Super-resolution imaging of synaptic and Extra-synaptic AMPA receptors with different-sized fluorescent probes.

abstract：:Recent findings indicate a high level of specialization at the level of microcircuits and cell populations within brain structures with regards to the control of fear and anxiety. The hippocampus, however, has been treated as a unitary structure in anxiety and fear research despite mounting evidence that different hip...

journal_title：eLife

authors： Engin E,Smith KS,Gao Y,Nagy D,Foster RA,Tsvetkov E,Keist R,Crestani F,Fritschy JM,Bolshakov VY,Hajos M,Heldt SA,Rudolph U

更新日期：2016-03-14 00:00:00

abstract：:The twin-arginine protein translocation system (Tat) transports folded proteins across the bacterial cytoplasmic membrane and the thylakoid membranes of plant chloroplasts. The Tat transporter is assembled from multiple copies of the membrane proteins TatA, TatB, and TatC. We combine sequence co-evolution analysis, mo...

journal_title：eLife

authors： Alcock F,Stansfeld PJ,Basit H,Habersetzer J,Baker MA,Palmer T,Wallace MI,Berks BC

更新日期：2016-12-03 00:00:00

abstract：:Multiple studies have reported a male bias in incidence and/or prevalence of malaria infection in males compared to females. To test the hypothesis that sex-based differences in host-parasite interactions affect the epidemiology of malaria, we intensively followed Plasmodium falciparum infections in a cohort in a mala...

journal_title：eLife

authors： Briggs J,Teyssier N,Nankabirwa JI,Rek J,Jagannathan P,Arinaitwe E,Bousema T,Drakeley C,Murray M,Crawford E,Hathaway N,Staedke SG,Smith D,Rosenthal PJ,Kamya M,Dorsey G,Rodriguez-Barraquer I,Greenhouse B

更新日期：2020-10-27 00:00:00

abstract：:Most funding agencies rely on peer review to evaluate grant applications and proposals, but research into the use of this process by funding agencies has been limited. Here we explore if two changes to the organization of peer review for proposals submitted to various funding actions by the European Union has an influ...

journal_title：eLife

authors： Pina DG,Buljan I,Hren D,Marušić A

更新日期：2021-01-13 00:00:00

abstract：:Iron and heme play central roles in the production of red blood cells, but the underlying mechanisms remain incompletely understood. Heme-regulated eIF2α kinase (HRI) controls translation by phosphorylating eIF2α. Here, we investigate the global impact of iron, heme, and HRI on protein translation in vivo in murine pr...

journal_title：eLife

authors： Zhang S,Macias-Garcia A,Ulirsch JC,Velazquez J,Butty VL,Levine SS,Sankaran VG,Chen JJ

更新日期：2019-04-29 00:00:00

abstract：:Although individuals of many species inexorably age, a number of observations established that the rate of aging is modulated in response to a variety of mild stresses. Here, we investigated how heat stress promotes longevity in yeast. We show that upon growth at higher temperature, yeast cells relax the retention of ...

journal_title：eLife

authors： Baldi S,Bolognesi A,Meinema AC,Barral Y

更新日期：2017-12-28 00:00:00

abstract：:As the equipment needed to perform state-of-the-art research in many areas of biology becomes ever more expensive, Eve Marder worries that researchers in less wealthy institutions might be left behind. ...

journal_title：eLife

authors： Marder E

更新日期：2013-11-19 00:00:00

abstract：:Bioluminescence is a powerful biological signal that scientists have repurposed as a reporter for gene expression in plants and animals. However, there are downsides associated with the need to provide a substrate to these reporters, including its high cost and non-uniform tissue penetration. In this work we reconstit...

journal_title：eLife

authors： Khakhar A,Starker CG,Chamness JC,Lee N,Stokke S,Wang C,Swanson R,Rizvi F,Imaizumi T,Voytas DF

更新日期：2020-03-25 00:00:00

abstract：:Cytomegaloviruses (CMVs) are ubiquitous pathogens known to employ numerous immunoevasive strategies that significantly impair the ability of the immune system to eliminate the infected cells. Here, we report that the single mouse CMV (MCMV) protein, m154, downregulates multiple surface molecules involved in the activa...

journal_title：eLife

authors： Strazic Geljic I,Kucan Brlic P,Angulo G,Brizic I,Lisnic B,Jenus T,Juranic Lisnic V,Pietri GP,Engel P,Kaynan N,Zeleznjak J,Schu P,Mandelboim O,Krmpotic A,Angulo A,Jonjic S,Lenac Rovis T

更新日期：2020-01-13 00:00:00

abstract：:Studies of cell fate focus on specification, but little is known about maintenance of the differentiated state. In this study, we find that the mouse tendon cell fate requires continuous maintenance in vivo and identify an essential role for TGFβ signaling in maintenance of the tendon cell fate. To examine the role of...

journal_title：eLife

authors： Tan GK,Pryce BA,Stabio A,Brigande JV,Wang C,Xia Z,Tufa SF,Keene DR,Schweitzer R

更新日期：2020-01-21 00:00:00

abstract：:The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed 'Opto-CRAC') that selectively ...

journal_title：eLife

authors： He L,Zhang Y,Ma G,Tan P,Li Z,Zang S,Wu X,Jing J,Fang S,Zhou L,Wang Y,Huang Y,Hogan PG,Han G,Zhou Y

更新日期：2015-12-08 00:00:00

abstract：:Cell-cell communication in multicellular organisms depends on the dynamic and reversible phosphorylation of protein tyrosine residues. The receptor-linked protein tyrosine phosphatases (RPTPs) receive cues from the extracellular environment and are well placed to influence cell signaling. However, the direct events do...

journal_title：eLife

authors： Fearnley GW,Young KA,Edgar JR,Antrobus R,Hay IM,Liang WC,Martinez-Martin N,Lin W,Deane JE,Sharpe HJ

更新日期：2019-03-29 00:00:00

abstract：:Massed synchronised neuronal firing is detrimental to information processing. When networks of task-irrelevant neurons fire in unison, they mask the signal generated by task-critical neurons. On a macroscopic level, such synchronisation can contribute to alpha/beta (8-30 Hz) oscillations. Reducing the amplitude of the...

journal_title：eLife

authors： Griffiths BJ,Mayhew SD,Mullinger KJ,Jorge J,Charest I,Wimber M,Hanslmayr S

更新日期：2019-11-29 00:00:00

abstract：:A hallmark of CRISPR-Cas immunity systems is the CRISPR array, a genomic locus consisting of short, repeated sequences ('repeats') interspersed with short, variable sequences ('spacers'). CRISPR arrays are transcribed and processed into individual CRISPR RNAs that each include a single spacer, and direct Cas proteins ...

journal_title：eLife

authors： Stringer AM,Baniulyte G,Lasek-Nesselquist E,Seed KD,Wade JT

更新日期：2020-10-30 00:00:00

abstract：:Excitatory and inhibitory synapses are the brain's most abundant synapse types. However, little is known about their formation during critical periods of motor skill learning, when sensory experience defines a motor target that animals strive to imitate. In songbirds, we find that exposure to tutor song leads to elimi...

journal_title：eLife

authors： Huang Z,Khaled HG,Kirschmann M,Gobes SM,Hahnloser RH

更新日期：2018-10-25 00:00:00

abstract：:Although it is clear that trisomy 21 causes Down syndrome, the molecular events acting downstream of the trisomy remain ill defined. Using complementary genomics analyses, we identified the interferon pathway as the major signaling cascade consistently activated by trisomy 21 in human cells. Transcriptome analysis rev...

journal_title：eLife

authors： Sullivan KD,Lewis HC,Hill AA,Pandey A,Jackson LP,Cabral JM,Smith KP,Liggett LA,Gomez EB,Galbraith MD,DeGregori J,Espinosa JM

更新日期：2016-07-29 00:00:00

abstract：:New data on the relationship between two proteins, cyclin D and Rb, suggest that we need to re-evaluate our understanding of how cells enter into the cell cycle. ...

journal_title：eLife

authors： Bertoli C,de Bruin RA

更新日期：2014-07-01 00:00:00

abstract：:Even when we are at rest, our spinal cords show spontaneous, yet well organised, fluctuations of activity that might reflect sensory and motor networks. ...

journal_title：eLife

authors： Eippert F,Tracey I

更新日期：2014-08-05 00:00:00

abstract：:Periodontal disease is an age-associated disorder clinically defined by periodontal bone loss, inflammation of the specialized tissues that surround and support the tooth, and microbiome dysbiosis. Currently, there is no therapy for reversing periodontal disease, and treatment is generally restricted to preventive mea...

journal_title：eLife

authors： An JY,Kerns KA,Ouellette A,Robinson L,Morris HD,Kaczorowski C,Park SI,Mekvanich T,Kang A,McLean JS,Cox TC,Kaeberlein M

更新日期：2020-04-28 00:00:00

abstract：:In hypoxic stress conditions, glycolysis enzymes assemble into singular cytoplasmic granules called glycolytic (G) bodies. G body formation in yeast correlates with increased glucose consumption and cell survival. However, the physical properties and organizing principles that define G body formation are unclear. We d...

journal_title：eLife

authors： Fuller GG,Han T,Freeberg MA,Moresco JJ,Ghanbari Niaki A,Roach NP,Yates JR 3rd,Myong S,Kim JK

更新日期：2020-04-16 00:00:00

abstract：:Bone destruction relies on interactions between bone and immune cells. Bone-resorbing osteoclasts (OCLs) were recently identified as innate immune cells activating T cells toward tolerance or inflammation. Thus, pathological bone destruction not only relies on increased osteoclast differentiation, but also on the pres...

journal_title：eLife

authors： Madel MB,Ibáñez L,Ciucci T,Halper J,Rouleau M,Boutin A,Hue C,Duroux-Richard I,Apparailly F,Garchon HJ,Wakkach A,Blin-Wakkach C

更新日期：2020-05-13 00:00:00

abstract：:Knowledge of the host factors required for norovirus replication has been hindered by the challenges associated with culturing human noroviruses. We have combined proteomic analysis of the viral translation and replication complexes with a CRISPR screen, to identify host factors required for norovirus infection. The c...

journal_title：eLife

authors： Hosmillo M,Lu J,McAllaster MR,Eaglesham JB,Wang X,Emmott E,Domingues P,Chaudhry Y,Fitzmaurice TJ,Tung MK,Panas MD,McInerney G,Locker N,Wilen CB,Goodfellow IG

更新日期：2019-08-12 00:00:00

abstract：:Perforin-2 (MPEG1) is a pore-forming, antibacterial protein with broad-spectrum activity. Perforin-2 is expressed constitutively in phagocytes and inducibly in parenchymal, tissue-forming cells. In vitro, Perforin-2 prevents the intracellular replication and proliferation of bacterial pathogens in these cells. Perfori...

journal_title：eLife

authors： McCormack RM,de Armas LR,Shiratsuchi M,Fiorentino DG,Olsson ML,Lichtenheld MG,Morales A,Lyapichev K,Gonzalez LE,Strbo N,Sukumar N,Stojadinovic O,Plano GV,Munson GP,Tomic-Canic M,Kirsner RS,Russell DG,Podack ER

更新日期：2015-09-24 00:00:00

abstract：:Cell lineages provide the framework for understanding how cell fates are decided during development. Describing cell lineages in most organisms is challenging; even a fruit fly larva has ~50,000 cells and a small mammal has >1 billion cells. Recently, the idea of applying CRISPR to induce mutations during development,...

journal_title：eLife

authors： Salvador-Martínez I,Grillo M,Averof M,Telford MJ

更新日期：2019-01-28 00:00:00

abstract：:The emergence of subpopulations that perform distinct metabolic roles has been observed in populations of genetically identical bacteria. ...

journal_title：eLife

authors： Dal Co A,Brannon C,Ackermann M

更新日期：2018-06-29 00:00:00

abstract：:Whether neurons encode information through their spike rates, their activity times or both is an ongoing debate in systems neuroscience. Here, we tested whether humans can discriminate between a pair of temporal odor mixtures (TOMs) composed of the same two components delivered in rapid succession in either one tempor...

journal_title：eLife

authors： Perl O,Nahum N,Belelovsky K,Haddad R

更新日期：2020-02-07 00:00:00

abstract：:Bacterial cells are encased in a peptidoglycan (PG) exoskeleton that protects them from osmotic lysis and specifies their distinct shapes. Cell wall hydrolases are required to enlarge this covalently closed macromolecule during growth, but how these autolytic enzymes are regulated remains poorly understood. Bacillus s...

journal_title：eLife

authors： Dobihal GS,Brunet YR,Flores-Kim J,Rudner DZ

更新日期：2019-12-06 00:00:00

abstract：:Pervasive expression of circular RNA is a recently discovered feature of eukaryotic gene expression programs, yet its function remains largely unknown. The presumed biogenesis of these RNAs involves a non-canonical 'backsplicing' event. Recent studies in mammalian cell culture posit that backsplicing is facilitated by...

journal_title：eLife

authors： Barrett SP,Wang PL,Salzman J

更新日期：2015-06-09 00:00:00

abstract：:Photosynthetic starch reserves that accumulate in Arabidopsis leaves during the day decrease approximately linearly with time at night to support metabolism and growth. We find that the rate of decrease is adjusted to accommodate variation in the time of onset of darkness and starch content, such that reserves last al...

journal_title：eLife

authors： Scialdone A,Mugford ST,Feike D,Skeffington A,Borrill P,Graf A,Smith AM,Howard M

更新日期：2013-06-25 00:00:00

abstract：:We analyzed 2898 scientific papers published between 1995 and 2017 in which two or more authors shared the first author position. For papers in which the first and second authors made equal contributions, mixed-gender combinations were most frequent, followed by male-male and then female-female author combinations. Fo...

journal_title：eLife

authors： Broderick NA,Casadevall A

更新日期：2019-01-29 00:00:00