当前位置： SCI文献检索 > Stem Cells Translational Medicine期刊下所有文献 > Lung Injury Repair by Transplantation of Adult Lung Cells Following Preconditioning of Recipient Mice.

Lung Injury Repair by Transplantation of Adult Lung Cells Following Preconditioning of Recipient Mice.

Abstract：

:Repair of injured lungs represents a longstanding therapeutic challenge. We recently demonstrated that human and mouse embryonic lung tissue from the canalicular stage of development are enriched with lung progenitors, and that a single cell suspension of canalicular lungs can be used for transplantation, provided that lung progenitor niches in the recipient mice are vacated by strategies similar to those used in bone marrow transplantation. Considering the ethical limitations associated with the use of fetal cells, we investigated here whether adult lungs could offer an alternative source of lung progenitors for transplantation. We show that intravenous infusion of a single cell suspension of adult mouse lungs from GFP+ donors, following conditioning of recipient mice with naphthalene and subsequent sublethal irradiation, led to marked colonization of the recipient lungs, at 6-8 weeks post-transplant, with donor derived structures including epithelial, endothelial, and mesenchymal cells. Epithelial cells within these donor-derived colonies expressed markers of functionally distinct lung cell types, and lung function, which is significantly compromised in mice treated with naphthalene and radiation, was found to be corrected following transplantation. Dose response analysis suggests that the frequency of patch forming cells in adult lungs was about threefold lower compared to that found in E16 fetal lungs. However, as adult lungs are much larger, the total number of patch forming cells that can be collected from this source is significantly greater. Our study provides proof of concept for lung regeneration by adult lung cells after preconditioning to vacate the pulmonary niche. Stem Cells Translational Medicine 2018;7:68-77.

journal_name

Stem Cells Transl Med

journal_title

Stem cells translational medicine

authors

Milman Krentsis I,Rosen C,Shezen E,Aronovich A,Nathanson B,Bachar-Lustig E,Berkman N,Assayag M,Shakhar G,Feferman T,Orgad R,Reisner Y

doi

10.1002/sctm.17-0149

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

68-77

issue

eissn

2157-6564

issn

2157-6580

journal_volume

pub_type

杂志文章

在线工具

Lung Injury Repair by Transplantation of Adult Lung Cells Following Preconditioning of Recipient Mice.