Abstract:
:Osteoarthritic and other types of articular cartilage defects never heal on their own. Medicinal and surgical approaches are often ineffective, and the supply of autologous chondrocytes for tissue engineering is very limited. Bone marrow stromal cells (BMSCs, also known as bone marrow-derived mesenchymal stem cells) have been suggested as an adequate cell source for cartilage reconstruction. However, the majority of studies employing BMSCs for cartilage tissue engineering have used BMSCs predifferentiated into cartilage prior to implantation. This strategy has failed to achieve formation of stable, hyaline-like cartilage, resistant to hypertrophy in vivo. We hypothesized that in vitro predifferentiation of BMSCs is not necessary when cells are combined with an adequate scaffold that supports the formation of stable cartilage in vivo. In this study, naïve (undifferentiated) human BMSCs were attached to dehydrothermally crosslinked stable fibrin microbeads (FMBs) without and with other scaffolds and implanted subcutaneously into immunocompromised mice. Optimal formation of abundant, hypertrophy-resistant, ectopic hyaline-like cartilage was achieved when BMSCs were attached to FMBs covalently coated with hyaluronic acid. The cartilage that was formed was of human origin and was stable for at least 28 weeks in vivo. Stem Cells Translational Medicine 2019;8:586-592.
journal_name
Stem Cells Transl Medjournal_title
Stem cells translational medicineauthors
Kuznetsov SA,Hailu-Lazmi A,Cherman N,de Castro LF,Robey PG,Gorodetsky Rdoi
10.1002/sctm.18-0129subject
Has Abstractpub_date
2019-06-01 00:00:00pages
586-592issue
6eissn
2157-6564issn
2157-6580journal_volume
8pub_type
杂志文章abstract::Hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) play key roles in the production of mature blood cells and in the biology and clinical outcomes of hematopoietic transplants. The numbers of these cells, however, are extremely low, particularly in umbilical cord blood (UCB); thus, ex vivo expan...
journal_title:Stem cells translational medicine
pub_type: 杂志文章,评审
doi:10.5966/sctm.2013-0071
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abstract::Stem cell therapy is a promising strategy for tissue regeneration. The therapeutic benefits of cell therapy are mediated by both direct and indirect mechanisms. However, the application of stem cell therapy in the clinic is hampered by several limitations. This concise review provides a brief introduction into stem ce...
journal_title:Stem cells translational medicine
pub_type: 杂志文章,评审
doi:10.1002/sctm.17-0196
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abstract::In initial work, we developed a 14-day culture protocol under potential GMP, chemically defined conditions to generate chondroprogenitors from human embryonic stem cells (hESCs). The present study was undertaken to investigate the cartilage repair capacity of these cells. The chondrogenic protocol was optimized and va...
journal_title:Stem cells translational medicine
pub_type: 杂志文章
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.1002/sctm.17-0078
更新日期:2017-08-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2012-0086
更新日期:2012-12-01 00:00:00
abstract::Improved therapeutic assessment of experimental traumatic brain injury (TBI), using mesenchymal stem cells (MSCs), would immensely benefit its therapeutic management. Neurometabolite patterns at injury site, measured with proton magnetic resonance spectroscopy (1H-MRS) after MSCs transplantation, may serve as a bio-in...
journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2016-0087
更新日期:2017-01-01 00:00:00
abstract:UNLABELLED:The California Institute for Regenerative Medicine (CIRM) has invested approximately $70 million in programs targeting various orthopedic indications, including osteoporosis, bone fracture healing, vertebral compression fractures, and several others. The present article serves to outline the current state of...
journal_title:Stem cells translational medicine
pub_type: 杂志文章,评审
doi:10.5966/sctm.2015-0202
更新日期:2015-11-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 临床试验,杂志文章
doi:10.5966/sctm.2015-0172
更新日期:2016-05-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2015-0040
更新日期:2015-11-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.1002/sctm.17-0172
更新日期:2018-01-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2014-0103
更新日期:2015-02-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2011-0051
更新日期:2012-06-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2012-0155
更新日期:2013-08-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2015-0279
更新日期:2016-05-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2015-0052
更新日期:2015-05-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章,评审
doi:10.5966/sctm.2013-0097
更新日期:2013-12-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章,随机对照试验
doi:10.5966/sctm.2011-0052
更新日期:2012-03-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2015-0148
更新日期:2016-03-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2015-0235
更新日期:2016-09-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2015-0264
更新日期:2016-09-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章,评审
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2014-0200
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.1002/sctm.20-0019
更新日期:2020-10-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 临床试验,杂志文章
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更新日期:2017-08-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.1002/sctm.17-0149
更新日期:2018-01-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.1002/sctm.20-0224
更新日期:2020-11-04 00:00:00
abstract::Bone marrow mesenchymal stem cells (BMSCs) have been shown to offer a wide variety of cellular functions including the protective effects on damaged hearts. Here we investigated the antiaging properties of BMSCs and the underlying mechanism in a cellular model of cardiomyocyte senescence and a rat model of aging heart...
journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2014-0206
更新日期:2015-05-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2013-0035
更新日期:2013-09-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2013-0216
更新日期:2014-07-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.1002/sctm.18-0025
更新日期:2018-12-01 00:00:00