Abstract：
:Although molecular methods can now provide fast identification and the antibiotic susceptibility profile of infecting pathogens, these techniques are not affordable by a large majority of clinical laboratories in developing countries and can be considered excessive for simple, community-acquired infections. Most antibiotics are therefore prescribed empirically, which tends to avoid those drugs that face high resistance prevalence but that could still be used in a significant number of patients. This is a description of the basis for a fast, cheap method for assessing the presence of bacterial infection and its susceptibility to antibiotics in body fluids that are normally sterile, such as urine. The method is based on detecting a decreased concentration of glucose in liquid media as an indicator of bacterial growth, and the effect of antibiotics upon this change as an indicator of growth arrest by such antibiotics. The method can assess the presence of ca. 105CFU/mL in 4-6h as well as the presence of antibiotic resistance phenotypes of clinical relevance. The method was tested on 20 clinical urine samples, comparing its results with conventional culture and susceptibility assays; results of both the proposed method and the culture/antibiogram were in agreement in all cases. Materials needed for this method would cost no more than US$5 per assay, it would take 10min to set it up, and only requires a small, fixed-temperature incubator. With minor modifications, it could be much more sensitive and easy to run and/or can provide information on presence of specific bacteria.
journal_name
J Glob Antimicrob Resistjournal_title
Journal of global antimicrobial resistanceauthors
Amábile-Cuevas CFdoi
10.1016/j.jgar.2013.01.001subject
Has Abstractpub_date
2013-03-01 00:00:00pages
17-21issue
1eissn
2213-7165issn
2213-7173pii
S2213-7165(13)00002-7journal_volume
1pub_type
杂志文章abstract：:The genus Kluyvera comprises potential pathogens that can cause many infections. This study reports a Kluyvera intermedia strain (FOSA7093) from a pancreatic cyst specimen from a long-term hospitalised patient. Whole-genome sequencing (WGS) of the K. intermedia isolate was performed and the strain was reported as sens...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.05.007
更新日期：2017-09-01 00:00:00
abstract：OBJECTIVES:Escherichia coli and Enterobacter cloacae are two major pathogens causing urinary tract infection (UTI). Here we characterised the genome of an NDM-1-producing E. cloacae strain and an extended-spectrum β-lactamase (ESBL)-producing E. coli strain that were co-isolated from a UTI. METHODS:The genomes of E. c...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.04.007
更新日期：2019-06-01 00:00:00
abstract：OBJECTIVES:Flies have been implicated in the dispersal of medically important bacteria including members of the genus Klebsiella between different environmental compartments. The aim of this study was to retrieve and characterize antibiotic-resistant bacteria from flies collected near to hospitals. METHODS:Flies were ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.11.021
更新日期：2020-12-07 00:00:00
abstract：BACKGROUND:Inappropriate antimicrobial prescribing may harm patients and drive antimicrobial resistance. Junior doctors' knowledge of infectious diseases and antimicrobial prescribing is inadequate. Online spaced case-based learning can improve knowledge. OBJECTIVE:To develop infectious diseases and antimicrobial pres...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.02.008
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to investigate the molecular mechanisms of imipenem resistance in Enterobacteriaceae and to assess the antimicrobial activities of ceftazidime/avibactam (CAZ/AVI) and aztreonam/avibactam (ATM/AVI) against imipenem-resistant clinical isolates in a tertiary hospital in China. METHODS...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.04.023
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to forecast the monthly incidence rates of infections [infections/1000 bed-days (IBD)] due to carbapenem-resistant Klebsiella pneumoniae (CRKP), carbapenem-resistant Pseudomonas aeruginosa (CRPA), carbapenem-resistant Acinetobacter baumannii (CRAB) and total carbapenem-resistant Gra...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.06.019
更新日期：2020-03-01 00:00:00
abstract：OBJECTIVES:Treatment of leishmaniasis remains a challenge, especially due to the need for multiple painful injections, the toxicity of current drugs against the disease, their lack of efficacy and, lately, drug resistance. The aim of this study was to demonstrate the biological behaviour of 3-nitro-2'-hydroxy-4',6'-dim...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.11.012
更新日期：2018-06-01 00:00:00
abstract：OBJECTIVES:The Klebsiella pneumoniae carbapenemase (blaKPC) gene is one of the most widespread carbapenemase genes in the world. However, there are few reports on KPC-producing bacteria in Japan. The aim of this study was therefore to investigate KPC-producing K. pneumoniae in Japan. METHODS:A KPC-2-producingK. pneumo...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.12.007
更新日期：2020-12-26 00:00:00
abstract：OBJECTIVES:Recently, the virulence factor sasX was described on the mobile genetic element φSPβ prophage in a ST239-SCCmecIII methicillin-resistant Staphylococcus aureus (MRSA) clone. The aim of this study was to identify sesI, an sasX homologue, in an MRSA ST239 strain. METHODS:MRSA strain VB1490 was isolated from a ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.02.006
更新日期：2019-09-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to explore the genomic content of a blaKPC-2- and mcr-1-harbouring Escherichia coli strain and to clarify the molecular mechanism for the transmission of multidrug resistance. METHODS:Antimicrobial susceptibility testing was conducted by the broth microdilution method to determine ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.08.023
更新日期：2020-12-01 00:00:00
abstract：OBJECTIVES:Klebsiella pneumoniae, which exists in the intestinal and respiratory tracts of humans and animals, is an important conditional pathogen in many animals. The aim of the current study was to investigate the antimicrobial resistance profiles and genotypes of extended-spectrum β-lactamase (ESBL)- and AmpC β-lac...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.06.006
更新日期：2017-09-01 00:00:00
abstract：OBJECTIVES:Tuberculosis (TB) poses a serious global threat to humans. New bactericidal agents that can shorten treatment duration and target drug resistance still remain a top priority in the discovery of anti-TB drugs. The objective of this study was to investigate the bactericidal potential of 3-cinnamoyl-4-hydroxy-6...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.11.018
更新日期：2020-09-01 00:00:00
abstract：:Antimicrobial resistance severely limits the therapeutic options for many clinically important bacteria. In Gram-negative bacteria, multidrug resistance is commonly facilitated by plasmids that have the ability to accumulate and transfer refractory genes amongst bacterial populations. The aim of this study was to isol...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2016.01.011
更新日期：2016-06-01 00:00:00
abstract：:Enterococcus faecium was considered an important nosocomial pathogen that could incur a substantial health burden owing to its high drug resistance and its ability to adapt to the hospital niche. Here we presented the draft genome sequence of multidrug-resistant E. faecium XH877. The draft genome sequence was composed...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2016.07.004
更新日期：2016-12-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to validate the use of LML antimicrobial gradient strips for quantitative determination of carbapenem susceptibility in Enterobacteriaceae. METHODS:A total of 95 non-redundant Enterobacteriaceae strains isolated during 2012-2014 were used for this validation study. Initially, LML a...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2016.10.010
更新日期：2017-03-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to determine the antimicrobial susceptibility of Neisseria gonorrhoeae isolates in the Lao People's Democratic Republic (Laos). METHODS:A total of 165 gonococcal isolates (1.3%) were obtained from 12 281 genital samples routinely submitted to a diagnostic laboratory in Vientiane, L...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.12.001
更新日期：2018-06-01 00:00:00
abstract：:Bacterial resistance is undoubtedly recognised as a major medical challenge in most healthcare systems. Resistance-determining genes, mostly in combination, and multidrug-resistant (MDR) pathogens are spreading with unprecedented speed. Well known resistance carriers with high clinical impact include the Gram-positive...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章,评审
doi：10.1016/j.jgar.2013.03.010
更新日期：2013-06-01 00:00:00
abstract：OBJECTIVES:The first report of a plasmid-borne colistin resistance gene (mcr-1) detected in an Escherichia coli isolate from China heralded the emergence of pandrug-resistant bacteria. Since then, the mcr-1 gene has been detected worldwide, but to date it has not been reported in the Philippines. METHODS:In this study...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.12.018
更新日期：2020-06-01 00:00:00
abstract：:The objective of this study was to investigate the molecular mechanisms explaining the multidrug-resistant (MDR) phenotype found in a novel clinical Shewanella sp. strain (Shew256) recovered from a diabetic patient. Whole-genome shotgun sequencing was performed using Illumina MiSeq-I and Nextera XT DNA library. De nov...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.06.005
更新日期：2017-12-01 00:00:00
abstract：:Uropathogenic Escherichia coli (UPEC) is the most frequent cause of urinary tract infection (UTI). Virulence factors (VFs) of UPEC in children are not well known. Circulating antibiotic resistance mechanisms in the community are increasing. In this study, the aetiological agents of UTI and antibiotic resistance mechan...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2014.08.003
更新日期：2014-12-01 00:00:00
abstract：OBJECTIVES:Corynebacterium urealyticum is a non-diphtherial urease-producing clinically relevant corynebacterium associated with urinary tract infections. Most clinical C. urealyticum isolates are multidrug-resistant. Whole-genome sequencing (WGS) of C. urealyticum VH4248 isolated from a clinical urine sample at Hospit...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.07.020
更新日期：2020-12-01 00:00:00
abstract：OBJECTIVES:Biofilms associated with implantable medical devices and wounds are clinically relevant, often requiring repeated use of antibiotics without success. A search for non-antibiotic antimicrobial and antibiofilm solutions is warranted, in line with antimicrobial stewardship. Our study aimed to evaluate the broad...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.12.002
更新日期：2020-12-28 00:00:00
abstract：OBJECTIVES:Thousands of cases of multidrug-resistant tuberculosis (TB) have been observed in the Philippines, but studies on the Mycobacterium tuberculosis (MTB) genotypes that underlie the observed drug resistance profiles are lacking. This study aimed to analyse the whole genomes of clinical MTB isolates representing...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.08.009
更新日期：2018-12-01 00:00:00
abstract：:According to the World Health Organization (WHO), as of today, there are 2.165.500 confirmed cases of the novel coronavirus disease (COVID-19) and 145.705 deaths in over 185 countries. Unfortunately, despite the tremendous efforts to develop a vaccine initiated by various leading health institutions all over the world...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.07.005
更新日期：2020-09-01 00:00:00
abstract：:Haemophilus influenzae is an important human pathogen usually susceptible to quinolones. Here we report the emergence of high-level ciprofloxacin-monoresistant H. influenzae in the Region of Southern Denmark. Four isolates were collected for phenotypic and molecular characterisation using whole-genome sequencing (WGS)...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2015.12.004
更新日期：2016-06-01 00:00:00
abstract：OBJECTIVES:This study was conducted to assess the prevalence of azole resistance in Aspergillus isolates from patients with haematological malignancies or who were undergoing haematopoietic stem cell transplantation and to identify the molecular mechanism of resistance. METHODS:In this 28-month prospective study invol...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.01.016
更新日期：2020-09-01 00:00:00
abstract：:Acquisition of carbapenemase-producing Klebsiella pneumoniae (CP-Kp) strains poses a major threat to critically ill patients. The objectives of this study were to describe the epidemiology of CP-Kp isolates as well as the clinical outcome associated with the corresponding infections and to identify risk factors for mo...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2015.01.006
更新日期：2015-06-01 00:00:00
abstract：OBJECTIVES:Carbapenem-resistant Acinetobacter baumannii (CRAB) have emerged as a serious threat to public-health worldwide. This study aimed to determine the antimicrobial susceptibility of A. baumannii isolates in Iran and to investigate oxacillinase-encoding determinants and their association with insertion sequence ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.12.011
更新日期：2019-09-01 00:00:00
abstract：:This study aimed to evaluate the clinical outcomes of vancomycin-resistant enterococcal bloodstream infections (VRE BSI) caused by Enterococcus gallinarum or Enterococcus casseliflavus. Variables associated with treatment failure were determined and treatment options were compared. This was a national retrospective st...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2015.12.002
更新日期：2016-06-01 00:00:00
abstract：OBJECTIVES:Malaria treatment is impeded by increasing resistance to conventional antimalarial drugs. Here we explored the activity of ten novel benzothiophene, thiophene and benzene aminoquinolines. METHODS:In vitro testing was performed by the lactate dehydrogenase assay in chloroquine (CQ)-sensitive Plasmodium falci...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.07.027
更新日期：2020-12-01 00:00:00
