Abstract：
:Food analysis to ensure food safety and quality are relevant to all countries. This study aimed to develop a detection technique by combining recombinase polymerase amplification with CRISPR-Cas12a for food safety (termed RPA-Cas12a-FS). Our data showed that this novel method could be detected via fluorescence intensity for the molecular identification of foodborne pathogenic bacteria, genetically modified crops, and meat adulteration. After optimization, the sensitivity and stability of RPA-Cas12a-FS was further enhanced. The RPA-Cas12a-FS system could specifically detect target gene levels as low as 10 copies in 45 min at 37 °C. The RPA-Cas12a-FS system was sensitive both using standard samples in the lab and using samples from the field, which indicated that this detection method was practical. In conclusion, a simple, rapid, and highly sensitive detection method based on CRISPR-Cas12a was developed for molecular identification in the food safety field without requiring technical expertise or ancillary equipment.
journal_name
Food Chemjournal_title
Food chemistryauthors
Liu H,Wang J,Zeng H,Liu X,Jiang W,Wang Y,Ouyang W,Tang Xdoi
10.1016/j.foodchem.2020.127608subject
Has Abstractpub_date
2021-01-01 00:00:00pages
127608eissn
0308-8146issn
1873-7072pii
S0308-8146(20)31470-9journal_volume
334pub_type
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