Abstract:
:Bordetella pertussis controls the expression of its virulence regulon through the two-component system BvgAS. BvgS is a prototype for a family of multidomain sensor kinases. In BvgS, helical linkers connect periplasmic Venus flytrap (VFT) perception domains to a cytoplasmic Per-Arnt-Sim (PAS) domain and the PAS domain to the dimerization/histidine phosphotransfer (DHp) domain of the kinase. The two linkers can adopt coiled-coil structures but cannot do so simultaneously. The first linker forms a coiled coil in the kinase mode and the second in the phosphatase mode, with the other linker in both cases showing an increase in dynamic behavior. The intervening PAS domain changes its quaternary structure between the two modes. In BvgS homologues without a PAS domain, a helical "X" linker directly connects the VFT and DHp domains. Here, we used BvgS as a platform to characterize regulation in members of the PAS-less subfamily. BvgS chimeras of homologues with natural X linkers displayed various regulation phenotypes. We identified two distinct coiled-coil registers in the N- and C-terminal portions of the X linkers. Stable coil formation in the C-terminal moiety determines the phosphatase mode, similarly to BvgS; in contrast, coil formation in the N-terminal moiety along the other register leads to the kinase mode. Thus, antagonism between two registers in the VFT-DHp linker forms the basis for activity regulation in the absence of the PAS domain. The N and C moieties of the X linker play roles similar to those played by the two independent linkers in sensor kinases with a PAS domain, providing a unified mechanism of regulation for the entire family.IMPORTANCE The whooping cough agent Bordetella pertussis uses the BvgAS sensory transduction two-component system to regulate production of its virulence factors. BvgS serves as a model for a large family of multidomain bacterial sensor kinases. B. pertussis is virulent when BvgS functions as a kinase and avirulent when it switches to phosphatase activity in response to modulating signals. Understanding the molecular regulation of those proteins might lead to new antibacterial strategies. Here, we show that the linker regions between the perception and the enzymatic domains shift between distinct states of conformation in an alternating manner in response to signals and that their antagonistic changes control sensor kinase activity. These linker regions and mechanistic principles appear to be conserved among BvgS homologues, irrespective of the presence or absence of an intervening domain between the perception and the enzymatic domains. This work has thus uncovered general molecular mechanisms that regulate activity of sensor kinases in the BvgS family.
journal_name
mBiojournal_title
mBioauthors
Lesne E,Dupré E,Lensink MF,Locht C,Antoine R,Jacob-Dubuisson Fdoi
10.1128/mBio.02052-17subject
Has Abstractpub_date
2018-02-27 00:00:00issue
1issn
2150-7511pii
mBio.02052-17journal_volume
9pub_type
杂志文章相关文献
mBio文献大全abstract::Host-associated microbial communities are shaped by extrinsic and intrinsic factors to the holobiont organism. Environmental factors and microbe-microbe interactions act simultaneously on the microbial community structure, making the microbiome dynamics challenging to predict. The coral microbiome is essential to the ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02691-19
更新日期:2020-03-03 00:00:00
abstract::ABSTRACT The major clonal lineages of the human pathogen Staphylococcus aureus produce cell wall-anchored anionic poly-ribitol-phosphate (RboP) wall teichoic acids (WTA) substituted with d-Alanine and N-acetyl-d-glucosamine. The phylogenetically isolated S. aureus ST395 lineage has recently been found to produce a uni...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00869-14
更新日期:2014-04-08 00:00:00
abstract::Peroxisomes are found in essentially all eukaryotic cells and have been described as important hubs in innate sensing and the induction of type III interferons upon viral infection. Nevertheless, it remains poorly investigated how viral pathogens modulate biogenesis or function of peroxisomes to evade innate sensing a...
journal_title:mBio
pub_type: 评论,杂志文章
doi:10.1128/mBio.00967-20
更新日期:2020-05-26 00:00:00
abstract::The alphaproteobacterial general stress response is governed by a conserved partner-switching mechanism that is triggered by phosphorylation of the response regulator PhyR. In the model organism Caulobacter crescentus, PhyR was proposed to be phosphorylated by the histidine kinase PhyK, but biochemical evidence in sup...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00809-18
更新日期:2018-05-22 00:00:00
abstract::To address the question of how microbial diversity and function in the oral cavities of children relates to caries diagnosis, we surveyed the supragingival plaque biofilm microbiome in 44 juvenile twin pairs. Using shotgun sequencing, we constructed a genome encyclopedia describing the core supragingival plaque microb...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01631-18
更新日期:2018-11-27 00:00:00
abstract::Regulated organization of the chromosome is essential for faithful propagation of genetic information. In the model bacterium Caulobacter crescentus, the replication terminus of the chromosome is spatially arranged in close proximity to the cytokinetic Z-ring during the cell cycle. Although the Z-ring-associated prote...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02196-20
更新日期:2021-01-26 00:00:00
abstract::The concept that anaerobic microorganisms can directly accept electrons from Fe(0) has been controversial because direct metal-microbe electron transfer has previously only been indirectly inferred. Fe(0) oxidation was studied with Geobacter sulfurreducens strain ACL, an autotrophic strain that was previously shown to...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00303-19
更新日期:2019-05-14 00:00:00
abstract::Bacterial viruses, or bacteriophages, are highly abundant in the biosphere and have a major impact on microbial populations. Many examples of phage interactions with their hosts, including establishment of dormant lysogenic and active lytic states, have been characterized at the level of the individual cell. However, ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01884-19
更新日期:2019-09-10 00:00:00
abstract::Host cytokine responses to Brucella abortus infection are elicited predominantly by the deployment of a type IV secretion system (T4SS). However, the mechanism by which the T4SS elicits inflammation remains unknown. Here we show that translocation of the T4SS substrate VceC into host cells induces proinflammatory resp...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00418-12
更新日期:2013-02-19 00:00:00
abstract::The opportunistic pathogen Pseudomonas aeruginosa is a leading cause of airway infection in cystic fibrosis (CF) patients. P. aeruginosa employs several hierarchically arranged and interconnected quorum sensing (QS) regulatory circuits to produce a battery of virulence factors such as elastase, phenazines, and rhamnol...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00532-20
更新日期:2020-04-07 00:00:00
abstract:UNLABELLED:Carbonate chimneys at the Lost City hydrothermal field are coated in biofilms dominated by a single phylotype of archaea known as Lost City Methanosarcinales. In this study, we have detected surprising physiological complexity in single-species biofilms, which is typically indicative of multispecies biofilm ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00127-11
更新日期:2011-09-01 00:00:00
abstract::Nineteen Thermococcus kodakarensis strains have been constructed, each of which synthesizes a different His(6)-tagged protein known or predicted to be a component of the archaeal DNA replication machinery. Using the His(6)-tagged proteins, stable complexes assembled in vivo have been isolated directly from clarified c...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00221-10
更新日期:2010-10-26 00:00:00
abstract::Influenza A viruses cause an annual contagious respiratory disease in humans and are responsible for periodic high-mortality human pandemics. Pandemic influenza A viruses usually result from the reassortment of gene segments between human and avian influenza viruses. These avian influenza virus gene segments need to a...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01222-18
更新日期:2018-07-03 00:00:00
abstract::Helicobacter pylori colonizes about half of humans worldwide, and its presence in the gastric mucosa is associated with an increased risk of gastric adenocarcinoma, gastric lymphoma, and peptic ulcer disease. H. pylori strains carrying the cag pathogenicity island (cagPAI) are associated with increased risk of disease...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00849-19
更新日期:2019-05-14 00:00:00
abstract:UNLABELLED:The gut microbiota enhances the host's metabolic capacity for processing nutrients and drugs and modulate the activities of multiple pathways in a variety of organ systems. We have probed the systemic metabolic adaptation to gut colonization for 20 days following exposure of axenic mice (n = 35) to a typical...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00271-10
更新日期:2011-03-01 00:00:00
abstract:UNLABELLED:Singlet oxygen ((1)O(2)) is a reactive oxygen species generated by energy transfer from one or more excited donors to molecular oxygen. Many biomolecules are prone to oxidation by (1)O(2), and cells have evolved systems to protect themselves from damage caused by this compound. One way that the photosyntheti...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00541-12
更新日期:2013-01-08 00:00:00
abstract::Resistance-nodulation-division (RND) superfamily efflux systems have been widely studied for their role in antibiotic resistance, but their native biological functions remain poorly understood. We previously showed that loss of RND-mediated efflux in Vibrio cholerae resulted in activation of the Cpx two-component regu...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00126-17
更新日期:2017-05-16 00:00:00
abstract::RNA-binding proteins (RBPs) play a major role during control of mRNA localization, stability, and translation and are central to most cellular processes. In the fission yeast Schizosaccharomyces pombe, the multiple K homology (KH) domain RBP Rnc1 downregulates the activity of the cell integrity pathway (CIP) via stabi...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02815-19
更新日期:2020-01-07 00:00:00
abstract::1-Butanol is a large-volume, intermediate chemical with favorable physical and chemical properties for blending with or directly substituting for gasoline. The per-volume value of butanol, as a chemical, is sufficient for investing into the recommercialization of the classical acetone-butanol-ethanol (ABE) (E. M. Gree...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00518-12
更新日期:2012-12-11 00:00:00
abstract::Malaria parasites have a complex life cycle with multiple developmental stages in mosquito and vertebrate hosts, and different developmental stages express unique sets of genes. Unexpectedly, many transcription factors (TFs) commonly found in eukaryotic organisms are absent in malaria parasites; instead, a family of g...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01986-17
更新日期:2017-12-12 00:00:00
abstract::Legionella pneumophila grows within cells ranging from environmental amoebae to human macrophages. In spite of this conserved strategy of pathogenesis, identification of host factors that restrict L. pneumophila intracellular replication has not been extended outside components of the mammalian innate immune response....
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02345-16
更新日期:2017-08-22 00:00:00
abstract::The chemistry underpinning microbial interactions provides an integrative framework for linking the activities of individual microbes, microbial communities, plants, and their environments. Currently, we know very little about the functions of genes and metabolites within these communities because genome annotations a...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01175-18
更新日期:2018-07-17 00:00:00
abstract::Interindividual variation in the composition of the human gut microbiome was examined in relation to demographic and anthropometric traits, and to changes in dietary saturated fat intake and protein source. One hundred nine healthy men and women aged 21 to 65, with BMIs of 18 to 36, were randomized, after a two-week b...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01604-18
更新日期:2018-12-11 00:00:00
abstract::Dephospho-coenzyme A (dephospho-CoA) kinase (DPCK) catalyzes the ATP-dependent phosphorylation of dephospho-CoA, the final step in coenzyme A (CoA) biosynthesis. DPCK has been identified and characterized in bacteria and eukaryotes but not in archaea. The hyperthermophilic archaeon Thermococcus kodakarensis encodes tw...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01146-19
更新日期:2019-07-23 00:00:00
abstract:UNLABELLED:The newly emerging Middle East respiratory syndrome coronavirus (MERS-CoV) causes a severe respiratory infection with a high mortality rate (~35%). MERS-CoV has been a global threat due to continuous outbreaks in the Arabian peninsula and international spread by infected travelers since 2012. From May to Jul...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00019-16
更新日期:2016-03-01 00:00:00
abstract::Zika virus is a mosquito-borne flavivirus which can cause severe disease in humans, including microcephaly and other congenital malformations in newborns and Guillain-Barré syndrome in adults. There are currently no approved prophylactics or therapeutics for Zika virus; the development of a safe and effective vaccine ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02861-18
更新日期:2019-04-02 00:00:00
abstract::We showed previously that the histone lysine methyltransferase (HKMT) H3K27me3 (EZH2) is the catalytic subunit of Polycomb repressive complex 2 (PRC2) and is required for the maintenance of HIV-1 latency in Jurkat T cells. Here we show, by using chromatin immunoprecipitation experiments, that both PRC2 and euchromatic...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00133-17
更新日期:2017-02-28 00:00:00
abstract::Bats are reservoirs for many RNA viruses that are highly pathogenic in humans yet relatively apathogenic in the natural host. It has been suggested that differences in innate immunity are responsible. The antiviral OAS-RNase L pathway is well characterized in humans, but there is little known about its activation and ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02414-19
更新日期:2019-11-12 00:00:00
abstract::5-Aminosalicylic acid (5-ASA), a peroxisome proliferator-activated receptor gamma (PPAR-γ) agonist, is a widely used first-line medication for the treatment of ulcerative colitis, but its anti-inflammatory mechanism is not fully resolved. Here, we show that 5-ASA ameliorates colitis in dextran sulfate sodium (DSS)-tre...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.03227-20
更新日期:2021-01-19 00:00:00
abstract::Epidemiological studies on Streptococcus pneumoniae show that rates of carriage are highest in early childhood and that the major benefit of the pneumococcal conjugate vaccine (PCV) is a reduction in the incidence of nasopharyngeal colonization through decreased transmission within a population. In this study, we soug...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00188-17
更新日期:2017-03-14 00:00:00