A SpoIID Homolog Cleaves Glycan Strands at the Chlamydial Division Septum.


:Chlamydiales species are obligate intracellular bacteria lacking a classical peptidoglycan sacculus but relying on peptidoglycan synthesis for cytokinesis. While septal peptidoglycan biosynthesis seems to be regulated by MreB actin and its membrane anchor RodZ rather than FtsZ tubulin in Chlamydiales, the mechanism of peptidoglycan remodeling is poorly understood. An amidase conserved in Chlamydiales is able to cleave peptide stems in peptidoglycan, but it is not clear how peptidoglycan glycan strands are cleaved since no classical lytic transglycosylase is encoded in chlamydial genomes. However, a protein containing a SpoIID domain, known to possess transglycosylase activity in Bacillus subtilis, is conserved in Chlamydiales We show here that the SpoIID homologue of the Chlamydia-related pathogen Waddlia chondrophila is a septal peptidoglycan-binding protein. Moreover, we demonstrate that SpoIID acts as a lytic transglycosylase on peptidoglycan and as a muramidase on denuded glycan strands in vitro As SpoIID-like proteins are widespread in nonsporulating bacteria, SpoIID might commonly be a septal peptidoglycan remodeling protein in bacteria, including obligate intracellular pathogens, and thus might represent a promising drug target.IMPORTANCEChlamydiales species are obligate intracellular bacteria and important human pathogens that have a minimal division machinery lacking the proteins that are essential for bacterial division in other species, such as FtsZ. Chlamydial division requires synthesis of peptidoglycan, which forms a ring at the division septum and is rapidly turned over. However, little is known of peptidoglycan degradation, because many peptidoglycan-degrading enzymes are not encoded by chlamydial genomes. Here we show that an homologue of SpoIID, a peptidoglycan-degrading enzyme involved in sporulation of bacteria such as Bacillus subtilis, is expressed in Chlamydiales, localizes at the division septum, and degrades peptidoglycan in vitro, indicating that SpoIID is not only involved in sporulation but also likely implicated in division of some bacteria.






Jacquier N,Yadav AK,Pillonel T,Viollier PH,Cava F,Greub G




Has Abstract


2019-07-16 00:00:00












  • Methane Seep Carbonates Host Distinct, Diverse, and Dynamic Microbial Assemblages.

    abstract:UNLABELLED:Marine methane seeps are globally distributed geologic features in which reduced fluids, including methane, are advected upward from the subsurface. As a result of alkalinity generation during sulfate-coupled methane oxidation, authigenic carbonates form slabs, nodules, and extensive pavements. These carbona...


    pub_type: 杂志文章


    authors: Case DH,Pasulka AL,Marlow JJ,Grupe BM,Levin LA,Orphan VJ

    更新日期:2015-12-22 00:00:00

  • The Escherichia coli protein YfeX functions as a porphyrinogen oxidase, not a heme dechelatase.

    abstract:UNLABELLED:The protein YfeX from Escherichia coli has been proposed to be essential for the process of iron removal from heme by carrying out a dechelation of heme without cleavage of the porphyrin macrocycle. Since this proposed reaction is unique and would represent the first instance of the biological dechelation of...


    pub_type: 杂志文章


    authors: Dailey HA,Septer AN,Daugherty L,Thames D,Gerdes S,Stabb EV,Dunn AK,Dailey TA,Phillips JD

    更新日期:2011-11-08 00:00:00

  • In Vivo Biotinylation of the Toxoplasma Parasitophorous Vacuole Reveals Novel Dense Granule Proteins Important for Parasite Growth and Pathogenesis.

    abstract:UNLABELLED:Toxoplasma gondii is an obligate intracellular parasite that invades host cells and replicates within a unique parasitophorous vacuole. To maintain this intracellular niche, the parasite secretes an array of dense granule proteins (GRAs) into the nascent parasitophorous vacuole. These GRAs are believed to pl...


    pub_type: 杂志文章


    authors: Nadipuram SM,Kim EW,Vashisht AA,Lin AH,Bell HN,Coppens I,Wohlschlegel JA,Bradley PJ

    更新日期:2016-08-02 00:00:00

  • Hepatitis C Virus Induces the Ubiquitin-Editing Enzyme A20 via Depletion of the Transcription Factor Upstream Stimulatory Factor 1 To Support Its Replication.

    abstract::Tumor necrosis factor alpha-induced protein 3 (TNFAIP3), also known as A20, is a ubiquitin-editing enzyme capable of ubiquitination or deubiquitination of its target proteins. In this study, we show that hepatitis C virus (HCV) infection could induce the expression of A20 via the activation of the A20 promoter. The in...


    pub_type: 杂志文章


    authors: Lee J,Chan ST,Kim JY,Ou JJ

    更新日期:2019-07-23 00:00:00

  • Host Cell S Phase Restricts Legionella pneumophila Intracellular Replication by Destabilizing the Membrane-Bound Replication Compartment.

    abstract::Legionella pneumophila grows within cells ranging from environmental amoebae to human macrophages. In spite of this conserved strategy of pathogenesis, identification of host factors that restrict L. pneumophila intracellular replication has not been extended outside components of the mammalian innate immune response....


    pub_type: 杂志文章


    authors: de Jesús-Díaz DA,Murphy C,Sol A,Dorer M,Isberg RR

    更新日期:2017-08-22 00:00:00

  • Biosynthesis of the unique wall teichoic acid of Staphylococcus aureus lineage ST395.

    abstract::ABSTRACT The major clonal lineages of the human pathogen Staphylococcus aureus produce cell wall-anchored anionic poly-ribitol-phosphate (RboP) wall teichoic acids (WTA) substituted with d-Alanine and N-acetyl-d-glucosamine. The phylogenetically isolated S. aureus ST395 lineage has recently been found to produce a uni...


    pub_type: 杂志文章


    authors: Winstel V,Sanchez-Carballo P,Holst O,Xia G,Peschel A

    更新日期:2014-04-08 00:00:00

  • Low Polymerase Activity Attributed to PA Drives the Acquisition of the PB2 E627K Mutation of H7N9 Avian Influenza Virus in Mammals.

    abstract::Avian influenza viruses (AIVs) must acquire mammalian-adaptive mutations before they can efficiently replicate in and transmit among humans. The PB2 E627K mutation is known to play a prominent role in the mammalian adaptation of AIVs. The H7N9 AIVs that emerged in 2013 in China easily acquired the PB2 E627K mutation u...


    pub_type: 杂志文章


    authors: Liang L,Jiang L,Li J,Zhao Q,Wang J,He X,Huang S,Wang Q,Zhao Y,Wang G,Sun N,Deng G,Shi J,Tian G,Zeng X,Jiang Y,Liu L,Liu J,Chen P,Bu Z,Kawaoka Y,Chen H,Li C

    更新日期:2019-06-18 00:00:00

  • The Toxoplasma Centrocone Houses Cell Cycle Regulatory Factors.

    abstract::Our knowledge of cell cycle regulatory mechanisms in apicomplexan parasites is very limited. In this study, we describe a novel Toxoplasma gondii factor that has a vital role in chromosome replication and the regulation of cytoplasmic and nuclear mitotic structures, and we named this factor ECR1 for essential for chro...


    pub_type: 杂志文章


    authors: Naumov A,Kratzer S,Ting LM,Kim K,Suvorova ES,White MW

    更新日期:2017-08-22 00:00:00

  • Population genetics of Vibrio cholerae from Nepal in 2010: evidence on the origin of the Haitian outbreak.

    abstract::Cholera continues to be an important cause of human infections, and outbreaks are often observed after natural disasters, such as the one following the 2010 earthquake in Haiti. Once the cholera outbreak was confirmed, rumors spread that the disease was brought to Haiti by a battalion of Nepalese soldiers serving as U...


    pub_type: 杂志文章


    authors: Hendriksen RS,Price LB,Schupp JM,Gillece JD,Kaas RS,Engelthaler DM,Bortolaia V,Pearson T,Waters AE,Upadhyay BP,Shrestha SD,Adhikari S,Shakya G,Keim PS,Aarestrup FM

    更新日期:2011-09-01 00:00:00

  • Septins arrange F-actin-containing fibers on the Chlamydia trachomatis inclusion and are required for normal release of the inclusion by extrusion.

    abstract::Chlamydia trachomatis is an obligate intracellular human pathogen that grows inside a membranous, cytosolic vacuole termed an inclusion. Septins are a group of 13 GTP-binding proteins that assemble into oligomeric complexes and that can form higher-order filaments. We report here that the septins SEPT2, -9, -11, and p...


    pub_type: 杂志文章


    authors: Volceanov L,Herbst K,Biniossek M,Schilling O,Haller D,Nölke T,Subbarayal P,Rudel T,Zieger B,Häcker G

    更新日期:2014-10-07 00:00:00

  • Defining the DNA Binding Site Recognized by the Fission Yeast Zn2Cys6 Transcription Factor Pho7 and Its Role in Phosphate Homeostasis.

    abstract::Fission yeast phosphate homeostasis entails transcriptional induction of genes encoding phosphate-mobilizing proteins under conditions of phosphate starvation. Transcription factor Pho7, a member of the Zn2Cys6 family of fungal transcription regulators, is the central player in the starvation response. The DNA binding...


    pub_type: 杂志文章


    authors: Schwer B,Sanchez AM,Garg A,Chatterjee D,Shuman S

    更新日期:2017-08-15 00:00:00

  • Antibodies Elicited by an NS1-Based Vaccine Protect Mice against Zika Virus.

    abstract::Zika virus is a mosquito-borne flavivirus which can cause severe disease in humans, including microcephaly and other congenital malformations in newborns and Guillain-Barré syndrome in adults. There are currently no approved prophylactics or therapeutics for Zika virus; the development of a safe and effective vaccine ...


    pub_type: 杂志文章


    authors: Bailey MJ,Broecker F,Duehr J,Arumemi F,Krammer F,Palese P,Tan GS

    更新日期:2019-04-02 00:00:00

  • The role of clonal interference in the evolutionary dynamics of plasmid-host adaptation.

    abstract:UNLABELLED:Promiscuous plasmids replicate in a wide range of bacteria and therefore play a key role in the dissemination of various host-beneficial traits, including antibiotic resistance. Despite the medical relevance, little is known about the evolutionary dynamics through which drug resistance plasmids adapt to new ...


    pub_type: 杂志文章


    authors: Hughes JM,Lohman BK,Deckert GE,Nichols EP,Settles M,Abdo Z,Top EM

    更新日期:2012-07-03 00:00:00

  • Type III Interferon-Mediated Signaling Is Critical for Controlling Live Attenuated Yellow Fever Virus Infection In Vivo.

    abstract::Yellow fever virus (YFV) is an arthropod-borne flavivirus, infecting ~200,000 people worldwide annually and causing about 30,000 deaths. The live attenuated vaccine strain, YFV-17D, has significantly contributed in controlling the global burden of yellow fever worldwide. However, the viral and host contributions to YF...


    pub_type: 杂志文章


    authors: Douam F,Soto Albrecht YE,Hrebikova G,Sadimin E,Davidson C,Kotenko SV,Ploss A

    更新日期:2017-08-15 00:00:00

  • Identification and Characterization of a Phase-Variable Element That Regulates the Autotransporter UpaE in Uropathogenic Escherichia coli.

    abstract::Uropathogenic Escherichia coli (UPEC) is the most common etiologic agent of uncomplicated urinary tract infection (UTI). An important mechanism of gene regulation in UPEC is phase variation that involves inversion of a promoter-containing DNA element via enzymatic activity of tyrosine recombinases, resulting in biphas...


    pub_type: 杂志文章


    authors: Battaglioli EJ,Goh KGK,Atruktsang TS,Schwartz K,Schembri MA,Welch RA

    更新日期:2018-08-07 00:00:00

  • The Antituberculosis Drug Ethambutol Selectively Blocks Apical Growth in CMN Group Bacteria.

    abstract::Members of the genus Mycobacterium are the most prevalent cause of infectious diseases. Mycobacteria have a complex cell envelope containing a peptidoglycan layer and an additional arabinogalactan polymer to which a mycolic acid bilayer is linked; this complex, multilayered cell wall composition (mAGP) is conserved am...


    pub_type: 杂志文章


    authors: Schubert K,Sieger B,Meyer F,Giacomelli G,Böhm K,Rieblinger A,Lindenthal L,Sachs N,Wanner G,Bramkamp M

    更新日期:2017-02-07 00:00:00

  • Genomic Analysis of Hospital Plumbing Reveals Diverse Reservoir of Bacterial Plasmids Conferring Carbapenem Resistance.

    abstract::The hospital environment is a potential reservoir of bacteria with plasmids conferring carbapenem resistance. Our Hospital Epidemiology Service routinely performs extensive sampling of high-touch surfaces, sinks, and other locations in the hospital. Over a 2-year period, additional sampling was conducted at a broader ...


    pub_type: 杂志文章


    authors: Weingarten RA,Johnson RC,Conlan S,Ramsburg AM,Dekker JP,Lau AF,Khil P,Odom RT,Deming C,Park M,Thomas PJ,NISC Comparative Sequencing Program.,Henderson DK,Palmore TN,Segre JA,Frank KM

    更新日期:2018-02-06 00:00:00

  • Membrane Distribution of the Pseudomonas Quinolone Signal Modulates Outer Membrane Vesicle Production in Pseudomonas aeruginosa.

    abstract::The Pseudomonas quinolone signal (PQS) is an important quorum-sensing molecule in Pseudomonas aeruginosa that also mediates its own packaging and transport by stimulating outer membrane vesicle (OMV) formation. Because OMVs have been implicated in many virulence-associated behaviors, it is critical that we understand ...


    pub_type: 杂志文章


    authors: Florez C,Raab JE,Cooke AC,Schertzer JW

    更新日期:2017-08-08 00:00:00

  • A Shift in Central Metabolism Accompanies Virulence Activation in Pseudomonas aeruginosa.

    abstract::The availability of energy has significant impact on cell physiology. However, the role of cellular metabolism in bacterial pathogenesis is not understood. We investigated the dynamics of central metabolism during virulence induction by surface sensing and quorum sensing in early-stage biofilms of the multidrug-resist...


    pub_type: 杂志文章


    authors: Perinbam K,Chacko JV,Kannan A,Digman MA,Siryaporn A

    更新日期:2020-03-10 00:00:00

  • Chromatin Modulation of Herpesvirus Lytic Gene Expression: Managing Nucleosome Density and Heterochromatic Histone Modifications.

    abstract::Like their cellular hosts, herpesviruses are subject to the regulatory impacts of chromatin assembled on their genomes. Upon infection, these viruses are assembled into domains of chromatin with heterochromatic signatures that suppress viral gene expression or euchromatic characteristics that promote gene expression. ...


    pub_type: 杂志文章


    authors: Kristie TM

    更新日期:2016-02-16 00:00:00

  • Ecosystem Resilience and Limitations Revealed by Soil Bacterial Community Dynamics in a Bark Beetle-Impacted Forest.

    abstract::Forested ecosystems throughout the world are experiencing increases in the incidence and magnitude of insect-induced tree mortality with large ecologic ramifications. Interestingly, correlations between water quality and the extent of tree mortality in Colorado montane ecosystems suggest compensatory effects from adja...


    pub_type: 杂志文章


    authors: Mikkelson KM,Brouillard BM,Bokman CM,Sharp JO

    更新日期:2017-12-05 00:00:00

  • Polymyxin Combinations Combat Escherichia coli Harboring mcr-1 and blaNDM-5: Preparation for a Postantibiotic Era.

    abstract::The rapid increase of carbapenem resistance in Gram-negative bacteria has resurrected the importance of the polymyxin antibiotics. The recent discovery of plasmid-mediated polymyxin resistance (mcr-1) in carbapenem-resistant Enterobacteriaceae serves as an important indicator that the golden era of antibiotics is unde...


    pub_type: 杂志文章


    authors: Bulman ZP,Chen L,Walsh TJ,Satlin MJ,Qian Y,Bulitta JB,Peloquin CA,Holden PN,Nation RL,Li J,Kreiswirth BN,Tsuji BT

    更新日期:2017-07-25 00:00:00

  • Hybrid Vibrio cholerae El Tor lacking SXT identified as the cause of a cholera outbreak in the Philippines.

    abstract:UNLABELLED:Cholera continues to be a global threat, with high rates of morbidity and mortality. In 2011, a cholera outbreak occurred in Palawan, Philippines, affecting more than 500 people, and 20 individuals died. Vibrio cholerae O1 was confirmed as the etiological agent. Source attribution is critical in cholera outb...


    pub_type: 杂志文章


    authors: Klinzing DC,Choi SY,Hasan NA,Matias RR,Tayag E,Geronimo J,Skowronski E,Rashed SM,Kawashima K,Rosenzweig CN,Gibbons HS,Torres BC,Liles V,Alfon AC,Juan ML,Natividad FF,Cebula TA,Colwell RR

    更新日期:2015-04-21 00:00:00

  • Structure of the Receptor Binding Domain of EnvP(b)1, an Endogenous Retroviral Envelope Protein Expressed in Human Tissues.

    abstract::EnvP(b)1 is an endogenous retroviral envelope gene found in human and other primate genomes. We report EnvP(b)1 sequences in primate genomes consistent with an integration event between 40 and 71 million years ago. Using a highly specific polyclonal antiserum raised against the putative receptor binding domain (RBD) o...


    pub_type: 杂志文章


    authors: McCarthy KR,Timpona JL,Jenni S,Bloyet LM,Brusic V,Johnson WE,Whelan SPJ,Robinson-McCarthy LR

    更新日期:2020-11-17 00:00:00

  • Clathrin- and Arp2/3-independent endocytosis in the fungal pathogen Candida albicans.

    abstract:UNLABELLED:Clathrin-mediated endocytosis (CME) is conserved among eukaryotes and has been extensively analyzed at a molecular level. Here, we present an analysis of CME in the human fungal pathogen Candida albicans that shows the same modular structure as those in other fungi and mammalian cells. Intriguingly, C. albic...


    pub_type: 杂志文章


    authors: Epp E,Nazarova E,Regan H,Douglas LM,Konopka JB,Vogel J,Whiteway M

    更新日期:2013-08-27 00:00:00

  • Two KTR Mannosyltransferases Are Responsible for the Biosynthesis of Cell Wall Mannans and Control Polarized Growth in Aspergillus fumigatus.

    abstract::Fungal cell wall mannans are complex carbohydrate polysaccharides with different structures in yeasts and molds. In contrast to yeasts, their biosynthetic pathway has been poorly investigated in filamentous fungi. In Aspergillus fumigatus, the major mannan structure is a galactomannan that is cross-linked to the β-1,3...


    pub_type: 杂志文章


    authors: Henry C,Li J,Danion F,Alcazar-Fuoli L,Mellado E,Beau R,Jouvion G,Latgé JP,Fontaine T

    更新日期:2019-02-12 00:00:00

  • The Novel J-Domain Protein Mrj1 Is Required for Mitochondrial Respiration and Virulence in Cryptococcus neoformans.

    abstract::The opportunistic fungal pathogen Cryptococcus neoformans must adapt to the mammalian environment to establish an infection. Proteins facilitating adaptation to novel environments, such as chaperones, may be required for virulence. In this study, we identified a novel mitochondrial co-chaperone, Mrj1 (mitochondrial re...


    pub_type: 杂志文章


    authors: Horianopoulos LC,Hu G,Caza M,Schmitt K,Overby P,Johnson JD,Valerius O,Braus GH,Kronstad JW

    更新日期:2020-06-09 00:00:00

  • Iron Corrosion via Direct Metal-Microbe Electron Transfer.

    abstract::The concept that anaerobic microorganisms can directly accept electrons from Fe(0) has been controversial because direct metal-microbe electron transfer has previously only been indirectly inferred. Fe(0) oxidation was studied with Geobacter sulfurreducens strain ACL, an autotrophic strain that was previously shown to...


    pub_type: 杂志文章


    authors: Tang HY,Holmes DE,Ueki T,Palacios PA,Lovley DR

    更新日期:2019-05-14 00:00:00

  • In Situ Molecular Architecture of the Helicobacter pylori Cag Type IV Secretion System.

    abstract::Helicobacter pylori colonizes about half of humans worldwide, and its presence in the gastric mucosa is associated with an increased risk of gastric adenocarcinoma, gastric lymphoma, and peptic ulcer disease. H. pylori strains carrying the cag pathogenicity island (cagPAI) are associated with increased risk of disease...


    pub_type: 杂志文章


    authors: Hu B,Khara P,Song L,Lin AS,Frick-Cheng AE,Harvey ML,Cover TL,Christie PJ

    更新日期:2019-05-14 00:00:00

  • Vesicular trafficking of incoming human papillomavirus 16 to the Golgi apparatus and endoplasmic reticulum requires γ-secretase activity.

    abstract:UNLABELLED:The route taken by papillomaviruses from the cell surface to the nucleus during infection is incompletely understood. Here, we developed a novel human papillomavirus 16 (HPV16) pseudovirus in which the carboxy terminus of the minor capsid protein L2 is exposed on the exterior of the intact capsid prior to ce...


    pub_type: 杂志文章


    authors: Zhang W,Kazakov T,Popa A,DiMaio D

    更新日期:2014-09-16 00:00:00