A rapid, sensitive and accurate determination of cobalamin with double monitoring system: HPLC-UV and HPLC-ICP-OES.

Abstract:

:This study proposed a novel analytical method for the separation and determination of cobalamin and cobalt in kefir samples by high performance liquid chromatography-inductively coupled plasma-optical emission spectrometry (HPLC-ICP-OES) in addition to determination of cobalamin in HPLC system. Chromatographic parameters such as column type, buffer solution, mobile phase flow rate and sample injection volume were individually studied and optimized. In addition, cobalamin was simultaneously determined by high performance liquid chromatography with ultraviolet detection (HPLC-UV). LOD values of cobalt in cobalamin and cobalt for HPLC-ICP-OES system were calculated as 0.07 mg/kg (as Co) and 0.06 mg/kg, respectively. Recovery studies were conducted to evaluate the accuracy/applicability of the method. Recovery results for cobalt in cobalamin and cobalt detected by the HPLC-ICP-OES system were calculated in the range of 87.4-100.1 and 98.8-115.0%, respectively while recovery results for cobalamin were found to be between 89.2 and 98.3% for HPLC-UV system.

journal_name

Food Chem

journal_title

Food chemistry

authors

Bodur S,Erarpat S,Balçık U,Bakırdere S

doi

10.1016/j.foodchem.2020.127945

subject

Has Abstract

pub_date

2021-03-15 00:00:00

pages

127945

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(20)31807-0

journal_volume

340

pub_type

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