Comparative proteomic analysis of two clam species: Chamelea gallina and Tapes philippinarum.

Abstract:

:Clams have long been a fisheries and aquaculture sector of great importance in Italy, the main resource of fisheries is the Chamelea gallina of indigenous origin, whereas clams breeding is supported almost entirely by the Tapes philippinarum, a species of Indo-Pacific origin. Bivalve molluscs quality depends mainly on the water quality, and then by a series of factors such as water temperature and salinity, gametogenic cycle, food availability, and environmental conditions, that affect the Condition Index. In this work crude extracts obtained from the edible part of Chamelea gallina and Tapes philippinarum were analyzed by a proteomic approach based on a two-dimensional gel electrophoresis followed by liquid chromatography-tandem mass spectrometry, in order to detect biomarkers useful for identification of the two kinds of clams and to assess their nutritional characteristics. As a result, four differentially expressed spots were found and identified, namely enolase, cyclophilin-A, ribosomal protein L13 and actin-1.

journal_name

Food Chem

journal_title

Food chemistry

authors

Vincenzetti S,Felici A,Ciarrocchi G,Pucciarelli S,Ricciutelli M,Ariani A,Polzonetti V,Polidori P

doi

10.1016/j.foodchem.2016.09.150

subject

Has Abstract

pub_date

2017-03-15 00:00:00

pages

223-229

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(16)31549-7

journal_volume

219

pub_type

杂志文章