RNA structure determination by solid-state NMR spectroscopy.

Abstract:

:Knowledge of the RNA three-dimensional structure, either in isolation or as part of RNP complexes, is fundamental to understand the mechanism of numerous cellular processes. Because of its flexibility, RNA represents a challenge for crystallization, while the large size of cellular complexes brings solution-state NMR to its limits. Here, we demonstrate an alternative approach on the basis of solid-state NMR spectroscopy. We develop a suite of experiments and RNA labeling schemes and demonstrate for the first time that ssNMR can yield a RNA structure at high-resolution. This methodology allows structural analysis of segmentally labelled RNA stretches in high-molecular weight cellular machines—independent of their ability to crystallize—and opens the way to mechanistic studies of currently difficult-to-access RNA-protein assemblies.

journal_name

Nat Commun

journal_title

Nature communications

authors

Marchanka A,Simon B,Althoff-Ospelt G,Carlomagno T

doi

10.1038/ncomms8024

subject

Has Abstract

pub_date

2015-05-11 00:00:00

pages

7024

issn

2041-1723

pii

ncomms8024

journal_volume

6

pub_type

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