Abstract:
:The integration of cellular and molecular structural data is key to understanding the function of macromolecular assemblies and complexes in their in vivo context. Here we report on the outcomes of a workshop that discussed how to integrate structural data from a range of public archives. The workshop identified two main priorities: the development of tools and file formats to support segmentation (that is, the decomposition of a three-dimensional volume into regions that can be associated with defined objects), and the development of tools to support the annotation of biological structures.
journal_name
Elifejournal_title
eLifeauthors
Patwardhan A,Brandt R,Butcher SJ,Collinson L,Gault D,Grünewald K,Hecksel C,Huiskonen JT,Iudin A,Jones ML,Korir PK,Koster AJ,Lagerstedt I,Lawson CL,Mastronarde D,McCormick M,Parkinson H,Rosenthal PB,Saalfeld S,Saibildoi
10.7554/eLife.25835subject
Has Abstractpub_date
2017-07-06 00:00:00issn
2050-084Xjournal_volume
6pub_type
相关文献
eLife文献大全abstract::Many organisms lose the capacity to regenerate damaged tissues as they mature. Damaged Drosophila imaginal discs regenerate efficiently early in the third larval instar (L3) but progressively lose this ability. This correlates with reduced damage-responsive expression of multiple genes, including the WNT genes wingles...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.11588
更新日期:2016-02-03 00:00:00
abstract::Recent studies suggested an essential role for seryl-tRNA synthetase (SerRS) in vascular development. This role is specific to SerRS among all tRNA synthetases and is independent of its well-known aminoacylation function in protein synthesis. A unique nucleus-directing domain, added at the invertebrate-to-vertebrate t...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.02349
更新日期:2014-06-17 00:00:00
abstract::The transcription factor TCF7L1 is an embryonic stem cell signature gene that is upregulated in multiple aggressive cancer types, but its role in skin tumorigenesis has not yet been defined. Here we document TCF7L1 upregulation in skin squamous cell carcinoma (SCC) and demonstrate that TCF7L1 overexpression increases ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23242
更新日期:2017-05-03 00:00:00
abstract::A transcription factor that regulates skeleton formation in sea urchin embryos has evolved a new domain that is essential for this process. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.33792
更新日期:2018-01-08 00:00:00
abstract::Acquisition of pluripotency is driven largely at the transcriptional level by activators OCT4, SOX2, and NANOG that must in turn cooperate with diverse coactivators to execute stem cell-specific gene expression programs. Using a biochemically defined in vitro transcription system that mediates OCT4/SOX2 and coactivato...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03573
更新日期:2014-11-19 00:00:00
abstract::It is widely argued that personalized instruction based on individual differences in learning styles or genetic predispositions could improve learning outcomes. However, this proposition has resisted clear demonstration in human studies, where it is difficult to control experience and quantify outcomes. Here, we take ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47216
更新日期:2019-09-17 00:00:00
abstract::To understand the neural origins of rhythmic behavior one must characterize the central pattern generator circuit and quantify the population size needed to sustain functionality. Breathing-related interneurons of the brainstem pre-Bötzinger complex (preBötC) that putatively comprise the core respiratory rhythm genera...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03427
更新日期:2014-07-15 00:00:00
abstract::Planar cell polarity (PCP) requires the asymmetric sorting of distinct signaling receptors to distal and proximal surfaces of polarized epithelial cells. We have examined the transport of one PCP signaling protein, Vangl2, from the trans Golgi network (TGN) in mammalian cells. Using siRNA knockdown experiments, we fin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00160
更新日期:2013-01-08 00:00:00
abstract::Positive-strand RNA viruses, the largest genetic class of viruses, include numerous important pathogens such as Zika virus. These viruses replicate their RNA genomes in novel, membrane-bounded mini-organelles, but the organization of viral proteins and RNAs in these compartments has been largely unknown. We used cryo-...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25940
更新日期:2017-06-27 00:00:00
abstract::Accurate chromosome segregation relies on bioriented amphitelic attachments of chromosomes to microtubules of the mitotic spindle, in which sister chromatids are connected to opposite spindle poles. BUB-1 is a protein of the Spindle Assembly Checkpoint (SAC) that coordinates chromosome attachment with anaphase onset. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.40690
更新日期:2018-12-14 00:00:00
abstract::Two different cell signals often affect transcription of the same gene. In such cases, it is natural to ask how the combined transcriptional response compares to the individual responses. The most commonly used mechanistic models predict additive or multiplicative combined responses, but a systematic genome-wide evalu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59388
更新日期:2020-12-07 00:00:00
abstract::Cell-type-specific 3D organization of the genome is unrecognizable during mitosis. It remains unclear how essential positional information is transmitted through cell division such that a daughter cell recapitulates the spatial genome organization of the parent. Lamina-associated domains (LADs) are regions of repressi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49278
更新日期:2019-10-01 00:00:00
abstract::Mammalian lifespan differs by >100 fold, but the mechanisms associated with such longevity differences are not understood. Here, we conducted a study on primary skin fibroblasts isolated from 16 species of mammals and maintained under identical cell culture conditions. We developed a pipeline for obtaining species-spe...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19130
更新日期:2016-11-22 00:00:00
abstract::Recent findings indicate a high level of specialization at the level of microcircuits and cell populations within brain structures with regards to the control of fear and anxiety. The hippocampus, however, has been treated as a unitary structure in anxiety and fear research despite mounting evidence that different hip...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14120
更新日期:2016-03-14 00:00:00
abstract::Adhesion molecules hold cells together but also couple cell membranes to a contractile actomyosin network, which limits the expansion of cell contacts. Despite their fundamental role in tissue morphogenesis and tissue homeostasis, how adhesion molecules control cell shapes and cell patterns in tissues remains unclear....
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22796
更新日期:2017-05-24 00:00:00
abstract::Compartmentalized oscillations of second messengers affect global cellular signaling. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.63845
更新日期:2020-11-17 00:00:00
abstract::New ages for flowstone, sediments and fossil bones from the Dinaledi Chamber are presented. We combined optically stimulated luminescence dating of sediments with U-Th and palaeomagnetic analyses of flowstones to establish that all sediments containing Homo naledi fossils can be allocated to a single stratigraphic ent...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.24231
更新日期:2017-05-09 00:00:00
abstract::RNA-protein (RNP) granules have been proposed to assemble by forming solid RNA/protein aggregates or through phase separation into a liquid RNA/protein phase. Which model describes RNP granules in living cells is still unclear. In this study, we analyze P bodies in budding yeast and find that they have liquid-like pro...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.06807
更新日期:2015-08-04 00:00:00
abstract::The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12470
更新日期:2016-03-01 00:00:00
abstract::Upon detecting endoplasmic reticulum (ER) stress, the unfolded protein response (UPR) orchestrates adaptive cellular changes to reestablish homeostasis. If stress resolution fails, the UPR commits the cell to apoptotic death. Here we show that in hematopoietic cells, including multiple myeloma (MM), lymphoma, and leuk...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47084
更新日期:2019-08-27 00:00:00
abstract::Cyanobacteria were responsible for the oxygenation of the ancient atmosphere; however, the evolution of this phylum is enigmatic, as relatives have not been characterized. Here we use whole genome reconstruction of human fecal and subsurface aquifer metagenomic samples to obtain complete genomes for members of a new c...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01102
更新日期:2013-10-01 00:00:00
abstract::Amyloid plaques, consisting of deposited beta-amyloid (Aβ), are a neuropathological hallmark of Alzheimer's Disease (AD). Cerebral vessels play a major role in AD, as Aβ is cleared from the brain by pathways involving the cerebrovasculature, most AD patients have cerebrovascular amyloid (cerebral amyloid angiopathy (C...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.29595
更新日期:2017-10-10 00:00:00
abstract::Our understanding of nonlinear stimulus transformations by neural circuits is hindered by the lack of comprehensive yet interpretable computational modeling frameworks. Here, we propose a data-driven approach based on deep neural networks to directly model arbitrarily nonlinear stimulus-response mappings. Reformulatin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53445
更新日期:2020-06-26 00:00:00
abstract::Drug treatment of 3D cancer spheroids more accurately reflects in vivo therapeutic responses compared to adherent culture studies. In EGFR-mutated lung adenocarcinoma, EGFR-TKIs show enhanced efficacy in spheroid cultures. Simultaneous inhibition of multiple parallel RTKs further enhances EGFR-TKI effectiveness. We sh...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58204
更新日期:2020-09-08 00:00:00
abstract::The prefrontal cortex (PFC)'s functions are thought to include working memory, as its activity can reflect information that must be temporarily maintained to realize the current goal. We designed a flexible spatial working memory task that required rats to navigate - after distractions and a delay - to multiple possib...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.63035
更新日期:2020-12-24 00:00:00
abstract::Kinesin super family protein 2A (KIF2A), an ATP-dependent microtubule (MT) destabilizer, regulates cell migration, axon elongation, and pruning in the developing nervous system. KIF2A mutations have recently been identified in patients with malformed cortical development. However, postnatal KIF2A is continuously expre...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30935
更新日期:2018-01-09 00:00:00
abstract::Microtubules are nucleated from specific locations at precise times in the cell cycle. However, the factors that constitute these microtubule nucleation pathways and their mode of action still need to be identified. Using purified Xenopus laevis proteins we biochemically reconstitute branching microtubule nucleation, ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49797
更新日期:2020-01-14 00:00:00
abstract::Synonymous codon usage (SCU) varies widely among human genes. In particular, genes involved in different functional categories display a distinct codon usage, which was interpreted as evidence that SCU is adaptively constrained to optimize translation efficiency in distinct cellular states. We demonstrate here that SC...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.27344
更新日期:2017-08-15 00:00:00
abstract::Mitochondrial dysfunction is associated with activation of the integrated stress response (ISR) but the underlying triggers remain unclear. We systematically combined acute mitochondrial inhibitors with genetic tools for compartment-specific NADH oxidation to trace mechanisms linking different forms of mitochondrial d...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49178
更新日期:2020-05-28 00:00:00
abstract::Anterograde transport of late endosomes or lysosomes (LE/Lys) is crucial for proper axon growth. However, the role of energetic nutrients has been poorly explored. Malonyl-CoA is a precursor of fatty acids, and its intracellular levels highly fluctuate depending on glucose availability or the energy sensor AMP-activat...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51063
更新日期:2019-12-23 00:00:00