Abstract:
:Microbial carbon degradation and methanogenesis in wetland soils generate a large proportion of atmospheric methane, a highly potent greenhouse gas. Despite their potential to mitigate greenhouse gas emissions, knowledge about methane-consuming methanotrophs is often limited to lower-resolution single-gene surveys that fail to capture the taxonomic and metabolic diversity of these microorganisms in soils. Here our objective was to use genome-enabled approaches to investigate methanotroph membership, distribution, and in situ activity across spatial and seasonal gradients in a freshwater wetland near Lake Erie. 16S rRNA gene analyses demonstrated that members of the methanotrophic Methylococcales were dominant, with the dominance largely driven by the relative abundance of four taxa, and enriched in oxic surface soils. Three methanotroph genomes from assembled soil metagenomes were assigned to the genus Methylobacter and represented the most abundant methanotrophs across the wetland. Paired metatranscriptomes confirmed that these Old Woman Creek (OWC) Methylobacter members accounted for nearly all the aerobic methanotrophic activity across two seasons. In addition to having the capacity to couple methane oxidation to aerobic respiration, these new genomes encoded denitrification potential that may sustain energy generation in soils with lower dissolved oxygen concentrations. We further show that Methylobacter members that were closely related to the OWC members were present in many other high-methane-emitting freshwater and soil sites, suggesting that this lineage could participate in methane consumption in analogous ecosystems. This work contributes to the growing body of research suggesting that Methylobacter may represent critical mediators of methane fluxes in freshwater saturated sediments and soils worldwide.IMPORTANCE Here we used soil metagenomics and metatranscriptomics to uncover novel members within the genus Methylobacter We denote these closely related genomes as members of the lineage OWC Methylobacter Despite the incredibly high microbial diversity in soils, here we present findings that unexpectedly showed that methane cycling was primarily mediated by a single genus for both methane production ("Candidatus Methanothrix paradoxum") and methane consumption (OWC Methylobacter). Metatranscriptomic analyses revealed that decreased methanotrophic activity rather than increased methanogenic activity possibly contributed to the greater methane emissions that we had previously observed in summer months, findings important for biogeochemical methane models. Although members of this Methylococcales order have been cultivated for decades, multi-omic approaches continue to illuminate the methanotroph phylogenetic and metabolic diversity harbored in terrestrial and marine ecosystems.
journal_name
mBiojournal_title
mBioauthors
Smith GJ,Angle JC,Solden LM,Borton MA,Morin TH,Daly RA,Johnston MD,Stefanik KC,Wolfe R,Gil B,Wrighton KCdoi
10.1128/mBio.00815-18subject
Has Abstractpub_date
2018-11-06 00:00:00issue
6issn
2150-7511pii
mBio.00815-18journal_volume
9pub_type
杂志文章相关文献
mBio文献大全abstract::The development of vaccines is one of the greatest medical interventions in the history of global infectious diseases and has contributed to the annual saving of at least 2 to 3 million lives worldwide. However, many diseases are not preventable through currently available vaccines, and the potential of modulating the...
journal_title:mBio
pub_type: 杂志文章,评审
doi:10.1128/mBio.02616-20
更新日期:2020-12-22 00:00:00
abstract:UNLABELLED:Particular types of hormonal contraceptives (HCs) and genital tract infections have been independently associated with risk of HIV-1 acquisition. We examined whether immunity in women using injectable depot medroxyprogesterone acetate (DMPA), combined oral contraceptives (COC), or no HCs differs by the prese...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00221-15
更新日期:2015-09-01 00:00:00
abstract:UNLABELLED:Fast, definitive diagnosis of Creutzfeldt-Jakob disease (CJD) is important in assessing patient care options and transmission risks. Real-time quaking-induced conversion (RT-QuIC) assays of cerebrospinal fluid (CSF) and nasal-brushing specimens are valuable in distinguishing CJD from non-CJD conditions but h...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02451-14
更新日期:2015-01-20 00:00:00
abstract::Aspergillus fumigatus is the leading cause of pulmonary fungal diseases. Azoles have been used for many years as the main antifungal agents to treat and prevent invasive aspergillosis. However, in the last 10 years there have been several reports of azole resistance in A. fumigatus and new strategies are needed to com...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00816-20
更新日期:2020-06-16 00:00:00
abstract:UNLABELLED:The human fungal pathogen Cryptococcus neoformans is capable of infecting a broad range of hosts, from invertebrates like amoebas and nematodes to standard vertebrate models such as mice and rabbits. Here we have taken advantage of a zebrafish model to investigate host-pathogen interactions of Cryptococcus w...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01425-15
更新日期:2015-09-29 00:00:00
abstract:UNLABELLED:The ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) pathogens cause an increasing number of nosocomial infections worldwide since they escape the inhibitory effect of the available antibiotics and the immun...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01842-15
更新日期:2016-01-26 00:00:00
abstract::Arthritogenic alphaviruses such as Ross River and Chikungunya viruses cause debilitating muscle and joint pain and pose significant challenges in the light of recent outbreaks. How host immune responses are orchestrated after alphaviral infections and lead to musculoskeletal inflammation remains poorly understood. Her...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.03353-19
更新日期:2020-03-03 00:00:00
abstract::Plasmodium vivax causes heavy burdens of disease across malarious regions worldwide. Mature P. vivax asexual and transmissive gametocyte stages occur in the blood circulation, and it is often assumed that accumulation/sequestration in tissues is not an important phase in their development. Here, we present a systemati...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00625-18
更新日期:2018-05-08 00:00:00
abstract:UNLABELLED:β-Lactam antibiotics are the drugs of choice to treat pneumococcal infections. The spread of β-lactam-resistant pneumococci is a major concern in choosing an effective therapy for patients. Systematically tracking β-lactam resistance could benefit disease surveillance. Here we developed a classification syst...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00756-16
更新日期:2016-06-14 00:00:00
abstract::Paramyxoviruses are negative-sense single-stranded RNA viruses that comprise many important human and animal pathogens, including human parainfluenza viruses. These viruses bud from the plasma membrane of infected cells after the viral ribonucleoprotein complex (vRNP) is transported from the cytoplasm to the cell memb...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02028-20
更新日期:2020-08-25 00:00:00
abstract:UNLABELLED:Human noroviruses (HuNoVs) cause significant morbidity and mortality worldwide. However, despite substantial efforts, a small-animal model for HuNoV has not been described to date. Since "humanized" mice have been successfully used to study human-tropic pathogens in the past, we challenged BALB/c mice defici...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00450-13
更新日期:2013-07-16 00:00:00
abstract::Bacteroides thetaiotaomicron is one of the most abundant gut symbiont species, whose contribution to host health through its ability to degrade dietary polysaccharides and mature the immune system is under intense scrutiny. In contrast, adhesion and biofilm formation, which are potentially involved in gut colonization...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00729-20
更新日期:2020-06-23 00:00:00
abstract::The Middle East respiratory syndrome (MERS) is proposed to be a zoonotic disease; however, the reservoir and mechanism for transmission of the causative agent, the MERS coronavirus, are unknown. Dromedary camels have been implicated through reports that some victims have been exposed to camels, camels in areas where t...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00884-14
更新日期:2014-02-25 00:00:00
abstract:UNLABELLED:Curing HIV-1 infection will require elimination of persistent cellular reservoirs that harbor latent virus in the face of combination antiretroviral therapy (cART). Proposed immunotherapeutic strategies to cure HIV-1 infection include enhancing lysis of these infected cells by cytotoxic T lymphocytes (CTL). ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00473-16
更新日期:2016-05-31 00:00:00
abstract::The interplay between translation initiation, modification of translation initiation factors, and selection of start sites on mRNA for protein synthesis can play a regulatory role in the cellular response to stress, development, and cell fate in eukaryotic species by shaping the proteome. As shown by Ivanov et al. (mB...
journal_title:mBio
pub_type: 评论,杂志文章
doi:10.1128/mBio.01820-17
更新日期:2017-11-07 00:00:00
abstract:UNLABELLED:Elevated intracellular levels of the bacterial second messenger c-di-GMP are known to suppress motility and promote sessility. Bacterial chemotaxis guides motile cells in gradients of attractants and repellents over broad concentration ranges, thus allowing bacteria to quickly adapt to changes in their surro...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00001-13
更新日期:2013-03-19 00:00:00
abstract::In Escherichia coli, FtsEX coordinates peptidoglycan (PG) synthesis and hydrolysis at the septum. It acts on FtsA in the cytoplasm to promote recruitment of septal PG synthetases and recruits EnvC, an activator of septal PG hydrolases, in the periplasm. Following recruitment, ATP hydrolysis by FtsEX is thought to regu...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01247-20
更新日期:2020-07-07 00:00:00
abstract:UNLABELLED:The current tuberculosis (TB) vaccine, Mycobacterium bovis Bacillus Calmette-Guérin (BCG), provides insufficient protection against pulmonary TB. Previously, we generated a listeriolysin-expressing recombinant BCG strain, which to date has successfully completed phase I and phase IIa clinical trials. In an a...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00679-16
更新日期:2016-05-24 00:00:00
abstract::The human malaria parasite Plasmodium falciparum replicates within circulating red blood cells, where it is subjected to conditions that frequently cause DNA damage. The repair of DNA double-stranded breaks (DSBs) is thought to rely almost exclusively on homologous recombination (HR), due to a lack of efficient nonhom...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00547-17
更新日期:2017-08-08 00:00:00
abstract::E165R, a highly specific dUTP nucleotidohydrolase (dUTPase) encoded by the African swine fever virus (ASFV) genome, is required for productive replication of ASFV in swine macrophages. Here, we solved the high-resolution crystal structures of E165R in its apo state and in complex with its product dUMP. Structural anal...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02483-19
更新日期:2019-10-29 00:00:00
abstract:UNLABELLED:Acinetobacter baumannii is a nosocomial pathogen of increasing importance due to its multiple resistance to antibiotics and ability to survive in the hospital environment linked to its capacity to form biofilms. To fully characterize the contribution of AdeABC, AdeFGH, and AdeIJK resistance-nodulation-cell d...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00309-15
更新日期:2015-03-24 00:00:00
abstract::As the GroES/GroEL chaperonin system is the only bacterial chaperone that is essential under all conditions, we have been interested in the development of GroES/GroEL inhibitors as potential antibiotics. Using Escherichia coli GroES/GroEL as a surrogate, we have discovered several classes of GroES/GroEL inhibitors tha...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02167-20
更新日期:2021-01-12 00:00:00
abstract::Staphylococcus aureus is intrinsically resistant to polymyxins (polymyxin B and colistin), an important class of cationic antimicrobial peptides used in treatment of Gram-negative bacterial infections. To understand the mechanisms underlying intrinsic polymyxin resistance in S. aureus, we screened the Nebraska Transpo...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01114-17
更新日期:2017-09-05 00:00:00
abstract:UNLABELLED:Kaposi sarcoma (KS) herpesvirus (KSHV) infection of endothelial cells (EC) is associated with strong induction of heme oxygenase-1 (HO-1), a stress-inducible host gene that encodes the rate-limiting enzyme responsible for heme catabolism. KS is an angioproliferative tumor characterized by the proliferation o...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00668-15
更新日期:2015-06-04 00:00:00
abstract:UNLABELLED:Exploiting mechanisms of utilizing the sugar d-galactose in Escherichia coli as a model system, we explored the consequences of accumulation of critical intermediates of the d-galactose metabolic pathways by monitoring cell growth, metabolites, and transcript profiles. These studies revealed both metabolic n...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00972-13
更新日期:2014-01-28 00:00:00
abstract::Bacteria of the phylum Bacteroidetes, including commensal organisms and opportunistic pathogens, harbor abundant surface-exposed multiprotein membrane complexes (Sus-like systems) involved in carbohydrate acquisition. These complexes have been mostly linked to commensalism, and in some instances, they have also been s...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01232-16
更新日期:2016-10-25 00:00:00
abstract::The genetic and biochemical basis of perchlorate-dependent H2S oxidation (PSOX) was investigated in the dissimilatory perchlorate-reducing microorganism (DPRM) Azospira suillum PS (PS). Previously, it was shown that all known DPRMs innately oxidize H2S, producing elemental sulfur (So). Although the process involving P...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02023-16
更新日期:2017-02-21 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) Tat binds the viral RNA structure transactivation-responsive element (TAR) and recruits transcriptional cofactors, amplifying viral mRNA expression. The Tat inhibitor didehydro-cortistatin A (dCA) promotes a state of persistent latency, refractory to viral reactivation. Here...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01750-18
更新日期:2019-07-02 00:00:00
abstract::Infection of host cells by the respiratory syncytial virus (RSV) is characterized by the formation of spherical cytoplasmic inclusion bodies (IBs). These structures, which concentrate all the proteins of the polymerase complex as well as some cellular proteins, were initially considered aggresomes formed by viral dead...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01202-20
更新日期:2020-09-22 00:00:00
abstract::The acquisition and incorporation of genetic material between nonmating species, or horizontal gene transfer (HGT), has been frequently described for phylogenetically related organisms, but far less evidence exists for HGT between highly divergent organisms. Here we report the identification and characterization of a ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00005-11
更新日期:2011-02-15 00:00:00