Abstract：
:The anti-metabolic or insecticidal gene, arcelin (Arl) was isolated, cloned and sequenced using sequence specific degenerate primers from the seeds of Lablab purpureus collected from the Western Ghats, Tamil Nadu, India. The L. purpureus arcelin nucleotide sequence was homologous to Arl-3 and Arl-4 alleles from Phaseolus spp. The protein it encodes has 70% amino acid identity with the amino acid sequences of Arl-3I, Arl-3III, Arl-4 precursor, Arl-4 and Arl-4I. The partially purified arcelin from the seeds of L. purpureus using an artificial diet confirmed the complete retardation of development of the stored product pest Callosobruchus maculatus at 0.2% w/w arcelin-incorporated artificial seeds.
journal_name
Food Chemjournal_title
Food chemistryauthors
Janarthanan S,Sakthivelkumar S,Veeramani V,Radhika D,Muthukrishanan Sdoi
10.1016/j.foodchem.2012.06.129subject
Has Abstractpub_date
2012-12-15 00:00:00pages
2839-44issue
4eissn
0308-8146issn
1873-7072pii
S0308-8146(12)01200-9journal_volume
135pub_type
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