Abstract：
:In this study, an electrochemical system was established to detect the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB). A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor. The composite was characterized by scanning electron microscopy (SEM) and cyclic voltammetry (TEM). Results indicated that the MWCNTs-CS/GCE electrode exhibited higher stability and sensitivity, compared with the GCE electrode. The linear response for nicotinamide adenine dinucleotide (NADH) was 1.0-9.0 μM and the response limit was 0.12 µM. The system effectively and sensitively detected the BCAT activity by NADH concentration in the LAB culture, comparing with the optical method. The culture condition of LAB was optimized by using this system, evidencing that established method was available to detect the BCAT activity of LAB.
journal_name
Food Chemjournal_title
Food chemistryauthors
Liu R,Wang Y,Du N,Jiang D,Ge Q,Wu M,Yu H,Xu Bdoi
10.1016/j.foodchem.2019.125035subject
Has Abstractpub_date
2019-11-01 00:00:00pages
125035eissn
0308-8146issn
1873-7072pii
S0308-8146(19)31137-9journal_volume
297pub_type
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