Biochemical and pharmacological characterization of an extremely potent and selective nonpeptide cholecystokinin antagonist.

abstract：:Terminal oligopyrimidine (TOP) motifs are sequences at the 5' ends of mRNAs that link their translation to the mTOR Complex 1 (mTORC1) nutrient-sensing signaling pathway. They are commonly regarded as discrete elements that reside on ∼100 mRNAs that mostly encode translation factors. However, the full spectrum of TOP ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Philippe L,van den Elzen AMG,Watson MJ,Thoreen CC

更新日期：2020-03-10 00:00:00

abstract：:Liddle syndrome is a mendelian form of hypertension characterized by constitutively elevated renal Na reabsorption that can result from activating mutations in the beta or gamma subunit of the epithelial Na channel. All reported mutations have deleted the last 45-76 normal amino acids from the cytoplasmic C terminus o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hansson JH,Schild L,Lu Y,Wilson TA,Gautschi I,Shimkets R,Nelson-Williams C,Rossier BC,Lifton RP

更新日期：1995-12-05 00:00:00

abstract：:Kabuki syndrome is a Mendelian intellectual disability syndrome caused by mutations in either of two genes (KMT2D and KDM6A) involved in chromatin accessibility. We previously showed that an agent that promotes chromatin opening, the histone deacetylase inhibitor (HDACi) AR-42, ameliorates the deficiency of adult neur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benjamin JS,Pilarowski GO,Carosso GA,Zhang L,Huso DL,Goff LA,Vernon HJ,Hansen KD,Bjornsson HT

更新日期：2017-01-03 00:00:00

abstract：:Many animals employ sex pheromones to find mating partners during their reproductive seasons. However, most sex pheromones of vertebrates remain to be identified. Over the past 20 years, steroids and prostaglandins have been identified as sex pheromones in several fishes. These pheromones are broadly termed "hormonal ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yambe H,Kitamura S,Kamio M,Yamada M,Matsunaga S,Fusetani N,Yamazaki F

更新日期：2006-10-17 00:00:00

abstract：:Solution at 2.5-A resolution of the three-dimensional structure of H-2Ld with a single nine-residue peptide provides a structural basis for understanding its unique interaction with beta-2 microglobulin (beta2m) and peptide. Consistent with the biological data that show an unusually weak association of Ld with beta2m,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balendiran GK,Solheim JC,Young AC,Hansen TH,Nathenson SG,Sacchettini JC

更新日期：1997-06-24 00:00:00

abstract：:Spontaneous reciprocal mitotic recombination in the yeast Saccharomyces cerevisiae, associated with heteroallelic recombination, occurs almost exclusively at the two-strand stage and involves recombination of unduplicated chromosomes (i.e., during G1) or the unduplicated regions of chromosomes during the S phase of mi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Esposito MS

更新日期：1978-09-01 00:00:00

abstract：:Gene regulatory networks (GRNs) provide a transformation function between the static genomic sequence and the primary spatial specification processes operating development. The regulatory information encompassed in developmental GRNs thus goes far beyond the control of individual genes. We here address regulatory info...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peter IS,Davidson EH

更新日期：2017-06-06 00:00:00

abstract：:From an analysis of the distributions of measures of transmission rates among hosts, we identify an empirical relationship suggesting that, typically, 20% of the host population contributes at least 80% of the net transmission potential, as measured by the basic reproduction number, R0. This is an example of a statist...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Woolhouse ME,Dye C,Etard JF,Smith T,Charlwood JD,Garnett GP,Hagan P,Hii JL,Ndhlovu PD,Quinnell RJ,Watts CH,Chandiwana SK,Anderson RM

更新日期：1997-01-07 00:00:00

abstract：:Although cells express hundreds of metalloenzymes, the mechanisms by which apoenzymes receive their metal cofactors are largely unknown. Poly(rC)-binding proteins PCBP1 and PCBP2 are multifunctional adaptor proteins that bind iron and deliver it to ferritin for storage or to prolyl and asparagyl hydroxylases to metall...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frey AG,Nandal A,Park JH,Smith PM,Yabe T,Ryu MS,Ghosh MC,Lee J,Rouault TA,Park MH,Philpott CC

更新日期：2014-06-03 00:00:00

abstract：:Agrobacterium tumefaciens causes crown gall tumors on various plants by delivering transferred DNA (T-DNA) and virulence proteins into host plant cells. Under laboratory conditions, the bacterium is widely used as a vector to genetically modify a wide range of organisms, including plants, yeasts, fungi, and algae. Var...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang Q,Li X,Tu H,Pan SQ

更新日期：2017-03-14 00:00:00

abstract：:Saccharopolyspora erythraea makes erythromycin, an antibiotic commonly used in human medicine. Unusually, the erythromycin biosynthetic (ery) cluster lacks a pathway-specific regulatory gene. We isolated a transcriptional regulator of the ery biosynthetic genes from S. erythraea and found that this protein appears to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chng C,Lum AM,Vroom JA,Kao CM

更新日期：2008-08-12 00:00:00

abstract：:Hypomagnesemia affects insulin resistance and is a risk factor for diabetes mellitus type 2 (DM2) and gestational diabetes mellitus (GDM). Two single nucleotide polymorphisms (SNPs) in the epithelial magnesium channel TRPM6 (V(1393)I, K(1584)E) were predicted to confer susceptibility for DM2. Here, we show using patch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nair AV,Hocher B,Verkaart S,van Zeeland F,Pfab T,Slowinski T,Chen YP,Schlingmann KP,Schaller A,Gallati S,Bindels RJ,Konrad M,Hoenderop JG

更新日期：2012-07-10 00:00:00

abstract：:In the T-cell somatic mutant J.CaM1, the T-cell antigen receptor complex is poorly coupled to the inositolphospholipid second messenger system; some antibodies against the invariant CD3 subunit of the receptor retain their agonist function in J.CaM1. Here we show by a combination of complementation assays that the mut...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldsmith MA,Dazin PF,Weiss A

更新日期：1988-11-01 00:00:00

abstract：:Cystic fibrosis (CF) is an autosomal recessive disorder, the most common lethal genetic disease in Caucasians. Respiratory disease is the major cause of morbidity and mortality. Indeed, 95% of CF patients die of respiratory failure. Pseudomonas aeruginosa, an opportunistic pathogen, chronically infects the lungs of ov...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li JD,Feng W,Gallup M,Kim JH,Gum J,Kim Y,Basbaum C

更新日期：1998-05-12 00:00:00

abstract：:Solid-state cross-polarization/magic-angle sample-spinning (13)C NMR spectra have been recorded on chlorophyll a-water aggregates, methyl pyrochlorophyllide a, and methyl pyropheophorbide a (derivatives that lack a phytyl chain). Spectra have also been collected under a decoupling regime in which resonances of certain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown CE,Spencer RB,Burger VT,Katz JJ

更新日期：1984-01-01 00:00:00

abstract：:Topological mechanical metamaterials are artificial structures whose unusual properties are protected very much like their electronic and optical counterparts. Here, we present an experimental and theoretical study of an active metamaterial--composed of coupled gyroscopes on a lattice--that breaks time-reversal symmet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nash LM,Kleckner D,Read A,Vitelli V,Turner AM,Irvine WT

更新日期：2015-11-24 00:00:00

abstract：:The amino acid sequence of rabbit skeletal muscle heat-stable inhibitor of the cAMP-dependent protein kinase has been determined by microsequencing techniques. Proof of the structure involved a series of nonoverlapping tryptic fragments for primary identification of 86% of the amino acids. Complementary fragments gene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scott JD,Fischer EH,Takio K,Demaille JG,Krebs EG

更新日期：1985-09-01 00:00:00

abstract：:We have used wild-type and variants of the T-lymphoma cell line S49 to explore internalization and down-regulation of adenylate cyclase-linked beta-adrenergic receptors. Internalization was defined by the loss in "surface receptors" detected at 4 degrees C on intact cells by the antagonists [3H]CGP-12177 or [125I]iodo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mahan LC,Koachman AM,Insel PA

更新日期：1985-01-01 00:00:00

abstract：:The spindle checkpoint ensures the accuracy of chromosome segregation during mitosis. The protein serine/threonine kinase, Mps1, is a critical component of the spindle checkpoint in human cells and regulates the kinetochore localization of key checkpoint proteins. The kinase activity of Mps1 is required for the spindl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang J,Chen Y,Zhao Y,Yu H

更新日期：2007-12-18 00:00:00

abstract：:Maltose-binding protein (MBP) is a two-domain protein that undergoes a ligand-mediated conformational rearrangement from an "open" to a "closed" structure on binding to maltooligosaccharides. To characterize the energy landscape associated with this transition, we have generated five variants of MBP with mutations loc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Millet O,Hudson RP,Kay LE

更新日期：2003-10-28 00:00:00

abstract：:Regulation of leukocyte integrin avidity is a crucial aspect of inflammation and immunity. The actin cytoskeleton has an important role in the regulation of integrin function, but the cytoskeletal proteins involved are largely unknown. Because inflammatory stimuli that activate integrin-mediated adhesion in human poly...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jones SL,Wang J,Turck CW,Brown EJ

更新日期：1998-08-04 00:00:00

abstract：:We analyzed variability in outer surface protein B (OspB) from Borrelia burgdorferi (Bb), the causative agent of Lyme disease, to determine how Bb escapes immune destruction. We have shown that vaccination with OspB from Bb strain B31 protected mice from infection with Bb B31 but not against Bb N40. The present study ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fikrig E,Tao H,Kantor FS,Barthold SW,Flavell RA

更新日期：1993-05-01 00:00:00

abstract：:Fertilization and certain later stages in mammalian embryonic development require fusion between membranes of individual cells. The mechanism of eukaryotic cell-cell fusion is unknown, and no surface molecules required for this process have been unequivocally identified. The role of the sperm surface protein fertilin ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Myles DG,Kimmel LH,Blobel CP,White JM,Primakoff P

更新日期：1994-05-10 00:00:00

abstract：:Small noncoding microRNAs (miRNAs) can contribute to cancer development and progression and are differentially expressed in normal tissues and cancers. From a large-scale miRnome analysis on 540 samples including lung, breast, stomach, prostate, colon, and pancreatic tumors, we identified a solid cancer miRNA signatur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Volinia S,Calin GA,Liu CG,Ambs S,Cimmino A,Petrocca F,Visone R,Iorio M,Roldo C,Ferracin M,Prueitt RL,Yanaihara N,Lanza G,Scarpa A,Vecchione A,Negrini M,Harris CC,Croce CM

更新日期：2006-02-14 00:00:00

abstract：:We have previously identified by cDNA cloning 5 mRNAs that appear in resting BALB/c 3T3 cells soon after growth stimulation by serum or platelet-derived growth factor. Five additional mRNAs of this class are described in this report. The mRNAs reached peak levels between 40 and 120 min after serum addition and rapidly...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lau LF,Nathans D

更新日期：1987-03-01 00:00:00

abstract：:Mechanism of photo-induced electron transfer and the subsequent hole transfer in DNA has been studied extensively, but so far we are not aware of any reliable report of the observation of the long-distance hole-transfer process. In this article, we demonstrate the results of direct observation for the long-distance ho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takada T,Kawai K,Fujitsuka M,Majima T

更新日期：2004-09-28 00:00:00

abstract：:We present here a brief review of direct force measurements between hydrophobic surfaces in aqueous solutions. For almost 70 years, researchers have attempted to understand the hydrophobic effect (the low solubility of hydrophobic solutes in water) and the hydrophobic interaction or force (the unusually strong attract...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meyer EE,Rosenberg KJ,Israelachvili J

更新日期：2006-10-24 00:00:00

abstract：:We have evaluated the effect of ligand binding on expression of the receptor for IgE on rat basophilic leukemia (RBL) cells. RBL cells were grown in the presence or absence of 131I-labeled IgE and sometimes were also surface labeled with 125I. We found that cells grown in the presence of IgE continued to accumulate re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Furuichi K,Rivera J,Isersky C

更新日期：1985-03-01 00:00:00

abstract：:Fluoroquinolones are antibacterial agents that attack DNA gyrase and topoisomerase IV on chromosomal DNA. The existence of two fluoroquinolone targets and stepwise accumulation of resistance suggested that new quinolones could be found that would require cells to obtain two topoisomerase mutations to display resistanc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao X,Xu C,Domagala J,Drlica K

更新日期：1997-12-09 00:00:00

abstract：:p150Glued was first identified as a polypeptide that copurifies with cytoplasmic dynein, the minus-end-directed microtubule-based motor protein, and has more recently been shown to be present as a member of the oligomeric dynactin complex, which includes the actin-related protein centractin (Arp-1). Dynactin is though...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Waterman-Storer CM,Karki S,Holzbaur EL

更新日期：1995-02-28 00:00:00