Abstract:
:The genetic and biochemical basis of perchlorate-dependent H2S oxidation (PSOX) was investigated in the dissimilatory perchlorate-reducing microorganism (DPRM) Azospira suillum PS (PS). Previously, it was shown that all known DPRMs innately oxidize H2S, producing elemental sulfur (So). Although the process involving PSOX is thermodynamically favorable (ΔG°' = -206 kJ ⋅ mol-1 H2S), the underlying biochemical and genetic mechanisms are currently unknown. Interestingly, H2S is preferentially utilized over physiological electron donors such as lactate or acetate although no growth benefit is obtained from the metabolism. Here, we determined that PSOX is due to a combination of enzymatic and abiotic interactions involving reactive intermediates of perchlorate respiration. Using various approaches, including barcode analysis by sequencing (Bar-seq), transcriptome sequencing (RNA-seq), and proteomics, along with targeted mutagenesis and biochemical characterization, we identified all facets of PSOX in PS. In support of our proposed model, deletion of identified upregulated PS genes traditionally known to be involved in sulfur redox cycling (e.g., Sox, sulfide:quinone reductase [SQR]) showed no defect in PSOX activity. Proteomic analysis revealed differential abundances of a variety of stress response metal efflux pumps and divalent heavy-metal transporter proteins, suggesting a general toxicity response. Furthermore, in vitro biochemical studies demonstrated direct PSOX mediated by purified perchlorate reductase (PcrAB) in the absence of other electron transfer proteins. The results of these studies support a model in which H2S oxidation is mediated by electron transport chain short-circuiting in the periplasmic space where the PcrAB directly oxidizes H2S to So The biogenically formed reactive intermediates (ClO2- and O2) subsequently react with additional H2S, producing polysulfide and So as end products.IMPORTANCE Inorganic sulfur compounds are widespread in nature, and microorganisms are central to their transformation, thereby playing a key role in the global sulfur cycle. Sulfur oxidation is mediated by a broad phylogenetic diversity of microorganisms, including anoxygenic phototrophs and either aerobic or anaerobic chemotrophs coupled to oxygen or nitrate respiration, respectively. Recently, perchlorate-respiring microorganisms were demonstrated to be innately capable of sulfur oxidation regardless of their phylogenetic affiliation. As recognition of the prevalence of these organisms intensifies, their role in global geochemical cycles is being queried. This is further highlighted by the recently recognized environmental pervasiveness of perchlorate not only across Earth but also throughout our solar system. The inferred importance of this metabolism not only is that it is a novel and previously unrecognized component of the global sulfur redox cycle but also is because of the recently demonstrated applicability of perchlorate respiration in the control of biogenic sulfide production in engineered environments such as oil reservoirs and wastewater treatment facilities, where excess H2S represents a significant environmental, process, and health risk, with associated costs approximating $90 billion annually.
journal_name
mBiojournal_title
mBioauthors
Mehta-Kolte MG,Loutey D,Wang O,Youngblut MD,Hubbard CG,Wetmore KM,Conrad ME,Coates JDdoi
10.1128/mBio.02023-16subject
Has Abstractpub_date
2017-02-21 00:00:00issue
1issn
2150-7511pii
mBio.02023-16journal_volume
8pub_type
杂志文章相关文献
mBio文献大全abstract:UNLABELLED:Pasteurella multocida toxin (PMT) induces atrophic rhinitis in animals, which is characterized by a degradation of nasal turbinate bones, indicating an effect of the toxin on bone cells such as osteoblasts and osteoclasts. The underlying molecular mechanism of PMT was defined as a persistent activation of he...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02190-14
更新日期:2014-11-11 00:00:00
abstract::The availability of energy has significant impact on cell physiology. However, the role of cellular metabolism in bacterial pathogenesis is not understood. We investigated the dynamics of central metabolism during virulence induction by surface sensing and quorum sensing in early-stage biofilms of the multidrug-resist...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02730-18
更新日期:2020-03-10 00:00:00
abstract::New drugs are needed to control the current tuberculosis (TB) pandemic caused by infection with Mycobacterium tuberculosis We report here on our work with AX-35, an arylvinylpiperazine amide, and four related analogs, which are potent antitubercular agents in vitro All five compounds showed good activity against M. tu...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01276-18
更新日期:2018-10-09 00:00:00
abstract:UNLABELLED:A 32-nucleotide (nt) RNA motif located at the 3' end of the transmissible gastroenteritis coronavirus (TGEV) genome was found to specifically interact with the host proteins glutamyl-prolyl-tRNA synthetase (EPRS) and arginyl-tRNA synthetase (RRS). This RNA motif has high homology in sequence and secondary st...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00105-15
更新日期:2015-03-10 00:00:00
abstract::Listeria monocytogenes is a bacterial pathogen causing severe foodborne infections in humans and animals. Listeria can enter into host cells and survive and multiply therein, due to an arsenal of virulence determinants encoded in different loci on the chromosome. Several key Listeria virulence genes are clustered in L...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01550-17
更新日期:2017-10-31 00:00:00
abstract::The flagellar motor can spin in both counterclockwise (CCW) and clockwise (CW) directions. The flagellar motor consists of a rotor and multiple stator units, which act as a proton channel. The rotor is composed of the transmembrane MS ring made of FliF and the cytoplasmic C ring consisting of FliG, FliM, and FliN. The...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00079-19
更新日期:2019-04-02 00:00:00
abstract::Cell surface expression of sialic acid has been reported to decrease during immune cell activation, but the significance and regulation of this phenomenon are still being investigated. The major human bacterial pathogen Streptococcus pneumoniae causes pneumonia, sepsis and meningitis, often accompanied by strong infla...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00220-11
更新日期:2012-01-03 00:00:00
abstract:UNLABELLED:Human noroviruses (HuNoVs) cause significant morbidity and mortality worldwide. However, despite substantial efforts, a small-animal model for HuNoV has not been described to date. Since "humanized" mice have been successfully used to study human-tropic pathogens in the past, we challenged BALB/c mice defici...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00450-13
更新日期:2013-07-16 00:00:00
abstract::Increasing evidence has indicated that single nucleotide polymorphisms (SNPs) are related to the susceptibility of sepsis and might provide potential evidence for the mechanisms of sepsis. Our recent preliminary study showed that the ADAM10 genetic polymorphism was clinically associated with the development of sepsis,...
journal_title:mBio
pub_type: 杂志文章,多中心研究
doi:10.1128/mBio.01663-19
更新日期:2019-08-06 00:00:00
abstract:UNLABELLED:Recent studies strongly suggest that the gene expression sustaining both normal and pathogenic bacterial growth is governed by the structural dynamics of the chromosome. However, the mechanistic device coordinating the chromosomal configuration with selective expression of the adaptive traits remains largely...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00353-15
更新日期:2015-04-28 00:00:00
abstract::Protein kinase R (PKR) plays a major role in activating host immunity during infection by sensing double-stranded RNA (dsRNA) produced by viruses. Once activated by dsRNA, PKR phosphorylates the translation factor eukaryotic initiation factor 2α (eIF2α), halting cellular translation. Many viruses have methods of inhib...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00668-19
更新日期:2019-04-23 00:00:00
abstract:UNLABELLED:The group of proteins known as serine protease autotransporters of Enterobacteriaceae (SPATE) is a growing family of serine proteases secreted to the external milieu by the type V secretion system. Pet toxin and some other SPATE belong to the class 1 cytotoxic SPATE, which have comparable protease strength o...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00838-13
更新日期:2013-12-10 00:00:00
abstract::The biosynthesis of antioxidant pigments, namely, betalains, was believed to be restricted to Caryophyllales plants. This paper changes this paradigm, and enzyme mining from bacterial hosts promoted the discovery of bacterial cultures producing betalains. The spectrum of possible sources of betalain pigments in nature...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00345-19
更新日期:2019-03-19 00:00:00
abstract:UNLABELLED:Glycoside hydrolases (GHs) are key enzymes in the depolymerization of plant-derived cellulose, a process central to the global carbon cycle and the conversion of plant biomass to fuels and chemicals. A limited number of GH families hydrolyze crystalline cellulose, often by a processive mechanism along the ce...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01106-16
更新日期:2016-08-23 00:00:00
abstract::Although the TEM-1 β-lactamase (BlaTEM-1) hydrolyzes penicillins and narrow-spectrum cephalosporins, organisms expressing this enzyme are typically susceptible to β-lactam/β-lactamase inhibitor combinations such as piperacillin-tazobactam (TZP). However, our previous work led to the discovery of 28 clinical isolates o...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00583-18
更新日期:2018-04-24 00:00:00
abstract::Chlamydiales species are obligate intracellular bacteria lacking a classical peptidoglycan sacculus but relying on peptidoglycan synthesis for cytokinesis. While septal peptidoglycan biosynthesis seems to be regulated by MreB actin and its membrane anchor RodZ rather than FtsZ tubulin in Chlamydiales, the mechanism of...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01128-19
更新日期:2019-07-16 00:00:00
abstract::Enterococci are ancient commensal bacteria that recently emerged as leading causes of antibiotic-resistant, hospital-acquired infection. Vancomycin-resistant enterococci (VRE) epitomize why drug-resistant enterococcal infections are a problem: VRE readily colonize the antibiotic-perturbed gastrointestinal (GI) tract w...
journal_title:mBio
pub_type: 评论,杂志文章
doi:10.1128/mBio.00066-17
更新日期:2017-02-21 00:00:00
abstract::A simple method for filtering water to reduce the incidence of cholera was tested in a field trial in Matlab, Bangladesh, and proved effective. A follow-up study was conducted 5 years later to determine whether the filtration method continued to be employed by villagers and its impact on the incidence of cholera. A to...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00034-10
更新日期:2010-05-18 00:00:00
abstract:UNLABELLED:Helicobacter pylori chronically infects the gastric mucosa in more than half of the human population; in a subset of this population, its presence is associated with development of severe disease, such as gastric cancer. Genomic analysis of several strains has revealed an extensive H. pylori pan-genome, like...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00239-11
更新日期:2011-11-15 00:00:00
abstract:UNLABELLED:Cryptococcus neoformans is a human fungal pathogen and a major cause of fungal meningitis in immunocompromised individuals. Treatment options for cryptococcosis are limited. Of the two major antifungal drug classes, azoles are active against C. neoformans but exert a fungistatic effect, necessitating long tr...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00478-16
更新日期:2016-05-10 00:00:00
abstract::Acute flaccid myelitis (AFM) is a rare but serious illness of the nervous system, specifically affecting the gray matter of the spinal cord, motor-controlling regions of the brain, and cranial nerves. Most cases of AFM are pathogen associated, typically with poliovirus and enterovirus infections, and occur in children...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02370-19
更新日期:2019-10-22 00:00:00
abstract::Mycobacterium bovis BCG is the only available vaccine for protection against tuberculosis (TB). While BCG protects children from severe disease, it has little impact on pulmonary disease in adults. A recombinant BCG vaccine BCG ΔureC::hly (strain VPM1002) is in advanced clinical trials and shows promise for improved v...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01370-16
更新日期:2016-08-30 00:00:00
abstract:UNLABELLED:Epstein-Barr virus (EBV), along with other members of the herpesvirus family, requires a set of viral glycoproteins to mediate host cell attachment and entry. Viral glycoprotein B (gB), a highly conserved glycoprotein within the herpesvirus family, is thought to be the viral fusogen based on structural compa...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00571-12
更新日期:2013-01-22 00:00:00
abstract::The mammalian gut contains a complex assembly of commensal microbes termed microbiota. Although much has been learned about the role of these microbes in health, the mechanisms underlying these functions are ill defined. We have recently shown that the mammalian gut contains thousands of small molecules, most of which...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01183-14
更新日期:2014-07-29 00:00:00
abstract::Symbiotic microorganisms can have a profound impact on the host physiology and behavior, and novel relationships between symbionts and their hosts are continually discovered. A colony of social ants consists of various castes that exhibit distinct lifestyles and is, thus, a unique model for investigating how symbionts...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00408-20
更新日期:2020-04-21 00:00:00
abstract:UNLABELLED:Conjugate vaccines are known to be one of the most effective and safest types of vaccines against bacterial pathogens. Previously, vaccine biosynthesis has been performed by using N-linked glycosylation systems. However, the structural specificity of these systems for sugar substrates has hindered their appl...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00443-16
更新日期:2016-04-26 00:00:00
abstract::Nicotine, a toxic and addictive alkaloid from tobacco, is an environmental pollutant in areas near cigarette production facilities. Over the last decade, our group has studied, in depth, the pyrrolidine pathway of nicotine degradation in Pseudomonas putida S16. However, little is known regarding whole mechanism(s) reg...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00602-19
更新日期:2019-06-04 00:00:00
abstract::Forested ecosystems throughout the world are experiencing increases in the incidence and magnitude of insect-induced tree mortality with large ecologic ramifications. Interestingly, correlations between water quality and the extent of tree mortality in Colorado montane ecosystems suggest compensatory effects from adja...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01305-17
更新日期:2017-12-05 00:00:00
abstract::Cellular sensing of bacterial RNA is increasingly recognized as a determinant of host-pathogen interactions. The intracellular pathogen Listeria monocytogenes induces high levels of type I interferons (alpha/beta interferons [IFN-α/β]) to create a growth-permissive microenvironment during infection. We previously demo...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01223-19
更新日期:2019-10-08 00:00:00
abstract::The rapid increase of carbapenem resistance in Gram-negative bacteria has resurrected the importance of the polymyxin antibiotics. The recent discovery of plasmid-mediated polymyxin resistance (mcr-1) in carbapenem-resistant Enterobacteriaceae serves as an important indicator that the golden era of antibiotics is unde...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00540-17
更新日期:2017-07-25 00:00:00