Abstract：
:Brain metastases are the most common intracranial tumors in adults. They usually originate from: lung, breast, renal cell and gastrointestinal cancers, as well as melanoma. Prognosis for brain metastases is still poor and classical treatment combining surgery and radiation therapy should be strongly supported with molecular approaches. However, their successful application depends on a deep understanding of not only genetic, but also epigenetic background of the disease. That will result in an earlier and more precise diagnosis, successful treatment, as well as individualized estimation of clinical outcomes and prognosis. It has already been shown that the epigenetic machinery plays a crucial role in cancer biology, development, and progression. Therefore, we decided to look for metastasis through changes in the most studied epigenetic mark, 5-methylcytosine (m5C) in DNA. We performed global analysis of the m5C contents in DNA isolated from the brain metastatic tumor tissue and peripheral blood samples of the same patients, using thin layer chromatography separation of radioactively labeled nucleotides. We found that the m5C level in DNA from brain metastases: changes in the broad range, overlaps with that of blood, and negatively correlates with the increasing tumor grade. Because the amount of m5C in tumor tissue and blood is almost identical, the genomic DNA methylation can be a useful marker for brain metastases detection and differentiation. Our research creates a scope for future studies on epigenetic mechanisms in neuro-oncology and can lead to development of new diagnostic methods in clinical practice.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Barciszewska AMdoi
10.1042/BSR20180731subject
Has Abstractpub_date
2018-10-22 00:00:00issue
5eissn
0144-8463issn
1573-4935pii
BSR20180731journal_volume
38pub_type
杂志文章abstract：OBJECTIVE:Our aim was to investigate the effect of emodin on intestinal and lung injury induced by acute intestinal injury in rats and explore potential molecular mechanisms. METHODS:Healthy male Sprague-Dawley (SD) rats were randomly divided into five groups (n=10, each group): normal group; saline group; acute intes...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20201605
更新日期：2020-09-30 00:00:00
abstract：:The identification of the sodium potassium pump as a Na+, K+ -ATPase is described. ...
journal_title：Bioscience reports
pub_type： 传,历史文章
doi：10.1023/a:1020196612909
更新日期：1998-08-01 00:00:00
abstract：:The relative proportions of immunoregulatory T-cell subpopulations defined by OKT monoclonal antibodies are disturbed in chronic lymphocytic leukaemia (CLL) patients (even early mild cases) compared with normal subjects in the same age range. The mean ratio OKT4+:OKT8+ (helper:suppressor/cytotoxic) is reversed from th...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01314213
更新日期：1982-08-01 00:00:00
abstract：:Due to poor prognosis of glioblastoma (GBM), there is an urgent need to develop new therapeutic strategies. Besides eliminating GBM tumor cells and stem cells, a novel therapeutic approach aims to target Glioma-associated microglia/macrophages (GAMs). We investigated the molecular profile of GAMs correlated with patie...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20182361
更新日期：2019-06-20 00:00:00
abstract：:Lung surfactant protein A (SP-A) is the most abundant surfactant-associated protein present in the lung. A receptor for SP-A has been shown to be present on A549 alveolar type II cells and on other cell types, including alveolar macrophage. The SP-A receptor on A549 cells has been identified as the collection receptor...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01145960
更新日期：1993-04-01 00:00:00
abstract：:In order to gain information on the metabolism of dolichol, the rates of excretion of dolichol and dolichyl phosphate were determined in rats maintained on a dolichol-free diet for 10 days. Analysis of fecal samples collected every 48 h yielded mean output values of 9.0 +/- 1.4 and 20.7 +/- 3.0 micrograms/rat/day of d...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01128818
更新日期：1984-09-01 00:00:00
abstract：:Canstatin, the non-collagenous domain of collagen type IV α-chains, belongs to a series of collagen-derived angiogenic inhibitors. In this study, the inhibitory effect of recombinant canstatin on tumour growth was investigated using a gastric cancer xenograft model. The volume and weight of tumours in mice treated wit...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20140012
更新日期：2014-04-01 00:00:00
abstract：:Polymorphisms of methylenetetrahydrofolate reductase (MTHFR) in hormone metabolism pathways might cause metabolic disturbances and contribute to the development of polycystic ovary syndrome (PCOS) and ovarian cancer, but the published studies were inconsistent. The aim of the present study was to evaluate the MTHFR C6...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200995
更新日期：2020-07-31 00:00:00
abstract：:Imipramine and clorgyline, at concentrations of 0.002 M, inhibit the respiration of brain tissue by 82 and 71 per cent respectively, while chloropromazine and tranylcypromine, at concentrations of 0.01 M, inhibit it about 25 per cent. Deprenyl and amphetamine at a concentration of 0.002 M inhibit brain tissue respirat...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01118600
更新日期：1991-02-01 00:00:00
abstract：:Prognostic significance of family with sequence similarity 83, member D (FAM83D) in hepatocellular carcinoma (HCC) patients has not been well-investigated using Gene Expression Omnibus (GEO) series and TCGA database, we compared FAM83D expression levels between tumor and adjacent tissues, and correlated FAM83D in tumo...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20181640
更新日期：2019-05-07 00:00:00
abstract：:Background: Ki67 has been a key role for the treatment options and prognosis evaluation in some kinds of tumors; however, the spatial expression of Ki67 in oral squamous cell carcinoma (OSCC) has not been fully-evaluated. Therefore, in the present study, we aimed to elucidate the prognosis value of Ki67 spatial expres...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20181271
更新日期：2018-11-20 00:00:00
abstract：:Escherichia coli accumulates K+ by means of multiple transport systems, of which TrkA is the most prominent at neutral and alkaline pH while Kup is major at acidic pH. In the present study, K+ uptake was observed with cells grown under fermentative conditions at an initial pH of 9.0 and 7.3 (the medium pH decreased to...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1023/a:1026493024066
更新日期：2000-08-01 00:00:00
abstract：:The affinity of epigallocatechin gallate (EGCG) for human serum albumin (HSA) was measured in physiological conditions using NMR and isothermal titration calorimetry (ITC). NMR estimated the Ka (self-dissociation constant) of EGCG as 50 mM. NMR showed two binding events: strong (n1=1.8 ± 0.2; Kd1 =19 ± 12 μM) and weak...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20170209
更新日期：2017-05-11 00:00:00
abstract：:Microvirin (MVN) is a carbohydrate-binding protein which shows high specificity for high-mannose type N-glycan structures. In the present study, we tried to identify whether MVN could bind to high-mannose containing hepatitis C virus (HCV) envelope glycoproteins, which are heavily decorated high-mannose glycans. In ad...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20170015
更新日期：2017-06-30 00:00:00
abstract：:NMR is ideal for characterizing non-enzymatic protein glycation, including AGEs (advanced glycation endproducts) underlying tissue pathologies in diabetes and ageing. Ribose, R5P (ribose-5-phosphate) and ADPR (ADP-ribose), could be significant and underinvestigated biological glycating agents especially in chronic inf...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20130135
更新日期：2014-04-01 00:00:00
abstract：:Using both immunohistological study and photoaffinity labelling with radioactive azido-ATP, evidence is presented that the mitochondrial membranes of the brown adipose tissue of the human adult contain a 32 000-Mr uncoupling protein, which is probably similar to the uncoupling protein of BAT of rodents. ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01120989
更新日期：1983-08-01 00:00:00
abstract：:Wilms tumor is the most common renal malignancy that occurs in children. TP53 gene is considered as a tumor-suppressing gene through controlling cell growth. TP53 gene rs1042522 C>G (Arg72Pro) polymorphism is widely investigated in various types of cancers. However, it is not established if TP53 rs1042522 C>G polymorp...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20181891
更新日期：2019-01-22 00:00:00
abstract：:In a study of Necturus gallbladder epithelium Benzel et al. (Benzel et al., 1980) found that low (0.2-1.2 microM) and higher concentrations (1.5 microM and more) of cytochalasin B (CB) caused an increase and decrease in the transepithelial electrical resistance (TER), respectively. Moreover, there were slight changes ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01855015
更新日期：1996-08-01 00:00:00
abstract：:Prostate cancer (PCa), the second most mortal cancer from developed countries, presents a high level of chemoresistance. There is emerging evidence underscores the critical role of autophagy in the onset, progression, and chemoresistance of PCa. In the present study, we investigated the possible role of a novel autoph...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20170770
更新日期：2019-08-23 00:00:00
abstract：:Gram negative bacterial infection is a leading cause of fatality and is attributed, at least in part, to the bacteria's capacity to persist in the host in spite of appropriate antibiotic therapy. It has been suggested that bacteria evade antibiotics by hiding within host cells. We sought to investigate this important ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1023/a:1020216323271
更新日期：1999-10-01 00:00:00
abstract：:Understanding cell migration in a 3D microenvironment is essential as most cells encounter complex 3D extracellular matrix (ECM) in vivo Although interactions between cells and ECM have been studied previously on 2D surfaces, cell migration studies in 3D environment are still limited. To investigate cell migration und...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20181596
更新日期：2019-02-19 00:00:00
abstract：:PU box binding protein (PU.1) is a critical transcription factor involved in many pathological processes. However, its exact role in activation of hepatic stellate cells (HSCs) and liver fibrosis was rarely reported. Here, we found that, in HSCs of PU.1+/- mice, Sirt1 mRNA expression was not changed but Sirt1 protein ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20170926
更新日期：2017-12-12 00:00:00
abstract：:Long noncoding RNA (lncRNA) differentiation antagonizing nonprotein coding RNA (DANCR) plays important regulatory roles in many solid tumors. However, the effect of DANCR in glioma progression and underlying molecular mechanisms were not entirely explored. In the present study, we determined the expression of DANCR in...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20171664
更新日期：2018-02-02 00:00:00
abstract：:The concentration of Na+ and K+ and the pH in the cytoplasm of Lettré cells was measured by monitoring the net flux of H+, Na+, or K+ across the plasma membrane which had been rendered permeable to these ions by the action of Sendai virus. Ion flux was measured directly by analysis of cell composition, or indirectly b...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01172873
更新日期：1983-07-01 00:00:00
abstract：:Fungal endophytes are known to secrete a large array of secondary metabolites (phenols, flavonoids, indole acetic acid (IAA) etc.) that facilitate crops under stress conditions. Considering this, a potent plant growth promoting endophyte (SXSp1) from the spines and leaves of Solanum xanthocarpum L. has been isolated. ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20190290
更新日期：2019-10-30 00:00:00
abstract：:The sodium contents of beta-cell-rich pancreatic islets from ob/ob-mice were measured with an integrating flame photometer. After washing to an apparent steady state with different types of ice-cold media, islets incubated in the absence of glucose contained 79-108 mmol sodium kg-1 dry weight. Exposure to glucose resu...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01114973
更新日期：1986-11-01 00:00:00
abstract：:Matrix-bound monoclonal antibodies against urokinase have been used to purify this enzyme by affinity chromatography. In a single-step procedure, urokinase can be isolated from crude preparations with high yield and high purity, and without loss of enzymatic activity. ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01122902
更新日期：1983-04-01 00:00:00
abstract：:A model explaining quantal Ca2+ release as an intrinsic property of the inositol 1,4,5-triphosphate (IP3) receptor has been put forward. The model is based on the hypothesis that the IP3 receptor can catalyze a transformation of the IP3 molecule differing from its conventional metabolism. A simple kinetic mechanism is...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01207265
更新日期：1996-10-01 00:00:00
abstract：:Bifunctional dihaem cytochrome c thiosulfate dehydrogenases/tetrathionate reductases (TsdA) exhibit different catalytic properties depending on the source organism. In the human food-borne intestinal pathogen Campylobacter jejuni, TsdA functions as a tetrathionate reductase enabling respiration with tetrathionate as a...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20160457
更新日期：2016-12-09 00:00:00
abstract：:The network meta-analysis was conducted to compare the short-term efficacy of different single-drug targeted therapies in the treatment of renal cell carcinoma (RCC). We initially searched databases for randomized controlled trials (RCTs) on different single-drug targeted therapies in treating RCC. The meta-analysis c...
journal_title：Bioscience reports
pub_type： 杂志文章,meta分析
doi：10.1042/BSR20170827
更新日期：2017-12-07 00:00:00
