Abstract:
:NMR is ideal for characterizing non-enzymatic protein glycation, including AGEs (advanced glycation endproducts) underlying tissue pathologies in diabetes and ageing. Ribose, R5P (ribose-5-phosphate) and ADPR (ADP-ribose), could be significant and underinvestigated biological glycating agents especially in chronic inflammation. Using [U-13C]ribose we have identified a novel glycoxidation adduct, 5-deoxy-5-desmethylpronyl-lysine, 'norpronyl-lysine', as well as numerous free ketones, acids and amino group reaction products. Glycation by R5P and ADPR proceeds rapidly with R5P generating a brown precipitate with PLL (poly-L-lysine) within hours. ssNMR (solid-state NMR) 13C-13C COSY identifies several crosslinking adducts such as the newly identified norpronyl-lysine, in situ, from the glycating reaction of 13C5-ribose with collagen. The same adducts are also identifiable after reaction of collagen with R5P. We also demonstrate for the first time bio-amine (spermidine, N-acetyl lysine, PLL) catalysed ribose 2-epimerization to arabinose at physiological pH. This work raises the prospect of advancing understanding of the mechanisms and consequences of glycation in actual tissues, in vitro or even ex vivo, using NMR isotope-labelled glycating agents, without analyses requiring chemical or enzymatic degradations, or prior assumptions about glycation products.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Bullock PT,Reid DG,Ying Chow W,Lau WP,Duer MJdoi
10.1042/BSR20130135subject
Has Abstractpub_date
2014-04-01 00:00:00issue
2eissn
0144-8463issn
1573-4935pii
BSR20130135journal_volume
34pub_type
杂志文章abstract::Mitochondrial dysfunction causes a range of early-onset neurological diseases and contributes to neurodegenerative conditions. The mechanisms of neurological damage however are poorly understood, as accessing relevant tissue from patients is difficult, and appropriate models are limited. Hence, we assessed mitochondri...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140151
更新日期:2014-11-21 00:00:00
abstract:BACKGROUND:Glioma is the most common malignant tumor in the human central nervous system. Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) promotes oncogenesis in various tumors. In the present study, we aimed to examine the role of NEAT1 in altering the properties of gliomas. METHODS:Quantitative ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200767
更新日期:2021-01-04 00:00:00
abstract::Significant amounts of somatostatin-like immunoreactivity (SLI) were detected in the extract of a human catecholamine-secreting adrenal medullary tumour. After salt fractionation and reconstitution the major portion of SLI was purified by gel filtration and two HPLC steps; in all three systems it eluted in the positio...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116377
更新日期:1982-03-01 00:00:00
abstract::Ovarian cancer is a highly lethal cancer in females. Therefore, it is necessary to explore effective biomarkers for the diagnosis and prognosis of the disease. Stratifin (SFN) is a cell cycle checkpoint protein that has been reported to be involved in oncogenesis. Our studies detected the expression of SFN in ovarian ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190100
更新日期:2019-05-02 00:00:00
abstract::Recent experimental evidence increasingly shows that the dysregulation of cellular bioenergetics is associated with a wide array of common human diseases, including cancer, neurological diseases and diabetes. Respiration provides a vital source of cellular energy for most eukaryotic cells, particularly high energy dem...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160083
更新日期:2016-06-30 00:00:00
abstract::Liver cancer is now one of the most lethal and commonest cancers in the world, among which over 90% is hepatocellular carcinoma (HCC). Recent studies have confirmed long non-coding RNAs (lncRNAs) are implicated in carcinogenesis. It has been reported lncRNA LINC00668 serves as an oncogene in several cancers. However, ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20192697
更新日期:2020-05-29 00:00:00
abstract::PU box binding protein (PU.1) is a critical transcription factor involved in many pathological processes. However, its exact role in activation of hepatic stellate cells (HSCs) and liver fibrosis was rarely reported. Here, we found that, in HSCs of PU.1+/- mice, Sirt1 mRNA expression was not changed but Sirt1 protein ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170926
更新日期:2017-12-12 00:00:00
abstract::The exon junction complex (EJC) is the main mechanism by which cells select specific mRNAs for translation into protein. We hypothesized that the EJC is involved in the regulation of gene expression during the stress response in cardiac myocytes, with implications for the failing heart. In cultured rat neonatal myocyt...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170707
更新日期:2017-07-07 00:00:00
abstract::The marbled rockfish Sebastiscus marmoratus is an ecologically and economically important marine fish species distributed along the northwestern Pacific coast from Japan to the Philippines. Here, next-generation sequencing was used to generate a whole genome survey dataset to provide fundamental information of its gen...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20192252
更新日期:2020-02-28 00:00:00
abstract::Accumulating evidence demonstrates that long non-coding RNAs (LncRNAs) play important roles in regulating gene expression and are involved in various cancers, including colorectal cancer (CRC). However, LncRNA profiles in CRC remain largely unknown. The present study aims to find the key LncRNA associated with CRC and...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160048
更新日期:2016-05-06 00:00:00
abstract::Objective: We aimed to assess the possible relations between serum levels of macrophage migration inhibitory factor (MIF), a central cytokine of the innate immunity and inflammatory response, and benign paroxysmal positional vertigo (BPPV) risk and BPPV recurrence events.Methods: In the present study, 154 patients wit...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20191831
更新日期:2019-08-23 00:00:00
abstract::Sendai virus envelopes (SVE) were isolated from Sendai virus particles by Triton X-100 solubilization and ultracentrifugation. The envelopes were reconstituted in the presence of the fluorescent dye calcein by gradual removal of the detergent with Bio-beads SM-2. The internal volume of reconstituted Sendai virus envel...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121911
更新日期:1984-07-01 00:00:00
abstract::Even though red blood cell (RBC) vesiculation is a well-documented phenomenon, notably in the context of RBC aging and blood transfusion, the exact signalling pathways and kinases involved in this process remain largely unknown. We have established a screening method for RBC vesicle shedding using the Ca(2+) ionophore...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20150019
更新日期:2015-04-16 00:00:00
abstract::The present study estimates the effect of rivaroxaban on preventing deep vein thrombosis (DVT) in aged diabetics with femoral neck fractures after hip replacement. Our study consisted of 236 aged diabetics with femoral neck fractures, which were divided into the rivaroxaban and control groups. Reaction time (R time), ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170289
更新日期:2017-06-21 00:00:00
abstract::PDC (pyruvate dehydrogenase complex) is a multi-enzyme complex comprising an E1 (pyruvate decarboxylase), an E2 (dihydrolipomide acetyltransferase) and an E3 (dihydrolipoamide dehydrogenase). PDC catalyses the decarboxylation of pyruvate and forms acetyl-CoA and NADH. In the human malaria parasite Plasmodium falciparu...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140150
更新日期:2015-01-14 00:00:00
abstract::Chemical and physical stimuli trigger a cutaneous response by first inducing the main epidermal cells, keratinocytes, to produce specific mediators that are responsible for the initiation of skin inflammation. Activation modulates cell communication, namely leucocyte recruitment and blood-to-skin extravasation through...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20070012
更新日期:2008-02-01 00:00:00
abstract::Stimulatory GTP-binding Protein (Gs) and adenylate cyclase prepared from bovine brain cortices were co-reconstituted into asolectin vesicles with or without 1000-fold transmembrane Ca2+ gradient. The results showed that both basal activity and Gs-stimulated activity of adenylate cyclase were highest in proteoliposomes...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01200247
更新日期:1994-08-01 00:00:00
abstract::Partitioning in charge-sensitive dextran-poly(ethylene glycol) aqueous phase systems reveals that fixation with even small concentrations of glutaraldehyde (e.g., 0.1% w/v) changes the surface properties of cells. While fixation with larger concentrations of glutaraldehyde (i.e., 1.85%) increases erythrocyte partition...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114811
更新日期:1989-12-01 00:00:00
abstract::Radioactivity from [14C] adenosine was linearly incorporated into tissue nucleotides in perfused rat hearts. All the TCA-extractable 14C was confined to the purine nucleoside phosphates for up to 1 h of perfusion. Radioactivity was also incorporated linearly into the TCA-insoluble fraction, which by 40 min accounted f...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114819
更新日期:1981-11-01 00:00:00
abstract::The coupling of the chemical reaction of ATP hydrolysis to the transport of calcium from the cytoplasm into the lumen of sarcoplasmic reticulum vesicles can be defined by a set of rules that define alternating changes in the specificities of the enzyme for catalysis of chemical and physical reactions. ...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/BF01788360
更新日期:1995-10-01 00:00:00
abstract::In plants, the hydroxymethylpyrimidine (HMP) and thiazole precursors of thiamin are synthesized and coupled together to form thiamin in plastids. Mutants unable to form HMP can be rescued by exogenous HMP, implying the presence of HMP transporters in the plasma membrane and plastids. Analysis of bacterial genomes reve...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180048
更新日期:2018-03-29 00:00:00
abstract::When the amino-acid sequence of the 108-residue, rat tumour calcium-binding protein, oncomodulin, was aligned with that of rat muscle parvalbumin, 55 homologous positions were found, with an additional 33 single base-pair substitutions. This extensive homology, with virtual identity of the calcium-binding domains, sig...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121034
更新日期:1983-11-01 00:00:00
abstract::Although radioactive iodine (131I) treatment (RIT) is recommended as the preferred option for patients with Graves' disease (GD), the predictive factors for its clinical outcomes are still unclear. In the present study, we aim to investigate the factors influencing the success rate of RIT treatment on primary GD with ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20191609
更新日期:2020-01-31 00:00:00
abstract::Background: Many epidemiologic studies have explored the association between age at last birth (ALB) and the risk of epithelial ovarian cancer, but the results remain controversial. Methods: A literature search was performed in PubMed, Web of Science, China National Knowledge Infrastructure (CNKI) and WanFang Med Onli...
journal_title:Bioscience reports
pub_type: 杂志文章,meta分析
doi:10.1042/BSR20182035
更新日期:2019-09-13 00:00:00
abstract::The emergence of multidrug-resistant (MDR) microbes caused by overuse of antibiotics leads to urgent demands for novel antibiotics exploration. Our recent data showed that Ly2.1-3 (a novel lymphocyte antigen 6 (Ly6) gene cluster) were proteins with cationic nature and rich in cysteine content, that are characteristic ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160265
更新日期:2017-01-17 00:00:00
abstract::HIV-1 infects host cells by sequential interactions of its fusion protein (gp120-gp41) with receptors CD4, CXCR4 and/or CCR5 followed by fusion of viral and host membranes. Studies indicate that additional factors such as receptor density and composition of viral and cellular lipids can dramatically modulate the fusio...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/s10540-005-2894-5
更新日期:2005-10-01 00:00:00
abstract::Inactivation of p53 is one of the most relevant events in human cancer, since it allows transformed cells to escape their own proliferation control and leave them irresponsive to drugs that aim to damage their DNA. When p53 falls, other members of its family may become targets to attack tumoural cells. p73 has shown c...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20150039
更新日期:2015-04-28 00:00:00
abstract::LncRNA PACER is a chondrocyte inflammation-associated long non-coding RNA (lncRNA), and chondrocyte inflammation is involved in osteoarthritis (OA). We observed that plasma PACER was down-regulated, while plasma HOTAIR was up-regulated in OA patients. Altered plasma levels of PACER and HOTAIR distinguished OA patients...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190404
更新日期:2019-06-07 00:00:00
abstract::Osteoarthritis (OA) is the most common form of arthritis, and connective tissue growth factor (CTGF) is found to be up-regulated in adjacent areas of cartilage surface damage. CTGF is present in osteophytes of late stage OA. In the present study, we have reviewed association of CTGF in the development and progression ...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1042/BSR20191374
更新日期:2019-07-18 00:00:00
abstract::Bleaching of the purple membrane strongly reduces the number of divalent cation binding sites as well as their affinities. Conversely, deionization of the bleached membrane drastically inhibits the chromophore regeneration. Proteolysis experiments using bromelain show that the bleached membrane has an additional cleav...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114972
更新日期:1986-11-01 00:00:00