Abstract：
:A rapid multiplex silver enhanced 'sandwich vertical flow immunogold assay (SVIA)' based on disposable porous filter-membrane was developed for on-site detection of oxytetracycline (OTC) residues in fish tissues. Artificial antigens were synthesized to raise selective anti-OTC rabbit antiserum and highly specific monoclonal antibody (mAb). The assay consists of three layers. The first layer was composed of immobilized anti-OTC rabbit antiserum (capture antibody) onto the membrane, the second layer was the molecule of interest (OTC) and the third layer was refined with detector immunogold labeled mAb. The sensitivity of the silver enhanced SVIA was as low as 2 ng mL-1 (within 4 min) representing a 125-fold increase in sensitivity over the HRP labeled sandwich vertical flow assay. The reliability of developed technique was co-evaluated with HPLC and validated using 115 field fish samples from different regions of southern India. The results demonstrated that SVIA has the high potentiality to be established as a semi-quantitative routine on-site screening tool.
journal_name
Food Chemjournal_title
Food chemistryauthors
Moumita M,Shankar KM,Abhiman PB,Shamasundar BAdoi
10.1016/j.foodchem.2018.07.124subject
Has Abstractpub_date
2019-01-01 00:00:00pages
585-592eissn
0308-8146issn
1873-7072pii
S0308-8146(18)31272-Xjournal_volume
270pub_type
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