当前位置： SCI文献检索 > Nature Communications期刊下所有文献 > Endothelial cell rearrangements during vascular patterning require PI3-kinase-mediated inhibition of actomyosin contractility.

Endothelial cell rearrangements during vascular patterning require PI3-kinase-mediated inhibition of actomyosin contractility.

Abstract：

:Angiogenesis is a dynamic process relying on endothelial cell rearrangements within vascular tubes, yet the underlying mechanisms and functional relevance are poorly understood. Here we show that PI3Kα regulates endothelial cell rearrangements using a combination of a PI3Kα-selective inhibitor and endothelial-specific genetic deletion to abrogate PI3Kα activity during vessel development. Quantitative phosphoproteomics together with detailed cell biology analyses in vivo and in vitro reveal that PI3K signalling prevents NUAK1-dependent phosphorylation of the myosin phosphatase targeting-1 (MYPT1) protein, thereby allowing myosin light chain phosphatase (MLCP) activity and ultimately downregulating actomyosin contractility. Decreased PI3K activity enhances actomyosin contractility and impairs junctional remodelling and stabilization. This leads to overstretched endothelial cells that fail to anastomose properly and form aberrant superimposed layers within the vasculature. Our findings define the PI3K/NUAK1/MYPT1/MLCP axis as a critical pathway to regulate actomyosin contractility in endothelial cells, supporting vascular patterning and expansion through the control of cell rearrangement.

journal_name

Nat Commun

journal_title

Nature communications

authors

Angulo-Urarte A,Casado P,Castillo SD,Kobialka P,Kotini MP,Figueiredo AM,Castel P,Rajeeve V,Milà-Guasch M,Millan J,Wiesner C,Serra H,Muixi L,Casanovas O,Viñals F,Affolter M,Gerhardt H,Huveneers S,Belting HG,Cutillas

doi

10.1038/s41467-018-07172-3

subject

Has Abstract

pub_date

2018-11-16 00:00:00

pages

4826

issue

1

issn

2041-1723

pii

10.1038/s41467-018-07172-3

journal_volume

9

pub_type

杂志文章

相关文献

Nature Communications文献大全
  • Oxidising agents in sub-arc mantle melts link slab devolatilisation and arc magmas.

    abstract：:Subduction zone magmas are more oxidised on eruption than those at mid-ocean ridges. This is attributed either to oxidising components, derived from subducted lithosphere (slab) and added to the mantle wedge, or to oxidation processes occurring during magma ascent via differentiation. Here we provide direct evidence f...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-05804-2

    authors： Bénard A,Klimm K,Woodland AB,Arculus RJ,Wilke M,Botcharnikov RE,Shimizu N,Nebel O,Rivard C,Ionov DA

    更新日期：2018-08-29 00:00:00

  • Formation pathways of mesoporous silica nanoparticles with dodecagonal tiling.

    abstract：:Considerable progress in the fabrication of quasicrystals demonstrates that they can be realized in a broad range of materials. However, the development of chemistries enabling direct experimental observation of early quasicrystal growth pathways remains challenging. Here, we report the synthesis of four surfactant-di...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-017-00351-8

    authors： Sun Y,Ma K,Kao T,Spoth KA,Sai H,Zhang D,Kourkoutis LF,Elser V,Wiesner U

    更新日期：2017-08-15 00:00:00

  • RNA structure determination by solid-state NMR spectroscopy.

    abstract：:Knowledge of the RNA three-dimensional structure, either in isolation or as part of RNP complexes, is fundamental to understand the mechanism of numerous cellular processes. Because of its flexibility, RNA represents a challenge for crystallization, while the large size of cellular complexes brings solution-state NMR ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms8024

    authors： Marchanka A,Simon B,Althoff-Ospelt G,Carlomagno T

    更新日期：2015-05-11 00:00:00

  • Epigenetic engineering reveals a balance between histone modifications and transcription in kinetochore maintenance.

    abstract：:Centromeres consist of specialized centrochromatin containing CENP-A nucleosomes intermingled with H3 nucleosomes carrying transcription-associated modifications. We have designed a novel synthetic biology 'in situ epistasis' analysis in which H3 dimethylated on lysine 4 (H3K4me2) demethylase LSD2 plus synthetic modul...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms13334

    authors： Molina O,Vargiu G,Abad MA,Zhiteneva A,Jeyaprakash AA,Masumoto H,Kouprina N,Larionov V,Earnshaw WC

    更新日期：2016-11-14 00:00:00

  • African-centric TP53 variant increases iron accumulation and bacterial pathogenesis but improves response to malaria toxin.

    abstract：:A variant at amino acid 47 in human TP53 exists predominantly in individuals of African descent. P47S human and mouse cells show increased cancer risk due to defective ferroptosis. Here, we show that this ferroptotic defect causes iron accumulation in P47S macrophages. This high iron content alters macrophage cytokine...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-14151-9

    authors： Singh KS,Leu JI,Barnoud T,Vonteddu P,Gnanapradeepan K,Lin C,Liu Q,Barton JC,Kossenkov AV,George DL,Murphy ME,Dotiwala F

    更新日期：2020-01-24 00:00:00

  • Nano-photosensitizer based on layered double hydroxide and isophthalic acid for singlet oxygenation and photodynamic therapy.

    abstract：:Singlet oxygen has won a great deal of attention to catalysis and biological studies due to its strong oxidizing properties. However, the photosensitizers which require for the generation of singlet oxygen remain inadequate because of their lack of long-wavelength absorption, weak hydrophilicity, and poor biocompatibi...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-05223-3

    authors： Gao R,Mei X,Yan D,Liang R,Wei M

    更新日期：2018-07-18 00:00:00

  • Distinct transcriptional roles for Histone H3-K56 acetylation during the cell cycle in Yeast.

    abstract：:Dynamic disruption and reassembly of promoter-proximal nucleosomes is a conserved hallmark of transcriptionally active chromatin. Histone H3-K56 acetylation (H3K56Ac) enhances these turnover events and promotes nucleosome assembly during S phase. Here we sequence nascent transcripts to investigate the impact of H3K56A...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-12400-5

    authors： Topal S,Vasseur P,Radman-Livaja M,Peterson CL

    更新日期：2019-09-26 00:00:00

  • Integrating geological archives and climate models for the mid-Pliocene warm period.

    abstract：:The mid-Pliocene Warm Period (mPWP) offers an opportunity to understand a warmer-than-present world and assess the predictive ability of numerical climate models. Environmental reconstruction and climate modelling are crucial for understanding the mPWP, and the synergy of these two, often disparate, fields has proven ...

    journal_title：Nature communications

    pub_type： 杂志文章,评审

    doi：10.1038/ncomms10646

    authors： Haywood AM,Dowsett HJ,Dolan AM

    更新日期：2016-02-16 00:00:00

  • Ultra-low carrier concentration and surface-dominant transport in antimony-doped Bi₂Se₃ topological insulator nanoribbons.

    abstract：:A topological insulator is the state of quantum matter possessing gapless spin-locking surface states across the bulk band gap, which has created new opportunities from novel electronics to energy conversion. However, the large concentration of bulk residual carriers has been a major challenge for revealing the proper...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms1771

    authors： Hong SS,Cha JJ,Kong D,Cui Y

    更新日期：2012-03-27 00:00:00

  • Enhanced acoustic sensing through wave compression and pressure amplification in anisotropic metamaterials.

    abstract：:Acoustic sensors play an important role in many areas, such as homeland security, navigation, communication, health care and industry. However, the fundamental pressure detection limit hinders the performance of current acoustic sensing technologies. Here, through analytical, numerical and experimental studies, we sho...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms6247

    authors： Chen Y,Liu H,Reilly M,Bae H,Yu M

    更新日期：2014-10-15 00:00:00

  • NOTCH1 activation compensates BRCA1 deficiency and promotes triple-negative breast cancer formation.

    abstract：:BRCA1 mutation carriers have a higher risk of developing triple-negative breast cancer (TNBC), which is a refractory disease due to its non-responsiveness to current clinical targeted therapies. Using the Sleeping Beauty transposon system in Brca1-deficient mice, we identified 169 putative cancer drivers, among which ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-16936-9

    authors： Miao K,Lei JH,Valecha MV,Zhang A,Xu J,Wang L,Lyu X,Chen S,Miao Z,Zhang X,Su SM,Shao F,Rajendran BK,Bao J,Zeng J,Sun H,Chen P,Tan K,Chen Q,Wong KH,Xu X,Deng CX

    更新日期：2020-06-26 00:00:00

  • Rapid laccolith intrusion driven by explosive volcanic eruption.

    abstract：:Magmatic intrusions and volcanic eruptions are intimately related phenomena. Shallow magma intrusion builds subsurface reservoirs that are drained by volcanic eruptions. Thus, the long-held view is that intrusions must precede and feed eruptions. Here we show that explosive eruptions can also cause magma intrusion. We...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms13585

    authors： Castro JM,Cordonnier B,Schipper CI,Tuffen H,Baumann TS,Feisel Y

    更新日期：2016-11-23 00:00:00

  • The anti-sigma factor RsrA responds to oxidative stress by reburying its hydrophobic core.

    abstract：:Redox-regulated effector systems that counteract oxidative stress are essential for all forms of life. Here we uncover a new paradigm for sensing oxidative stress centred on the hydrophobic core of a sensor protein. RsrA is an archetypal zinc-binding anti-sigma factor that responds to disulfide stress in the cytoplasm...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms12194

    authors： Rajasekar KV,Zdanowski K,Yan J,Hopper JT,Francis ML,Seepersad C,Sharp C,Pecqueur L,Werner JM,Robinson CV,Mohammed S,Potts JR,Kleanthous C

    更新日期：2016-07-19 00:00:00

  • IRSp53 controls plasma membrane shape and polarized transport at the nascent lumen in epithelial tubules.

    abstract：:It is unclear whether the establishment of apical-basal cell polarity during the generation of epithelial lumens requires molecules acting at the plasma membrane/actin interface. Here, we show that the I-BAR-containing IRSp53 protein controls lumen formation and the positioning of the polarity determinants aPKC and po...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-17091-x

    authors： Bisi S,Marchesi S,Rizvi A,Carra D,Beznoussenko GV,Ferrara I,Deflorian G,Mironov A,Bertalot G,Pisati F,Oldani A,Cattaneo A,Saberamoli G,Pece S,Viale G,Bachi A,Tripodo C,Scita G,Disanza A

    更新日期：2020-07-14 00:00:00

  • GWAS for Interleukin-1β levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation.

    abstract：:There is no agnostic GWAS evidence for the genetic control of IL-1β expression in periodontal disease. Here we report a GWAS for "high" gingival crevicular fluid IL-1β expression among 4910 European-American adults and identify association signals in the IL37 locus. rs3811046 at this locus (p = 3.3 × 10-22) is associa...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-05940-9

    authors： Offenbacher S,Jiao Y,Kim SJ,Marchesan J,Moss KL,Jing L,Divaris K,Bencharit S,Agler CS,Morelli T,Zhang S,Sun L,Seaman WT,Cowley D,Barros SP,Beck JD,Munz M,Schaefer AS,North KE

    更新日期：2018-09-11 00:00:00

  • Diversity-oriented synthetic strategy for developing a chemical modulator of protein-protein interaction.

    abstract：:Diversity-oriented synthesis (DOS) can provide a collection of diverse and complex drug-like small molecules, which is critical in the development of new chemical probes for biological research of undruggable targets. However, the design and synthesis of small-molecule libraries with improved biological relevance as w...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms13196

    authors： Kim J,Jung J,Koo J,Cho W,Lee WS,Kim C,Park W,Park SB

    更新日期：2016-10-24 00:00:00

  • Two-dimensional molecular brush-functionalized porous bilayer composite separators toward ultrastable high-current density lithium metal anodes.

    abstract：:Lithium metal batteries have been considerably limited by the problems of uncontrolled dendritic lithium formation and the highly reactive nature of lithium with electrolytes. Herein, we have developed functional porous bilayer composite separators by simply blade-coating polyacrylamide-grafted graphene oxide molecula...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-09211-z

    authors： Li C,Liu S,Shi C,Liang G,Lu Z,Fu R,Wu D

    更新日期：2019-03-25 00:00:00

  • Targeting cell surface TLR7 for therapeutic intervention in autoimmune diseases.

    abstract：:Toll-like receptor 7 (TLR7) senses microbial-derived RNA but can also potentially respond to self-derived RNA. To prevent autoimmune responses, TLR7 is thought to localize in endolysosomes. Contrary to this view, we show here that TLR7 is present on the cell surface of immune cells and that TLR7 responses can be inhib...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms7119

    authors： Kanno A,Tanimura N,Ishizaki M,Ohko K,Motoi Y,Onji M,Fukui R,Shimozato T,Yamamoto K,Shibata T,Sano S,Sugahara-Tobinai A,Takai T,Ohto U,Shimizu T,Saitoh S,Miyake K

    更新日期：2015-02-04 00:00:00

  • NK1.1+ CD8+ T cells escape TGF-β control and contribute to early microbial pathogen response.

    abstract：:Following microbial pathogen invasion, one of the main challenges for the host is to rapidly control pathogen spreading to avoid vital tissue damage. Here we report that an effector CD8(+) T-cell population that expresses the marker NK1.1 undergoes delayed contraction and sustains early anti-microbial protection. NK1....

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms6150

    authors： Ruiz AL,Soudja SM,Deceneux C,Lauvau G,Marie JC

    更新日期：2014-10-06 00:00:00

  • Author Correction: Identification of multiple risk loci and regulatory mechanisms influencing susceptibility to multiple myeloma.

    abstract：:The original version of this Article contained an error in the spelling of a member of the PRACTICAL Consortium, Manuela Gago-Dominguez, which was incorrectly given as Manuela Gago Dominguez. This has now been corrected in both the PDF and HTML versions of the Article. Furthermore, in the original HTML version of this...

    journal_title：Nature communications

    pub_type： 杂志文章,已发布勘误

    doi：10.1038/s41467-018-08107-8

    authors： Went M,Sud A,Försti A,Halvarsson BM,Weinhold N,Kimber S,van Duin M,Thorleifsson G,Holroyd A,Johnson DC,Li N,Orlando G,Law PJ,Ali M,Chen B,Mitchell JS,Gudbjartsson DF,Kuiper R,Stephens OW,Bertsch U,Broderick P,Ca

    更新日期：2019-01-10 00:00:00

  • Transneuronal delivery of hyper-interleukin-6 enables functional recovery after severe spinal cord injury in mice.

    abstract：:Spinal cord injury (SCI) often causes severe and permanent disabilities due to the regenerative failure of severed axons. Here we report significant locomotor recovery of both hindlimbs after a complete spinal cord crush. This is achieved by the unilateral transduction of cortical motoneurons with an AAV expressing hy...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-20112-4

    authors： Leibinger M,Zeitler C,Gobrecht P,Andreadaki A,Gisselmann G,Fischer D

    更新日期：2021-01-15 00:00:00

  • circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity.

    abstract：:Circular RNAs (circRNA) are a class of covalently closed single-stranded RNAs that have been implicated in cancer progression. Here we identify circNDUFB2 to be downregulated in non-small cell lung cancer (NSCLC) tissues, and to negatively correlate with NSCLC malignant features. Elevated circNDUFB2 inhibits growth an...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-20527-z

    authors： Li B,Zhu L,Lu C,Wang C,Wang H,Jin H,Ma X,Cheng Z,Yu C,Wang S,Zuo Q,Zhou Y,Wang J,Yang C,Lv Y,Jiang L,Qin W

    更新日期：2021-01-12 00:00:00

  • Hepatic Ago2-mediated RNA silencing controls energy metabolism linked to AMPK activation and obesity-associated pathophysiology.

    abstract：:RNA silencing inhibits mRNA translation. While mRNA translation accounts for the majority of cellular energy expenditure, it is unclear if RNA silencing regulates energy homeostasis. Here, we report that hepatic Argonaute 2 (Ago2)-mediated RNA silencing regulates both intrinsic energy production and consumption and di...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-05870-6

    authors： Zhang C,Seo J,Murakami K,Salem ESB,Bernhard E,Borra VJ,Choi K,Yuan CL,Chan CC,Chen X,Huang T,Weirauch MT,Divanovic S,Qi NR,Thomas HE,Mercer CA,Siomi H,Nakamura T

    更新日期：2018-09-10 00:00:00

  • Expansion of a superconducting vortex core into a diffusive metal.

    abstract：:Vortices in quantum condensates exist owing to a macroscopic phase coherence. Here we show, both experimentally and theoretically, that a quantum vortex with a well-defined core can exist in a rather thick normal metal, proximized with a superconductor. Using scanning tunneling spectroscopy we reveal a proximity vorte...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-04582-1

    authors： Stolyarov VS,Cren T,Brun C,Golovchanskiy IA,Skryabina OV,Kasatonov DI,Khapaev MM,Kupriyanov MY,Golubov AA,Roditchev D

    更新日期：2018-06-11 00:00:00

  • Diffusion in translucent media.

    abstract：:Diffusion is the result of repeated random scattering. It governs a wide range of phenomena from Brownian motion, to heat flow through window panes, neutron flux in fuel rods, dispersion of light in human tissue, and electronic conduction. It is universally acknowledged that the diffusion approach to describing wave t...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-04242-4

    authors： Shi Z,Genack AZ

    更新日期：2018-05-10 00:00:00

  • A comprehensive examination of Nanopore native RNA sequencing for characterization of complex transcriptomes.

    abstract：:A platform for highly parallel direct sequencing of native RNA strands was recently described by Oxford Nanopore Technologies, but despite initial efforts it remains crucial to further investigate the technology for quantification of complex transcriptomes. Here we undertake native RNA sequencing of polyA + RNA from t...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-11272-z

    authors： Soneson C,Yao Y,Bratus-Neuenschwander A,Patrignani A,Robinson MD,Hussain S

    更新日期：2019-07-31 00:00:00

  • Ethanol consumption inhibits TFH cell responses and the development of autoimmune arthritis.

    abstract：:Alcohol consumption is a consistent protective factor for the development of autoimmune diseases such as rheumatoid arthritis (RA). The underlying mechanism for this tolerance-inducing effect of alcohol, however, is unknown. Here we show that alcohol and its metabolite acetate alter the functional state of T follicula...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-15855-z

    authors： Azizov V,Dietel K,Steffen F,Dürholz K,Meidenbauer J,Lucas S,Frech M,Omata Y,Tajik N,Knipfer L,Kolenbrander A,Seubert S,Lapuente D,Sokolova MV,Hofmann J,Tenbusch M,Ramming A,Steffen U,Nimmerjahn F,Linker R,Wirtz S

    更新日期：2020-04-24 00:00:00

  • Publisher Correction: Role of outer surface probes for regulating ion gating of nanochannels.

    abstract：:The original version of this Article contained an error in Fig. 3. The scale bars in Figs 3c and 3d were incorrectly labelled as 50 μA. In the correct version, the scale bars are labelled as 0.5 μA. This has now been corrected in both the PDF and HTML versions of the Article. ...

    journal_title：Nature communications

    pub_type： 杂志文章,已发布勘误

    doi：10.1038/s41467-018-03030-4

    authors： Li X,Zhai T,Gao P,Cheng H,Hou R,Lou X,Xia F

    更新日期：2018-02-08 00:00:00

  • MLKL trafficking and accumulation at the plasma membrane control the kinetics and threshold for necroptosis.

    abstract：:Mixed lineage kinase domain-like (MLKL) is the terminal protein in the pro-inflammatory necroptotic cell death program. RIPK3-mediated phosphorylation is thought to initiate MLKL oligomerization, membrane translocation and membrane disruption, although the precise choreography of events is incompletely understood. Her...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-16887-1

    authors： Samson AL,Zhang Y,Geoghegan ND,Gavin XJ,Davies KA,Mlodzianoski MJ,Whitehead LW,Frank D,Garnish SE,Fitzgibbon C,Hempel A,Young SN,Jacobsen AV,Cawthorne W,Petrie EJ,Faux MC,Shield-Artin K,Lalaoui N,Hildebrand JM,Silke

    更新日期：2020-06-19 00:00:00

  • Kin discrimination allows plants to modify investment towards pollinator attraction.

    abstract：:Pollinators tend to be preferentially attracted to large floral displays that may comprise more than one plant in a patch. Attracting pollinators thus not only benefits individuals investing in advertising, but also other plants in a patch through a 'magnet' effect. Accordingly, there could be an indirect fitness adva...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-04378-3

    authors： Torices R,Gómez JM,Pannell JR

    更新日期：2018-05-22 00:00:00