Abstract：
:An efficient and reliable duplex SYBR Green real-time quantitative PCR (qPCR) method for beef products adulteration detection was developed based on bovine specific and vertebrate universal primers. By analyzing the numbers, positions (Tm value) of melting curve peaks of the duplex PCR products, we simultaneously identified bovine and preliminary screened non-bovine in samples, and also semi-quantified the bovine percentage according to the area ratios of peaks. All of these were necessary for adulteration determination. The specific and universal primers were designed based on mitochondrial genes ND4 and 16S rRNA respectively, their amplicons Tm values were 72.6 ± 0.5 °C and 79-81 °C. There might be some other peaks at 74-78 °C and above 81 °C if non-bovine components existed. Thelimit of detectionwas 1 pgforbovineDNA, and1 - 30 pg fornon-bovineDNAbasedon differentspecies.
journal_name
Food Chemjournal_title
Food chemistryauthors
Li J,Wei Y,Li J,Liu R,Xu S,Xiong S,Guo Y,Qiao X,Wang Sdoi
10.1016/j.foodchem.2020.127932subject
Has Abstractpub_date
2021-02-15 00:00:00pages
127932eissn
0308-8146issn
1873-7072pii
S0308-8146(20)31794-5journal_volume
338pub_type
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