Concise review: ex vivo expansion of cord blood-derived hematopoietic stem and progenitor cells: basic principles, experimental approaches, and impact in regenerative medicine.

abstract：:Tissue regeneration using progenitor cell-based therapy has the potential to aid in the healing of a diverse range of pathologies, ranging from short-gut syndrome to spinal cord lesions. However, there are numerous hurdles to be overcome prior to the widespread application of these cells in the clinical setting. One o...

journal_title：Stem cells translational medicine

authors： Hyun J,Grova M,Nejadnik H,Lo D,Morrison S,Montoro D,Chung M,Zimmermann A,Walmsley GG,Lee M,Daldrup-Link H,Wan DC,Longaker MT

更新日期：2013-09-01 00:00:00

abstract：:The immunomodulatory activity of mesenchymal stem/stromal cells (MSCs) to suppress innate and adaptive immune responses offers a potent cell therapy for modulating inflammation and promoting tissue regeneration. However, the inflammatory cytokine milieu plays a critical role in stimulating MSC immunomodulatory activit...

journal_title：Stem cells translational medicine

authors： Zimmermann JA,Hettiaratchi MH,McDevitt TC

更新日期：2017-01-01 00:00:00

abstract：UNLABELLED:Cell secretion is an important mechanism for stem cell-based therapeutic angiogenesis, along with cell differentiation to vascular endothelial cells or smooth muscle cells. Cell-released microvesicles (MVs) have been recently implicated to play an essential role in intercellular communication. The purpose of...

journal_title：Stem cells translational medicine

authors： Kang T,Jones TM,Naddell C,Bacanamwo M,Calvert JW,Thompson WE,Bond VC,Chen YE,Liu D

更新日期：2016-04-01 00:00:00

abstract：:Human pluripotent stem cells have the potential to promote biological studies and accelerate drug discovery efforts by making possible direct experimentation on a variety of human cell types of interest. However, stem cell cultures are generally heterogeneous and efficient differentiation and purification protocols ar...

journal_title：Stem cells translational medicine

authors： Sluch VM,Chamling X,Liu MM,Berlinicke CA,Cheng J,Mitchell KL,Welsbie DS,Zack DJ

更新日期：2017-11-01 00:00:00

abstract：:: Fast remyelination by endogenous oligodendrocyte precursor cells (OPCs) is essential to prevent axonal and subsequent retrograde neuronal degeneration in demyelinating lesions in multiple sclerosis (MS). In chronic lesions, however, the remyelination capacity of OPCs becomes insufficient. Cell therapy with exogenous...

journal_title：Stem cells translational medicine

authors： Thiruvalluvan A,Czepiel M,Kap YA,Mantingh-Otter I,Vainchtein I,Kuipers J,Bijlard M,Baron W,Giepmans B,Brück W,'t Hart BA,Boddeke E,Copray S

更新日期：2016-11-01 00:00:00

abstract：:Cell-based therapies have gained interest as a potential treatment method in cardiovascular disease in the past two decades, peripheral artery disease amongst others. Initial pre-clinical and small pilot clinical studies showed promising effects of cell therapy in peripheral artery disease and chronic limb-threatening...

journal_title：Stem cells translational medicine

authors： Teraa M,Gremmels H,Wijnand JGJ,Verhaar MC

更新日期：2018-12-01 00:00:00

abstract：:Techniques that enable longitudinal tracking of cell fate after myocardial delivery are imperative for optimizing the efficacy of cell-based cardiac therapies. However, these approaches have been underutilized in preclinical models and clinical trials, and there is considerable demand for site-specific strategies achi...

journal_title：Stem cells translational medicine

authors： Ostrominski JW,Yada RC,Sato N,Klein M,Blinova K,Patel D,Valadez R,Palisoc M,Pittaluga S,Peng KW,San H,Lin Y,Basuli F,Zhang X,Swenson RE,Haigney M,Choyke PL,Zou J,Boehm M,Hong SG,Dunbar CE

更新日期：2020-10-01 00:00:00

abstract：:We conducted a phase II, noncomparative, multicenter study to assess the efficacy and safety of allogeneic bone marrow-derived mesenchymal stromal cells (BM-MSCs) expanded in vitro for patients with aplastic anemia (AA) refractory to immunosuppressive therapy. Seventy-four patients from seven centers received allogene...

journal_title：Stem cells translational medicine

authors： Pang Y,Xiao HW,Zhang H,Liu ZH,Li L,Gao Y,Li HB,Jiang ZJ,Tan H,Lin JR,Du X,Weng JY,Nie DN,Lin DJ,Zhang XZ,Liu QF,Xu DR,Chen HJ,Ge XH,Wang XY,Xiao Y

更新日期：2017-07-01 00:00:00

abstract：:Improved therapeutic assessment of experimental traumatic brain injury (TBI), using mesenchymal stem cells (MSCs), would immensely benefit its therapeutic management. Neurometabolite patterns at injury site, measured with proton magnetic resonance spectroscopy (1H-MRS) after MSCs transplantation, may serve as a bio-in...

journal_title：Stem cells translational medicine

authors： Mishra SK,Rana P,Khushu S,Gangenahalli G

更新日期：2017-01-01 00:00:00

abstract：:Standardization guidelines for human pluripotent stem cells are still very broadly defined, despite ongoing clinical trials in the U.S., U.K., and Japan. The requirements for validation of human embryonic (hESCs) and induced pluripotent stem cells (iPSCs) in general follow the regulations for other clinically complian...

journal_title：Stem cells translational medicine

authors： Devito L,Petrova A,Miere C,Codognotto S,Blakely N,Lovatt A,Ogilvie C,Khalaf Y,Ilic D

更新日期：2014-10-01 00:00:00

abstract：UNLABELLED:: Recapitulation of endochondral ossification (ECO) (i.e., generation of marrow-containing ossicles through a cartilage intermediate) has relevance to develop human organotypic models for bone or hematopoietic cells and to engineer grafts for bone regeneration. Unlike bone marrow-derived stromal cells (also ...

journal_title：Stem cells translational medicine

authors： Osinga R,Di Maggio N,Todorov A,Allafi N,Barbero A,Laurent F,Schaefer DJ,Martin I,Scherberich A

更新日期：2016-08-01 00:00:00

abstract：:The kidney is a specialized low-regenerative organ with several different types of cellular lineages; however, the identity of renal stem/progenitor cells with nephrogenic potential and their preferred niche(s) are largely unknown and debated. Most of the therapeutic approaches to kidney regeneration are based on admi...

journal_title：Stem cells translational medicine

authors： Aggarwal S,Moggio A,Bussolati B

更新日期：2013-12-01 00:00:00

abstract：:Adipose-derived stem cells (ASCs) are being tested in clinical trials related to cell-based regenerative therapies. Although most of the current expansion protocols for ASCs use fetal calf serum (FCS), xenogeneic-free medium supplements are greatly desired. This study aims to compare the effect of FCS, human platelet ...

journal_title：Stem cells translational medicine

authors： Riis S,Nielsen FM,Pennisi CP,Zachar V,Fink T

更新日期：2016-03-01 00:00:00

abstract：UNLABELLED:: This study explored a new approach to enhance aneurysm (AN) neck endothelialization via erythropoietin (EPO)-induced endothelial progenitor cell (EPC) stimulation. Results suggest that EPO enhanced the endothelialization of a coiled embolization AN neck by stimulating EPCs via vascular endothelial growth f...

journal_title：Stem cells translational medicine

authors： Liu P,Zhou Y,An Q,Song Y,Chen X,Yang GY,Zhu W

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:Tendon or ligament tears can decrease patients' quality of life. Many therapeutic interventions are available to treat such injuries. Mesenchymal stem cells (MSCs) have been shown to be effective in treating tendon or ligament tears; however, the use of stem cell-conditioned medium (CM) requires further inve...

journal_title：Stem cells translational medicine

authors： Rhatomy S,Prasetyo TE,Setyawan R,Soekarno NR,Romaniyanto F,Sedjati AP,Sumarwoto T,Utomo DN,Suroto H,Mahyudin F,Prakoeswa CRS

更新日期：2020-08-01 00:00:00

abstract：:Limbal stem cell (LSC) deficiency is a visually debilitating condition caused by abnormal maintenance of LSCs. It is treated by transplantation of donor-derived limbal epithelial cells (LECs), the success of which depends on the presence and quality of LSCs within the transplant. Understanding the immunobiological res...

journal_title：Stem cells translational medicine

authors： Shaharuddin B,Ahmad S,Md Latar N,Ali S,Meeson A

更新日期：2017-03-01 00:00:00

abstract：:Patient-specific induced pluripotent stem cells (iPSCs) hold great promise for many applications, including disease modeling to elucidate mechanisms involved in disease pathogenesis, drug screening, and ultimately regenerative medicine therapies. A frequently used starting source of cells for reprogramming has been de...

journal_title：Stem cells translational medicine

authors： Barrett R,Ornelas L,Yeager N,Mandefro B,Sahabian A,Lenaeus L,Targan SR,Svendsen CN,Sareen D

更新日期：2014-12-01 00:00:00

abstract：:The development of a California-based induced pluripotent stem cell (iPSC) bank based on human leukocyte antigen (HLA) haplotype matching represents a significant challenge and a valuable opportunity for the advancement of regenerative medicine. However, previously published models of iPSC banks have neither addressed...

journal_title：Stem cells translational medicine

authors： Pappas DJ,Gourraud PA,Le Gall C,Laurent J,Trounson A,DeWitt N,Talib S

更新日期：2015-05-01 00:00:00

abstract：UNLABELLED:Histone deacetylase (HDAC) inhibitors possess therapeutic potential to reverse aberrant epigenetic changes associated with cancers, neurological diseases, and immune disorders. Unfortunately, clinical studies with some HDAC inhibitors displayed delayed cardiac adverse effects, such as atrial fibrillation and...

journal_title：Stem cells translational medicine

authors： Kopljar I,Gallacher DJ,De Bondt A,Cougnaud L,Vlaminckx E,Van den Wyngaert I,Lu HR

更新日期：2016-05-01 00:00:00

abstract：:Muscle-derived stem cells (MDSCs) isolated from mouse skeletal muscle by a modified preplate technique exhibit long-term proliferation, high self-renewal, and multipotent differentiation capabilities in vitro. MDSCs retrovirally transduced to express bone morphogenetic proteins (BMPs) can differentiate into osteocytes...

journal_title：Stem cells translational medicine

authors： Li H,Johnson NR,Usas A,Lu A,Poddar M,Wang Y,Huard J

更新日期：2013-09-01 00:00:00

abstract：:Despite approaches in regenerative medicine using stem cells, bio-engineered scaffolds, and targeted drug delivery to enhance human tissue repair, clinicians remain unable to regenerate large-scale, multi-tissue defects in situ. The study of regenerative biology using mammalian models of complex tissue regeneration of...

journal_title：Stem cells translational medicine

authors： Simkin J,Seifert AW

更新日期：2018-02-01 00:00:00

abstract：:Animal models show that systemically administered bone marrow-derived mesenchymal stem cells (MSCs) home to sites of primary and metastatic prostate cancer (PC)-making them candidates to selectively deliver cytotoxic agents. To further assess this potential as a cell-based therapeutic vehicle, a phase I study testing ...

journal_title：Stem cells translational medicine

authors： Schweizer MT,Wang H,Bivalacqua TJ,Partin AW,Lim SJ,Chapman C,Abdallah R,Levy O,Bhowmick NA,Karp JM,De Marzo A,Isaacs JT,Brennen WN,Denmeade SR

更新日期：2019-05-01 00:00:00

abstract：:Human-induced pluripotent stem cells (hiPSCs)-based cell therapy holds promise for treating stress urinary incontinence (SUI). However, safety concerns, especially tumorgenic potential of residual undifferentiated cells in hiPSC derivatives, are major barriers for its clinical translation. An efficient, fast and clini...

journal_title：Stem cells translational medicine

authors： Li Y,Green M,Wen Y,Wei Y,Wani P,Wang Z,Reijo Pera R,Chen B

更新日期：2017-04-01 00:00:00

abstract：:The present first-in-human clinical trial evaluated the safety and feasibility of a newly developed and cryopreserved Cardiology Stem Cell Centre adipose-derived stromal cell (CSCC_ASC) product from healthy donors for intramyocardial injection in ten patients with ischemic heart disease and ischemic heart failure (IHF...

journal_title：Stem cells translational medicine

authors： Kastrup J,Haack-Sørensen M,Juhl M,Harary Søndergaard R,Follin B,Drozd Lund L,Mønsted Johansen E,Ali Qayyum A,Bruun Mathiasen A,Jørgensen E,Helqvist S,Jørgen Elberg J,Bruunsgaard H,Ekblond A

更新日期：2017-11-01 00:00:00

abstract：:The potential of autologous cell-based therapies including those using multipotent mesenchymal stromal cells (MSCs) is being investigated for multiple sclerosis (MS) and other neurological conditions. However, the phenotype of MSC in neurological diseases has not been fully characterized. We have previously shown that...

journal_title：Stem cells translational medicine

authors： Redondo J,Sarkar P,Kemp K,Heesom KJ,Wilkins A,Scolding NJ,Rice CM

更新日期：2018-10-01 00:00:00

abstract：UNLABELLED:: Chronic rotator cuff (RC) tears are a common and debilitating injury, characterized by dramatic expansion of adipose tissue, muscle atrophy, and limited functional recovery. The role of adipose expansion in RC pathology is unknown; however, given the identified paracrine/endocrine regulation by other adipo...

journal_title：Stem cells translational medicine

authors： Meyer GA,Gibbons MC,Sato E,Lane JG,Ward SR,Engler AJ

更新日期：2015-07-01 00:00:00

abstract：:Strategies for musculoskeletal tissue regeneration apply adult mesenchymal stem/stromal cells (MSCs) that can be sourced from bone marrow- and lipo-aspirates. Adipose tissue-derived MSCs are more easily harvested in the large quantities required for skeletal tissue-engineering approaches, but are generally considered ...

journal_title：Stem cells translational medicine

authors： Samsonraj RM,Dudakovic A,Manzar B,Sen B,Dietz AB,Cool SM,Rubin J,van Wijnen AJ

更新日期：2018-02-01 00:00:00

abstract：:Niemann-Pick disease, type C1 (NPC1) is a familial disorder that has devastating consequences on postnatal development with multisystem effects, including neurodegeneration. There is no Food and Drug Administration-approved treatment option for NPC1; however, several potentially therapeutic compounds have been identif...

journal_title：Stem cells translational medicine

authors： Efthymiou AG,Steiner J,Pavan WJ,Wincovitch S,Larson DM,Porter FD,Rao MS,Malik N

更新日期：2015-03-01 00:00:00

abstract：:Osteoarthritic and other types of articular cartilage defects never heal on their own. Medicinal and surgical approaches are often ineffective, and the supply of autologous chondrocytes for tissue engineering is very limited. Bone marrow stromal cells (BMSCs, also known as bone marrow-derived mesenchymal stem cells) h...

journal_title：Stem cells translational medicine

authors： Kuznetsov SA,Hailu-Lazmi A,Cherman N,de Castro LF,Robey PG,Gorodetsky R

更新日期：2019-06-01 00:00:00

abstract：:Human induced pluripotent stem cells (hiPSCs) have generated a great deal of attention owing to their capacity for self-renewal and differentiation into the three germ layers of the body. Their discovery has facilitated a new era in biomedicine for understanding human development, drug screening, disease modeling, and...

journal_title：Stem cells translational medicine

authors： Borys BS,So T,Colter J,Dang T,Roberts EL,Revay T,Larijani L,Krawetz R,Lewis I,Argiropoulos B,Rancourt DE,Jung S,Hashimura Y,Lee B,Kallos MS

更新日期：2020-09-01 00:00:00