Pat1 promotes processing body assembly by enhancing the phase separation of the DEAD-box ATPase Dhh1 and RNA.

abstract：:DNA is a remarkably precise medium for copying and storing biological information. This high fidelity results from the action of hundreds of genes involved in replication, proofreading, and damage repair. Evolutionary theory suggests that in such a system, selection has limited ability to remove genetic variants that ...

journal_title：eLife

authors： Harris K,Pritchard JK

更新日期：2017-04-25 00:00:00

abstract：:The production of action sequences is a fundamental aspect of motor skills. To examine whether primary motor cortex (M1) is involved in maintenance of sequential movements, we trained two monkeys (Cebus apella) to perform two sequential reaching tasks. In one task, sequential movements were instructed by visual cues, ...

journal_title：eLife

authors： Ohbayashi M

更新日期：2020-02-10 00:00:00

abstract：:A polymorphism in the autophagy gene Atg16l1 is associated with susceptibility to inflammatory bowel disease (IBD); however, it remains unclear how autophagy contributes to intestinal immune homeostasis. Here, we demonstrate that autophagy is essential for maintenance of balanced CD4(+) T cell responses in the intesti...

journal_title：eLife

authors： Kabat AM,Harrison OJ,Riffelmacher T,Moghaddam AE,Pearson CF,Laing A,Abeler-Dörner L,Forman SP,Grencis RK,Sattentau Q,Simon AK,Pott J,Maloy KJ

更新日期：2016-02-24 00:00:00

abstract：:The roles of long non-coding RNAs (lncRNAs) in regulating cancer and stem cells are being increasingly appreciated. Its diverse mechanisms provide the regulatory network with a bigger repertoire to increase complexity. Here we report a novel LncRNA, Lnc34a, that is enriched in colon cancer stem cells (CCSCs) and initi...

journal_title：eLife

authors： Wang L,Bu P,Ai Y,Srinivasan T,Chen HJ,Xiang K,Lipkin SM,Shen X

更新日期：2016-04-14 00:00:00

abstract：:When an individual makes a judgement about the actions of another individual, taking the latter's viewpoint into consideration enhances cooperation in society at large. ...

journal_title：eLife

authors： Masuda N,Santos FC

更新日期：2019-04-29 00:00:00

abstract：:Patterns of spatial positioning of individuals within microbial communities are often critical to community function. However, understanding patterning in natural communities is hampered by the multitude of cell-cell and cell-environment interactions as well as environmental variability. Here, through simulations and ...

journal_title：eLife

authors： Momeni B,Brileya KA,Fields MW,Shou W

更新日期：2013-01-22 00:00:00

abstract：:Hepcidin is the master regulator of systemic iron homeostasis. Derived primarily from the liver, it inhibits the iron exporter ferroportin in the gut and spleen, the sites of iron absorption and recycling respectively. Recently, we demonstrated that ferroportin is also found in cardiomyocytes, and that its cardiac-spe...

journal_title：eLife

authors： Lakhal-Littleton S,Wolna M,Chung YJ,Christian HC,Heather LC,Brescia M,Ball V,Diaz R,Santos A,Biggs D,Clarke K,Davies B,Robbins PA

更新日期：2016-11-29 00:00:00

abstract：:Signaling by the G-protein-coupled receptors (GPCRs) plays fundamental role in a vast number of essential physiological functions. Precise control of GPCR signaling requires action of regulators of G protein signaling (RGS) proteins that deactivate heterotrimeric G proteins. RGS proteins are elaborately regulated and ...

journal_title：eLife

authors： Patil DN,Rangarajan ES,Novick SJ,Pascal BD,Kojetin DJ,Griffin PR,Izard T,Martemyanov KA

更新日期：2018-12-12 00:00:00

abstract：:Two different cell signals often affect transcription of the same gene. In such cases, it is natural to ask how the combined transcriptional response compares to the individual responses. The most commonly used mechanistic models predict additive or multiplicative combined responses, but a systematic genome-wide evalu...

journal_title：eLife

authors： Sanford EM,Emert BL,Coté A,Raj A

更新日期：2020-12-07 00:00:00

abstract：:Genome-wide association analyses have uncovered multiple genomic regions associated with T2D, but identification of the causal variants at these remains a challenge. There is growing interest in the potential of deep learning models - which predict epigenome features from DNA sequence - to support inference concerning...

journal_title：eLife

authors： Wesolowska-Andersen A,Zhuo Yu G,Nylander V,Abaitua F,Thurner M,Torres JM,Mahajan A,Gloyn AL,McCarthy MI

更新日期：2020-01-27 00:00:00

abstract：:To initiate DNA replication, cells first load an MCM helicase double hexamer at origins in a reaction requiring ORC, Cdc6, and Cdt1, also called pre-replicative complex (pre-RC) assembly. The essential mechanistic role of Cdc6 ATP hydrolysis in this reaction is still incompletely understood. Here, we show that althoug...

journal_title：eLife

authors： Chang F,Riera A,Evrin C,Sun J,Li H,Speck C,Weinreich M

更新日期：2015-08-25 00:00:00

abstract：:Accurate protein localization is crucial to generate and maintain organization in all cells. Achieving accuracy is challenging, as the molecular signals that dictate a protein's cellular destination are often promiscuous. A salient example is the targeting of an essential class of tail-anchored (TA) proteins, whose so...

journal_title：eLife

authors： Rao M,Okreglak V,Chio US,Cho H,Walter P,Shan SO

更新日期：2016-12-07 00:00:00

abstract：:Perturbation of neuronal activity is key to understanding the brain's functional properties, however, intervention studies typically perturb neurons in a nonspecific manner. Recent optogenetics techniques have enabled patterned perturbations, in which specific patterns of activity can be invoked in identified target n...

journal_title：eLife

authors： Sadeh S,Clopath C

更新日期：2020-02-19 00:00:00

abstract：:Anterograde transport of late endosomes or lysosomes (LE/Lys) is crucial for proper axon growth. However, the role of energetic nutrients has been poorly explored. Malonyl-CoA is a precursor of fatty acids, and its intracellular levels highly fluctuate depending on glucose availability or the energy sensor AMP-activat...

journal_title：eLife

authors： Palomo-Guerrero M,Fadó R,Casas M,Pérez-Montero M,Baena M,Helmer PO,Domínguez JL,Roig A,Serra D,Hayen H,Stenmark H,Raiborg C,Casals N

更新日期：2019-12-23 00:00:00

abstract：:Previous studies tracking AMPA receptor (AMPAR) diffusion at synapses observed a large mobile extrasynaptic AMPAR pool. Using super-resolution microscopy, we examined how fluorophore size and photostability affected AMPAR trafficking outside of, and within, post-synaptic densities (PSDs) from rats. Organic fluorescent...

journal_title：eLife

authors： Lee SH,Jin C,Cai E,Ge P,Ishitsuka Y,Teng KW,de Thomaz AA,Nall D,Baday M,Jeyifous O,Demonte D,Dundas CM,Park S,Delgado JY,Green WN,Selvin PR

更新日期：2017-07-27 00:00:00

abstract：:KdpFABC is an ATP-dependent K+ pump that ensures bacterial survival in K+-deficient environments. Whereas transcriptional activation of kdpFABC expression is well studied, a mechanism for down-regulation when K+ levels are restored has not been described. Here, we show that KdpFABC is inhibited when cells return to a ...

journal_title：eLife

authors： Sweet ME,Zhang X,Erdjument-Bromage H,Dubey V,Khandelia H,Neubert TA,Pedersen BP,Stokes DL

更新日期：2020-09-21 00:00:00

abstract：:Unrepaired DNA damage during embryonic development can be potentially inherited by a large population of cells. However, the quality control mechanisms that minimize the contribution of damaged cells to developing embryos remain poorly understood. Here, we uncovered an ATR- and CHK1-mediated transcriptional response t...

journal_title：eLife

authors： Atashpaz S,Samadi Shams S,Gonzalez JM,Sebestyén E,Arghavanifard N,Gnocchi A,Albers E,Minardi S,Faga G,Soffientini P,Allievi E,Cancila V,Bachi A,Fernández-Capetillo Ó,Tripodo C,Ferrari F,López-Contreras AJ,Costanzo V

更新日期：2020-03-12 00:00:00

abstract：:Metabolic cycles are a fundamental element of cellular and organismal function. Among the most critical in higher organisms is the Cori Cycle, the systemic cycling between lactate and glucose. Here, skeletal muscle-specific Mitochondrial Pyruvate Carrier (MPC) deletion in mice diverted pyruvate into circulating lactat...

journal_title：eLife

authors： Sharma A,Oonthonpan L,Sheldon RD,Rauckhorst AJ,Zhu Z,Tompkins SC,Cho K,Grzesik WJ,Gray LR,Scerbo DA,Pewa AD,Cushing EM,Dyle MC,Cox JE,Adams C,Davies BS,Shields RK,Norris AW,Patti G,Zingman LV,Taylor EB

更新日期：2019-07-18 00:00:00

abstract：:Humans can run without falling down, usually despite uneven terrain or occasional pushes. Even without such external perturbations, intrinsic sources like sensorimotor noise perturb the running motion incessantly, making each step variable. Here, using simple and generalizable models, we show that even such small step...

journal_title：eLife

authors： Seethapathi N,Srinivasan M

更新日期：2019-03-19 00:00:00

abstract：:In homeostatic scaling at central synapses, the depth and breadth of cellular mechanisms that detect the offset from the set-point, detect the duration of the offset and implement a cellular response are not well understood. To understand the time-dependent scaling dynamics we treated cultured rat hippocampal cells wi...

journal_title：eLife

authors： Schanzenbächer CT,Langer JD,Schuman EM

更新日期：2018-02-15 00:00:00

abstract：:Swi2/Snf2 ATPases remodel substrates such as nucleosomes and transcription complexes to control a wide range of DNA-associated processes, but detailed structural information on the ATP-dependent remodeling reactions is largely absent. The single subunit remodeler Mot1 (modifier of transcription 1) dissociates TATA box...

journal_title：eLife

authors： Butryn A,Schuller JM,Stoehr G,Runge-Wollmann P,Förster F,Auble DT,Hopfner KP

更新日期：2015-08-10 00:00:00

abstract：:The integration of cellular and molecular structural data is key to understanding the function of macromolecular assemblies and complexes in their in vivo context. Here we report on the outcomes of a workshop that discussed how to integrate structural data from a range of public archives. The workshop identified two m...

journal_title：eLife

authors： Patwardhan A,Brandt R,Butcher SJ,Collinson L,Gault D,Grünewald K,Hecksel C,Huiskonen JT,Iudin A,Jones ML,Korir PK,Koster AJ,Lagerstedt I,Lawson CL,Mastronarde D,McCormick M,Parkinson H,Rosenthal PB,Saalfeld S,Saibil

更新日期：2017-07-06 00:00:00

abstract：:Sex-optimal diets have different effects on gene expression in female and male flies. ...

journal_title：eLife

authors： Flatt T

更新日期：2019-10-01 00:00:00

abstract：:Mouse GnT1IP-L, and membrane-bound GnT1IP-S (MGAT4D) expressed in cultured cells inhibit MGAT1, the N-acetylglucosaminyltransferase that initiates the synthesis of hybrid and complex N-glycans. However, it is not known where in the secretory pathway GnT1IP-L inhibits MGAT1, nor whether GnT1IP-L inhibits other N-glycan...

journal_title：eLife

authors： Huang HH,Hassinen A,Sundaram S,Spiess AN,Kellokumpu S,Stanley P

更新日期：2015-09-15 00:00:00

abstract：:Dedifferentiation of acini to duct-like cells occurs during the physiologic damage response in the pancreas, but this process can be co-opted by oncogenic Kras to drive carcinogenesis. Myeloid cells infiltrate the pancreas during the onset of pancreatic cancer, and promote carcinogenesis. Here, we show that the functi...

journal_title：eLife

authors： Zhang Y,Yan W,Mathew E,Kane KT,Brannon A 3rd,Adoumie M,Vinta A,Crawford HC,Pasca di Magliano M

更新日期：2017-10-05 00:00:00

abstract：:C-type inactivation of potassium channels fine-tunes the electrical signaling in excitable cells through an internal timing mechanism that is mediated by a hydrogen bond network in the channels' selectively filter. Previously, we used nonsense suppression to highlight the role of the conserved Trp434-Asp447 indole hyd...

journal_title：eLife

authors： Lueck JD,Mackey AL,Infield DT,Galpin JD,Li J,Roux B,Ahern CA

更新日期：2016-10-06 00:00:00

abstract：:Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of Saccharomyces cerevisiae 90S determined from three cryo-electron microscopy single particle...

journal_title：eLife

authors： Sun Q,Zhu X,Qi J,An W,Lan P,Tan D,Chen R,Wang B,Zheng S,Zhang C,Chen X,Zhang W,Chen J,Dong MQ,Ye K

更新日期：2017-02-28 00:00:00

abstract：:SynGAP is a postsynaptic density (PSD) protein that binds to PDZ domains of the scaffold protein PSD-95. We previously reported that heterozygous deletion of Syngap1 in mice is correlated with increased steady-state levels of other key PSD proteins that bind PSD-95, although the level of PSD-95 remains constant (Walku...

journal_title：eLife

authors： Mastro TL,Preza A,Basu S,Chattarji S,Till SM,Kind PC,Kennedy MB

更新日期：2020-01-15 00:00:00

abstract：:Interactions between membrane protein interfaces in lipid bilayers play an important role in membrane protein folding but quantification of the strength of these interactions has been challenging. Studying dimerization of ClC-type transporters offers a new approach to the problem, as individual subunits adopt a stable...

journal_title：eLife

authors： Chadda R,Krishnamani V,Mersch K,Wong J,Brimberry M,Chadda A,Kolmakova-Partensky L,Friedman LJ,Gelles J,Robertson JL

更新日期：2016-08-03 00:00:00

abstract：:A spring-loaded mechanism can explain the activation process for a protein that has a crucial role in maintaining the genomic integrity of immature eggs cells. ...

journal_title：eLife

authors： Amelio I,Melino G

更新日期：2016-05-23 00:00:00