当前位置： SCI文献检索 > ATHEROSCLEROSIS期刊下所有文献 > Primary culture of endothelial cells from atherosclerotic human aorta. Part 1. Identification, morphological and ultrastructural characteristics of two endothelial cell subpopulations.

Primary culture of endothelial cells from atherosclerotic human aorta. Part 1. Identification, morphological and ultrastructural characteristics of two endothelial cell subpopulations.

Abstract：

:Endothelial cells (EC) were harvested by 0.1% collagenase treatment for adult human thoracic aortas obtained 1-3 h after sudden death. At least 35-70% of EC were removed from the intimal surface of aorta, 90-95% of them being viable. Plating efficiency was 70-80%. Monolayer formation was achieved at a seeding density of 5-8 X 10(2) cells/mm2. The cells were identified as endothelium by the presence of Factor VIII antigen, Weigel-Palade bodies and typically endothelial morphology at confluence. Unlike endothelial cultures derived from human umbilical veins and infant aortas, primary cultures obtained from human adult aortas contain multinuclear EC with Factor VIII antigen and Weibel-Palade bodies. The number of multinuclear EC in cultures isolated from aortas affected by atherosclerosis was 2-fold higher (P less than 0.05) than in cultures obtained from grossly normal aortas taken from donors of the same age. EC with numerous lipid inclusions revealed by oil-red-O staining were present in all the EC primary cultures derived from aortas affected by atherosclerosis. No oil-red-O-positive cells were detected among the EC cultured from infant aorta, aorta of young donors, and umbilical vein. An electron microscopic examination of EC from atherosclerotic aorta in culture and in situ failed to reveal any ultrastructural peculiarities distinguishing multinuclear EC from the mononuclear EC.

journal_name

Atherosclerosis

journal_title

Atherosclerosis

authors

Antonov AS,Nikolaeva MA,Klueva TS,Romanov YuA,Babaev VR,Bystrevskaya VB,Perov NA,Repin VS,Smirnov VN

doi

10.1016/0021-9150(86)90027-4

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

1-19

issue

1

eissn

0021-9150

issn

1879-1484

pii

0021-9150(86)90027-4

journal_volume

59

pub_type

杂志文章

相关文献

ATHEROSCLEROSIS文献大全
  • Non-saturable degradation of LDL by monocyte-derived macrophages leads to a reduction in HMG-CoA reductase activity with little synthesis of cholesteryl esters.

    abstract：:In monocyte-derived macrophages from both normal and familial hypercholesterolaemic (FH) subjects, degradation of low density lipoprotein (LDL) through non-saturable pathways produced the same fall in 3-hydroxy-3-methylglutaryl-CoA reductase activity as receptor-mediated degradation of acetylated LDL, yet did not lead...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/0021-9150(87)90238-3

    authors： Knight BL,Soutar AK,Patel DD

    更新日期：1987-04-01 00:00:00

  • Inhibitory effects of tranilast on expression of transforming growth factor-beta isoforms and receptors in injured arteries.

    abstract：:Tranilast (N(3,4-dimethoxycinnamoyl)anthranilic acid), an agent which in cell culture inhibits transforming growth factor-beta (TGF-beta) secretion and antagonises the effects of TGF-beta and platelet-derived growth factor (PDGF) on cell migration and proliferation, has been reported to reduce the incidence of resteno...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/s0021-9150(97)00275-x

    authors： Ward MR,Sasahara T,Agrotis A,Dilley RJ,Jennings GL,Bobik A

    更新日期：1998-04-01 00:00:00

  • Comprehensive assessment of cardiovascular structure and function and disease risk in middle-aged ultra-endurance athletes.

    abstract：BACKGROUND AND AIMS:Recent studies suggest that long-term endurance training may be damaging to the heart, thus increasing cardiovascular disease (CVD) risk. However, studies utilizing cardiac imaging are conflicting and lack measures of central and peripheral vascular structure and function, which are also independent...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/j.atherosclerosis.2020.11.030

    authors： Bachman NP,Terwoord JD,Richards JC,Braun B,Green CP,Luckasen GJ,Dinenno FA

    更新日期：2020-12-01 00:00:00

  • Cost-effectiveness of pravastatin in secondary prevention of coronary heart disease: comparison between Belgium and the United States of a projected risk model.

    abstract：:Methodological differences and variations in health care regulations among countries often preclude direct comparisons of cost-effectiveness studies. A projected risk model was applied, designed to determine the economic value in the United States of pravastatin in the secondary prevention of coronary heart disease (C...

    journal_title：Atherosclerosis

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s0021-9150(97)00321-3

    authors： Muls E,Van Ganse E,Closon MC

    更新日期：1998-04-01 00:00:00

  • MicroRNA-124-3p inhibits collagen synthesis in atherosclerotic plaques by targeting prolyl 4-hydroxylase subunit alpha-1 (P4HA1) in vascular smooth muscle cells.

    abstract：BACKGROUND AND AIMS:Collagen synthesis in vascular smooth muscle cells (VSMCs) is very important in atherosclerosis, as it affects plaque stability. In this study, we aim to assess whether miR-124-3p is involved in the collagen synthesis process in VSMCs and the role it might play in atherosclerotic development. METHO...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/j.atherosclerosis.2018.08.034

    authors： Chen W,Yu F,Di M,Li M,Chen Y,Zhang Y,Liu X,Huang X,Zhang M

    更新日期：2018-10-01 00:00:00

  • Changes of serum apolipoprotein levels after oral administration of fat in human subjects.

    abstract：:Changes of serum apolipoprotein levels were studied every hour for 6 h after the administration of 55 g butter to 8 healthy male subjects. The mean serum apo A-IV level was significantly increased at 4 h (P less than 0.05) after fat ingestion compared to the mean initial level, although no significant changes of level...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/0021-9150(88)90161-x

    authors： Seishima M,Noma A,Torizawa H,Muto Y

    更新日期：1988-09-01 00:00:00

  • Comparison of hypocholesterolemic effects induced by dietary linoleic acid and oleic acid in hamsters.

    abstract：:We investigated the differences between the hypocholesterolemic effects induced by dietary linoleic acid and those induced by oleic acid in hamsters. Addition of 5% linoleic acid or oleic acid to a 0.1% cholesterol-supplemented diet diminished the increases in plasma total and low density lipoprotein (LDL) cholesterol...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/0021-9150(94)05486-3

    authors： Kurushima H,Hayashi K,Toyota Y,Kambe M,Kajiyama G

    更新日期：1995-04-24 00:00:00

  • Pulse wave velocity is associated with early clinical outcome after ischemic stroke.

    abstract：BACKGROUND AND PURPOSES:Pulse wave analysis (PWV), a marker of aortic stiffness, has independent predictive value for cardiovascular morbidity and mortality in both healthy and high-risk populations, especially fatal stroke, and for long-term functional stroke prognosis. Whether arterial stiffness and wave reflection a...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/j.atherosclerosis.2012.09.024

    authors： Gąsecki D,Rojek A,Kwarciany M,Kowalczyk K,Boutouyrie P,Nyka W,Laurent S,Narkiewicz K

    更新日期：2012-12-01 00:00:00

  • Genetic and pharmacological inhibition of the 5-lipoxygenase/leukotriene pathway in atherosclerotic lesion development in ApoE deficient mice.

    abstract：:The 5-lipoxygenase (5-LO) catalyzed formation of leukotriene (LT) lipid mediators is a pathway contributing to inflammatory events in asthma and more recently has been associated with cardiovascular disease. However, the relative impact of this pathway in atherogenesis has been controversial and a variety of mixed res...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/j.atherosclerosis.2008.07.045

    authors： Cao RY,St Amand T,Gräbner R,Habenicht AJ,Funk CD

    更新日期：2009-04-01 00:00:00

  • Cross-sectional association of soluble thrombomodulin with mild peripheral artery disease; the ARIC study. Atherosclerosis Risk in Communities.

    abstract：:Thrombomodulin, an endothelial membrane glycoprotein, is an essential part of the protein C anti-coagulant pathway. It may also have a role in the regulation of fibrinolysis. We carried out a cross-sectional study to assess the association of soluble thrombomodulin (sTM) with peripheral artery disease (PAD) in a strat...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/s0021-9150(00)00729-2

    authors： Salomaa V,Matei C,Aleksic N,Sansores-Garcia L,Folsom AR,Juneja H,Park E,Wu KK

    更新日期：2001-08-01 00:00:00

  • BMP-2 promotes phosphate uptake, phenotypic modulation, and calcification of human vascular smooth muscle cells.

    abstract：:Vascular calcification is associated with increased risk of cardiovascular events that are the most common cause of death in patients with end-stage renal disease. Clinical and experimental studies indicate that hyperphosphatemia is a risk factor for vascular calcification and cardiovascular mortality in these patient...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/j.atherosclerosis.2007.11.031

    authors： Li X,Yang HY,Giachelli CM

    更新日期：2008-08-01 00:00:00

  • Aortic size and aortic calcification. A necropsy Study.

    abstract：:Measurements of aortic length and circumference in 336 post-mortem specimens confirm earlier, neglected observations on the progressive increase in aortic size which occurs with advancing years. The increase is not related to atherosclerosis , or to hypertension and seems to be part of a true ageing process. The value...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/0021-9150(77)90162-9

    authors： Mitchell JR,Adams JH

    更新日期：1977-08-01 00:00:00

  • Heightened inflammation is linked to carotid intima-media thickness and endothelial activation in HIV-infected children.

    abstract：OBJECTIVES:HIV+ patients are at increased risk of cardiovascular disease (CVD). Inflammation plays a role in adults, but has not yet been assessed in HIV+ children. We compared proinflammatory cytokines and adhesion molecules in HIV+ children versus healthy controls, and assessed their relationship to carotid intima-me...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/j.atherosclerosis.2010.04.008

    authors： Ross AC,O'Riordan MA,Storer N,Dogra V,McComsey GA

    更新日期：2010-08-01 00:00:00

  • Sulforaphane inhibits endothelial lipase expression through NF-κB in endothelial cells.

    abstract：OBJECTIVE:Endothelial lipase (EL) is a new member of triacylglycerol lipase family that has been shown to decrease high-density lipoprotein (HDL) cholesterol levels leading to increased risk of atherosclerosis. Its expression is increased during inflammation and by inflammatory cytokines. Sulforaphane (SFN) is a natura...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/j.atherosclerosis.2010.07.015

    authors： Kivelä AM,Mäkinen PI,Jyrkkänen HK,Mella-Aho E,Xia Y,Kansanen E,Leinonen H,Verma IM,Ylä-Herttuala S,Levonen AL

    更新日期：2010-11-01 00:00:00

  • A study of DNA polymorphism in the apolipoprotein B gene in a Japanese population.

    abstract：:A Japanese group comprising 53 hyperlipidemic and 54 normolipidemic subjects was genotyped for DNA restriction fragment length polymorphisms (RFLPs) at the apo B gene locus. The polymorphisms with XbaI and PvuII were present at allelic frequencies of 0.04 (X1 allele) and 0.96 (X2 allele), 0.94 (P1 allele) and 0.06 (P2...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/0021-9150(88)90064-0

    authors： Aburatani H,Matsumoto A,Itoh H,Yamada N,Murase T,Takaku F,Itakura H

    更新日期：1988-07-01 00:00:00

  • Inhibition of intimal thickening of the carotid artery of rabbits and of outgrowth of explants of aorta by probucol.

    abstract：:The effect of administration of probucol in preventing intimal thickening of rabbit carotid artery after balloon catheter injury and the mechanism of action of the drug were studied. Groups of 6 male New Zealand-White rabbits were given normal diet (Group I), high cholesterol diet (Group II) or high cholesterol diet p...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/0021-9150(92)90127-3

    authors： Shinomiya M,Shirai K,Saito Y,Yoshida S

    更新日期：1992-12-01 00:00:00

  • Further studies on serum lipoproteins in connective tissue diseases.

    abstract：:Men with ankylosing spondylitis (AS) and females with systemic lupus erythematosus (SLE) were found to have low total serum lipid concentrations similar to results previously obtained in patients with rheumatoid arthritis (RA). In AS men total serum TG was about 50% of control values and in AS men and SLE women total ...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/0021-9150(78)90041-2

    authors： Rössner S

    更新日期：1978-09-01 00:00:00

  • Long-chain omega-3 fatty acids, fibrates and niacin as therapeutic options in the treatment of hypertriglyceridemia: a review of the literature.

    abstract：:Hypertriglyceridemia affects approximately 33% of the US population. Elevated triglyceride levels are independently associated with cardiovascular disease (CVD) risk, and severe hypertriglyceridemia is a risk factor for acute pancreatitis. Guidelines for the management of severe hypertriglyceridemia (≥5.6 mmol/L [≥500...

    journal_title：Atherosclerosis

    pub_type： 杂志文章,评审

    doi：10.1016/j.atherosclerosis.2015.06.012

    authors： Ito MK

    更新日期：2015-10-01 00:00:00

  • Predictive value of preprocedural fibrinogen concerning coronary stenting.

    abstract：:Elevated fibrinogen levels after coronary balloon angioplasty have been reported to be useful in predicting restenosis. Therefore, we sought to evaluate the relationship between preprocedural fibrinogen levels and the 6-12-month outcomes of patients undergoing coronary stenting. Plasma levels of fibrinogen were measur...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/s0021-9150(02)00177-6

    authors： Otsuka M,Hayashi Y,Ueda H,Imazu M,Kohno N

    更新日期：2002-10-01 00:00:00

  • Involvement of MAP kinases in the control of cPLA(2) and arachidonic acid release in endothelial cells.

    abstract：:Cytosolic Phospholipase A(2) (cPLA(2)) has been implicated in receptor-mediated release of arachidonic acid from membrane phospholipids, the limiting step in prostacyclin and other eicosanoid production. Its activity is controlled by Ca(++) levels and enzymatically regulated phosphorylation. The purpose of this study ...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/s0021-9150(00)00631-6

    authors： Gudmundsdóttir IJ,Halldórsson H,Magnúsdóttir K,Thorgeirsson G

    更新日期：2001-05-01 00:00:00

  • Circulating oxidized LDL levels, current smoking and obesity in postmenopausal women.

    abstract：OBJECTIVE:The aim of the present study was to estimate circulating oxidized low-density lipoprotein (oxLDL) levels in postmenopausal women and evaluate their association with obesity and smoking status. DESIGN AND METHODS:The study included 135 postmenopausal women aged 52-75 years. Forty of them were overweight (BMI ...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/j.atherosclerosis.2008.11.006

    authors： Kassi E,Dalamaga M,Faviou E,Hroussalas G,Kazanis K,Nounopoulos Ch,Dionyssiou-Asteriou A

    更新日期：2009-07-01 00:00:00

  • Lower bone mass is associated with subclinical atherosclerosis, endothelial dysfunction and carotid thickness in the very elderly.

    abstract：BACKGROUND AND AIMS:Osteoporosis and coronary heart disease (CHD) are very common conditions among elderly people, and both represent a public health concern due to their prognostic consequences. Osteoporosis and CHD share many risk factors and pathophysiological mechanisms, such as calcification pathways. Clinical evi...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/j.atherosclerosis.2019.11.007

    authors： Campos-Staffico AM,Freitas WM,Carvalho LSF,Coelho-Filho OR,Nadruz W Jr,Oliveira RB,Sposito AC,Brasilia Study on Healthy Aging and Brasilia Heart Study.

    更新日期：2020-01-01 00:00:00

  • Efficacy and safety of adding alirocumab to rosuvastatin versus adding ezetimibe or doubling the rosuvastatin dose in high cardiovascular-risk patients: The ODYSSEY OPTIONS II randomized trial.

    abstract：OBJECTIVE:To compare lipid-lowering efficacy of adding alirocumab to rosuvastatin versus other treatment strategies (NCT01730053). METHODS:Patients receiving baseline rosuvastatin regimens (10 or 20 mg) were randomized to: add-on alirocumab 75 mg every-2-weeks (Q2W) (1-mL subcutaneous injection via pre-filled pen); ad...

    journal_title：Atherosclerosis

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1016/j.atherosclerosis.2015.11.010

    authors： Farnier M,Jones P,Severance R,Averna M,Steinhagen-Thiessen E,Colhoun HM,Du Y,Hanotin C,Donahue S

    更新日期：2016-01-01 00:00:00

  • A novel antioxidant 3,7-dihydroxy-isoflav-3-ene (DHIF) inhibits neointimal hyperplasia after vessel injury attenuating reactive oxygen species and nuclear factor-kappaB signaling.

    abstract：:Reactive oxygen species (ROS) contribute to neointimal smooth muscle proliferation by yet to be defined mechanisms. We examined the effects of a novel isoflavone 3,7-dihydroxy-isoflav-3-ene (DHIF) on development of neointimal lesions in relation to ROS elevations and cell signaling in injured arteries. Carotid arterie...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/j.atherosclerosis.2008.09.005

    authors： Kanellakis P,Pomilio G,Walker C,Husband A,Huang JL,Nestel P,Agrotis A,Bobik A

    更新日期：2009-05-01 00:00:00

  • The fractional catabolic rate of low density lipoprotein in normal individuals is influenced by variation in the apolipoprotein B gene: a preliminary study.

    abstract：:In a random sample of 22 normolipidaemic male Caucasian individuals, 35-49 years old, homozygosity for the X2 allele (cutting site) of the XbaI RFLP of the apo B gene was associated with higher mean total cholesterol and LDL-cholesterol concentration. These individuals also had significantly lower LDL fractional catab...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/0021-9150(88)90305-x

    authors： Houlston RS,Turner PR,Revill J,Lewis B,Humphries SE

    更新日期：1988-05-01 00:00:00

  • Differential effect of soluble intercellular adhesion molecule-1 on the progression of atherosclerosis as compared to arterial thrombosis: a prospective analysis of the Women's Health Study.

    abstract：BACKGROUND:Soluble intercellular adhesion molecule-1 (sICAM-1) is a transmembrane protein involved in the migration and adhesion of leukocytes to the vascular endothelium. While some studies indicate that elevated baseline sICAM-1 levels predict cardiovascular events, most of these studies were done in men; moreover, u...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/j.atherosclerosis.2007.04.051

    authors： Albert MA,Glynn RJ,Buring JE,Ridker PM

    更新日期：2008-03-01 00:00:00

  • Possible involvement of p44/p42 MAP kinase in retinoic acid-stimulated vascular endothelial growth factor release in aortic smooth muscle cells.

    abstract：:Retinoic acid modulates cell growth and differentiation of the vascular system. Vascular endothelial growth factor (VEGF) is known as a vascular permeability factor and a potent mitogen for vascular endothelial cells. In the present study, we investigated whether retinoic acid induces VEGF release in aortic smooth mus...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/j.atherosclerosis.2004.05.002

    authors： Tanabe K,Hirade K,Ishisaki A,Shu E,Suga H,Kitajima Y,Katagiri Y,Dohi S,Kozawa O

    更新日期：2004-08-01 00:00:00

  • Biosynthesis of aortic collagen and glycosaminoglycan following immunological injury.

    abstract：:The effect of immunological injury upon the early in vivo changes in aortic connective tissue metabolism was studied. It was found that a single antigen (bovine serum albumin), when administered in multiple doses, activated collagen synthesis and increased the rate of lgycosaminoglycan synthesis in the arterial wall. ...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/0021-9150(79)90003-0

    authors： Rokosova B,Bentley JP

    更新日期：1979-04-01 00:00:00

  • New atherosclerosis risk factors in obese, hypertensive and diabetic children and adolescents.

    abstract：UNLABELLED:In the last few years it has been proved that risk factors for atherosclerosis are present in children and adolescents, and that already at this early age they are connected with anatomic, atheromatous changes in vessels. These changes can not be fully explained as occurring in young people exhibiting tradit...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/s0021-9150(03)00003-0

    authors： Glowinska B,Urban M,Koput A,Galar M

    更新日期：2003-04-01 00:00:00

  • Atherosclerosis prevention by a fish oil-rich diet in apoE(-/-) mice is associated with a reduction of endothelial adhesion molecules.

    abstract：:Dietary intake of long-chain n-3 polyunsaturated fatty acids (PUFA) reduces the risk for atherosclerosis. Here we examine the effect of a fish oil (FO)-rich diet on the development of atherosclerotic lesions in apolipoprotein E-deficient (apoE(-/-)) mice, which are vulnerable because of their high plasma cholesterol a...

    journal_title：Atherosclerosis

    pub_type： 杂志文章

    doi：10.1016/j.atherosclerosis.2008.02.033

    authors： Casós K,Sáiz MP,Ruiz-Sanz JI,Mitjavila MT

    更新日期：2008-12-01 00:00:00