Abstract:
:Despite recent progress in engineering native trimeric HIV-1 envelope glycoprotein (Env) mimics as vaccine candidates, Env trimers often induce vaccine-matched neutralizing antibody (NAb) responses. Understanding the specificities of autologous NAb responses and the underlying molecular mechanisms restricting the neutralization breadth is therefore informative to improve vaccine efficacy. Here, we delineate the response specificity by single B cell sorting and serum analysis of guinea pigs immunized with BG505 SOSIP.664 Env trimers. Our results reveal a prominent immune target containing both conserved and strain-specific residues in the C3/V4 region of Env in trimer-vaccinated animals. The defined NAb response shares a high degree of similarity with the early NAb response developed by a naturally infected infant from whom the HIV virus strain BG505 was isolated and later developed a broadly NAb response. Our study describes strain-specific responses and their possible evolution pathways, thereby highlighting the potential to broaden NAb responses by immunogen re-design.
journal_name
Cell Repjournal_title
Cell reportsauthors
Lei L,Yang YR,Tran K,Wang Y,Chiang CI,Ozorowski G,Xiao Y,Ward AB,Wyatt RT,Li Ydoi
10.1016/j.celrep.2019.03.039subject
Has Abstractpub_date
2019-04-09 00:00:00pages
586-598.e6issue
2issn
2211-1247pii
S2211-1247(19)30355-9journal_volume
27pub_type
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