Abstract:
:In Escherichia coli, FtsEX coordinates peptidoglycan (PG) synthesis and hydrolysis at the septum. It acts on FtsA in the cytoplasm to promote recruitment of septal PG synthetases and recruits EnvC, an activator of septal PG hydrolases, in the periplasm. Following recruitment, ATP hydrolysis by FtsEX is thought to regulate both PG synthesis and hydrolysis, but how it does this is not well understood. Here, we show that an ATPase mutant of FtsEX blocks septal PG synthesis similarly to cephalexin, suggesting that ATP hydrolysis by FtsEX is required throughout septation. Using mutants that uncouple the roles of FtsEX in septal PG synthesis and hydrolysis, we find that recruitment of EnvC to the septum by FtsEX, but not ATP hydrolysis, is required to promote cell separation when the NlpD-mediated cell separation system is present. However, ATP hydrolysis by FtsEX becomes necessary for efficient cell separation when the NlpD system is inactivated, suggesting that the ATPase activity of FtsEX is required for optimal activity of EnvC. Importantly, under conditions that suppress the role of FtsEX in cell division, disruption of the FtsEX-FtsA interaction delays cell separation, highlighting the importance of this interaction in coupling the cell separation system with the septal PG synthetic complex.IMPORTANCE Cytokinesis in Gram-negative bacteria requires coordinated invagination of the three layers of the cell envelope; otherwise, cells become sensitive to hydrophobic antibiotics and can even undergo cell lysis. InE. coli, the ABC transporter FtsEX couples the synthesis and hydrolysis of the stress-bearing peptidoglycan layer at the septum by interacting with FtsA and EnvC, respectively. ATP hydrolysis by FtsEX is critical for its function, but the reason why is not clear. Here, we find that in the absence of ATP hydrolysis, FtsEX blocks septal PG synthesis similarly to cephalexin. However, an FtsEX ATPase mutant, under conditions where it cannot block division, rescues ftsEX phenotypes as long as a partially redundant cell separation system is present. Furthermore, we find that the FtsEX-FtsA interaction is important for efficient cell separation.
journal_name
mBiojournal_title
mBioauthors
Du S,Pichoff S,Lutkenhaus Jdoi
10.1128/mBio.01247-20subject
Has Abstractpub_date
2020-07-07 00:00:00issue
4issn
2150-7511pii
mBio.01247-20journal_volume
11pub_type
杂志文章相关文献
mBio文献大全abstract::Butanol production by Clostridium acetobutylicum is accompanied by coproduction of acetone and ethanol, which reduces the yield of butanol and increases the production cost. Here, we report development of several clostridial aldehyde/alcohol dehydrogenase (AAD) variants showing increased butanol selectivity by a serie...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02683-18
更新日期:2019-01-22 00:00:00
abstract::Although the pathogen recognition receptor pathways that activate cell-intrinsic antiviral responses are well delineated, less is known about how the host regulates this response to prevent sustained signaling and possible immune-mediated damage. Using a genome-wide CRISPR-Cas9 screening approach to identify host fact...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00136-20
更新日期:2020-03-10 00:00:00
abstract::Different members of the Mycobacterium genus have evolved to cause tuberculosis in diverse human populations and in a variety of animal species. Our cumulative knowledge of mycobacterial genomes indicates that mutations in the PhoPR two-component virulence system were acquired not only during the natural evolution of ...
journal_title:mBio
pub_type: 杂志文章,评审
doi:10.1128/mBio.01289-15
更新日期:2015-10-20 00:00:00
abstract::The saw-toothed grain beetle, Oryzaephilus surinamensis (Silvanidae), is a cosmopolitan stored-product pest. Early studies on O. surinamensis in the 1930s described the presence of peculiar bacteriomes harboring endosymbiotic bacteria in the abdomen. Since then, however, the microbiological nature of the symbiont has ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01482-17
更新日期:2017-09-26 00:00:00
abstract::Mucormycosis is an emerging lethal fungal infection in immunocompromised patients. Mucor circinelloides is a causal agent of mucormycosis and serves as a model system to understand genetics in Mucorales. Calcineurin is a conserved virulence factor in many pathogenic fungi, and calcineurin inhibition or deletion of the...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02949-19
更新日期:2020-01-28 00:00:00
abstract::Estrogen, a major female sex steroid hormone, has been shown to promote the selection of mucoid Pseudomonas aeruginosa in the airways of patients with chronic respiratory diseases, including cystic fibrosis. This results in long-term persistence, poorer clinical outcomes, and limited therapeutic options. In this study...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01774-20
更新日期:2020-09-29 00:00:00
abstract::Staphylococcus aureus is intrinsically resistant to polymyxins (polymyxin B and colistin), an important class of cationic antimicrobial peptides used in treatment of Gram-negative bacterial infections. To understand the mechanisms underlying intrinsic polymyxin resistance in S. aureus, we screened the Nebraska Transpo...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01114-17
更新日期:2017-09-05 00:00:00
abstract::Candida albicans has developmental programs that govern transitions between yeast and filamentous morphologies and between unattached and biofilm lifestyles. Here, we report that filamentation, intercellular adherence, and biofilm development were inhibited during interactions between Candida albicans and Pseudomonas ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00526-12
更新日期:2013-01-29 00:00:00
abstract::Resistance-nodulation-division (RND) superfamily efflux systems have been widely studied for their role in antibiotic resistance, but their native biological functions remain poorly understood. We previously showed that loss of RND-mediated efflux in Vibrio cholerae resulted in activation of the Cpx two-component regu...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00126-17
更新日期:2017-05-16 00:00:00
abstract::Gammaherpesviruses (γHVs) are generally considered host specific and to have codiverged with their hosts over millions of years. This tenet is challenged here by broad-scale phylogenetic analysis of two viral genes using the largest sample of mammalian γHVs to date, integrating for the first time bat γHV sequences ava...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01425-16
更新日期:2016-11-08 00:00:00
abstract:UNLABELLED:Osteomyelitis is a difficult-to-eradicate bone infection typically caused by Staphylococcus aureus. In this study, we investigated the in vivo transcriptional adaptation of S. aureus during bone infection. To this end, we determined the transcriptome of S. aureus during the acute (day 7) and chronic (day 28)...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01775-14
更新日期:2014-12-23 00:00:00
abstract::Streptococcus gallolyticus subsp. gallolyticus is an emerging opportunistic pathogen responsible for septicemia and endocarditis in the elderly. Invasive infections by S. gallolyticus subsp. gallolyticus are strongly linked to the occurrence of colorectal cancer (CRC). It was previously shown that increased secondary ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.03189-20
更新日期:2021-01-05 00:00:00
abstract::Pulmonary exacerbations are the leading cause of death in cystic fibrosis (CF) patients. To track microbial dynamics during acute exacerbations, a CF rapid response (CFRR) strategy was developed. The CFRR relies on viromics, metagenomics, metatranscriptomics, and metabolomics data to rapidly monitor active members of ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00431-19
更新日期:2019-04-16 00:00:00
abstract::The rapid increase of carbapenem resistance in Gram-negative bacteria has resurrected the importance of the polymyxin antibiotics. The recent discovery of plasmid-mediated polymyxin resistance (mcr-1) in carbapenem-resistant Enterobacteriaceae serves as an important indicator that the golden era of antibiotics is unde...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00540-17
更新日期:2017-07-25 00:00:00
abstract::An operon comprising two genes, CA_P0037 and CA_P0036, that encode proteins of unknown function that were previously shown to be highly expressed in acidogenic cells and repressed in solventogenic and alcohologenic cells is located on the pSOL1 megaplasmid of Clostridium acetobutylicum upstream of adhE2 A CA_P0037::in...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01218-16
更新日期:2016-10-04 00:00:00
abstract::Emerging zoonotic viral diseases remain a challenge to global public health. Recent surveillance studies have implicated bats as potential reservoirs for a number of viral pathogens, including coronaviruses and Ebola viruses. Caliciviridae represent a major viral family contributing to emerging diseases in both human ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00869-18
更新日期:2018-05-22 00:00:00
abstract::Bacillus anthracis is a Gram-positive bacillus that under conditions of environmental stress, such as low nutrients, can convert from a vegetative bacillus to a highly durable spore that enables long-term survival. The sporulation process is regulated by a sequential cascade of dedicated transcription factors but requ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02079-18
更新日期:2018-11-06 00:00:00
abstract::Zika virus is a mosquito-borne flavivirus which can cause severe disease in humans, including microcephaly and other congenital malformations in newborns and Guillain-Barré syndrome in adults. There are currently no approved prophylactics or therapeutics for Zika virus; the development of a safe and effective vaccine ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02861-18
更新日期:2019-04-02 00:00:00
abstract::Epsilon toxin (ETX), a pore-forming toxin produced by type B and D strains of Clostridium perfringens, mediates severe enterotoxemia in livestock and possibly plays a role in human disease. During enterotoxemia, the nearly inactive ETX prototoxin is produced in the intestines but then must be activated by proteolytic ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01994-14
更新日期:2014-10-21 00:00:00
abstract::Cellular sensing of bacterial RNA is increasingly recognized as a determinant of host-pathogen interactions. The intracellular pathogen Listeria monocytogenes induces high levels of type I interferons (alpha/beta interferons [IFN-α/β]) to create a growth-permissive microenvironment during infection. We previously demo...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01223-19
更新日期:2019-10-08 00:00:00
abstract:UNLABELLED:Sensing of and responding to environmental changes are of vital importance for microbial cells. Consequently, bacteria have evolved a plethora of signaling systems that usually sense biochemical cues either via direct ligand binding, thereby acting as "concentration sensors," or by responding to downstream e...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00975-15
更新日期:2015-07-21 00:00:00
abstract::Heme oxygenase-1 (HO-1) is a stress response antioxidant enzyme which catalyzes the degradation of heme released during inflammation. HO-1 expression is upregulated in both experimental and human Mycobacterium tuberculosis infection, and in patients it is a biomarker of active disease. Whether the enzyme plays a prote...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01675-16
更新日期:2016-10-25 00:00:00
abstract:UNLABELLED:The Pseudomonas aeruginosa type III secretion system has been associated with poor outcomes in both animal models and human patients. Despite a large number of studies exploring the regulation of type III secretion in vitro, little is known about the timing of secretion during mammalian infection. Here we de...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00032-13
更新日期:2013-03-12 00:00:00
abstract::The PCNA (proliferating cell nuclear antigen) ring plays central roles during DNA replication and repair. The yeast Elg1 RFC-like complex (RLC) is the principal unloader of chromatin-bound PCNA and thus plays a central role in maintaining genome stability. Here we identify a role for Elg1 in the unloading of PCNA duri...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01159-19
更新日期:2019-06-11 00:00:00
abstract::Photosynthetic bacteria are capable of producing their own food via photosynthesis. Unsurprisingly, they evolved the ability to move toward better light conditions (i.e., phototaxis). In a recent article in mBio, Chau et al. tuned the wavelength, flux, direction, and timing of light input and characterized the motilit...
journal_title:mBio
pub_type: 评论,杂志文章
doi:10.1128/mBio.00498-17
更新日期:2017-04-11 00:00:00
abstract::Membrane repair emerges as an innate defense protecting target cells against bacterial pore-forming toxins. Here, we report the first paradigm of Ca2+-dependent repair following attack by a small β-pore-forming toxin, namely, plasmid-encoded phobalysin of Photobacterium damselae subsp. damselae In striking contrast, V...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02083-16
更新日期:2017-02-14 00:00:00
abstract::DNA supercoiling (DS) is essential for life because it controls critical processes, including transcription, replication, and recombination. Current methods to measure DNA supercoiling in vivo are laborious and unable to examine single cells. Here, we report a method for high-throughput measurement of bacterial DNA su...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01053-20
更新日期:2020-07-28 00:00:00
abstract::The biosynthesis of antioxidant pigments, namely, betalains, was believed to be restricted to Caryophyllales plants. This paper changes this paradigm, and enzyme mining from bacterial hosts promoted the discovery of bacterial cultures producing betalains. The spectrum of possible sources of betalain pigments in nature...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00345-19
更新日期:2019-03-19 00:00:00
abstract::The availability of energy has significant impact on cell physiology. However, the role of cellular metabolism in bacterial pathogenesis is not understood. We investigated the dynamics of central metabolism during virulence induction by surface sensing and quorum sensing in early-stage biofilms of the multidrug-resist...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02730-18
更新日期:2020-03-10 00:00:00
abstract::Staphylococcus aureus is a human pathogen responsible for high morbidity and mortality worldwide. Recurrent infections with this bacterium are common, suggesting that S. aureus thwarts the development of sterilizing immunity. S. aureus strains that cause disease in humans produce up to five different bicomponent toxin...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01918-18
更新日期:2019-01-02 00:00:00