当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES期刊下所有文献 > Toxicity Evaluation and Biomarker Selection with Validated Reference Gene in Embryonic Zebrafish Exposed to Mitoxantrone.

Toxicity Evaluation and Biomarker Selection with Validated Reference Gene in Embryonic Zebrafish Exposed to Mitoxantrone.

Abstract：

:Notwithstanding the widespread use and promising clinical value of chemotherapy, the pharmacokinetics, toxicology, and mechanism of mitoxantrone remains unclear. To promote the clinical value in the treatment of human diseases and the exploration of potential subtle effects of mitoxantrone, zebrafish embryos were employed to evaluate toxicity with validated reference genes based on independent stability evaluation programs. The most stable and recommended reference gene was gapdh, followed by tubα1b, for the 48 h post fertilization (hpf) zebrafish embryo mitoxantrone test, while both eef1a1l1 and rpl13α were recommended as reference genes for the 96 hpf zebrafish embryo mitoxantrone test. With gapdh as an internal control, we analyzed the mRNA levels of representative hepatotoxicity biomarkers, including fabp10a, gclc, gsr, nqo1, cardiotoxicity biomarker erg, and neurotoxicity biomarker gfap in the 48 hpf embryo mitoxantrone test. The mRNA levels of gclc, gsr, and gfap increased significantly in 10 and 50 μg/L mitoxantrone-treated 48 hpf embryos, while the transcript levels of fabp10a decreased in a dose-dependent manner, indicating that mitoxantrone induced hepatotoxicity and neurotoxicity. Liver hematoxylin⁻eosin staining and the spontaneous movement of embryos confirmed the results. Thus, the present research suggests that mitoxantrone induces toxicity during the development of the liver and nervous system in zebrafish embryos and that fabp10a is recommended as a potential biomarker for hepatotoxicity in zebrafish embryos. Additionally, gapdh is proposed as a reference gene for the 48 hpf zebrafish embryo mitoxantrone toxicity test, while eef1a1l1 and rpl13α are proposed as that for the 96 hpf test.

journal_name

Int J Mol Sci

journal_title

International journal of molecular sciences

authors

Liu L,Zhu H,Yan Y,Lv P,Wu W

doi

10.3390/ijms19113516

subject

Has Abstract

pub_date

2018-11-08 00:00:00

issue

11

issn

1422-0067

pii

ijms19113516

journal_volume

19

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES文献大全
  • CES2, ABCG2, TS and Topo-I primary and synchronous metastasis expression and clinical outcome in metastatic colorectal cancer patients treated with first-line FOLFIRI regimen.

    abstract：:Enzymatic activation of irinotecan (CPT-11) is due to carboxylesterase (CES), and its pharmacological behavior is influenced by drug resistance-related proteins. We previously reported that the clinical response and prognosis of metastatic colorectal cancer (mCRC) patients did not differ in tumors with different thymi...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms150915767

    authors： Silvestris N,Simone G,Partipilo G,Scarpi E,Lorusso V,Brunetti AE,Maiello E,Paradiso A,Mangia A

    更新日期：2014-09-05 00:00:00

  • Bioinformatic Reconstruction and Analysis of Gene Networks Related to Glucose Variability in Diabetes and Its Complications.

    abstract：:Glucose variability (GV) has been recognized recently as a promoter of complications and therapeutic targets in diabetes. The aim of this study was to reconstruct and analyze gene networks related to GV in diabetes and its complications. For network analysis, we used the ANDSystem that provides automatic network recon...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21228691

    authors： Saik OV,Klimontov VV

    更新日期：2020-11-18 00:00:00

  • Correction: Xingchen Zhou, et al. Whole Exome Sequencing in Psoriasis Patients Contributes to Studies of Acitretin Treatment Difference. Int. J. Mol. Sci. 2017, 18, 295.

    abstract：:We would like to submit the following correction to the published paper [1], the reason for this action is that the data in Table 3 were reanalyzed by one more accurate statistic method: On page 12, the sentence of paragraph three "OR and 95% CI were calculated by limited backward-LR (likelihood ratio) logistic regres...

    journal_title：International journal of molecular sciences

    pub_type： 已发布勘误

    doi：10.3390/ijms18091899

    authors： Zhou X,He Y,Kuang Y,Li J,Zhang J,Chen M,Chen W,Su J,Zhao S,Liu P,Chen M,Shen M,Chen X,Zhu W,Chen X

    更新日期：2017-09-04 00:00:00

  • FKBP5 Gene Expression Predicts Antidepressant Treatment Outcome in Depression.

    abstract：:Adverse experiences and chronic stress are well-known risk factors for the development of major depression, and an impaired stress response regulation is frequently observed in acute depression. Impaired glucocorticoid receptor (GR) signalling plays an important role in these alterations, and a restoration of GR signa...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20030485

    authors： Ising M,Maccarrone G,Brückl T,Scheuer S,Hennings J,Holsboer F,Turck CW,Uhr M,Lucae S

    更新日期：2019-01-23 00:00:00

  • Elucidation of the specific formation of homo- and heterodimeric forms of ThbZIP1 and its role in stress.

    abstract：:Protein-protein interactions are important for the molecular understanding of the biological processes of proteins. The dimerization of bZIPs (basic leucine zipper proteins) is involved in modifying binding site specificities, altering dimer stability, and permitting a new set of specific protein-to-protein interactio...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms150610005

    authors： Nie X,Ji X,Liu Y,Zheng L,Wang Y

    更新日期：2014-06-04 00:00:00

  • Fruit Antioxidants during Vinegar Processing: Changes in Content and in Vitro Bio-Accessibility.

    abstract：BACKGROUND:Vinegars based on fruit juices could conserve part of the health-associated compounds present in the fruits. However, in general very limited knowledge exists on the consequences of vinegar-making on different antioxidant compounds from fruit. In this study vinegars derived from apple and grape are studied. ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms17101658

    authors： Bakir S,Toydemir G,Boyacioglu D,Beekwilder J,Capanoglu E

    更新日期：2016-09-29 00:00:00

  • Combinatorial Drug Treatments Reveal Promising Anticytomegaloviral Profiles for Clinically Relevant Pharmaceutical Kinase Inhibitors (PKIs).

    abstract：:Human cytomegalovirus (HCMV) is a human pathogenic herpesvirus associated with a variety of clinical symptoms. Current antiviral therapy is not always effective, so that improved drug classes and drug-targeting strategies are needed. Particularly host-directed antivirals, including pharmaceutical kinase inhibitors (PK...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms22020575

    authors： Wild M,Kicuntod J,Seyler L,Wangen C,Bertzbach LD,Conradie AM,Kaufer BB,Wagner S,Michel D,Eickhoff J,Tsogoeva SB,Bäuerle T,Hahn F,Marschall M

    更新日期：2021-01-08 00:00:00

  • Impact of Fetuin-A (AHSG) on Tumor Progression and Type 2 Diabetes.

    abstract：:Fetuin-A is the protein product of the AHSG gene in humans. It is mainly synthesized by the liver in adult humans and is secreted into the blood where its concentration can vary from a low of ~0.2 mg/mL to a high of ~0.8 mg/mL. Presently, it is considered to be a multifunctional protein that plays important roles in d...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms19082211

    authors： Ochieng J,Nangami G,Sakwe A,Moye C,Alvarez J,Whalen D,Thomas P,Lammers P

    更新日期：2018-07-29 00:00:00

  • Rack1 Mediates the Interaction of P-Glycoprotein with Anxa2 and Regulates Migration and Invasion of Multidrug-Resistant Breast Cancer Cells.

    abstract：:The emergence of multidrug resistance is always associated with more rapid tumor recurrence and metastasis. P-glycoprotein (P-gp), which is a well-known multidrug-efflux transporter, confers enhanced invasion ability in drug-resistant cells. Previous studies have shown that P-gp probably exerts its tumor-promoting fun...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms17101718

    authors： Yang Y,Wu N,Wang Z,Zhang F,Tian R,Ji W,Ren X,Niu R

    更新日期：2016-10-13 00:00:00

  • A Rationale for Hypoxic and Chemical Conditioning in Huntington's Disease.

    abstract：:Neurodegenerative diseases are characterized by adverse cellular environments and pathological alterations causing neurodegeneration in distinct brain regions. This development is triggered or facilitated by conditions such as hypoxia, ischemia or inflammation and is associated with disruptions of fundamental cellular...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms22020582

    authors： Burtscher J,Maglione V,Di Pardo A,Millet GP,Schwarzer C,Zangrandi L

    更新日期：2021-01-08 00:00:00

  • N-Caffeoyltryptamine, a Potent Anti-Inflammatory Phenolic Amide, Suppressed MCP-1 Expression in LPS-stimulated THP-1 Cells and Rats Fed a High-Fat Diet.

    abstract：:Monocyte chemoattractant protein-1 (MCP-1) is a well-known chemokine critically involved in the pathophysiological progression of several inflammatory diseases including arthrosclerosis. N-caffeoyltryptamine is a phenolic amide with strong anti-inflammatory effects. Therefore, in this paper, the potential effect of N-...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms18061148

    authors： Park JB

    更新日期：2017-05-27 00:00:00

  • Role of Tricellular Tight Junction Protein Lipolysis-Stimulated Lipoprotein Receptor (LSR) in Cancer Cells.

    abstract：:Maintaining a robust epithelial barrier requires the accumulation of tight junction proteins, LSR/angulin-1 and tricellulin, at the tricellular contacts. Alterations in the localization of these proteins temporarily cause epithelial barrier dysfunction, which is closely associated with not only physiological different...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms20143555

    authors： Kohno T,Konno T,Kojima T

    更新日期：2019-07-20 00:00:00

  • Celebrating 25 Years of MicroRNA Research: From Discovery to Clinical Application.

    abstract：:In 1993, the Ambros lab reported the cloning and developmental function of lin-4, the first microRNA [...]. ...

    journal_title：International journal of molecular sciences

    pub_type： 社论

    doi：10.3390/ijms20081987

    authors： Sempere LF

    更新日期：2019-04-23 00:00:00

  • Chemical constituents analysis and antidiabetic activity validation of four fern species from Taiwan.

    abstract：:Pterosins are abundant in ferns, and pterosin A was considered a novel activator of adenosine monophosphate-activated protein kinase, which is crucial for regulating blood glucose homeostasis. However, the distribution of pterosins in different species of ferns from various places in Taiwan is currently unclear. To ad...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms16022497

    authors： Chen CY,Chiu FY,Lin Y,Huang WJ,Hsieh PS,Hsu FL

    更新日期：2015-01-22 00:00:00

  • Cathecol and Naphtol Groups in Salphen-Type Schiff Bases for the Preparation of Polynuclear Complexes.

    abstract：:In this paper, we show a strategy to modify salphen-type Schiff base ligands with naphtol (SYML1) and pyrocathecol (2,3-dihydroxyphenyl) groups (SYML2), or a combination of both (ASYML). Each of these ligands can be used to obtain polynuclear metal complexes following two different strategies. One relies on using meta...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21103574

    authors： Gholizadeh Dogaheh S,Barbero S,Barrientos J,Janczak J,Soleimannejad J,Sañudo EC

    更新日期：2020-05-18 00:00:00

  • Vitamin D Supplementation Reduces Both Oxidative DNA Damage and Insulin Resistance in the Elderly with Metabolic Disorders.

    abstract：BACKGROUND:Research evidence indicates that vitamin D deficiency is involved in the pathogenesis of insulin resistance (IR) and associated metabolic disorders including hyperglycemia and dyslipidemia. It also suggested that vitamin D deficiency is associated with elevated levels of oxidative stress and its complication...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,随机对照试验

    doi：10.3390/ijms20122891

    authors： Wenclewska S,Szymczak-Pajor I,Drzewoski J,Bunk M,Śliwińska A

    更新日期：2019-06-13 00:00:00

  • Develop a High-Throughput Screening Method to Identify C-P4H1 (Collagen Prolyl 4-Hydroxylase 1) Inhibitors from FDA-Approved Chemicals.

    abstract：:Collagen prolyl 4-hydroxylase 1 (C-P4H1) is an α-ketoglutarate (α-KG)-dependent dioxygenase that catalyzes 4-hydroxylation of proline on collagen. C-P4H1-induced prolyl hydroxylation is required for proper collagen deposition and cancer metastasis. Therefore, targeting C-P4H1 is considered a potential therapeutic stra...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21186613

    authors： Wang S,Lee KH,Araujo NV,Zhan CG,Rangnekar VM,Xu R

    更新日期：2020-09-10 00:00:00

  • Probing antigen-antibody interaction using fluorescence coupled capillary electrophoresis.

    abstract：:In this report, the use of fluorescence detection coupled capillary electrophoresis (CE-FL) allowed us to fully characterize the antigen-antibody interaction. CE-FL allowed separation of unbound quantum dots (QDs) and ligand bound QDs and also revealed an ordered assembly of biomolecules on QDs. Further, we observed F...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms140919146

    authors： Wang J,Qiu L,Wang C,Zhang Y,Li J,Xia J,Jiang P

    更新日期：2013-09-17 00:00:00

  • Decreased Expression of Vitamin D Receptor Affects an Immune Response in Primary Biliary Cholangitis via the VDR-miRNA155-SOCS1 Pathway.

    abstract：:Primary biliary cholangitis (PBC) is an immune-mediated cholestatic disease. Vitamin D receptor (VDR)-dependent signaling constrains an inflammatory response by targeting the miRNA155-SOCS1 (suppressor of cytokine signaling 1) axis. The VDR-miRNA155-SOCS1 pathway was investigated in the context of the autoimmune respo...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms18020289

    authors： Kempinska-Podhorodecka A,Milkiewicz M,Wasik U,Ligocka J,Zawadzki M,Krawczyk M,Milkiewicz P

    更新日期：2017-01-29 00:00:00

  • Reply to the Letter to the Editor by Li et al.: Bioinformatics Analysis in Mice with Diet-Induced Nonalcoholic Steatohepatitis Treated with Astaxanthin and Vitamin E.

    abstract：:n/a. ...

    journal_title：International journal of molecular sciences

    pub_type： 评论,杂志文章

    doi：10.3390/ijms18050994

    authors： Kobori M,Takahashi Y,Sakurai M,Ni Y,Chen G,Nagashimada M,Kaneko S,Ota T

    更新日期：2017-05-05 00:00:00

  • Vitamin D Supplementation in Central Nervous System Demyelinating Disease-Enough Is Enough.

    abstract：:The exact cause of multiple sclerosis (MS) remains elusive. Various factors, however, have been identified that increase an individual's risk of developing this central nervous system (CNS) demyelinating disease and are associated with an acceleration in disease severity. Besides genetic determinants, environmental fa...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms20010218

    authors： Häusler D,Weber MS

    更新日期：2019-01-08 00:00:00

  • The Role of Cysteine Cathepsins in Cancer Progression and Drug Resistance.

    abstract：:Cysteine cathepsins are lysosomal enzymes belonging to the papain family. Their expression is misregulated in a wide variety of tumors, and ample data prove their involvement in cancer progression, angiogenesis, metastasis, and in the occurrence of drug resistance. However, while their overexpression is usually associ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms20143602

    authors： Rudzińska M,Parodi A,Soond SM,Vinarov AZ,Korolev DO,Morozov AO,Daglioglu C,Tutar Y,Zamyatnin AA Jr

    更新日期：2019-07-23 00:00:00

  • Intergenerational Metabolomic Analysis of Mothers with a History of Gestational Diabetes Mellitus and Their Offspring.

    abstract：:Shared metabolomic patterns at delivery have been suggested to underlie the mother-to-child transmission of adverse metabolic health. This study aimed to investigate whether mothers with gestational diabetes mellitus (GDM) and their offspring show similar metabolomic patterns several years postpartum. Targeted metabol...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21249647

    authors： Ott R,Pawlow X,Weiß A,Hofelich A,Herbst M,Hummel N,Prehn C,Adamski J,Römisch-Margl W,Kastenmüller G,Ziegler AG,Hummel S

    更新日期：2020-12-17 00:00:00

  • Current Insights into Oral Cancer Epigenetics.

    abstract：:Epigenetic modifications have emerged into one of the cancer hallmarks, replacing the concept of malignant pathologies as being solely genetic-based conditions. The epigenetic landscape is responsible for normal development but also for the heterogeneity among tissues in terms of gene expression patterns. Dysregulatio...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms19030670

    authors： Irimie AI,Ciocan C,Gulei D,Mehterov N,Atanasov AG,Dudea D,Berindan-Neagoe I

    更新日期：2018-02-27 00:00:00

  • Molecular Signature of Indeterminate Thyroid Lesions: Current Methods to Improve Fine Needle Aspiration Cytology (FNAC) Diagnosis.

    abstract：:Fine needle aspiration cytology (FNAC) represents the gold standard for determining the nature of thyroid nodules. It is a reliable method with good sensitivity and specificity. However, indeterminate lesions remain a diagnostic challenge and researchers have contributed molecular markers to search for in cytological ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms18040775

    authors： Cantara S,Marzocchi C,Pilli T,Cardinale S,Forleo R,Castagna MG,Pacini F

    更新日期：2017-04-06 00:00:00

  • Filamentous Aggregates of Tau Proteins Fulfil Standard Amyloid Criteria Provided by the Fuzzy Oil Drop (FOD) Model.

    abstract：:Abnormal filamentous aggregates that are formed by tangled tau protein turn out to be classic amyloid fibrils, meeting all the criteria defined under the fuzzy oil drop model in the context of amyloid characterization. The model recognizes amyloids as linear structures where local hydrophobicity minima and maxima prop...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms19102910

    authors： Dułak D,Gadzała M,Banach M,Ptak M,Wiśniowski Z,Konieczny L,Roterman I

    更新日期：2018-09-25 00:00:00

  • Inter-Species Differences in Regulation of the Progranulin-Sortilin Axis in TDP-43 Cell Models of Neurodegeneration.

    abstract：:Cytoplasmic aggregates and nuclear depletion of the ubiquitous RNA-binding protein TDP-43 have been described in the autoptic brain tissues of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTLD) patients and both TDP-43 loss-of-function and gain-of-function mechanisms seem to contribute to the neuro...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20235866

    authors： Gumina V,Onesto E,Colombrita C,Maraschi A,Silani V,Ratti A

    更新日期：2019-11-22 00:00:00

  • Silver Nanoparticles: Synthesis and Application for Nanomedicine.

    abstract：:Over the past few decades, metal nanoparticles less than 100 nm in diameter have made a substantial impact across diverse biomedical applications, such as diagnostic and medical devices, for personalized healthcare practice. In particular, silver nanoparticles (AgNPs) have great potential in a broad range of applicati...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms20040865

    authors： Lee SH,Jun BH

    更新日期：2019-02-17 00:00:00

  • Detection of Antigen-Specific T Cells Using In Situ MHC Tetramer Staining.

    abstract：:The development of in situ major histocompatibility complex (MHC) tetramer (IST) staining to detect antigen (Ag)-specific T cells in tissues has radically revolutionized our knowledge of the local cellular immune response to viral and bacterial infections, cancers, and autoimmunity. IST combined with immunohistochemis...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms20205165

    authors： Abdelaal HM,Cartwright EK,Skinner PJ

    更新日期：2019-10-18 00:00:00

  • Transcriptomics and Immunological Analyses Reveal a Pro-Angiogenic and Anti-Inflammatory Phenotype for Decidual Endothelial Cells.

    abstract：BACKGROUND:In pregnancy, excessive inflammation and break down of immunologic tolerance can contribute to miscarriage. Endothelial cells (ECs) are able to orchestrate the inflammatory processes by secreting pro-inflammatory mediators and bactericidal factors by modulating leakiness and leukocyte trafficking, via the ex...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20071604

    authors： Agostinis C,Masat E,Bossi F,Ricci G,Menegazzi R,Lombardelli L,Zito G,Mangogna A,Degan M,Gattei V,Piccinni MP,Kishore U,Bulla R

    更新日期：2019-03-31 00:00:00