Abstract:
:Global cerebral ischemia (GCI) is the leading cause of a poor prognosis even after successful resuscitation from cardiac arrest. Therapeutic induction of hypothermia (TH) is the only proven therapy-and current standard care-for GCI after cardiac arrest; however, its application has been significantly limited owing to technical difficulties. Mesenchymal stem cells (MSCs) are known to suppress neuronal death after cerebral ischemia. The prevention of blood-brain barrier (BBB) disruption has not been suggested as a mechanism of MSC treatment but has for TH. We evaluated the therapeutic effect of MSC administration on BBB disruption and neutrophil infiltration after GCI. To evaluate the therapeutic effects of MSC treatment, rats were subjected to 7 minutes of transient GCI and treated with MSCs immediately after reperfusion. Hippocampal neuronal death was evaluated at 7 days after ischemia using Fluoro-Jade B (FJB). BBB disruption, endothelial damage, and neutrophil infiltration were evaluated at 7 days after ischemia by immunostaining for IgG leakage, Rat endothelial antigen-1, and myeloperoxidase (MPO). Rats treated with MSCs showed a significantly reduced FJB+ neuron count compared with the control group. They also showed reduced IgG leakage, endothelial damage, and MPO+ cell counts. The present study demonstrated that administration of MSCs after transient GCI provides a dramatic protective effect against hippocampal neuronal death. We hypothesized that the neuroprotective effects of MSC treatment might be associated with the prevention of BBB disruption and endothelial damage and a decrease in neutrophil infiltration.
journal_name
Stem Cells Transl Medjournal_title
Stem cells translational medicineauthors
Chung TN,Kim JH,Choi BY,Chung SP,Kwon SW,Suh SWdoi
10.5966/sctm.2014-0103subject
Has Abstractpub_date
2015-02-01 00:00:00pages
178-85issue
2eissn
2157-6564issn
2157-6580pii
sctm.2014-0103journal_volume
4pub_type
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pub_type: 杂志文章,多中心研究
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2013-0035
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2013-0084
更新日期:2014-01-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2015-0264
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journal_title:Stem cells translational medicine
pub_type: 临床试验,杂志文章
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
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journal_title:Stem cells translational medicine
pub_type: 临床试验,杂志文章
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更新日期:2015-12-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
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更新日期:2016-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2014-0200
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
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更新日期:2013-02-01 00:00:00
abstract::The method of isolation of bone marrow (BM) mesenchymal stem/stromal cells (MSCs) is a limiting factor in their study and therapeutic use. MSCs are typically expanded from BM cells selected on the basis of their adherence to plastic, which results in a heterogeneous population of cells. Prospective identification of t...
journal_title:Stem cells translational medicine
pub_type: 杂志文章
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