Abstract：
:A pectin methylesterase gene (pe8F46) was cloned from Penicillium chrysogenum F46 and successfully expressed in Pichia pastoris. The full-length cDNA consists of 969 bp and encodes a 322-residue polypeptide with the calculated molecular weight of 34.1 kDa. Deduced PE8F46 belongs to family 8 of carbohydrate esterases and shares 54% identity with a functionally characterised counterpart from Myceliophthora thermophile. Purified recombinant PE8F46 showed the optimal activity at pH 5.0 and 40°C, and remained 52% maximum activity even at 10°C. An orthogonal experiment was employed to determine the best conditions for firming pineapple dices. After incubation with 0.75% (w/v) PE8F46 and 0.4% calcium lactate (w/v) for 20 min, the firmness of pineapple dices was improved by 47.6%, 13.7% higher than that of a commercial pectinase complex. These results suggest that PE8F46 has application potential in the food industry.
journal_name
Food Chemjournal_title
Food chemistryauthors
Pan X,Tu T,Wang L,Luo H,Ma R,Shi P,Meng K,Yao Bdoi
10.1016/j.foodchem.2014.04.069subject
Has Abstractpub_date
2014-11-01 00:00:00pages
229-34eissn
0308-8146issn
1873-7072pii
S0308-8146(14)00626-8journal_volume
162pub_type
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