Abstract:
OBJECTIVES:This study examined the antimicrobial susceptibility and resistance mechanisms of Clostridium difficile recovered in Greek hospitals during 2012-2015. METHODS:C. difficile isolates (n=88) were collected from clinically-confirmed C. difficile infection from symptomatic patients in 10 Greek hospitals. Minimum inhibitory concentrations (MICs) of various antimicrobial agents were determined by Etest. Isolates were typed by multilocus sequence typing (MLST). Toxin and resistance genes were detected by PCR. Chromosomal mutations in gyrA, gyrB and rpoB were identified by PCR and sequencing. The genetic environment of resistance genes was characterised by Illumina sequencing. RESULTS:The 88 C. difficile isolates comprised 27 sequence types (STs), with ST37 (n=26) and ST11 (n=21) being the most prevalent. All isolates were susceptible to vancomycin and metronidazole, with variable resistance rates to other antimicrobials. Of the 88 isolates, 45.5% were multidrug-resistant and the majority belonged to ST11 and ST37. The presence of chromosomal mutations in gyrA, gyrB and rpoB was mainly observed in high-risk clones such as ST11 and ST37. The antimicrobial resistance genes ermB, mefA, msrA and tetM were identified at different prevalences and combinations. Additionally, cfrB and cfrC were identified for the first time in Greece and were carried by a Tn6218 transposon and a novel plasmid, respectively. CONCLUSIONS:To our knowledge, this is the first study examining the resistance profiles and respective mechanisms of C. difficile recovered in Greek hospitals. Gut commensals such as C. difficile may serve as hubs for further transfer of antimicrobial resistance genes.
journal_name
J Glob Antimicrob Resistjournal_title
Journal of global antimicrobial resistanceauthors
Chatedaki C,Voulgaridi I,Kachrimanidou M,Hrabak J,Papagiannitsis CC,Petinaki Edoi
10.1016/j.jgar.2018.09.009subject
Has Abstractpub_date
2019-03-01 00:00:00pages
53-58eissn
2213-7165issn
2213-7173pii
S2213-7165(18)30182-6journal_volume
16pub_type
杂志文章abstract:OBJECTIVES:Staphylococcus aureus remains the most important cause of infections in hospitals and long-term care facilities. The aim of this study was to analyse the resistance, virulence, and epidemiological and genetic relationships of S. aureus from bloodstream infections (BSIs) and pneumonia from patients in Souther...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.07.009
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND:OXA-48 is an Ambler class D β-lactamase that hydrolyses penicillin and imipenem but has poor hydrolytic activity against cephalosporins. However, very few clinical experiences of treating extended-spectrum β-lactamase (ESBL)-negative OXA-48 producers with cephalosporins have been published. OBJECTIVES:The a...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.02.016
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVES:Farm animals have been recognised as important carriers and reservoirs of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. The aim of this study was to report the draft genome sequences of two multidrug-resistant (MDR) CTX-M-15-producing E. coli strains (47VL and 13B) isolated from different ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.10.009
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to determine the percentage of antimicrobial-resistant isolates and the associated resistance mechanisms in Gram-negative bacteria from South Western Nigeria. METHODS:A total of 306 non-duplicate unbiased Gram-negative isolates were recovered from patients admitted to three teachin...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.10.016
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVES:Acinetobacter baumannii (A. baumannii) is the world's most significant opportunistic pathogen that causes nosocomial infections; there is widespread health concern due to its ability to acquire multidrug resistance. This study reports a new variety of A. baumannii L13 isolated from Tarim River in Xinjiang Uy...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.05.022
更新日期:2019-09-01 00:00:00
abstract::In the past few years, Europe has experienced an enormous influx of refugees and migrants owing to the ongoing civil war in Syria as well as conflicts, violence and instability in other Asian and African countries. Available data suggest that refugees carry a significant burden of multidrug-resistant (MDR) organisms, ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章,评审
doi:10.1016/j.jgar.2017.03.013
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:Here we describe a clinical isolate of Klebsiella pneumoniae ST11 harbouring both blaKPC-2 and rmtB genes in Japan. METHODS:A carbapenem- and aminoglycoside-resistant K. pneumoniae was isolated from an inpatient in Japan. Whole-genome sequencing (WGS) was performed using an Illumina next-generation sequence...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2018.11.026
更新日期:2019-06-01 00:00:00
abstract::Bacterial resistance is undoubtedly recognised as a major medical challenge in most healthcare systems. Resistance-determining genes, mostly in combination, and multidrug-resistant (MDR) pathogens are spreading with unprecedented speed. Well known resistance carriers with high clinical impact include the Gram-positive...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章,评审
doi:10.1016/j.jgar.2013.03.010
更新日期:2013-06-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to evaluate the role of residue 238 in CTX-M-15 and CTX-M-15G238C mutant with respect to carbapenems and various β-lactamase inhibitors. METHODS:A CTX-M-15G238C laboratory mutant was generated by site-directed mutagenesis from CTX-M-15 enzyme by replacing glycine 238 with cysteine....
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.04.004
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:Escherichia coli and Enterobacter cloacae are two major pathogens causing urinary tract infection (UTI). Here we characterised the genome of an NDM-1-producing E. cloacae strain and an extended-spectrum β-lactamase (ESBL)-producing E. coli strain that were co-isolated from a UTI. METHODS:The genomes of E. c...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.04.007
更新日期:2019-06-01 00:00:00
abstract::Antimicrobial resistance severely limits the therapeutic options for many clinically important bacteria. In Gram-negative bacteria, multidrug resistance is commonly facilitated by plasmids that have the ability to accumulate and transfer refractory genes amongst bacterial populations. The aim of this study was to isol...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2016.01.011
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVES:Multidrug-resistant bacteria have become a serious threat worldwide. In particular, the coexistence of carbapenemase genes and mcr-1 leaves few available treatment options. Here we report a multidrug-resistant Escherichia coli isolate harbouring both mcr-1 and blaNDM-9 from a patient with a urinary tract inf...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2018.10.003
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVES:Streptococcus pneumoniae is an important pathogen in infectious diseases worldwide, and serogroup 6 S. pneumoniae strains are very common in China. This study investigated the serotype distribution, antibiotic resistance patterns and molecular characteristics of 215 serogroup 6 S. pneumoniae isolates collect...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.12.007
更新日期:2018-09-01 00:00:00
abstract::Enterococcus faecium was considered an important nosocomial pathogen that could incur a substantial health burden owing to its high drug resistance and its ability to adapt to the hospital niche. Here we presented the draft genome sequence of multidrug-resistant E. faecium XH877. The draft genome sequence was composed...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2016.07.004
更新日期:2016-12-01 00:00:00
abstract::Acquisition of carbapenemase-producing Klebsiella pneumoniae (CP-Kp) strains poses a major threat to critically ill patients. The objectives of this study were to describe the epidemiology of CP-Kp isolates as well as the clinical outcome associated with the corresponding infections and to identify risk factors for mo...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2015.01.006
更新日期:2015-06-01 00:00:00
abstract:OBJECTIVES:To determine the contribution of specific uropathogenic Escherichia coli (UPEC) lineages, drug resistance genes, and plasmid incompatibility/replicon (Inc) groups to the prevalence of β-lactam-resistant urinary tract infections (UTIs) in a university community. METHODS:Urine samples were consecutively colle...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.03.002
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVES:Pseudomonas aeruginosa is the most frequent infectious agent in cystic fibrosis patients. P. aeruginosa resistance to first line antibiotics limits therapeutic options, but the therapeutic potential of older generation antibiotics, such as fosfomycin is under investigation. Fosfomycin does not belong to any ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.02.014
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVES:This study aimed to characterise insertional mutations disturbing themgrB gene in carbapenem-resistant Klebsiella pneumoniae (CRKp). METHODS:A total of 118 clinical CRKp isolates were surveyed for polymyxin resistance and insertion sequence (IS) elements disruptingmgrB. RESULTS:Of the 118 isolates, 78 (66....
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.11.003
更新日期:2020-11-24 00:00:00
abstract::Antibiotics are frequently used in food animal production in developing countries to promote the well-being and growth of animals. This practice provides some economic benefits to producers and consumers at large. Nevertheless, this practice is also associated with a number of concerns. A major concern has been that r...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章,评审
doi:10.1016/j.jgar.2019.07.031
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVES:Carbapenem-resistant Acinetobacter baumannii (CRAB) have emerged as a serious threat to public-health worldwide. This study aimed to determine the antimicrobial susceptibility of A. baumannii isolates in Iran and to investigate oxacillinase-encoding determinants and their association with insertion sequence ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2018.12.011
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVES:Bacillus spp., which have relevance to pseudo-epidemics in clinics, may cause serious infections as well as high morbidity and mortality in hospitalised patients with haematological malignancies. Here we report the isolation of Bacillus sp. M13(2017) with a multidrug-resistant phenotype and spore-producing s...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2018.07.002
更新日期:2018-09-01 00:00:00
abstract::According to the World Health Organization (WHO), as of today, there are 2.165.500 confirmed cases of the novel coronavirus disease (COVID-19) and 145.705 deaths in over 185 countries. Unfortunately, despite the tremendous efforts to develop a vaccine initiated by various leading health institutions all over the world...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.07.005
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVES:The objective was to investigate how deficient of quorum sensing system (QS) affects minimal inhibitory concentrations (MICs) of Pseudomonas aeruginosa strains to ciprofloxacin (CIP) and levofloxacin (LEV) in vitro. METHODS:Collected the clinical Pseudomonas aeruginosa isolates. Screened the strains deficie...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.04.008
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:Thousands of cases of multidrug-resistant tuberculosis (TB) have been observed in the Philippines, but studies on the Mycobacterium tuberculosis (MTB) genotypes that underlie the observed drug resistance profiles are lacking. This study aimed to analyse the whole genomes of clinical MTB isolates representing...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2018.08.009
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVES:This study describes the characterisation of type 2 IncC plasmids pC-Ec20-KPC and pC-Ec2-KPC, carrying theblaKPC-2 gene, from two multiresistant Escherichia coli recovered in University Hospital of Larissa (Greece) in 2018. METHODS:E. coli strains Ec-2Lar and Ec-20Lar were recovered from rectal swabs of two...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.05.001
更新日期:2019-12-01 00:00:00
abstract::Klebsiella pneumoniae carrying blaCTX-M-15 have been widely disseminated in hospital settings. In this regard, most clinically important strains belong to clonal complex 28 (CC258), which includes sequence type 340 (ST340). In this study, we present the draft genome sequence of a CTX-M-15-producing ST340 K. pneumoniae...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2016.07.012
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVES:The number of multidrug-resistant tuberculosis (MDR-TB) cases is rising worldwide. The present investigation aimed to evaluate, using the GenoType® MTBDRplus assay, the most common mutations associated with rifampicin (RIF) and isoniazid (INH) resistance among resistant strains in Morocco. METHODS:A total o...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.09.017
更新日期:2018-03-01 00:00:00
abstract:OBJECTIVES:Multidrug-resistant (MDR) bacteria are a major public-health concern. Bacteriophage endolysins (lysins) can be used as novel antimicrobial agents against bacterial infections. In this study, a novel endolysin (LysSS) containing a lysozyme-like domain was evaluated for its antibacterial activity against vario...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.01.005
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:Inappropriate antimicrobial prescribing may harm patients and drive antimicrobial resistance. Junior doctors' knowledge of infectious diseases and antimicrobial prescribing is inadequate. Online spaced case-based learning can improve knowledge. OBJECTIVE:To develop infectious diseases and antimicrobial pres...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.02.008
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVES:Recently, the virulence factor sasX was described on the mobile genetic element φSPβ prophage in a ST239-SCCmecIII methicillin-resistant Staphylococcus aureus (MRSA) clone. The aim of this study was to identify sesI, an sasX homologue, in an MRSA ST239 strain. METHODS:MRSA strain VB1490 was isolated from a ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.02.006
更新日期:2019-09-01 00:00:00