Adipose-Derived Stem Cells Induce Angiogenesis via Microvesicle Transport of miRNA-31.

Abstract:

UNLABELLED:Cell secretion is an important mechanism for stem cell-based therapeutic angiogenesis, along with cell differentiation to vascular endothelial cells or smooth muscle cells. Cell-released microvesicles (MVs) have been recently implicated to play an essential role in intercellular communication. The purpose of this study was to explore the potential effects of stem cell-released MVs in proangiogenic therapy. We observed for the first time that MVs were released from adipose-derived stem cells (ASCs) and were able to increase the migration and tube formation of human umbilical vein endothelial cells (HUVECs). Endothelial differentiation medium (EDM) preconditioning of ASCs upregulated the release of MVs and enhanced the angiogenic effect of the released MVs in vitro. RNA analysis revealed that microRNA was enriched in ASC-released MVs and that the level of microRNA-31 (miR-31) in MVs was notably elevated upon EDM-preconditioning of MV-donor ASCs. Further studies exhibited that miR-31 in MVs contributed to the migration and tube formation of HUVECs, microvessel outgrowth of mouse aortic rings, and vascular formation of mouse Matrigel plugs. Moreover, factor-inhibiting HIF-1, an antiangiogenic gene, was identified as the target of miR-31 in HUVECs. Our findings provide the first evidence that MVs from ASCs, particularly from EDM-preconditioned ASCs, promote angiogenesis and the delivery of miR-31 may contribute the proangiogenic effect. SIGNIFICANCE:This study provides the evidence that microvesicles (MVs) from adipose-derived stem cells (ASCs), particularly from endothelial differentiation medium (EDM)-preconditioned ASCs, promote angiogenesis. An underlying mechanism of the proangiogenesis may be the delivery of microRNA-31 via MVs from ASCs to vascular endothelial cells in which factor-inhibiting HIF-1 is targeted and suppressed. The study findings reveal the role of MVs in mediating ASC-induced angiogenesis and suggest a potential MV-based angiogenic therapy for ischemic diseases.

journal_name

Stem Cells Transl Med

authors

Kang T,Jones TM,Naddell C,Bacanamwo M,Calvert JW,Thompson WE,Bond VC,Chen YE,Liu D

doi

10.5966/sctm.2015-0177

subject

Has Abstract

pub_date

2016-04-01 00:00:00

pages

440-50

issue

4

eissn

2157-6564

issn

2157-6580

pii

sctm.2015-0177

journal_volume

5

pub_type

杂志文章
  • Gaucher Disease-Induced Pluripotent Stem Cells Display Decreased Erythroid Potential and Aberrant Myelopoiesis.

    abstract::Gaucher disease (GD) is the most common lysosomal storage disease resulting from mutations in the lysosomal enzyme glucocerebrosidase (GCase). The hematopoietic abnormalities in GD include the presence of characteristic Gaucher macrophages that infiltrate patient tissues and cytopenias. At present, it is not clear whe...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2014-0213

    authors: Sgambato JA,Park TS,Miller D,Panicker LM,Sidransky E,Lun Y,Awad O,Bentzen SM,Zambidis ET,Feldman RA

    更新日期:2015-08-01 00:00:00

  • PTEN Promotes Dopaminergic Neuronal Differentiation Through Regulation of ERK-Dependent Inhibition of S6K Signaling in Human Neural Stem Cells.

    abstract::: Phosphatase and tension homolog (PTEN) is a widely known negative regulator of insulin/phosphatidylinositol 3-kinase (PI3K) signaling. The PI3K/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) and Ras-extracellular signal-regulated kinase (Ras-ERK) signaling pathways are the chief mechanisms controlling the surviva...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2015-0200

    authors: Lee JE,Lim MS,Park JH,Park CH,Koh HC

    更新日期:2016-10-01 00:00:00

  • Human pluripotent stem cell-derived radial glia recapitulate developmental events and provide real-time access to cortical neurons and astrocytes.

    abstract::Studies of human cerebral cortex development are limited by difficulties in accessing and manipulating human neural tissue at specific development stages. We have derived human radial glia (hRG), which are responsible for most cerebral cortex neurogenesis, from human pluripotent stem cells. These hRG display the hallm...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2014-0137

    authors: Duan L,Peng CY,Pan L,Kessler JA

    更新日期:2015-05-01 00:00:00

  • The Infrapatellar Fat Pad as a Source of Perivascular Stem Cells with Increased Chondrogenic Potential for Regenerative Medicine.

    abstract::Perivascular stem cells (PSCs) are the natural ancestors of mesenchymal stem cells (MSCs) and are the stem cells responsible for homeostasis and repair in vivo. Prospectively identified and isolated PSCs have demonstrated increased plasticity and osteogenic potential. Cells from the infrapatellar fat pad (IFP) have de...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2016-0040

    authors: Hindle P,Khan N,Biant L,Péault B

    更新日期:2017-01-01 00:00:00

  • Adipose tissue-derived multipotent stromal cells have a higher immunomodulatory capacity than their bone marrow-derived counterparts.

    abstract::Adipose tissue-derived multipotent stromal cells (AT-MSCs) are studied as an alternative to bone marrow-derived multipotent stromal cells (BM-MSCs) for immunomodulatory treatment. In this study, we systematically compared the immunomodulatory capacities of BM-MSCs and AT-MSCs derived from age-matched donors. We found ...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2012-0184

    authors: Melief SM,Zwaginga JJ,Fibbe WE,Roelofs H

    更新日期:2013-06-01 00:00:00

  • Sustained release of bone morphogenetic protein 2 via coacervate improves the osteogenic potential of muscle-derived stem cells.

    abstract::Muscle-derived stem cells (MDSCs) isolated from mouse skeletal muscle by a modified preplate technique exhibit long-term proliferation, high self-renewal, and multipotent differentiation capabilities in vitro. MDSCs retrovirally transduced to express bone morphogenetic proteins (BMPs) can differentiate into osteocytes...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2013-0027

    authors: Li H,Johnson NR,Usas A,Lu A,Poddar M,Wang Y,Huard J

    更新日期:2013-09-01 00:00:00

  • Enhanced Immunosuppression of T Cells by Sustained Presentation of Bioactive Interferon-γ Within Three-Dimensional Mesenchymal Stem Cell Constructs.

    abstract::The immunomodulatory activity of mesenchymal stem/stromal cells (MSCs) to suppress innate and adaptive immune responses offers a potent cell therapy for modulating inflammation and promoting tissue regeneration. However, the inflammatory cytokine milieu plays a critical role in stimulating MSC immunomodulatory activit...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2016-0044

    authors: Zimmermann JA,Hettiaratchi MH,McDevitt TC

    更新日期:2017-01-01 00:00:00

  • Immature Midbrain Dopaminergic Neurons Derived from Floor-Plate Method Improve Cell Transplantation Therapy Efficacy for Parkinson's Disease.

    abstract::Recent reports have indicated human embryonic stem cells-derived midbrain dopamine (mDA) neurons as proper cell resources for use in Parkinson's disease (PD) therapy. Nevertheless, no detailed and systematic study has been conducted to identify which differentiation stages of mDA cells are most suitable for transplant...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.1002/sctm.16-0470

    authors: Qiu L,Liao MC,Chen AK,Wei S,Xie S,Reuveny S,Zhou ZD,Hunziker W,Tan EK,Oh SKW,Zeng L

    更新日期:2017-09-01 00:00:00

  • Variability in the generation of induced pluripotent stem cells: importance for disease modeling.

    abstract::In the field of disease modeling, induced pluripotent stem cells (iPSCs) have become an appealing choice, especially for diseases that do not have an animal model. They can be generated from patients with known clinical features and compared with cells from healthy controls to identify the biological bases of disease....

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2012-0043

    authors: Vitale AM,Matigian NA,Ravishankar S,Bellette B,Wood SA,Wolvetang EJ,Mackay-Sim A

    更新日期:2012-09-01 00:00:00

  • Cross talk between mesenchymal and glioblastoma stem cells: Communication beyond controversies.

    abstract::Mesenchymal stem cells (MSCs) can be isolated from bone marrow or other adult tissues (adipose tissue, dental pulp, amniotic fluid, and umbilical cord). In vitro, MSCs grow as adherent cells, display fibroblast-like morphology, and self-renew, undergoing specific mesodermal differentiation. High heterogeneity of MSCs ...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章,评审

    doi:10.1002/sctm.20-0161

    authors: Bajetto A,Thellung S,Dellacasagrande I,Pagano A,Barbieri F,Florio T

    更新日期:2020-11-01 00:00:00

  • Concise Review: Translating Regenerative Biology into Clinically Relevant Therapies: Are We on the Right Path?

    abstract::Despite approaches in regenerative medicine using stem cells, bio-engineered scaffolds, and targeted drug delivery to enhance human tissue repair, clinicians remain unable to regenerate large-scale, multi-tissue defects in situ. The study of regenerative biology using mammalian models of complex tissue regeneration of...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章,评审

    doi:10.1002/sctm.17-0213

    authors: Simkin J,Seifert AW

    更新日期:2018-02-01 00:00:00

  • Alteration of naïve and memory B-cell subset in chronic graft-versus-host disease patients after treatment with mesenchymal stromal cells.

    abstract::Although mesenchymal stromal cells (MSCs) possess immunomodulatory properties and exhibit promising efficacy against chronic graft-versus-host disease (cGVHD), little is known about the immune changes by which MSCs ameliorate cGVHD in vivo. Recent studies have suggested that B lymphocytes might play an important role ...

    journal_title:Stem cells translational medicine

    pub_type: 临床试验,杂志文章

    doi:10.5966/sctm.2014-0001

    authors: Peng Y,Chen X,Liu Q,Xu D,Zheng H,Liu L,Liu Q,Liu M,Fan Z,Sun J,Li X,Zou R,Xiang AP

    更新日期:2014-09-01 00:00:00

  • Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid-induced osteonecrosis of the femoral head.

    abstract::Steroid-induced osteonecrosis of the femoral head (ONFH) is characterized by decreased osteogenesis, angiogenesis, and increased adipogenesis. While bone tissue engineering has been widely investigated to treat ONFH, its therapeutic effects remain unsatisfactory. Therefore, further studies are required to determine op...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.1002/sctm.20-0346

    authors: Xu H,Wang C,Liu C,Peng Z,Li J,Jin Y,Wang Y,Guo J,Zhu L

    更新日期:2021-01-13 00:00:00

  • Intra-articular transplantation of atsttrin-transduced mesenchymal stem cells ameliorate osteoarthritis development.

    abstract::Osteoarthritis (OA) remains an intractable clinical challenge. Few drugs are available for reversing this degenerative disease, although some promising candidates have performed well in preclinical studies. Tumor necrosis factor α (TNFα) has been identified as a crucial effector modulating OA pathogenesis. This study ...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2014-0200

    authors: Xia Q,Zhu S,Wu Y,Wang J,Cai Y,Chen P,Li J,Heng BC,Ouyang HW,Lu P

    更新日期:2015-05-01 00:00:00

  • Oral Mucosal Lamina Propria-Progenitor Cells Exert Antibacterial Properties via the Secretion of Osteoprotegerin and Haptoglobin.

    abstract:UNLABELLED:The oral cavity possesses a diverse microflora, yet recurrent infections within healthy individuals are rare. Wound healing within the buccal mucosa is preferential, potentially because of the presence of oral mucosal lamina propria-progenitor cells (OMLP-PCs). In addition to their multipotency, OMLP-PCs dem...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2015-0043

    authors: Board-Davies E,Moses R,Sloan A,Stephens P,Davies LC

    更新日期:2015-11-01 00:00:00

  • Proceedings: human leukocyte antigen haplo-homozygous induced pluripotent stem cell haplobank modeled after the california population: evaluating matching in a multiethnic and admixed population.

    abstract::The development of a California-based induced pluripotent stem cell (iPSC) bank based on human leukocyte antigen (HLA) haplotype matching represents a significant challenge and a valuable opportunity for the advancement of regenerative medicine. However, previously published models of iPSC banks have neither addressed...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2015-0052

    authors: Pappas DJ,Gourraud PA,Le Gall C,Laurent J,Trounson A,DeWitt N,Talib S

    更新日期:2015-05-01 00:00:00

  • Vascular Smooth Muscle Cells From Hypertensive Patient-Derived Induced Pluripotent Stem Cells to Advance Hypertension Pharmacogenomics.

    abstract:UNLABELLED:Studies in hypertension (HTN) pharmacogenomics seek to identify genetic sources of variable antihypertensive drug response. Genetic association studies have detected single-nucleotide polymorphisms (SNPs) that link to drug responses; however, to understand mechanisms underlying how genetic traits alter drug ...

    journal_title:Stem cells translational medicine

    pub_type: 临床试验,杂志文章

    doi:10.5966/sctm.2015-0126

    authors: Biel NM,Santostefano KE,DiVita BB,El Rouby N,Carrasquilla SD,Simmons C,Nakanishi M,Cooper-DeHoff RM,Johnson JA,Terada N

    更新日期:2015-12-01 00:00:00

  • Concise review: pluripotent stem cell-based regenerative applications for failing β-cell function.

    abstract::Diabetes engenders the loss of pancreatic β-cell mass and/or function, resulting in insulin deficiency relative to the metabolic needs of the body. Diabetic care has traditionally relied on pharmacotherapy, exemplified by insulin replacement to target peripheral actions of the hormone. With growing understanding of th...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章,评审

    doi:10.5966/sctm.2013-0184

    authors: Holditch SJ,Terzic A,Ikeda Y

    更新日期:2014-05-01 00:00:00

  • Epimuscular Fat in the Human Rotator Cuff Is a Novel Beige Depot.

    abstract:UNLABELLED:: Chronic rotator cuff (RC) tears are a common and debilitating injury, characterized by dramatic expansion of adipose tissue, muscle atrophy, and limited functional recovery. The role of adipose expansion in RC pathology is unknown; however, given the identified paracrine/endocrine regulation by other adipo...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2014-0287

    authors: Meyer GA,Gibbons MC,Sato E,Lane JG,Ward SR,Engler AJ

    更新日期:2015-07-01 00:00:00

  • Umbilical cord blood: The promise and the uncertainty.

    abstract::Unfortunately, many patients referred for hematopoietic cell transplant will not have a fully matched related donor, and finding matched unrelated donors through the registry may be difficult, especially if the recipient is not of Northern European descent [N Engl J Med 2014;371:339-348]. Umbilical cord blood (UCB) ha...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章,评审

    doi:10.1002/sctm.19-0288

    authors: Kindwall-Keller TL,Ballen KK

    更新日期:2020-10-01 00:00:00

  • A Comparative Analysis of Attitudes on Communication Toward Stem Cell Research and Regenerative Medicine Between the Public and the Scientific Community.

    abstract::Owing to the rapid progress in stem cell research (SCR) and regenerative medicine (RM), society's expectation and interest in these fields are increasing. For effective communication on issues concerning SCR and RM, surveys for understanding the interests of stakeholders is essential. For this purpose, we conducted a ...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.1002/sctm.17-0184

    authors: Shineha R,Inoue Y,Ikka T,Kishimoto A,Yashiro Y

    更新日期:2018-02-01 00:00:00

  • Anti-inflammatory mesenchymal stem cells (MSC2) attenuate symptoms of painful diabetic peripheral neuropathy.

    abstract::Mesenchymal stem cells (MSCs) are very attractive candidates in cell-based strategies that target inflammatory diseases. Preclinical animal studies and many clinical trials have demonstrated that human MSCs can be safely administered and that they modify the inflammatory process in the targeted injured tissue. Our lab...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2012-0025

    authors: Waterman RS,Morgenweck J,Nossaman BD,Scandurro AE,Scandurro SA,Betancourt AM

    更新日期:2012-07-01 00:00:00

  • Convergence of Cell Pharmacology and Drug Delivery.

    abstract::Cellular therapy is enabling new approaches to tackle significant unmet needs in areas such as regenerative medicine and immunotherapy. The pharmacology of cell therapeutics becomes of critical importance to assure that these new drugs work reproducibly and effectively. Cell pharmacology can benefit from adapting prin...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.1002/sctm.19-0019

    authors: Aijaz A,Vaninov N,Allen A,Barcia RN,Parekkadan B

    更新日期:2019-09-01 00:00:00

  • Cartilage repair using human embryonic stem cell-derived chondroprogenitors.

    abstract::In initial work, we developed a 14-day culture protocol under potential GMP, chemically defined conditions to generate chondroprogenitors from human embryonic stem cells (hESCs). The present study was undertaken to investigate the cartilage repair capacity of these cells. The chondrogenic protocol was optimized and va...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2014-0101

    authors: Cheng A,Kapacee Z,Peng J,Lu S,Lucas RJ,Hardingham TE,Kimber SJ

    更新日期:2014-11-01 00:00:00

  • Concise Review: Exploiting Unique Biological Features of Leukemia Stem Cells for Therapeutic Benefit.

    abstract::Cancer stem cells play a critical role in disease initiation and insensitivity to chemotherapy in numerous hematologic malignancies and some solid tumors, and these stem cells need to be eradicated to achieve a cure. Key to successful targeting of cancer stem cells is to identify and functionally test critical target ...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章,评审

    doi:10.1002/sctm.18-0247

    authors: Zhang H,Li S

    更新日期:2019-08-01 00:00:00

  • Hierarchy of breast cancer cells: key to reverse dormancy for therapeutic intervention.

    abstract::An understanding of how cancer cells adapt dormancy would allow for targeted treatment. The current literature suggests that the cancer stem cells might be the major cells with the ability to become quiescent and to resist current drug treatment. The properties of cancer stem cells and healthy stem cells are functiona...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章,评审

    doi:10.5966/sctm.2014-0013

    authors: Bliss SA,Greco SJ,Rameshwar P

    更新日期:2014-07-01 00:00:00

  • Comparison of Different Cytokine Conditions Reveals Resveratrol as a New Molecule for Ex Vivo Cultivation of Cord Blood-Derived Hematopoietic Stem Cells.

    abstract:UNLABELLED:Human cord blood (CB)-derived hematopoietic stem cells (HSCs) are an interesting source for HSC transplantation. However, the number of collected CB-HSCs is often too low for one transplantation; therefore, ex vivo expansion of CB-HSCs is desirable. Current expansion protocols are based on the use of cytokin...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2014-0284

    authors: Heinz N,Ehrnström B,Schambach A,Schwarzer A,Modlich U,Schiedlmeier B

    更新日期:2015-09-01 00:00:00

  • Concise Review: Is Cardiac Cell Therapy Dead? Embarrassing Trial Outcomes and New Directions for the Future.

    abstract::Stem cell therapy is a promising strategy for tissue regeneration. The therapeutic benefits of cell therapy are mediated by both direct and indirect mechanisms. However, the application of stem cell therapy in the clinic is hampered by several limitations. This concise review provides a brief introduction into stem ce...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章,评审

    doi:10.1002/sctm.17-0196

    authors: Tang JN,Cores J,Huang K,Cui XL,Luo L,Zhang JY,Li TS,Qian L,Cheng K

    更新日期:2018-04-01 00:00:00

  • Fos Promotes Early Stage Teno-Lineage Differentiation of Tendon Stem/Progenitor Cells in Tendon.

    abstract::Stem cells have been widely used in tendon tissue engineering. The lack of refined and controlled differentiation strategy hampers the tendon repair and regeneration. This study aimed to find new effective differentiation factors for stepwise tenogenic differentiation. By microarray screening, the transcript factor Fo...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.1002/sctm.15-0146

    authors: Chen J,Zhang E,Zhang W,Liu Z,Lu P,Zhu T,Yin Z,Backman LJ,Liu H,Chen X,Ouyang H

    更新日期:2017-11-01 00:00:00

  • Therapeutic Prospective of Infused Allogenic Cultured Mesenchymal Stem Cells in Traumatic Brain Injury Mice: A Longitudinal Proton Magnetic Resonance Spectroscopy Assessment.

    abstract::Improved therapeutic assessment of experimental traumatic brain injury (TBI), using mesenchymal stem cells (MSCs), would immensely benefit its therapeutic management. Neurometabolite patterns at injury site, measured with proton magnetic resonance spectroscopy (1H-MRS) after MSCs transplantation, may serve as a bio-in...

    journal_title:Stem cells translational medicine

    pub_type: 杂志文章

    doi:10.5966/sctm.2016-0087

    authors: Mishra SK,Rana P,Khushu S,Gangenahalli G

    更新日期:2017-01-01 00:00:00