12-31 00:00:00
abstract::Studies on the evolution of parental care have focused primarily on the costs and benefits of parental care and the life-history attributes that favour it. However, once care evolves, offspring in some taxa appear to become increasingly dependent on their parents. Although offspring dependency is a central theme in fa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29323
更新日期:2016-07-05 00:00:00
abstract::Polymer derived ceramic (PDC) composite coatings were deposited on AISI 304 substrates using siloxane based preceramic polymer polymethlysilsquioxane (PMS) and ZrSi2 as active filler or Ag as passive filler. The tribological performance of the composite coatings was evaluated at room temperature and moderately high te...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33441-8
更新日期:2018-10-10 00:00:00
abstract::Globally, lung cancer accounts for approximately 20% of all cancer related deaths. Five-year survival is poor and rates have remained unchanged for the past four decades. There is an urgent need to identify markers of lung carcinogenesis and new targets for therapy. Given the recent successes of immune modulators in c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18907
更新日期:2016-01-13 00:00:00
abstract::Organotypic, three-dimensional (3D) cancer models have enabled investigations of complex microtissues in increasingly realistic conditions. However, a drawback of these advanced models remains the poor biological relevance of cancer cell lines, while higher clinical significance would be obtainable with patient-derive...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06544-x
更新日期:2017-07-26 00:00:00
abstract::Chronic lymphocytic leukaemia (CLL) is characterised by a heterogeneous clinical course. Such heterogeneity is associated with a number of markers, including TP53 gene inactivation. While TP53 gene alterations determine resistance to chemotherapy, it is not clear whether they can influence early disease progression. T...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75364-3
更新日期:2020-10-28 00:00:00
abstract::Magnetotactic bacteria biomineralize ordered chains of uniform, membrane-bound magnetite or greigite nanocrystals that exhibit nearly perfect crystal structures and species-specific morphologies. Transmission electron microscopy (TEM) is a critical technique for providing information regarding the organization of cell...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06854
更新日期:2014-10-31 00:00:00
abstract::Ultrasounds (US) has been used in the past decades as a non-invasive imaging modality. Although employed extensively in clinical applications for soft tissue imaging, the acoustic beams can also be used for sensing and actuation for biological implants. In this paper we present a unified three dimensional (3D) computa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63097-2
更新日期:2020-04-16 00:00:00
abstract::Cell penetrating peptides (CPPs) are peptides that can be translocated into cells and used as a carrier platform for the intracellular uptake of cargo molecules. Subject to the source of CPP sequences and their positively charged nature, the cytotoxicity and immunogenicity of conventional CPPs needs to be optimized to...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11719
更新日期:2015-06-26 00:00:00
abstract::We aimed to determine the clinical and microbiological characteristics of Candida bloodstream infections in children and the impact of therapeutic strategies on outcomes. All pediatric patients with candidemia from a medical center in Taiwan over a 13-year period (2003-2015) were included and a total of 262 patients w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01123-6
更新日期:2017-04-24 00:00:00
abstract::Hurricanes are a persistent socio-economic hazard for countries situated in and around the Main Development Region (MDR) of Atlantic tropical cyclones. Climate-model simulations have attributed their interdecadal variability to changes in solar and volcanic activity, Saharan dust flux, anthropogenic greenhouse gas and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12838
更新日期:2015-08-05 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-24031-9
更新日期:2018-04-11 00:00:00
abstract::The growth kinetics of polymer thin films prepared by plasma-based deposition method were explored using atomic force microscopy. The growth behavior of the first layer of the polythiophene somewhat differs from that of the other layers because the first layer is directly deposited on the substrate, whereas the other ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11201
更新日期:2015-06-18 00:00:00
abstract::Cardiorenal syndrome is defined by primary heart failure conditions influencing or leading to renal injury or dysfunction. Dilated cardiomyopathy (DCM) is a major co-existing form of heart failure (HF) with renal diseases. Myocardin (MYOCD), a cardiac-specific co-activator of serum response factor (SRF), is increased ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42009-z
更新日期:2019-04-10 00:00:00
abstract::Aneuploidy has been reported to occur at remarkably high levels in normal somatic tissues using Fluorescence In Situ Hybridization (FISH). Recently, these reports were contradicted by single-cell low-coverage whole genome sequencing (scL-WGS) analyses, which showed aneuploidy frequencies at least an order of magnitude...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46606-w
更新日期:2019-07-19 00:00:00
abstract::The current study analyzed and optimized the concentration of NaOH for alkaline pretreatment of kitchen refuse for biogas production. Also, the benefits of microwave assistance in enhanced biogasification of kitchen refuse were evaluated. The TS, VS and structural changes were compared using standard experimental tech...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42471-9
更新日期:2019-04-12 00:00:00
abstract::There is an urgent need to quantify anthropogenic influence on forest carbon stocks. Using satellite-based radar imagery for such purposes has been challenged by the apparent loss of signal sensitivity to changes in forest aboveground volume (AGV) above a certain 'saturation' point. The causes of saturation are debate...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03469-3
更新日期:2017-06-14 00:00:00
abstract::This study aimed to compare the effects of volatile anesthesia and total intravenous anesthesia (TIVA) on syndecan-1 shedding in patients with gastric cancer undergoing minimally invasive gastrectomy. Patients were randomly assigned to either the Volatile (n = 68) or the TIVA (n = 68) group. Anesthesia was maintained ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81012-1
更新日期:2021-01-15 00:00:00
abstract::Porous three-dimensional (3D) polyurethane-based biocomposites were produced utilizing diatomite and hydroxyapatite as fillers. Diatomite and Hydroxyapatite (HA) were utilized to reinforce the morphological, chemical, mechanical, and thermal properties of polyurethane foam (PUF). Diatomite and Hydroxyapatite were adde...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70421-3
更新日期:2020-08-06 00:00:00
abstract::A structurally simple dielectric barrier discharge based mercury-free plasma UV-light source has been developed for efficient water disinfection. The source comprises of a dielectric barrier discharge arrangement between two co-axial quartz tubes with an optimized gas gap. The outer electrode is an aluminium baked foi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17455-2
更新日期:2017-12-12 00:00:00
abstract::Advancing the design of thin-film composite membrane surfaces is one of the most promising pathways to deal with treating varying water qualities and increase their long-term stability and permeability. Although plasma technologies have been explored for surface modification of bulk micro and ultrafiltration membrane ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29206
更新日期:2016-07-01 00:00:00
abstract::This corrects the article DOI: 10.1038/srep42627. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/srep46966
更新日期:2018-04-06 00:00:00
abstract::Selective removal of lignin and other recalcitrant compounds, collectively registered as acid-unhyrolyzable residue (AUR), results in bleaching of leaf litter, but the importance of bleaching in decomposition processes on forest soil has not been fully evaluated. The aims of this study were to elucidate the occurrence...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81206-7
更新日期:2021-01-19 00:00:00
abstract::Severely injured patients frequently suffer compromised fracture healing because of systemic post-traumatic inflammation. An important trigger of the posttraumatic immune response is the complement anaphylatoxin C5a, which acts via two receptors, C5aR1 and C5aR2, expressed on immune and bone cells. The blockade of C5a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14444-3
更新日期:2017-10-25 00:00:00
abstract::Historically, the environment has been viewed as a passive deposit of antimicrobial resistance mechanisms, where bacteria show biological cost for maintenance of these genes. Thus, in the absence of antimicrobial pressure, it is expected that they disappear from environmental bacterial communities. To test this scenar...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00600-2
更新日期:2017-03-31 00:00:00
abstract::Gradient Mn-La-Pt catalysts with three-layered structure of manganese dioxide (MnO2), lanthanum oxide (La2O3), and Platinum (Pt) for Li-O2 battery are prepared in this study. The mass ratio of the catalysts is respectively 5:2:3, 4:2:4, and 3:2:5 (MnO2: La2O3: Pt) which is start from the side of the electrolyte. The r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34950
更新日期:2016-10-12 00:00:00
abstract::Copper complexes with potent anti-tumor effect have been extensively developed. Most investigations of their modes of action focused on the biomolecular targets but not the signal transduction between target binding and cell death. We have previously shown that the cytotoxic complex pyridine(2,4-dihydroxybenzaldehyde ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36868
更新日期:2016-11-14 00:00:00