Abstract：
OBJECTIVES:This study describes the characterisation of type 2 IncC plasmids pC-Ec20-KPC and pC-Ec2-KPC, carrying theblaKPC-2 gene, from two multiresistant Escherichia coli recovered in University Hospital of Larissa (Greece) in 2018. METHODS:E. coli strains Ec-2Lar and Ec-20Lar were recovered from rectal swabs of two patients during monthly surveillance cultures. Transfer experiments by conjugation were carried out using rifampicin-resistant E. coli A15 laboratory strain as recipient. blaKPC-carrying plasmids were characterised by S1 profiling. Isolates were typed by MLST. Whole-genome sequencing was performed using the Sequel platform. RESULTS:Both E. coli isolates, belonging to ST648, transferred blaKPC-2 to E. coli A15 by conjugation. Plasmid analysis revealed that the transconjugants harboured blaKPC-positive plasmids of different sizes. Analysis of plasmid sequences showed that in both isolates the blaKPC-2 gene was carried on a type 2 IncC plasmid (pC-Ec20-KPC and pC-Ec2-KPC, respectively). Both plasmids carried the ARI-B resistance island consisting of several resistance genes, intact and truncated copies of several mobile elements, and a 25 571-bp segment harbouring coding sequences for an iron transporter. The blaKPC-2 gene was part of transposon Tn4401a, which was bounded by 5-bp direct repeats (TCCTT) suggesting its transposition into the IncC plasmids. CONCLUSION:To our knowledge, this is the first report on complete nucleotide sequences of type 2 IncC plasmids. These findings, which hypothesise the acquisition of KPC-2-encoding transposon Tn4401a by an IncC replicon, indicate the ongoing need for molecular surveillance studies of multidrug-resistant pathogens. In addition, they underline the increasing clinical importance of the IncC plasmid family.
journal_name
J Glob Antimicrob Resistjournal_title
Journal of global antimicrobial resistanceauthors
Papagiannitsis CC,Bitar I,Malli E,Tsilipounidaki K,Hrabak J,Petinaki Edoi
10.1016/j.jgar.2019.05.001subject
Has Abstractpub_date
2019-12-01 00:00:00pages
73-77eissn
2213-7165issn
2213-7173pii
S2213-7165(19)30108-0journal_volume
19pub_type
杂志文章abstract：OBJECTIVES:To determine the contribution of specific uropathogenic Escherichia coli (UPEC) lineages, drug resistance genes, and plasmid incompatibility/replicon (Inc) groups to the prevalence of β-lactam-resistant urinary tract infections (UTIs) in a university community. METHODS:Urine samples were consecutively colle...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.03.002
更新日期：2019-12-01 00:00:00
abstract：:The rapid development of antimicrobial resistance among micro-organisms is a serious public health concern. Moreover, the dissemination of antibiotic-resistant bacteria makes this issue a global problem, and Asia is no exception. For example, since New Delhi metallo-β-lactamase (NDM)-producing Enterobacteriaceae were ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章,评审
doi：10.1016/j.jgar.2014.02.007
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVES:Thousands of cases of multidrug-resistant tuberculosis (TB) have been observed in the Philippines, but studies on the Mycobacterium tuberculosis (MTB) genotypes that underlie the observed drug resistance profiles are lacking. This study aimed to analyse the whole genomes of clinical MTB isolates representing...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.08.009
更新日期：2018-12-01 00:00:00
abstract：:The two closely related mycobacteria, Mycobacterium intracellulare and Mycobacterium chimaera, exhibit a more than two-fold difference in their in vitro susceptibility to sulfonamides. Sulfonamides are antibiotics targeting the 6-hydroxymethyl-7,8-dihydropteroate synthase (DHPS) enzyme involved in the folate synthesis...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2013.05.003
更新日期：2013-12-01 00:00:00
abstract：:Antibiotics are frequently used in food animal production in developing countries to promote the well-being and growth of animals. This practice provides some economic benefits to producers and consumers at large. Nevertheless, this practice is also associated with a number of concerns. A major concern has been that r...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章,评审
doi：10.1016/j.jgar.2019.07.031
更新日期：2020-03-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to validate the use of LML antimicrobial gradient strips for quantitative determination of carbapenem susceptibility in Enterobacteriaceae. METHODS:A total of 95 non-redundant Enterobacteriaceae strains isolated during 2012-2014 were used for this validation study. Initially, LML a...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2016.10.010
更新日期：2017-03-01 00:00:00
abstract：:Antibiotic resistance genes are rapidly spread between pathogens and the normal flora, with plasmids playing an important role in their circulation. This study aimed to investigate antibiotic resistance plasmids in the gut microbiome of hospitalised patients. Stool samples were collected from seven inpatients at Sirir...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2016.03.001
更新日期：2016-09-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to investigate the characteristics and complete genome sequence of an IMP-8, CTX-M-14, CTX-M-3 and QnrS1 co-producing multidrug-resistant Enterobacter asburiae isolate (EN3600) from a patient with wound infection. METHODS:Species identification was confirmed by matrix-assisted lase...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.05.029
更新日期：2019-09-01 00:00:00
abstract：OBJECTIVES:Infections caused by multidrug-resistant Gram-negative bacteria are associated with high mortality. A relevant concern is the efficacy of antibiotic therapy in burn patients in whom pathophysiological changes strongly influence pharmacokinetic (PK) parameters. This study aimed to describe the PK parameters o...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.06.011
更新日期：2020-03-01 00:00:00
abstract：OBJECTIVES:Rates of nosocomial infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) have increased. A meta-analysis was conducted to explore risk factors for CRKP infection in order to provide a theoretical basis for reducing the CRKP infection rate and actively preventing CRKP infection. METHODS:Onl...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.09.006
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVES:Scorpion venoms are a rich source of bioactive peptides with promising clinical value that may lead to the discovery and development of new drugs. The present study was designed to evaluate the in vitro antimicrobial activities of the venoms extracted from three medically important Saudi scorpions (Androcton...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.03.008
更新日期：2017-09-01 00:00:00
abstract：OBJECTIVES:Excessive use of antimicrobial agents for acute upper respiratory infections (URIs) is one of the most common problems in clinical practice. Interventions are required to reduce antimicrobial overuse in these common infections. METHODS:During a 9-month study period (June 2014-February 2015), a quasi-experim...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.08.015
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVES:Bacillus spp., which have relevance to pseudo-epidemics in clinics, may cause serious infections as well as high morbidity and mortality in hospitalised patients with haematological malignancies. Here we report the isolation of Bacillus sp. M13(2017) with a multidrug-resistant phenotype and spore-producing s...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.07.002
更新日期：2018-09-01 00:00:00
abstract：OBJECTIVES:Delafloxacin is a broad-spectrum anionic fluoroquinolone with activity against Gram-positive and Gram-negative organisms, including methicillin-resistant Staphylococcus aureus. Both oral and intravenous formulations were approved for use in the treatment of acute bacterial skin and skin-structure infections ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.09.027
更新日期：2020-12-01 00:00:00
abstract：OBJECTIVES:Marine bivalves can act as bioindicators of marine environment pollution by multidrug-resistant (MDR) enteric bacteria of medical interest. The aim of this study was to report the draft genome sequence of a plasmid-encoded AmpC (pAmpC) (CMY-2)-carrying Escherichia coli isolate recovered from a marine bivalve...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.06.010
更新日期：2018-09-01 00:00:00
abstract：OBJECTIVES:Multidrug-resistant (MDR) enterococci are found extensively in food samples. This study characterized the phenotypic virulence factors and the ability of horizontal gene transfer of a streptomycin resistance gene among enterococci isolated from fermented pork. METHODS:Thirty-six MDR enterococci were subject...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.04.016
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVES:Acinetobacter baumannii (A. baumannii) is the world's most significant opportunistic pathogen that causes nosocomial infections; there is widespread health concern due to its ability to acquire multidrug resistance. This study reports a new variety of A. baumannii L13 isolated from Tarim River in Xinjiang Uy...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.05.022
更新日期：2019-09-01 00:00:00
abstract：OBJECTIVES:Ralstonia pickettii has been increasingly recognised as an emerging opportunistic pathogen in hospital settings in recent years, especially in patients with prolonged hospital stay. Clinical manifestations associated with R. pickettii infection range from mild infections to severe invasive life-threatening i...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.02.020
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVES:Pharmacokinetic (PK) parameters can change significantly during extracorporeal membrane oxygenation (ECMO) and continuous haemodialysis. This case report describes the pharmacokinetics of a 3-h meropenem infusion in an infantile anuric patient on ECMO with continuous haemodialysis. CASE:A 19-month-old femal...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.04.029
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVES:Here we describe a clinical isolate of Klebsiella pneumoniae ST11 harbouring both blaKPC-2 and rmtB genes in Japan. METHODS:A carbapenem- and aminoglycoside-resistant K. pneumoniae was isolated from an inpatient in Japan. Whole-genome sequencing (WGS) was performed using an Illumina next-generation sequence...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.11.026
更新日期：2019-06-01 00:00:00
abstract：:Although molecular methods can now provide fast identification and the antibiotic susceptibility profile of infecting pathogens, these techniques are not affordable by a large majority of clinical laboratories in developing countries and can be considered excessive for simple, community-acquired infections. Most antib...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2013.01.001
更新日期：2013-03-01 00:00:00
abstract：OBJECTIVES:Klebsiella pneumoniae, which exists in the intestinal and respiratory tracts of humans and animals, is an important conditional pathogen in many animals. The aim of the current study was to investigate the antimicrobial resistance profiles and genotypes of extended-spectrum β-lactamase (ESBL)- and AmpC β-lac...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.06.006
更新日期：2017-09-01 00:00:00
abstract：OBJECTIVES:Isoniazid (INH) resistance is a major contributor to the emergence of multidrug resistance in Mycobacterium tuberculosis (MTB), hampering the success of tuberculosis treatment. This study aimed to identify good leads based on INH derivatives against INH-resistant MTB strains. Mutations at codon 315 in the ka...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.06.014
更新日期：2017-12-01 00:00:00
abstract：OBJECTIVES:This meta-analysis was performed to compare polymyxin monotherapy and polymyxin-based combination therapy for carbapenem-resistant Klebsiella pneumoniae (CR-KP) infections. METHODS:We conducted searches on MEDLINE, Embase and Cochrane Collaborative database for both observational studies and randomised cont...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.08.024
更新日期：2020-12-01 00:00:00
abstract：OBJECTIVES:The number of multidrug-resistant tuberculosis (MDR-TB) cases is rising worldwide. The present investigation aimed to evaluate, using the GenoType® MTBDRplus assay, the most common mutations associated with rifampicin (RIF) and isoniazid (INH) resistance among resistant strains in Morocco. METHODS:A total o...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.09.017
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVES:The first report of a plasmid-borne colistin resistance gene (mcr-1) detected in an Escherichia coli isolate from China heralded the emergence of pandrug-resistant bacteria. Since then, the mcr-1 gene has been detected worldwide, but to date it has not been reported in the Philippines. METHODS:In this study...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.12.018
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVES:This study investigated trends and factors associated with antimicrobial resistance (AMR) in Acinetobacter spp. in Europe. METHODS:Using data from EARS-Net, population-weighted multilevel logistic regression models with random intercepts for each participating country were performed to assess trends in Acin...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.05.024
更新日期：2018-09-01 00:00:00
abstract：OBJECTIVES:Antibiotic resistance among Gram-negative pathogens isolated from ventilator-associated pneumonia (VAP) poses a grave threat in intensive care unit (ICU) patients. The aim of this study was to assess the prevalence of pathogens in ICU patients and their drug resistance profile. The prevalence of extended-spe...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2016.12.016
更新日期：2017-06-01 00:00:00
abstract：OBJECTIVES:The widespread dissemination of extended-spectrum β-lactamase-producing Enterobacteriaceae has become a major issue in veterinary medicine. However, until now, there has been no report of bacteria with such a phenotype in infected snakes. The aim of this study was to report the first draft genome sequence of...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.11.009
更新日期：2018-12-01 00:00:00
abstract：OBJECTIVES:This study aimed to isolate and characterise extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E) isolates from animals and wastewater in Tunisia. METHODS:ESBL-E from wastewater (n=123 samples), faeces of healthy animals (poultry, sheep, goats and calves) (n=140) and raw milk from healthy cow...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.01.002
更新日期：2019-06-01 00:00:00
