Direct observation of hole transfer through double-helical DNA over 100 A.

abstract：:The interaction between cells and nanostructured materials is attracting increasing interest, because of the possibility to open up novel concepts for the design of smart nanobiomaterials with active biological functionalities. In this frame we investigated the response of human neuroblastoma cell line (SH-SY5Y) to go...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brunetti V,Maiorano G,Rizzello L,Sorce B,Sabella S,Cingolani R,Pompa PP

更新日期：2010-04-06 00:00:00

abstract：:Because of the insolubility and polymeric properties of amyloid fibrils, techniques used conventionally to analyze protein structure and dynamics have often been hampered. Ultrasonication can induce the monomeric solution of amyloidogenic proteins to form amyloid fibrils. However, ultrasonication can break down prefor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chatani E,Lee YH,Yagi H,Yoshimura Y,Naiki H,Goto Y

更新日期：2009-07-07 00:00:00

abstract：:Data drawn from a global compilation of studies quantitatively confirm the long-articulated contention that erosion rates from conventionally plowed agricultural fields average 1-2 orders of magnitude greater than rates of soil production, erosion under native vegetation, and long-term geological erosion. The general ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Montgomery DR

更新日期：2007-08-14 00:00:00

abstract：:Soil bacteria and fungi play key roles in the functioning of terrestrial ecosystems, yet our understanding of their responses to climate change lags significantly behind that of other organisms. This gap in our understanding is particularly true for drylands, which occupy ∼41% of Earth´s surface, because no global, sy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maestre FT,Delgado-Baquerizo M,Jeffries TC,Eldridge DJ,Ochoa V,Gozalo B,Quero JL,García-Gómez M,Gallardo A,Ulrich W,Bowker MA,Arredondo T,Barraza-Zepeda C,Bran D,Florentino A,Gaitán J,Gutiérrez JR,Huber-Sannwald E,Jan

更新日期：2015-12-22 00:00:00

abstract：:The mechanism of the inotropic effect of cardiac glycosides on the heart has long been controversial. Inotropic effects at low concentrations of cardiac glycosides indicate more than one class of receptor or more than one cellular mechanism. In the brain of the rat, high- and low-affinity cardiac glycoside receptors h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sweadner KJ,Farshi SK

更新日期：1987-12-01 00:00:00

abstract：:The latent membrane protein (LMP1) of Epstein-Barr virus (EBV) is expressed in EBV-associated nasopharyngeal carcinoma, which is notoriously metastatic. Although it is established that LMP1 represses E-cadherin expression and enhances the invasive ability of carcinoma cells, the mechanism underlying this repression re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsai CN,Tsai CL,Tse KP,Chang HY,Chang YS

更新日期：2002-07-23 00:00:00

abstract：:Fluorescence in situ hybridization (FISH) is a powerful tool for physical mapping in human and other mammalian species. However, application of the FISH technique has been limited in plant species, especially for mapping single- or low-copy DNA sequences, due to inconsistent signal production in plant chromosome prepa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jiang J,Gill BS,Wang GL,Ronald PC,Ward DC

更新日期：1995-05-09 00:00:00

abstract：:Given the widely noted increase in the warming effects of rising greenhouse gas concentrations, it has been unclear why global surface temperatures did not rise between 1998 and 2008. We find that this hiatus in warming coincides with a period of little increase in the sum of anthropogenic and natural forcings. Declin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaufmann RK,Kauppi H,Mann ML,Stock JH

更新日期：2011-07-19 00:00:00

abstract：:Theories of density-dependent natural selection predict that evolution should favor those genotypes with the highest per capita rates of population growth under the current density conditions. These theories are silent about the mechanisms that may give rise to these increases in density-dependent growth rates. We hav...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo PZ,Mueller LD,Ayala FJ

更新日期：1991-12-01 00:00:00

abstract：:p63 inhibits metastasis. Here, we show that p63 (both TAp63 and ΔNp63 isoforms) regulates expression of miR-205 in prostate cancer (PCa) cells, and miR-205 is essential for the inhibitory effects of p63 on markers of epithelial-mesenchymal transition (EMT), such as ZEB1 and vimentin. Correspondingly, the inhibitory ef...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tucci P,Agostini M,Grespi F,Markert EK,Terrinoni A,Vousden KH,Muller PA,Dötsch V,Kehrloesser S,Sayan BS,Giaccone G,Lowe SW,Takahashi N,Vandenabeele P,Knight RA,Levine AJ,Melino G

更新日期：2012-09-18 00:00:00

abstract：:Less than 100 cis-regulatory DNAs have been characterized in the context of transgenic metazoan embryos. Here we investigate the feasibility of conducting a genome-wide search for tissue-specific enhancers in the ascidian Ciona intestinalis. A total of 138 random genomic DNA fragments with an average size of 1.7 kb we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harafuji N,Keys DN,Levine M

更新日期：2002-05-14 00:00:00

abstract：:We have shown that cellular inhibitor of apoptosis proteins (cIAPs) impair clearance of hepatitis B virus (HBV) infection by preventing TNF-mediated killing/death of infected cells. A key question, with profound therapeutic implications, is whether this finding can be translated to the development of drugs that promot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ebert G,Allison C,Preston S,Cooney J,Toe JG,Stutz MD,Ojaimi S,Baschuk N,Nachbur U,Torresi J,Silke J,Begley CG,Pellegrini M

更新日期：2015-05-05 00:00:00

abstract：:Endosomal cargo travels through a dynamic vesicle network en route to degradation by lysosomes or recycling through the Golgi apparatus back to the cell surface. Rab5 is a key determinant of the early endosomes by organizing effector proteins in specific subdomains and mediating early endosome fusion. We find that ear...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Antignani A,Youle RJ

更新日期：2008-06-10 00:00:00

abstract：:Preparations of the hemin-controlled repressor (HCR) from rabbit reticulocytes contain 3':5'-cyclic-AMP-independent protein kinase activity for the smallest subunit of the peptide initiation factor eIF-2 and for proteins of reticulocyte 40S ribosomal subunits. Binding of the ternary complex formed between Met-tRNAf, G...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kramer G,Henderson AB,Pinphanichakarn P,Wallis MH,Hardesty B

更新日期：1977-04-01 00:00:00

abstract：:Human transcription factor IIA (TFIIA) is composed of three subunits (alpha, beta, and gamma). TFIIA interacts with the TATA-box binding protein and can overcome repression of transcription. TFIIA was found to be necessary for VP16-mediated transcriptional activation through a coactivator function. We have separated t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： MA D,Olave I,Merino A,Reinberg D

更新日期：1996-06-25 00:00:00

abstract：:Humans have altered natural patterns of fire for millennia, but the impact of human-set fires is thought to have been slight in wet closed-canopy forests. In the South Island of New Zealand, Polynesians (Māori), who arrived 700-800 calibrated years (cal y) ago, and then Europeans, who settled ∼150 cal y ago, used fire...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McWethy DB,Whitlock C,Wilmshurst JM,McGlone MS,Fromont M,Li X,Dieffenbacher-Krall A,Hobbs WO,Fritz SC,Cook ER

更新日期：2010-12-14 00:00:00

abstract：:The global prevalence of prediabetes and type 2 diabetes (T2D) is increasing, and it is contributing to the susceptibility to diabetes and its related epidemic in offspring. Although the impacts of paternal impaired fasting blood glucose and glucose intolerance on the metabolism of offspring have been well established...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wei Y,Yang CR,Wei YP,Zhao ZA,Hou Y,Schatten H,Sun QY

更新日期：2014-02-04 00:00:00

abstract：:The chemokine CXC ligand 8 (CXCL8)/IL-8 and related agonists recruit and activate polymorphonuclear cells by binding the CXC chemokine receptor 1 (CXCR1) and CXCR2. Here we characterize the unique mode of action of a small-molecule inhibitor (Repertaxin) of CXCR1 and CXCR2. Structural and biochemical data are consiste...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bertini R,Allegretti M,Bizzarri C,Moriconi A,Locati M,Zampella G,Cervellera MN,Di Cioccio V,Cesta MC,Galliera E,Martinez FO,Di Bitondo R,Troiani G,Sabbatini V,D'Anniballe G,Anacardio R,Cutrin JC,Cavalieri B,Mainiero F

更新日期：2004-08-10 00:00:00

abstract：:The kinase Cdc2p is a central regulator of entry into and progression through nuclear division during mitosis and meiosis in eukaryotes. Cdc2p is activated at the onset of mitosis by dephosphorylation on tyrosine-15, the phosphorylation status of which is determined mainly by the kinase Wee1p and the phosphatase Cdc25...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murakami-Tonami Y,Yamada-Namikawa C,Tochigi A,Hasegawa N,Kojima H,Kunimatsu M,Nakanishi M,Murakami H

更新日期：2007-09-11 00:00:00

abstract：:We investigated the effects of beta-estradiol on the locomotor behavior of female mice in a radial maze. Data comprising the total distance traveled during each arm entry were obtained from video records of six consecutive daily recording sessions. Distributions of these data were bimodal for both ovariectomized contr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coop AD,Stavarache MA,Pfaff DW,Reeke GN

更新日期：2006-10-17 00:00:00

abstract：:Excellent electrophoretic separations of a variety of biological molecules can be accomplished by using uncharged, triblock copolymers as the "gel" media. These copolymers form uncrosslinked, lyotropic liquid crystalline phases of large micelles between which molecules must travel. Unlike crosslinked hydrogels in comm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rill RL,Locke BR,Liu Y,Van Winkle DH

更新日期：1998-02-17 00:00:00

abstract：:Conventional asymmetric PCR is inefficient and difficult to optimize because limiting the concentration of one primer lowers its melting temperature below the reaction annealing temperature. Linear-After-The-Exponential (LATE)-PCR describes a new paradigm for primer design that renders assays as efficient as symmetric...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sanchez JA,Pierce KE,Rice JE,Wangh LJ

更新日期：2004-02-17 00:00:00

abstract：:To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bauer S,Vasu P,Persson S,Mort AJ,Somerville CR

更新日期：2006-07-25 00:00:00

abstract：:The rhizobium-legume symbiosis has been widely studied as the model of mutualistic evolution and the essential component of sustainable agriculture. Extensive genetic and recent genomic studies have led to the hypothesis that many distinct strategies, regardless of rhizobial phylogeny, contributed to the varied rhizob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tian CF,Zhou YJ,Zhang YM,Li QQ,Zhang YZ,Li DF,Wang S,Wang J,Gilbert LB,Li YR,Chen WX

更新日期：2012-05-29 00:00:00

abstract：:Pertussis toxin (PT), a protein produced by Bordetella pertussis, was studied for its effect on lipolysis in isolated rat epididymal adipocytes. Exposure of adipocytes to pertussis toxin resulted in a significant increase in cyclic AMP levels and lipolysis after a lag of 1-2 hr. Both the maximal rate of lipolysis and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olansky L,Myers GA,Pohl SL,Hewlett EL

更新日期：1983-11-01 00:00:00

abstract：:Mucopolysaccharides have been isolated, fractionated, and characterized from the nuclei of cultured B16 mouse melanoma cells grown in the presence of (3-H)-glucosamine and (35-S)sulfate. Digestion of the nuclei with DNase followed by Pronase gave a mixture of complex carbohydrates from which the mucopolysaccharides we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhavanandan VP,Davidson EA

更新日期：1975-06-01 00:00:00

abstract：:The Escherichia coli Min system self-organizes into a cell-pole to cell-pole oscillator on the membrane to prevent divisions at the cell poles. Reconstituting the Min system on a lipid bilayer has contributed to elucidating the oscillatory mechanism. However, previous in vitro patterns were attained with protein densi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vecchiarelli AG,Li M,Mizuuchi M,Hwang LC,Seol Y,Neuman KC,Mizuuchi K

更新日期：2016-03-15 00:00:00

abstract：:Coagulation is an innate defense mechanism intended to limit blood loss and trap invading pathogens during infection. However, Staphylococcus aureus has the ability to hijack the coagulation cascade and generate clots via secretion of coagulases. Although many S. aureus have this characteristic, some do not. The popul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Trivedi U,Madsen JS,Everett J,Fell C,Russel J,Haaber J,Crosby HA,Horswill AR,Burmølle M,Rumbaugh KP,Sørensen SJ

更新日期：2018-12-11 00:00:00

abstract：:The state-to-state transfer of rotational and vibrational energy has been studied for S1 glyoxal (CHOCHO) in collisions with D2, N2, CO and C2H4 using crossed molecular beams. A laser is used to pump glyoxal seeded in He to its S1 zero point level with zero angular momentum about its top axis (K' = 0). The inelastic s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parmenter CS,Clegg SM,Krajnovich DJ,Lu S

更新日期：1997-08-05 00:00:00

abstract：:Molecular targeting of the two receptor interaction domains of the epigenetic repressor silencing mediator of retinoid and thyroid hormone receptors (SMRT(mRID)) produced a transplantable skeletal syndrome that reduced radial bone growth, increased numbers of bone-resorbing periosteal osteoclasts, and increased bone f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hong SH,Dvorak-Ewell M,Stevens HY,Barish GD,Castro GL,Nofsinger R,Frangos JA,Shoback D,Evans RM

更新日期：2013-11-19 00:00:00