Abstract:
:Hemolytic activity in coelomic fluid of Eisenia fetida (ECF) is due to three proteins H1, H2, H3 with molecular weights of 46, 43 and 40 kD, respectively. These proteins were isolated by preparative PAGE. H1 and H2 were shown to be stable in SDS and alpha-2-ME whereas H3 splits into two fragments with molecular weights of 18 and 21 kD after SDS treatment. IEF indicates that each protein consists of different isoforms with pIs between 5.1 and 6.2 H3 was demonstrated to be a bifunctional protein that can lyse and agglutinate erythrocytes. At 56 degrees C hemolytic activity of all three proteins was inactivated, but the agglutination activity of H3 was stable. Intracoelomic injection of erythrocytes reduced the number of hemolysins from three to two. Monospecific antisera were raised against the isolated hemolysins H1,2 and 3. The use of these antibodies and of carbohydrates as inhibitors of the biological activity of the molecules demonstrates the close structural relationship of agglutinins and hemolysins in the CF of E. fetida.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Eue I,Kauschke E,Mohrig W,Cooper ELdoi
10.1016/s0145-305x(97)00049-9subject
Has Abstractpub_date
1998-01-01 00:00:00pages
13-25issue
1eissn
0145-305Xissn
1879-0089pii
S0145305X97000499journal_volume
22pub_type
杂志文章abstract::Bacterial challenge enhances phospholipase A(2) (PLA(2)) activity, which in turn induces biosynthesis of various eicosanoids that mediate non-self recognition signal to immune effectors in insects. But, there is little information on how PLA(2) activity is controlled after the non-self recognition. A recent genome ana...
journal_title:Developmental and comparative immunology
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journal_title:Developmental and comparative immunology
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journal_title:Developmental and comparative immunology
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journal_title:Developmental and comparative immunology
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journal_title:Developmental and comparative immunology
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journal_title:Developmental and comparative immunology
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journal_title:Developmental and comparative immunology
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