Abstract：
:Interleukin 1b (IL-1b) is a member of the cytokine family that serves as major mediators for early pro-inflammatory responses. During artificial breeding of Pacific cod (Gadus macrocephalus), outbreaks of NNV in the early development stages could lead to high mortality. Recent research reported that IL-1b participated in the host-virus interaction against NNV infection, however, no IL-1b gene has been identified from Pacific cod so far. In this study, we described the identification and characterization of a IL-1b from Pacific cod transcriptome at both transcript and protein level. Sequence alignment and phylogenetic analysis were conducted to determine and confirm their evolutionary relationship. Expression analysis revealed that IL-1b was expressed in all tested tissues and the western blot analysis confirmed the same expression profiles using purified recombinant IL-1b protein. In addition, significant up-regulation was detected in IL-1b transcripts during early development stages at 30dph and 50dph. After challenged with poly I:C, IL-1b was significantly induced after 24 h. Taken together, these results strongly suggested that IL-1b might play an essential role in innate defense mechanism of Pacific cod.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Hu B,Chen B,Mao M,Chen M,Liu X,Cui Q,Liu Y,Jiang Cdoi
10.1016/j.dci.2018.07.025subject
Has Abstractpub_date
2018-11-01 00:00:00pages
213-218eissn
0145-305Xissn
1879-0089pii
S0145-305X(18)30248-9journal_volume
88pub_type
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