Abstract:
:The PCNA (proliferating cell nuclear antigen) ring plays central roles during DNA replication and repair. The yeast Elg1 RFC-like complex (RLC) is the principal unloader of chromatin-bound PCNA and thus plays a central role in maintaining genome stability. Here we identify a role for Elg1 in the unloading of PCNA during DNA damage. Using DNA damage checkpoint (DC)-inducible and replication checkpoint (RC)-inducible strains, we show that Elg1 is essential for eliciting the signal in the DC branch. In the absence of Elg1 activity, the Rad9 (53BP1) and Dpb11 (TopBP1) adaptor proteins are recruited but fail to be phosphorylated by Mec1 (ATR), resulting in a lack of checkpoint activation. The chromatin immunoprecipitation of PCNA at the Lac operator sites reveals that accumulated local PCNA influences the checkpoint activation process in elg1 mutants. Our data suggest that Elg1 participates in a mechanism that may coordinate PCNA unloading during DNA repair with DNA damage checkpoint induction.IMPORTANCE The Elg1protein forms an RFC-like complex in charge of unloading PCNA from chromatin during DNA replication and repair. Mutations in the ELG1 gene caused genomic instability in all organisms tested and cancer in mammals. Here we show that Elg1 plays a role in the induction of the DNA damage checkpoint, a cellular response to DNA damage. We show that this defect is due to a defect in the signal amplification process during induction. Thus, cells coordinate the cell's response and the PCNA unloading through the activity of Elg1.
journal_name
mBiojournal_title
mBioauthors
Sau S,Liefshitz B,Kupiec Mdoi
10.1128/mBio.01159-19subject
Has Abstractpub_date
2019-06-11 00:00:00issue
3issn
2150-7511pii
mBio.01159-19journal_volume
10pub_type
杂志文章相关文献
mBio文献大全abstract:UNLABELLED:Elevated intracellular levels of the bacterial second messenger c-di-GMP are known to suppress motility and promote sessility. Bacterial chemotaxis guides motile cells in gradients of attractants and repellents over broad concentration ranges, thus allowing bacteria to quickly adapt to changes in their surro...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00001-13
更新日期:2013-03-19 00:00:00
abstract:UNLABELLED:Sensing of and responding to environmental changes are of vital importance for microbial cells. Consequently, bacteria have evolved a plethora of signaling systems that usually sense biochemical cues either via direct ligand binding, thereby acting as "concentration sensors," or by responding to downstream e...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00975-15
更新日期:2015-07-21 00:00:00
abstract::Although the TEM-1 β-lactamase (BlaTEM-1) hydrolyzes penicillins and narrow-spectrum cephalosporins, organisms expressing this enzyme are typically susceptible to β-lactam/β-lactamase inhibitor combinations such as piperacillin-tazobactam (TZP). However, our previous work led to the discovery of 28 clinical isolates o...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00583-18
更新日期:2018-04-24 00:00:00
abstract::Infection of host cells by the respiratory syncytial virus (RSV) is characterized by the formation of spherical cytoplasmic inclusion bodies (IBs). These structures, which concentrate all the proteins of the polymerase complex as well as some cellular proteins, were initially considered aggresomes formed by viral dead...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01202-20
更新日期:2020-09-22 00:00:00
abstract::Proteins that metabolize or bind the nucleotide second messenger cyclic diguanylate regulate a wide variety of important processes in bacteria. These processes include motility, biofilm formation, cell division, differentiation, and virulence. The role of cyclic diguanylate signaling in the lifestyle of Legionella pne...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00316-10
更新日期:2011-01-11 00:00:00
abstract::Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of three subunits, namely, NF-YA, NF-YB, and NF-YC, which are conserved throughout evolution. In higher eukaryotes, NF-Y plays important roles in several cellular processes (development, cell cycle regulation, apoptosis, and response to growth, ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00737-19
更新日期:2019-06-18 00:00:00
abstract::Epidemiological studies on Streptococcus pneumoniae show that rates of carriage are highest in early childhood and that the major benefit of the pneumococcal conjugate vaccine (PCV) is a reduction in the incidence of nasopharyngeal colonization through decreased transmission within a population. In this study, we soug...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00188-17
更新日期:2017-03-14 00:00:00
abstract::Misincorporation of uracil or spontaneous cytidine deamination is a common mutagenic insult to DNA. Herpesviruses encode a viral uracil-DNA glycosylase (vUNG) and a viral dUTPase (vDUT), each with enzymatic and nonenzymatic functions. However, the coordinated roles of these enzymatic activities in gammaherpesvirus pat...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01831-18
更新日期:2018-10-30 00:00:00
abstract:UNLABELLED:Rarely, if ever, has a single bacterial cell been confirmed to simultaneously host two fundamentally different predators. Two such predators are viruses and the predatory prokaryotes known as Bdellovibrio and like organisms. Viruses or bacteriophage are particles requiring prey cells in an active metabolic s...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00051-12
更新日期:2012-04-17 00:00:00
abstract::Yellow fever virus (YFV) is an arthropod-borne flavivirus, infecting ~200,000 people worldwide annually and causing about 30,000 deaths. The live attenuated vaccine strain, YFV-17D, has significantly contributed in controlling the global burden of yellow fever worldwide. However, the viral and host contributions to YF...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00819-17
更新日期:2017-08-15 00:00:00
abstract:UNLABELLED:In the model eukaryote Saccharomyces cerevisiae, the transcription factor Cst6p has been reported to play important roles in several biological processes. However, the genome-wide targets of Cst6p and its physiological functions remain unknown. Here, we mapped the genome-wide binding sites of Cst6p at high r...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00559-16
更新日期:2016-05-03 00:00:00
abstract:UNLABELLED:A rapid and global emergence of azole resistance has been observed in the pathogenic fungus Aspergillus fumigatus over the past decade. The dominant resistance mechanism appears to be of environmental origin and involves mutations in the cyp51A gene, which encodes a protein targeted by triazole antifungal dr...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00536-15
更新日期:2015-06-02 00:00:00
abstract:UNLABELLED:Cholera continues to be a global threat, with high rates of morbidity and mortality. In 2011, a cholera outbreak occurred in Palawan, Philippines, affecting more than 500 people, and 20 individuals died. Vibrio cholerae O1 was confirmed as the etiological agent. Source attribution is critical in cholera outb...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00047-15
更新日期:2015-04-21 00:00:00
abstract:UNLABELLED:Rapidly fatal cases of invasive fungal infections due to a fungus later identified as Saprochaete clavata were reported in France in May 2012. The objectives of this study were to determine the clonal relatedness of the isolates and to investigate possible sources of contamination. A nationwide alert was lau...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02309-14
更新日期:2014-12-16 00:00:00
abstract::The cholesterol-dependent cytolysins (CDCs) are bacterial, β-barrel, pore-forming toxins. A central enigma of the pore-forming mechanism is how completion of the prepore is sensed to initiate its conversion to the pore. We identified a motif that is conserved between the CDCs and a diverse family of nearly 300 unchara...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02351-20
更新日期:2020-09-29 00:00:00
abstract::Cell surface expression of sialic acid has been reported to decrease during immune cell activation, but the significance and regulation of this phenomenon are still being investigated. The major human bacterial pathogen Streptococcus pneumoniae causes pneumonia, sepsis and meningitis, often accompanied by strong infla...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00220-11
更新日期:2012-01-03 00:00:00
abstract:UNLABELLED:Competence for genetic transformation in Streptococcus pneumoniae develops in response to accumulation of a secreted peptide pheromone and was one of the initial examples of bacterial quorum sensing. Activation of this signaling system induces not only expression of the proteins required for transformation b...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00071-11
更新日期:2011-09-20 00:00:00
abstract::In bacteria, elongation factor Tu is a translational cofactor that forms ternary complexes with aminoacyl-tRNA (aa-tRNA) and GTP. Binding of a ternary complex to one of four flexible L7/L12 units on the ribosome tethers a charged tRNA in close proximity to the ribosomal A site. Two sequential tests for a match between...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02143-17
更新日期:2018-01-16 00:00:00
abstract:UNLABELLED:Enterococcus faecium, natively a gut commensal organism, emerged as a leading cause of multidrug-resistant hospital-acquired infection in the 1980s. As the living record of its adaptation to changes in habitat, we sequenced the genomes of 51 strains, isolated from various ecological environments, to understa...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00534-13
更新日期:2013-08-20 00:00:00
abstract::Low doses of antibiotics can trigger secondary metabolite biosynthesis in bacteria, but the underlying mechanisms are generally unknown. We sought to better understand this phenomenon by studying how the antibiotic trimethoprim activates the synthesis of the virulence factor malleilactone in Burkholderia thailandensis...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.03210-19
更新日期:2020-02-25 00:00:00
abstract::The intracellular parasite Toxoplasma gondii employs a vast array of effector proteins from the rhoptry and dense granule organelles to modulate host cell biology; these effectors are known as ROPs and GRAs, respectively. To examine the individual impacts of ROPs and GRAs on host gene expression, we developed a robust...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00182-20
更新日期:2020-06-09 00:00:00
abstract::Malaria parasites have a complex life cycle with multiple developmental stages in mosquito and vertebrate hosts, and different developmental stages express unique sets of genes. Unexpectedly, many transcription factors (TFs) commonly found in eukaryotic organisms are absent in malaria parasites; instead, a family of g...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01986-17
更新日期:2017-12-12 00:00:00
abstract::Malaria parasite ookinetes must traverse the vector mosquito midgut epithelium to transform into sporozoite-producing oocysts. The Anopheles innate immune system is a key regulator of this process, thereby determining vector competence and disease transmission. The role of Anopheles innate immunity factors as agonists...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01631-17
更新日期:2017-10-17 00:00:00
abstract::Transposon insertion sequencing (TIS) is a powerful high-throughput genetic technique that is transforming functional genomics in prokaryotes, because it enables genome-wide mapping of the determinants of fitness. However, current approaches for analyzing TIS data assume that selective pressures are constant over time...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01581-17
更新日期:2017-10-03 00:00:00
abstract::Many Gram-negative bacterial pathogens require a type 3 secretion system (T3SS) to deliver effector proteins into eukaryotic cells. Contact of the tip complex of the T3SS with a target eukaryotic cell initiates secretion of the two bacterial proteins that assemble into the translocon pore in the plasma membrane. The t...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00877-19
更新日期:2019-05-28 00:00:00
abstract::The conformation of the HIV-1 envelope glycoprotein (Env) substantially impacts antibody recognition and antibody-dependent cellular cytotoxicity (ADCC) responses. In the absence of the CD4 receptor at the cell surface, primary Envs sample a "closed" conformation that occludes CD4-induced (CD4i) epitopes. The virus co...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00358-18
更新日期:2018-03-20 00:00:00
abstract::Tuberculosis (TB), caused by Mycobacterium tuberculosis infection, remains a major cause of mortality and morbidity worldwide. One-third of the world population is infected with M. tuberculosis, and about 15 million people with latent tuberculosis infection (LTBI) reside in the United States. An estimated 10% of indiv...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02771-18
更新日期:2019-04-16 00:00:00
abstract:UNLABELLED:The newly emerging Middle East respiratory syndrome coronavirus (MERS-CoV) causes a severe respiratory infection with a high mortality rate (~35%). MERS-CoV has been a global threat due to continuous outbreaks in the Arabian peninsula and international spread by infected travelers since 2012. From May to Jul...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00019-16
更新日期:2016-03-01 00:00:00
abstract:UNLABELLED:Human immunodeficiency virus type 1 (HIV-1) Nef enhances virus replication and contributes to immune evasion in vivo, but the underlying molecular mechanisms remain incompletely defined. Nef interferes with host cell actin dynamics to restrict T lymphocyte responses to chemokine stimulation and T cell recept...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01309-15
更新日期:2015-09-08 00:00:00
abstract::The diversity of the genetic code systems used by microbes on earth is yet to be elucidated. It is known that certain methanogenic archaea employ an alternative system for cysteine (Cys) biosynthesis and encoding; tRNACys is first acylated with phosphoserine (Sep) by O-phosphoseryl-tRNA synthetase (SepRS) and then con...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00561-17
更新日期:2017-05-09 00:00:00